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Protein

Autoinducer 2-binding protein LsrB

Gene

lsrB

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Part of the ABC transporter complex LsrABCD involved in autoinducer 2 (AI-2) import. Binds AI-2 and delivers it to the LsrC and LsrD permeases (Probable).1 Publication

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Enzyme and pathway databases

BioCyciEcoCyc:YNEA-MONOMER.
ECOL316407:JW1509-MONOMER.
MetaCyc:YNEA-MONOMER.

Protein family/group databases

TCDBi3.A.1.2.8. the atp-binding cassette (abc) superfamily.

Names & Taxonomyi

Protein namesi
Recommended name:
Autoinducer 2-binding protein LsrB
Short name:
AI-2-binding protein LsrB
Gene namesi
Name:lsrB
Synonyms:yneA
Ordered Locus Names:b1516, JW1509
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG13809. lsrB.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Periplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2626Sequence analysisAdd
BLAST
Chaini27 – 340314Autoinducer 2-binding protein LsrBPRO_0000013777Add
BLAST

Proteomic databases

PaxDbiP76142.
PRIDEiP76142.

Expressioni

Inductioni

In the absence of AI-2, repressed by LsrR. Induced by AI-2, via release of the LsrR repressor. In the absence of glucose, induced by cAMP-CRP by direct binding to the upstream region of the lsr promoter.2 Publications

Interactioni

Subunit structurei

The complex is composed of two ATP-binding proteins (LsrA), two transmembrane proteins (LsrC and LsrD) and a solute-binding protein (LsrB).Curated

Protein-protein interaction databases

BioGridi4261349. 9 interactions.
IntActiP76142. 1 interaction.
STRINGi511145.b1516.

Structurei

3D structure databases

ProteinModelPortaliP76142.
SMRiP76142. Positions 26-340.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiENOG4105D4B. Bacteria.
ENOG410XPHQ. LUCA.
HOGENOMiHOG000092666.
InParanoidiP76142.
KOiK10555.
OMAiKKKYPNW.
OrthoDBiEOG6PP9MR.
PhylomeDBiP76142.

Family and domain databases

InterProiIPR030159. LsrB/RhaS.
IPR028082. Peripla_BP_I.
IPR025997. SBP_2_dom.
[Graphical view]
PANTHERiPTHR32196:SF1. PTHR32196:SF1. 1 hit.
PfamiPF13407. Peripla_BP_4. 1 hit.
[Graphical view]
SUPFAMiSSF53822. SSF53822. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P76142-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTLHRFKKIA LLSALGIAAI SMNVQAAERI AFIPKLVGVG FFTSGGNGAQ
60 70 80 90 100
QAGKELGVDV TYDGPTEPSV SGQVQLINNF VNQGYNAIIV SAVSPDGLCP
110 120 130 140 150
ALKRAMQRGV RVLTWDSDTK PECRSYYINQ GTPAQLGGML VDMAARQVNK
160 170 180 190 200
DKAKVAFFYS SPTVTDQNQW VKEAKAKIAK EHPGWEIVTT QFGYNDATKS
210 220 230 240 250
LQTAEGILKA YSDLDAIIAP DANALPAAAQ AAENLKNDKV AIVGFSTPNV
260 270 280 290 300
MRPYVERGTV KEFGLWDVVQ QGKISVYVAD ALLKKGSMKT GDKLDIKGVG
310 320 330 340
QVEVSPNSVQ GYDYEADGNG IVLLPERVIF NKENIGKYDF
Length:340
Mass (Da):36,685
Last modified:February 1, 1997 - v1
Checksum:iD11AFAD3FB62A3CE
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00096 Genomic DNA. Translation: AAC74589.1.
AP009048 Genomic DNA. Translation: BAE76456.1.
PIRiG64905.
RefSeqiNP_416033.1. NC_000913.3.
WP_000172465.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC74589; AAC74589; b1516.
BAE76456; BAE76456; BAE76456.
GeneIDi945418.
KEGGiecj:JW1509.
eco:b1516.
PATRICi32118328. VBIEscCol129921_1584.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00096 Genomic DNA. Translation: AAC74589.1.
AP009048 Genomic DNA. Translation: BAE76456.1.
PIRiG64905.
RefSeqiNP_416033.1. NC_000913.3.
WP_000172465.1. NZ_LN832404.1.

3D structure databases

ProteinModelPortaliP76142.
SMRiP76142. Positions 26-340.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4261349. 9 interactions.
IntActiP76142. 1 interaction.
STRINGi511145.b1516.

Protein family/group databases

TCDBi3.A.1.2.8. the atp-binding cassette (abc) superfamily.

Proteomic databases

PaxDbiP76142.
PRIDEiP76142.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC74589; AAC74589; b1516.
BAE76456; BAE76456; BAE76456.
GeneIDi945418.
KEGGiecj:JW1509.
eco:b1516.
PATRICi32118328. VBIEscCol129921_1584.

Organism-specific databases

EchoBASEiEB3570.
EcoGeneiEG13809. lsrB.

Phylogenomic databases

eggNOGiENOG4105D4B. Bacteria.
ENOG410XPHQ. LUCA.
HOGENOMiHOG000092666.
InParanoidiP76142.
KOiK10555.
OMAiKKKYPNW.
OrthoDBiEOG6PP9MR.
PhylomeDBiP76142.

Enzyme and pathway databases

BioCyciEcoCyc:YNEA-MONOMER.
ECOL316407:JW1509-MONOMER.
MetaCyc:YNEA-MONOMER.

Miscellaneous databases

PROiP76142.

Family and domain databases

InterProiIPR030159. LsrB/RhaS.
IPR028082. Peripla_BP_I.
IPR025997. SBP_2_dom.
[Graphical view]
PANTHERiPTHR32196:SF1. PTHR32196:SF1. 1 hit.
PfamiPF13407. Peripla_BP_4. 1 hit.
[Graphical view]
SUPFAMiSSF53822. SSF53822. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  2. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
    Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
    Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  3. "Regulation of uptake and processing of the quorum-sensing autoinducer AI-2 in Escherichia coli."
    Xavier K.B., Bassler B.L.
    J. Bacteriol. 187:238-248(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION IN AI-2 IMPORT, INDUCTION.
    Strain: K12 / MG1655 / ATCC 47076.
  4. "Cyclic AMP (cAMP) and cAMP receptor protein influence both synthesis and uptake of extracellular autoinducer 2 in Escherichia coli."
    Wang L., Hashimoto Y., Tsao C.-Y., Valdes J.J., Bentley W.E.
    J. Bacteriol. 187:2066-2076(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: INDUCTION.
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.

Entry informationi

Entry nameiLSRB_ECOLI
AccessioniPrimary (citable) accession number: P76142
Secondary accession number(s): Q2MBA0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 27, 2002
Last sequence update: February 1, 1997
Last modified: February 17, 2016
This is version 100 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.