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P76143

- LSRF_ECOLI

UniProt

P76143 - LSRF_ECOLI

Protein

Uncharacterized aldolase LsrF

Gene

lsrF

Organism
Escherichia coli (strain K12)
Status
Reviewed - Annotation score: 2 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 94 (01 Oct 2014)
      Sequence version 1 (01 Feb 1997)
      Previous versions | rss
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    Functioni

    Could be involved in the degradation of phospho-AI-2, thereby terminating induction of the lsr operon and closing the AI-2 signaling cycle.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei203 – 2031Schiff-base intermediate with substrateBy similarity

    GO - Molecular functioni

    1. lyase activity Source: UniProtKB-KW

    Keywords - Molecular functioni

    Lyase

    Keywords - Ligandi

    Schiff base

    Enzyme and pathway databases

    BioCyciEcoCyc:G6804-MONOMER.
    ECOL316407:JW1510-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Uncharacterized aldolase LsrF (EC:4.1.2.-)
    Gene namesi
    Name:lsrF
    Synonyms:yneB
    Ordered Locus Names:b1517, JW1510
    OrganismiEscherichia coli (strain K12)
    Taxonomic identifieri83333 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
    ProteomesiUP000000318: Chromosome, UP000000625: Chromosome

    Organism-specific databases

    EcoGeneiEG13810. lsrF.

    Subcellular locationi

    Cytoplasm Curated

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 291291Uncharacterized aldolase LsrFPRO_0000138945Add
    BLAST

    Proteomic databases

    PaxDbiP76143.
    PRIDEiP76143.

    Expressioni

    Inductioni

    In the absence of AI-2, repressed by LsrR. Induced by AI-2, via release of the LsrR repressor. In the absence of glucose, induced by cAMP-CRP by direct binding to the upstream region of the lsr promoter.2 Publications

    Gene expression databases

    GenevestigatoriP76143.

    Interactioni

    Protein-protein interaction databases

    DIPiDIP-12754N.
    IntActiP76143. 5 interactions.
    STRINGi511145.b1517.

    Structurei

    Secondary structure

    1
    291
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi34 – 4310
    Turni46 – 483
    Beta strandi51 – 555
    Helixi59 – 624
    Helixi71 – 744
    Helixi76 – 816
    Beta strandi83 – 875
    Helixi89 – 957
    Helixi98 – 1003
    Beta strandi104 – 1074
    Beta strandi109 – 1113
    Helixi127 – 1326
    Beta strandi136 – 1427
    Helixi149 – 16416
    Turni165 – 1673
    Beta strandi170 – 1745
    Helixi184 – 19613
    Beta strandi200 – 2056
    Turni208 – 2103
    Helixi211 – 2166
    Beta strandi222 – 2254
    Helixi232 – 24413
    Beta strandi248 – 2536
    Helixi254 – 2574
    Beta strandi259 – 2613
    Helixi262 – 27514
    Helixi279 – 2879

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    3GKFX-ray2.90A/B/C/D/E/F/G/H/I/J/K/L/M/N/O/P/Q/R/S/T1-291[»]
    3GLCX-ray2.50A/B/C/D/E/F/G/H/I/J/K/L/M/N/O/P/Q/R/S/T1-291[»]
    3GNDX-ray2.90A/B/C/D/E/F/G/H/I/J/K/L/M/N/O/P/Q/R/S/T1-291[»]
    ProteinModelPortaliP76143.
    SMRiP76143. Positions 10-290.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP76143.

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the DeoC/FbaB aldolase family.Curated

    Phylogenomic databases

    eggNOGiCOG1830.
    HOGENOMiHOG000224792.
    KOiK08321.
    OMAiGTRYGMP.
    OrthoDBiEOG6Z6FRM.
    PhylomeDBiP76143.

    Family and domain databases

    Gene3Di3.20.20.70. 1 hit.
    InterProiIPR013785. Aldolase_TIM.
    IPR002915. DeoC/FbaB/lacD_aldolase.
    [Graphical view]
    PfamiPF01791. DeoC. 1 hit.
    [Graphical view]
    PIRSFiPIRSF038992. Aldolase_Ia. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    P76143-1 [UniParc]FASTAAdd to Basket

