Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Two-component-system connector protein SafA

Gene

safA

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Connects the signal transduction between the two-component systems EvgS/EvgA and PhoQ/PhoP, by directly interacting with PhoQ and thus activating the PhoQ/PhoP system, in response to acid stress conditions.1 Publication

Keywords - Biological processi

Stress response

Enzyme and pathway databases

BioCyciEcoCyc:MONOMER0-2841.

Names & Taxonomyi

Protein namesi
Recommended name:
Two-component-system connector protein SafA
Alternative name(s):
Regulatory protein b1500
Sensor associating factor A
Gene namesi
Name:safA
Synonyms:yneN
Ordered Locus Names:b1500, JW1494.1
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG14466. safA.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 1818Cytoplasmic1 PublicationAdd
BLAST
Transmembranei19 – 3921Helical; Signal-anchor for type II membrane proteinSequence analysisAdd
BLAST
Topological domaini40 – 6526Periplasmic1 PublicationAdd
BLAST

GO - Cellular componenti

  • integral component of plasma membrane Source: EcoCyc
Complete GO annotation...

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 6565Two-component-system connector protein SafAPRO_0000223715Add
BLAST

Proteomic databases

PaxDbiP76136.
PRIDEiP76136.

Expressioni

Inductioni

By acid stress, via the EvgS/EvgA system.2 Publications

Interactioni

Subunit structurei

Interacts with PhoQ.1 Publication

Protein-protein interaction databases

DIPiDIP-46181N.
STRINGi511145.b1500.

Structurei

3D structure databases

ProteinModelPortaliP76136.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the SafA family.Curated

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

HOGENOMiHOG000059395.
OMAiNYKGIMS.
OrthoDBiEOG6F81WQ.

Family and domain databases

InterProiIPR031411. SafA.
[Graphical view]
PfamiPF17073. SafA. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P76136-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MHATTVKNKI TQRDNYKEIM SAIVVVLLLT LTLIAIFSAI DQLSISEMGR
60
IARDLTHFII NSLQG
Length:65
Mass (Da):7,272
Last modified:February 1, 1997 - v1
Checksum:i10ECD1B9EE15463E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00096 Genomic DNA. Translation: AAC74573.1.
AP009048 Genomic DNA. No translation available.
PIRiG64903.
RefSeqiNP_416017.1. NC_000913.3.
WP_000543384.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC74573; AAC74573; b1500.
GeneIDi946061.
KEGGieco:b1500.
PATRICi32118294. VBIEscCol129921_1567.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00096 Genomic DNA. Translation: AAC74573.1.
AP009048 Genomic DNA. No translation available.
PIRiG64903.
RefSeqiNP_416017.1. NC_000913.3.
WP_000543384.1. NZ_LN832404.1.

3D structure databases

ProteinModelPortaliP76136.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-46181N.
STRINGi511145.b1500.

Proteomic databases

PaxDbiP76136.
PRIDEiP76136.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC74573; AAC74573; b1500.
GeneIDi946061.
KEGGieco:b1500.
PATRICi32118294. VBIEscCol129921_1567.

Organism-specific databases

EcoGeneiEG14466. safA.

Phylogenomic databases

HOGENOMiHOG000059395.
OMAiNYKGIMS.
OrthoDBiEOG6F81WQ.

Enzyme and pathway databases

BioCyciEcoCyc:MONOMER0-2841.

Miscellaneous databases

PROiP76136.

Family and domain databases

InterProiIPR031411. SafA.
[Graphical view]
PfamiPF17073. SafA. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  2. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
    Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
    Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  3. "Regulatory network of acid resistance genes in Escherichia coli."
    Masuda N., Church G.M.
    Mol. Microbiol. 48:699-712(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: INDUCTION BY ACID STRESS.
    Strain: K12 / MG1655 / ATCC 47076.
  4. "B1500, a small membrane protein, connects the two-component systems EvgS/EvgA and PhoQ/PhoP in Escherichia coli."
    Eguchi Y., Itou J., Yamane M., Demizu R., Yamato F., Okada A., Mori H., Kato A., Utsumi R.
    Proc. Natl. Acad. Sci. U.S.A. 104:18712-18717(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION IN RESPONSE TO ACID STRESS, SUBCELLULAR LOCATION, TOPOLOGY, INTERACTION WITH PHOQ.
    Strain: K12 / MG1655 / ATCC 47076.
  5. "Molecular mechanism of transcriptional cascade initiated by the EvgS/EvgA system in Escherichia coli K-12."
    Itou J., Eguchi Y., Utsumi R.
    Biosci. Biotechnol. Biochem. 73:870-878(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: INDUCTION, GENE NAME.
    Strain: K12 / MG1655 / ATCC 47076.

Entry informationi

Entry nameiSAFA_ECOLI
AccessioniPrimary (citable) accession number: P76136
Secondary accession number(s): B2D6K4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 21, 2006
Last sequence update: February 1, 1997
Last modified: November 11, 2015
This is version 79 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.