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P76143

- LSRF_ECOLI

UniProt

P76143 - LSRF_ECOLI

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Protein

Uncharacterized aldolase LsrF

Gene

lsrF

Organism
Escherichia coli (strain K12)
Status
Reviewed - Annotation score: 2 out of 5- Experimental evidence at protein leveli

Functioni

Could be involved in the degradation of phospho-AI-2, thereby terminating induction of the lsr operon and closing the AI-2 signaling cycle.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei203 – 2031Schiff-base intermediate with substrateBy similarity

GO - Molecular functioni

  1. lyase activity Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Lyase

Keywords - Ligandi

Schiff base

Enzyme and pathway databases

BioCyciEcoCyc:G6804-MONOMER.
ECOL316407:JW1510-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Uncharacterized aldolase LsrF (EC:4.1.2.-)
Gene namesi
Name:lsrF
Synonyms:yneB
Ordered Locus Names:b1517, JW1510
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
ProteomesiUP000000318: Chromosome, UP000000625: Chromosome

Organism-specific databases

EcoGeneiEG13810. lsrF.

Subcellular locationi

Cytoplasm Curated

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 291291Uncharacterized aldolase LsrFPRO_0000138945Add
BLAST

Proteomic databases

PaxDbiP76143.
PRIDEiP76143.

Expressioni

Inductioni

In the absence of AI-2, repressed by LsrR. Induced by AI-2, via release of the LsrR repressor. In the absence of glucose, induced by cAMP-CRP by direct binding to the upstream region of the lsr promoter.2 Publications

Gene expression databases

GenevestigatoriP76143.

Interactioni

Protein-protein interaction databases

DIPiDIP-12754N.
IntActiP76143. 5 interactions.
STRINGi511145.b1517.

Structurei

Secondary structure

1
291
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi34 – 4310Combined sources
Turni46 – 483Combined sources
Beta strandi51 – 555Combined sources
Helixi59 – 624Combined sources
Helixi71 – 744Combined sources
Helixi76 – 816Combined sources
Beta strandi83 – 875Combined sources
Helixi89 – 957Combined sources
Helixi98 – 1003Combined sources
Beta strandi104 – 1074Combined sources
Beta strandi109 – 1113Combined sources
Helixi127 – 1326Combined sources
Beta strandi136 – 1427Combined sources
Helixi149 – 16416Combined sources
Turni165 – 1673Combined sources
Beta strandi170 – 1745Combined sources
Helixi184 – 19613Combined sources
Beta strandi200 – 2056Combined sources
Turni208 – 2103Combined sources
Helixi211 – 2166Combined sources
Beta strandi222 – 2254Combined sources
Helixi232 – 24413Combined sources
Beta strandi248 – 2536Combined sources
Helixi254 – 2574Combined sources
Beta strandi259 – 2613Combined sources
Helixi262 – 27514Combined sources
Helixi279 – 2879Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3GKFX-ray2.90A/B/C/D/E/F/G/H/I/J/K/L/M/N/O/P/Q/R/S/T1-291[»]
3GLCX-ray2.50A/B/C/D/E/F/G/H/I/J/K/L/M/N/O/P/Q/R/S/T1-291[»]
3GNDX-ray2.90A/B/C/D/E/F/G/H/I/J/K/L/M/N/O/P/Q/R/S/T1-291[»]
4P2VX-ray2.51A/B/C/D/E/F/G/H/I/K1-291[»]
ProteinModelPortaliP76143.
SMRiP76143. Positions 10-290.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP76143.

Family & Domainsi

Sequence similaritiesi

Belongs to the DeoC/FbaB aldolase family.Curated

Phylogenomic databases

eggNOGiCOG1830.
HOGENOMiHOG000224792.
InParanoidiP76143.
KOiK08321.
OMAiGTRYGMP.
OrthoDBiEOG6Z6FRM.
PhylomeDBiP76143.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
InterProiIPR013785. Aldolase_TIM.
IPR002915. DeoC/FbaB/lacD_aldolase.
[Graphical view]
PfamiPF01791. DeoC. 1 hit.
[Graphical view]
PIRSFiPIRSF038992. Aldolase_Ia. 1 hit.

Sequencei

Sequence statusi: Complete.

