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P76143 (LSRF_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 93. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Uncharacterized aldolase LsrF

EC=4.1.2.-
Gene names
Name:lsrF
Synonyms:yneB
Ordered Locus Names:b1517, JW1510
OrganismEscherichia coli (strain K12) [Reference proteome] [HAMAP]
Taxonomic identifier83333 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia

Protein attributes

Sequence length291 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Could be involved in the degradation of phospho-AI-2, thereby terminating induction of the lsr operon and closing the AI-2 signaling cycle By similarity.

Subcellular location

Cytoplasm Potential.

Induction

In the absence of AI-2, repressed by LsrR. Induced by AI-2, via release of the LsrR repressor. In the absence of glucose, induced by cAMP-CRP by direct binding to the upstream region of the lsr promoter. Ref.3 Ref.4

Sequence similarities

Belongs to the DeoC/FbaB aldolase family.

Ontologies

Keywords
   Cellular componentCytoplasm
   LigandSchiff base
   Molecular functionLyase
   Technical term3D-structure
Complete proteome
Reference proteome
Gene Ontology (GO)
   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionlyase activity

Inferred from electronic annotation. Source: UniProtKB-KW

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 291291Uncharacterized aldolase LsrF
PRO_0000138945

Sites

Active site2031Schiff-base intermediate with substrate By similarity

Secondary structure

................................................... 291
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P76143 [UniParc].

Last modified February 1, 1997. Version 1.
Checksum: D5CC245A5C709F1C

FASTA29131,893
        10         20         30         40         50         60 
MADLDDIKDG KDFRTDQPQK NIPFTLKGCG ALDWGMQSRL SRIFNPKTGK TVMLAFDHGY 

        70         80         90        100        110        120 
FQGPTTGLER IDINIAPLFE HADVLMCTRG ILRSVVPPAT NRPVVLRASG ANSILAELSN 

       130        140        150        160        170        180 
EAVALSMDDA VRLNSCAVAA QVYIGSEYEH QSIKNIIQLV DAGMKVGMPT MAVTGVGKDM 

       190        200        210        220        230        240 
VRDQRYFSLA TRIAAEMGAQ IIKTYYVEKG FERIVAGCPV PIVIAGGKKL PEREALEMCW 

       250        260        270        280        290 
QAIDQGASGV DMGRNIFQSD HPVAMMKAVQ AVVHHNETAD RAYELYLSEK Q 

« Hide

References

« Hide 'large scale' references
[1]"The complete genome sequence of Escherichia coli K-12."
Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y.
Science 277:1453-1462(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / MG1655 / ATCC 47076.
[2]"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[3]"Regulation of uptake and processing of the quorum-sensing autoinducer AI-2 in Escherichia coli."
Xavier K.B., Bassler B.L.
J. Bacteriol. 187:238-248(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: INDUCTION.
Strain: K12 / MG1655 / ATCC 47076.
[4]"Cyclic AMP (cAMP) and cAMP receptor protein influence both synthesis and uptake of extracellular autoinducer 2 in Escherichia coli."
Wang L., Hashimoto Y., Tsao C.-Y., Valdes J.J., Bentley W.E.
J. Bacteriol. 187:2066-2076(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: INDUCTION.
Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U00096 Genomic DNA. Translation: AAC74590.1.
AP009048 Genomic DNA. Translation: BAE76457.1.
PIRH64905.
RefSeqNP_416034.1. NC_000913.3.
YP_489780.1. NC_007779.1.

3D structure databases

PDBe
RCSB-PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
3GKFX-ray2.90A/B/C/D/E/F/G/H/I/J/K/L/M/N/O/P/Q/R/S/T1-291[»]
3GLCX-ray2.50A/B/C/D/E/F/G/H/I/J/K/L/M/N/O/P/Q/R/S/T1-291[»]
3GNDX-ray2.90A/B/C/D/E/F/G/H/I/J/K/L/M/N/O/P/Q/R/S/T1-291[»]
ProteinModelPortalP76143.
SMRP76143. Positions 10-290.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

DIPDIP-12754N.
IntActP76143. 5 interactions.
STRING511145.b1517.

Proteomic databases

PaxDbP76143.
PRIDEP76143.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAC74590; AAC74590; b1517.
BAE76457; BAE76457; BAE76457.
GeneID12932356.
946071.
KEGGecj:Y75_p1492.
eco:b1517.
PATRIC32118330. VBIEscCol129921_1585.

Organism-specific databases

EchoBASEEB3571.
EcoGeneEG13810. lsrF.

Phylogenomic databases

eggNOGCOG1830.
HOGENOMHOG000224792.
KOK08321.
OMAGTRYGMP.
OrthoDBEOG6Z6FRM.
PhylomeDBP76143.

Enzyme and pathway databases

BioCycEcoCyc:G6804-MONOMER.
ECOL316407:JW1510-MONOMER.

Gene expression databases

GenevestigatorP76143.

Family and domain databases

Gene3D3.20.20.70. 1 hit.
InterProIPR013785. Aldolase_TIM.
IPR002915. DeoC/FbaB/lacD_aldolase.
[Graphical view]
PfamPF01791. DeoC. 1 hit.
[Graphical view]
PIRSFPIRSF038992. Aldolase_Ia. 1 hit.
ProtoNetSearch...

Other

EvolutionaryTraceP76143.
PROP76143.

Entry information

Entry nameLSRF_ECOLI
AccessionPrimary (citable) accession number: P76143
Secondary accession number(s): Q2MB99
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: February 1, 1997
Last modified: May 14, 2014
This is version 93 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

Escherichia coli

Escherichia coli (strain K12): entries and cross-references to EcoGene