    « Hide

    MADLDDIKDG KDFRTDQPQK NIPFTLKGCG ALDWGMQSRL SRIFNPKTGK    50
    TVMLAFDHGY FQGPTTGLER IDINIAPLFE HADVLMCTRG ILRSVVPPAT 100
    NRPVVLRASG ANSILAELSN EAVALSMDDA VRLNSCAVAA QVYIGSEYEH 150
    QSIKNIIQLV DAGMKVGMPT MAVTGVGKDM VRDQRYFSLA TRIAAEMGAQ 200
    IIKTYYVEKG FERIVAGCPV PIVIAGGKKL PEREALEMCW QAIDQGASGV 250
    DMGRNIFQSD HPVAMMKAVQ AVVHHNETAD RAYELYLSEK Q 291
    Length:291
    Mass (Da):31,893
    Last modified:February 1, 1997 - v1
    Checksum:iD5CC245A5C709F1C
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U00096 Genomic DNA. Translation: AAC74590.1.
    AP009048 Genomic DNA. Translation: BAE76457.1.
    PIRiH64905.
    RefSeqiNP_416034.1. NC_000913.3.
    YP_489780.1. NC_007779.1.

    Genome annotation databases

    EnsemblBacteriaiAAC74590; AAC74590; b1517.
    BAE76457; BAE76457; BAE76457.
    GeneIDi12932356.
    946071.
    KEGGiecj:Y75_p1492.
    eco:b1517.
    PATRICi32118330. VBIEscCol129921_1585.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U00096 Genomic DNA. Translation: AAC74590.1 .
    AP009048 Genomic DNA. Translation: BAE76457.1 .
    PIRi H64905.
    RefSeqi NP_416034.1. NC_000913.3.
    YP_489780.1. NC_007779.1.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    3GKF X-ray 2.90 A/B/C/D/E/F/G/H/I/J/K/L/M/N/O/P/Q/R/S/T 1-291 [» ]
    3GLC X-ray 2.50 A/B/C/D/E/F/G/H/I/J/K/L/M/N/O/P/Q/R/S/T 1-291 [» ]
    3GND X-ray 2.90 A/B/C/D/E/F/G/H/I/J/K/L/M/N/O/P/Q/R/S/T 1-291 [» ]
    ProteinModelPortali P76143.
    SMRi P76143. Positions 10-290.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    DIPi DIP-12754N.
    IntActi P76143. 5 interactions.
    STRINGi 511145.b1517.

    Proteomic databases

    PaxDbi P76143.
    PRIDEi P76143.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAC74590 ; AAC74590 ; b1517 .
    BAE76457 ; BAE76457 ; BAE76457 .
    GeneIDi 12932356.
    946071.
    KEGGi ecj:Y75_p1492.
    eco:b1517.
    PATRICi 32118330. VBIEscCol129921_1585.

    Organism-specific databases

    EchoBASEi EB3571.
    EcoGenei EG13810. lsrF.

    Phylogenomic databases

    eggNOGi COG1830.
    HOGENOMi HOG000224792.
    KOi K08321.
    OMAi GTRYGMP.
    OrthoDBi EOG6Z6FRM.
    PhylomeDBi P76143.

    Enzyme and pathway databases

    BioCyci EcoCyc:G6804-MONOMER.
    ECOL316407:JW1510-MONOMER.

    Miscellaneous databases

    EvolutionaryTracei P76143.
    PROi P76143.

    Gene expression databases

    Genevestigatori P76143.

    Family and domain databases

    Gene3Di 3.20.20.70. 1 hit.
    InterProi IPR013785. Aldolase_TIM.
    IPR002915. DeoC/FbaB/lacD_aldolase.
    [Graphical view ]
    Pfami PF01791. DeoC. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF038992. Aldolase_Ia. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / MG1655 / ATCC 47076.
    2. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
      Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
      Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
    3. "Regulation of uptake and processing of the quorum-sensing autoinducer AI-2 in Escherichia coli."
      Xavier K.B., Bassler B.L.
      J. Bacteriol. 187:238-248(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: INDUCTION.
      Strain: K12 / MG1655 / ATCC 47076.
    4. "Cyclic AMP (cAMP) and cAMP receptor protein influence both synthesis and uptake of extracellular autoinducer 2 in Escherichia coli."
      Wang L., Hashimoto Y., Tsao C.-Y., Valdes J.J., Bentley W.E.
      J. Bacteriol. 187:2066-2076(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: INDUCTION.
      Strain: K12 / W3110 / ATCC 27325 / DSM 5911.

    Entry informationi

    Entry nameiLSRF_ECOLI
    AccessioniPrimary (citable) accession number: P76143
    Secondary accession number(s): Q2MB99
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1997
    Last sequence update: February 1, 1997
    Last modified: October 1, 2014
    This is version 94 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. Escherichia coli
      Escherichia coli (strain K12): entries and cross-references to EcoGene
    2. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3