P76143-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MADLDDIKDG KDFRTDQPQK NIPFTLKGCG ALDWGMQSRL SRIFNPKTGK
60 70 80 90 100
TVMLAFDHGY FQGPTTGLER IDINIAPLFE HADVLMCTRG ILRSVVPPAT
110 120 130 140 150
NRPVVLRASG ANSILAELSN EAVALSMDDA VRLNSCAVAA QVYIGSEYEH
160 170 180 190 200
QSIKNIIQLV DAGMKVGMPT MAVTGVGKDM VRDQRYFSLA TRIAAEMGAQ
210 220 230 240 250
IIKTYYVEKG FERIVAGCPV PIVIAGGKKL PEREALEMCW QAIDQGASGV
260 270 280 290
DMGRNIFQSD HPVAMMKAVQ AVVHHNETAD RAYELYLSEK Q
Length:291
Mass (Da):31,893
Last modified:February 1, 1997 - v1
Checksum:iD5CC245A5C709F1C
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00096 Genomic DNA. Translation: AAC74590.1.
AP009048 Genomic DNA. Translation: BAE76457.1.
PIRiH64905.
RefSeqiNP_416034.1. NC_000913.3.
YP_489780.1. NC_007779.1.

Genome annotation databases

EnsemblBacteriaiAAC74590; AAC74590; b1517.
BAE76457; BAE76457; BAE76457.
GeneIDi12932356.
946071.
KEGGiecj:Y75_p1492.
eco:b1517.
PATRICi32118330. VBIEscCol129921_1585.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00096 Genomic DNA. Translation: AAC74590.1 .
AP009048 Genomic DNA. Translation: BAE76457.1 .
PIRi H64905.
RefSeqi NP_416034.1. NC_000913.3.
YP_489780.1. NC_007779.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
3GKF X-ray 2.90 A/B/C/D/E/F/G/H/I/J/K/L/M/N/O/P/Q/R/S/T 1-291 [» ]
3GLC X-ray 2.50 A/B/C/D/E/F/G/H/I/J/K/L/M/N/O/P/Q/R/S/T 1-291 [» ]
3GND X-ray 2.90 A/B/C/D/E/F/G/H/I/J/K/L/M/N/O/P/Q/R/S/T 1-291 [» ]
4P2V X-ray 2.51 A/B/C/D/E/F/G/H/I/K 1-291 [» ]
ProteinModelPortali P76143.
SMRi P76143. Positions 10-290.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

DIPi DIP-12754N.
IntActi P76143. 5 interactions.
STRINGi 511145.b1517.

Proteomic databases

PaxDbi P76143.
PRIDEi P76143.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAC74590 ; AAC74590 ; b1517 .
BAE76457 ; BAE76457 ; BAE76457 .
GeneIDi 12932356.
946071.
KEGGi ecj:Y75_p1492.
eco:b1517.
PATRICi 32118330. VBIEscCol129921_1585.

Organism-specific databases

EchoBASEi EB3571.
EcoGenei EG13810. lsrF.

Phylogenomic databases

eggNOGi COG1830.
HOGENOMi HOG000224792.
InParanoidi P76143.
KOi K08321.
OMAi GTRYGMP.
OrthoDBi EOG6Z6FRM.
PhylomeDBi P76143.

Enzyme and pathway databases

BioCyci EcoCyc:G6804-MONOMER.
ECOL316407:JW1510-MONOMER.

Miscellaneous databases

EvolutionaryTracei P76143.
PROi P76143.

Gene expression databases

Genevestigatori P76143.

Family and domain databases

Gene3Di 3.20.20.70. 1 hit.
InterProi IPR013785. Aldolase_TIM.
IPR002915. DeoC/FbaB/lacD_aldolase.
[Graphical view ]
Pfami PF01791. DeoC. 1 hit.
[Graphical view ]
PIRSFi PIRSF038992. Aldolase_Ia. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  2. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
    Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
    Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  3. "Regulation of uptake and processing of the quorum-sensing autoinducer AI-2 in Escherichia coli."
    Xavier K.B., Bassler B.L.
    J. Bacteriol. 187:238-248(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: INDUCTION.
    Strain: K12 / MG1655 / ATCC 47076.
  4. "Cyclic AMP (cAMP) and cAMP receptor protein influence both synthesis and uptake of extracellular autoinducer 2 in Escherichia coli."
    Wang L., Hashimoto Y., Tsao C.-Y., Valdes J.J., Bentley W.E.
    J. Bacteriol. 187:2066-2076(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: INDUCTION.
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.

Entry informationi

Entry nameiLSRF_ECOLI
AccessioniPrimary (citable) accession number: P76143
Secondary accession number(s): Q2MB99
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: February 1, 1997
Last modified: November 26, 2014
This is version 96 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3