P76143 (LSRF_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Uncharacterized aldolase lsrF
EC=4.1.2.-

Gene names

Name:	lsrF
Synonyms:	yneB
Ordered Locus Names:	b1517, JW1510

Organism

Escherichia coli (strain K12)

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	291 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Could be involved in the degradation of phospho-AI-2, thereby terminating induction of the lsr operon and closing the AI-2 signaling cycle By similarity.
Subcellular location	Cytoplasm Potential.
Induction	In the absence of AI-2, repressed by lsrR. Induced by AI-2, via release of the lsrR repressor. In the absence of glucose, induced by cAMP-CRP by direct binding to the upstream region of the lsr promoter. Ref.3 Ref.4
Sequence similarities	Belongs to the DeoC/FbaB aldolase family.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	Schiff base
Molecular function	Lyase
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	lyase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 291

291

Uncharacterized aldolase lsrF

PRO_0000138945

Sites

Active site

203

1

Schiff-base intermediate with substrate By similarity

Secondary structure

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291

Helix Strand Turn

Details...

Helix

37 – 43

7

Beta strand

53 – 55

3

Helix

71 – 74

4

Helix

76 – 81

6

Beta strand

83 – 87

5

Helix

92 – 95

4

Helix

98 – 100

3

Beta strand

104 – 107

4

Helix

127 – 132

6

Beta strand

136 – 141

6

Helix

149 – 161

13

Beta strand

170 – 173

4

Helix

184 – 196

13

Beta strand

201 – 205

5

Helix

212 – 215

4

Beta strand

222 – 225

4

Helix

232 – 244

13

Turn

255 – 258

4

Beta strand

259 – 261

3

Helix

262 – 275

14

Helix

279 – 287

9

Sequences

	Sequence		Length	Mass (Da)
	P76143 [UniParc]. Last modified February 1, 1997. Version 1. Checksum: D5CC245A5C709F1C	FASTA	291	31,893
	10 20 30 40 50 60 MADLDDIKDG KDFRTDQPQK NIPFTLKGCG ALDWGMQSRL SRIFNPKTGK TVMLAFDHGY 70 80 90 100 110 120 FQGPTTGLER IDINIAPLFE HADVLMCTRG ILRSVVPPAT NRPVVLRASG ANSILAELSN 130 140 150 160 170 180 EAVALSMDDA VRLNSCAVAA QVYIGSEYEH QSIKNIIQLV DAGMKVGMPT MAVTGVGKDM 190 200 210 220 230 240 VRDQRYFSLA TRIAAEMGAQ IIKTYYVEKG FERIVAGCPV PIVIAGGKKL PEREALEMCW 250 260 270 280 290 QAIDQGASGV DMGRNIFQSD HPVAMMKAVQ AVVHHNETAD RAYELYLSEK Q

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[2]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed: 16738553] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[3]	"Regulation of uptake and processing of the quorum-sensing autoinducer AI-2 in Escherichia coli." Xavier K.B., Bassler B.L. J. Bacteriol. 187:238-248(2005) [PubMed: 15601708] [Abstract] Cited for: INDUCTION. Strain: K12 / MG1655 / ATCC 47076.
[4]	"Cyclic AMP (cAMP) and cAMP receptor protein influence both synthesis and uptake of extracellular autoinducer 2 in Escherichia coli." Wang L., Hashimoto Y., Tsao C.-Y., Valdes J.J., Bentley W.E. J. Bacteriol. 187:2066-2076(2005) [PubMed: 15743955] [Abstract] Cited for: INDUCTION. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

U00096 Genomic DNA. Translation: AAC74590.1.
AP009048 Genomic DNA. Translation: BAE76457.1.

PIR

H64905.

RefSeq

NP_416034.1. NC_000913.2.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
3GKF	X-ray	2.90	A/B/C/D/E/F/G/H/I/J/K/L/M/N/O/P/Q/R/S/T	1-291	[»]
3GLC	X-ray	2.50	A/B/C/D/E/F/G/H/I/J/K/L/M/N/O/P/Q/R/S/T	1-291	[»]
3GND	X-ray	2.90	A/B/C/D/E/F/G/H/I/J/K/L/M/N/O/P/Q/R/S/T	1-291	[»]

ProteinModelPortal

P76143.

SMR

P76143. Positions 10-290.

ModBase

Search...

Protein-protein interaction databases

DIP

DIP-12754N.

IntAct

P76143. 3 interactions.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

EnsemblBacteria

EBESCT00000002802; EBESCP00000002802; EBESCG00000002288.
EBESCT00000016611; EBESCP00000015902; EBESCG00000015670.

GeneID

946071.

GenomeReviews

Gene locus JW1510 in contig AP009048_GR.
Gene locus b1517 in contig U00096_GR.

KEGG

ecj:JW1510.
eco:b1517.

PATRIC

32118330. VBIEscCol129921_1585.

Organism-specific databases

EchoBASE

EB3571.

EcoGene

EG13810. lsrF.

Phylogenomic databases

eggNOG

COG1830.

GeneTree

EBGT00050000010813.

HOGENOM

HBG553580.

OMA

GTRYGMP.

PhylomeDB

P76143.

ProtClustDB

PRK08227.

Enzyme and pathway databases

BioCyc

EcoCyc:G6804-MONOMER.

Gene expression databases

Genevestigator

P76143.

Family and domain databases

InterPro

IPR013785. Aldolase_TIM.
IPR002915. DeoC/AroFGH_arch.
[Graphical view]

Gene3D

G3DSA:3.20.20.70. Aldolase_TIM. 1 hit.

KO

K08321.

Pfam

PF01791. DeoC. 1 hit.
[Graphical view]

ProtoNet

Search...

Entry information

Entry name

LSRF_ECOLI

Accession

Primary (citable) accession number: P76143
Secondary accession number(s): Q2MB99

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	February 1, 1997
Last modified:	January 25, 2012
This is version 76 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program