Uncharacterized aldolase LsrF - Escherichia coli (strain K12)

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P76143 (LSRF_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 87. History...

Clusters with 100%, 90%, 50% identity |

Documents (3) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Uncharacterized aldolase LsrF
EC=4.1.2.-

Gene names

Name:	lsrF
Synonyms:	yneB
Ordered Locus Names:	b1517, JW1510

Organism

Escherichia coli (strain K12) [Reference proteome] [HAMAP]

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia ›

Protein attributes

Sequence length	291 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Could be involved in the degradation of phospho-AI-2, thereby terminating induction of the lsr operon and closing the AI-2 signaling cycle By similarity.
Subcellular location	Cytoplasm Potential.
Induction	In the absence of AI-2, repressed by LsrR. Induced by AI-2, via release of the LsrR repressor. In the absence of glucose, induced by cAMP-CRP by direct binding to the upstream region of the lsr promoter. Ref.3 Ref.4
Sequence similarities	Belongs to the DeoC/FbaB aldolase family.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	Schiff base
Molecular function	Lyase
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	metabolic process Inferred from electronic annotation. Source: GOC
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	lyase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 291

291

Uncharacterized aldolase LsrF

PRO_0000138945

Sites

Active site

203

Schiff-base intermediate with substrate By similarity

Secondary structure

291

Helix Strand Turn

Details...

Sequences

Sequence

Length

Mass (Da)

Tools

P76143 [UniParc].

Last modified February 1, 1997. Version 1.
Checksum: D5CC245A5C709F1C
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FASTA

291

31,893

        10         20         30         40         50         60 
MADLDDIKDG KDFRTDQPQK NIPFTLKGCG ALDWGMQSRL SRIFNPKTGK TVMLAFDHGY 

        70         80         90        100        110        120 
FQGPTTGLER IDINIAPLFE HADVLMCTRG ILRSVVPPAT NRPVVLRASG ANSILAELSN 

       130        140        150        160        170        180 
EAVALSMDDA VRLNSCAVAA QVYIGSEYEH QSIKNIIQLV DAGMKVGMPT MAVTGVGKDM 

       190        200        210        220        230        240 
VRDQRYFSLA TRIAAEMGAQ IIKTYYVEKG FERIVAGCPV PIVIAGGKKL PEREALEMCW 

       250        260        270        280        290 
QAIDQGASGV DMGRNIFQSD HPVAMMKAVQ AVVHHNETAD RAYELYLSEK Q

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[2]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[3]	"Regulation of uptake and processing of the quorum-sensing autoinducer AI-2 in Escherichia coli." Xavier K.B., Bassler B.L. J. Bacteriol. 187:238-248(2005) [PubMed] [Europe PMC] [Abstract] Cited for: INDUCTION. Strain: K12 / MG1655 / ATCC 47076.
[4]	"Cyclic AMP (cAMP) and cAMP receptor protein influence both synthesis and uptake of extracellular autoinducer 2 in Escherichia coli." Wang L., Hashimoto Y., Tsao C.-Y., Valdes J.J., Bentley W.E. J. Bacteriol. 187:2066-2076(2005) [PubMed] [Europe PMC] [Abstract] Cited for: INDUCTION. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

U00096 Genomic DNA. Translation: AAC74590.1.
AP009048 Genomic DNA. Translation: BAE76457.1.

PIR

H64905.

RefSeq

NP_416034.1. NC_000913.2.
YP_489780.1. NC_007779.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
3GKF	X-ray	2.90	A/B/C/D/E/F/G/H/I/J/K/L/M/N/O/P/Q/R/S/T	1-291	[»]
3GLC	X-ray	2.50	A/B/C/D/E/F/G/H/I/J/K/L/M/N/O/P/Q/R/S/T	1-291	[»]
3GND	X-ray	2.90	A/B/C/D/E/F/G/H/I/J/K/L/M/N/O/P/Q/R/S/T	1-291	[»]

ProteinModelPortal

P76143.

SMR

P76143. Positions 10-290.

ModBase

Search...

Protein-protein interaction databases

DIP

DIP-12754N.

IntAct

P76143. 3 interactions.

STRING

511145.b1517.

Proteomic databases

PaxDb

P76143.

PRIDE

P76143.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

EnsemblBacteria

AAC74590; AAC74590; b1517.
BAE76457; BAE76457; BAE76457.

GeneID

12932356.
946071.

KEGG

ecj:Y75_p1492.
eco:b1517.

PATRIC

32118330. VBIEscCol129921_1585.

Organism-specific databases

EchoBASE

EB3571.

EcoGene

EG13810. lsrF.

Phylogenomic databases

eggNOG

COG1830.

HOGENOM

HOG000224792.

K08321.

OMA

CEYWGMP.

ProtClustDB

PRK08227.

Enzyme and pathway databases

BioCyc

EcoCyc:G6804-MONOMER.
ECOL316407:JW1510-MONOMER.

Gene expression databases

Genevestigator

P76143.

Family and domain databases

Gene3D

3.20.20.70. 1 hit.

InterPro

IPR013785. Aldolase_TIM.
IPR002915. DeoC/FbaB/lacD_aldolase.
[Graphical view]

Pfam

PF01791. DeoC. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

EvolutionaryTrace

P76143.

Entry information

Entry name

LSRF_ECOLI

Accession

Primary (citable) accession number: P76143
Secondary accession number(s): Q2MB99

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	February 1, 1997
Last modified:	May 1, 2013
This is version 87 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program

Relevant documents

Escherichia coli

Escherichia coli (strain K12): entries and cross-references to EcoGene

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Sites

Secondary structure

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents