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P76143

- LSRF_ECOLI

UniProt

P76143 - LSRF_ECOLI

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Protein

Uncharacterized aldolase LsrF

Gene
lsrF, yneB, b1517, JW1510
Organism
Escherichia coli (strain K12)
Status
Reviewed - Annotation score: 2 out of 5 - Experimental evidence at protein leveli

Functioni

Could be involved in the degradation of phospho-AI-2, thereby terminating induction of the lsr operon and closing the AI-2 signaling cycle By similarity.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei203 – 2031Schiff-base intermediate with substrate By similarity

GO - Molecular functioni

  1. lyase activity Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Lyase

Keywords - Ligandi

Schiff base

Enzyme and pathway databases

BioCyciEcoCyc:G6804-MONOMER.
ECOL316407:JW1510-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Uncharacterized aldolase LsrF (EC:4.1.2.-)
Gene namesi
Name:lsrF
Synonyms:yneB
Ordered Locus Names:b1517, JW1510
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
ProteomesiUP000000318: Chromosome, UP000000625: Chromosome

Organism-specific databases

EcoGeneiEG13810. lsrF.

Subcellular locationi

Cytoplasm Reviewed prediction

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 291291Uncharacterized aldolase LsrFPRO_0000138945Add
BLAST

Proteomic databases

PaxDbiP76143.
PRIDEiP76143.

Expressioni

Inductioni

In the absence of AI-2, repressed by LsrR. Induced by AI-2, via release of the LsrR repressor. In the absence of glucose, induced by cAMP-CRP by direct binding to the upstream region of the lsr promoter.2 Publications

Gene expression databases

GenevestigatoriP76143.

Interactioni

Protein-protein interaction databases

DIPiDIP-12754N.
IntActiP76143. 5 interactions.
STRINGi511145.b1517.

Structurei

Secondary structure

1
291
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi34 – 4310
Turni46 – 483
Beta strandi51 – 555
Helixi59 – 624
Helixi71 – 744
Helixi76 – 816
Beta strandi83 – 875
Helixi89 – 957
Helixi98 – 1003
Beta strandi104 – 1074
Beta strandi109 – 1113
Helixi127 – 1326
Beta strandi136 – 1427
Helixi149 – 16416
Turni165 – 1673
Beta strandi170 – 1745
Helixi184 – 19613
Beta strandi200 – 2056
Turni208 – 2103
Helixi211 – 2166
Beta strandi222 – 2254
Helixi232 – 24413
Beta strandi248 – 2536
Helixi254 – 2574
Beta strandi259 – 2613
Helixi262 – 27514
Helixi279 – 2879

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3GKFX-ray2.90A/B/C/D/E/F/G/H/I/J/K/L/M/N/O/P/Q/R/S/T1-291[»]
3GLCX-ray2.50A/B/C/D/E/F/G/H/I/J/K/L/M/N/O/P/Q/R/S/T1-291[»]
3GNDX-ray2.90A/B/C/D/E/F/G/H/I/J/K/L/M/N/O/P/Q/R/S/T1-291[»]
ProteinModelPortaliP76143.
SMRiP76143. Positions 10-290.

Miscellaneous databases

EvolutionaryTraceiP76143.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG1830.
HOGENOMiHOG000224792.
KOiK08321.
OMAiGTRYGMP.
OrthoDBiEOG6Z6FRM.
PhylomeDBiP76143.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
InterProiIPR013785. Aldolase_TIM.
IPR002915. DeoC/FbaB/lacD_aldolase.
[Graphical view]
PfamiPF01791. DeoC. 1 hit.
[Graphical view]
PIRSFiPIRSF038992. Aldolase_Ia. 1 hit.

Sequencei

Sequence statusi: Complete.

P76143-1 [UniParc]FASTAAdd to Basket

« Hide

MADLDDIKDG KDFRTDQPQK NIPFTLKGCG ALDWGMQSRL SRIFNPKTGK    50
TVMLAFDHGY FQGPTTGLER IDINIAPLFE HADVLMCTRG ILRSVVPPAT 100
NRPVVLRASG ANSILAELSN EAVALSMDDA VRLNSCAVAA QVYIGSEYEH 150
QSIKNIIQLV DAGMKVGMPT MAVTGVGKDM VRDQRYFSLA TRIAAEMGAQ 200
IIKTYYVEKG FERIVAGCPV PIVIAGGKKL PEREALEMCW QAIDQGASGV 250
DMGRNIFQSD HPVAMMKAVQ AVVHHNETAD RAYELYLSEK Q 291
Length:291
Mass (Da):31,893
Last modified:February 1, 1997 - v1
Checksum:iD5CC245A5C709F1C
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U00096 Genomic DNA. Translation: AAC74590.1.
AP009048 Genomic DNA. Translation: BAE76457.1.
PIRiH64905.
RefSeqiNP_416034.1. NC_000913.3.
YP_489780.1. NC_007779.1.

Genome annotation databases

EnsemblBacteriaiAAC74590; AAC74590; b1517.
BAE76457; BAE76457; BAE76457.
GeneIDi12932356.
946071.
KEGGiecj:Y75_p1492.
eco:b1517.
PATRICi32118330. VBIEscCol129921_1585.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U00096 Genomic DNA. Translation: AAC74590.1 .
AP009048 Genomic DNA. Translation: BAE76457.1 .
PIRi H64905.
RefSeqi NP_416034.1. NC_000913.3.
YP_489780.1. NC_007779.1.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
3GKF X-ray 2.90 A/B/C/D/E/F/G/H/I/J/K/L/M/N/O/P/Q/R/S/T 1-291 [» ]
3GLC X-ray 2.50 A/B/C/D/E/F/G/H/I/J/K/L/M/N/O/P/Q/R/S/T 1-291 [» ]
3GND X-ray 2.90 A/B/C/D/E/F/G/H/I/J/K/L/M/N/O/P/Q/R/S/T 1-291 [» ]
ProteinModelPortali P76143.
SMRi P76143. Positions 10-290.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

DIPi DIP-12754N.
IntActi P76143. 5 interactions.
STRINGi 511145.b1517.

Proteomic databases

PaxDbi P76143.
PRIDEi P76143.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAC74590 ; AAC74590 ; b1517 .
BAE76457 ; BAE76457 ; BAE76457 .
GeneIDi 12932356.
946071.
KEGGi ecj:Y75_p1492.
eco:b1517.
PATRICi 32118330. VBIEscCol129921_1585.

Organism-specific databases

EchoBASEi EB3571.
EcoGenei EG13810. lsrF.

Phylogenomic databases

eggNOGi COG1830.
HOGENOMi HOG000224792.
KOi K08321.
OMAi GTRYGMP.
OrthoDBi EOG6Z6FRM.
PhylomeDBi P76143.

Enzyme and pathway databases

BioCyci EcoCyc:G6804-MONOMER.
ECOL316407:JW1510-MONOMER.

Miscellaneous databases

EvolutionaryTracei P76143.
PROi P76143.

Gene expression databases

Genevestigatori P76143.

Family and domain databases

Gene3Di 3.20.20.70. 1 hit.
InterProi IPR013785. Aldolase_TIM.
IPR002915. DeoC/FbaB/lacD_aldolase.
[Graphical view ]
Pfami PF01791. DeoC. 1 hit.
[Graphical view ]
PIRSFi PIRSF038992. Aldolase_Ia. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  2. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
    Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
    Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  3. "Regulation of uptake and processing of the quorum-sensing autoinducer AI-2 in Escherichia coli."
    Xavier K.B., Bassler B.L.
    J. Bacteriol. 187:238-248(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: INDUCTION.
    Strain: K12 / MG1655 / ATCC 47076.
  4. "Cyclic AMP (cAMP) and cAMP receptor protein influence both synthesis and uptake of extracellular autoinducer 2 in Escherichia coli."
    Wang L., Hashimoto Y., Tsao C.-Y., Valdes J.J., Bentley W.E.
    J. Bacteriol. 187:2066-2076(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: INDUCTION.
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.

Entry informationi

Entry nameiLSRF_ECOLI
AccessioniPrimary (citable) accession number: P76143
Secondary accession number(s): Q2MB99
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: February 1, 1997
Last modified: May 14, 2014
This is version 93 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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