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Protein

HTH-type transcriptional regulator McbR

Gene

mcbR

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Important for biofilm formation. Represses expression of McbA by binding to its promoter region, which prevents colanic acid overproduction and mucoidy.1 Publication

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
DNA bindingi37 – 5620H-T-H motifPROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

  • DNA binding Source: UniProtKB-KW
  • sequence-specific DNA binding transcription factor activity Source: EcoCyc

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Repressor

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding

Enzyme and pathway databases

BioCyciEcoCyc:G6761-MONOMER.
ECOL316407:JW1445-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
HTH-type transcriptional regulator McbR
Gene namesi
Name:mcbR
Synonyms:yncC
Ordered Locus Names:b1450, JW1445
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
ProteomesiUP000000318 Componenti: Chromosome UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG13773. mcbR.

Pathology & Biotechi

Disruption phenotypei

Disruption of this gene induces 30 genes and represses 10 genes more than fourfold. It decreases biofilm formation, increases extracellular indole, leads to colanic acid overproduction and elicits mucoidy.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 221221HTH-type transcriptional regulator McbRPRO_0000050686Add
BLAST

Proteomic databases

PaxDbiP76114.
PRIDEiP76114.

Expressioni

Inductioni

Induced by LsrR, LsrK, MqsR and TqsA. Repressed by LuxS and YgiV.1 Publication

Interactioni

Protein-protein interaction databases

DIPiDIP-12749N.
IntActiP76114. 11 interactions.
MINTiMINT-1303162.
STRINGi511145.b1450.

Structurei

3D structure databases

ProteinModelPortaliP76114.
SMRiP76114. Positions 10-220.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini10 – 7768HTH gntR-typePROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 HTH gntR-type DNA-binding domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiCOG1802.
HOGENOMiHOG000273993.
InParanoidiP76114.
KOiK13654.
OMAiERIENYH.
OrthoDBiEOG6JX7PH.
PhylomeDBiP76114.

Family and domain databases

Gene3Di1.10.10.10. 1 hit.
1.20.120.530. 1 hit.
InterProiIPR011711. GntR_C.
IPR008920. TF_FadR/GntR_C.
IPR000524. Tscrpt_reg_HTH_GntR.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PfamiPF07729. FCD. 1 hit.
PF00392. GntR. 1 hit.
[Graphical view]
SMARTiSM00895. FCD. 1 hit.
SM00345. HTH_GNTR. 1 hit.
[Graphical view]
SUPFAMiSSF48008. SSF48008. 1 hit.
PROSITEiPS50949. HTH_GNTR. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P76114-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPGTGKMKHV SLTLQVENDL KHQLSIGALK PGARLITKNL AEQLGMSITP
60 70 80 90 100
VREALLRLVS VNALSVAPAQ AFTVPEVGKR QLDEINRIRY ELELMAVALA
110 120 130 140 150
VENLTPQDLA ELQELLEKLQ QAQEKGDMEQ IINVNRLFRL AIYHRSNMPI
160 170 180 190 200
LCEMIEQLWV RMGPGLHYLY EAINPAELRE HIENYHLLLA ALKAKDKEGC
210 220
RHCLAEIMQQ NIAILYQQYN R
Length:221
Mass (Da):25,151
Last modified:July 15, 1999 - v2
Checksum:i7A589BCFAE20D75C
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00096 Genomic DNA. Translation: AAC74532.2.
AP009048 Genomic DNA. Translation: BAA15082.1.
PIRiE64897.
RefSeqiNP_415967.2. NC_000913.3.

Genome annotation databases

EnsemblBacteriaiAAC74532; AAC74532; b1450.
BAA15082; BAA15082; BAA15082.
GeneIDi946014.
KEGGiecj:Y75_p1426.
eco:b1450.
PATRICi32118192. VBIEscCol129921_1516.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00096 Genomic DNA. Translation: AAC74532.2.
AP009048 Genomic DNA. Translation: BAA15082.1.
PIRiE64897.
RefSeqiNP_415967.2. NC_000913.3.

3D structure databases

ProteinModelPortaliP76114.
SMRiP76114. Positions 10-220.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-12749N.
IntActiP76114. 11 interactions.
MINTiMINT-1303162.
STRINGi511145.b1450.

Proteomic databases

PaxDbiP76114.
PRIDEiP76114.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC74532; AAC74532; b1450.
BAA15082; BAA15082; BAA15082.
GeneIDi946014.
KEGGiecj:Y75_p1426.
eco:b1450.
PATRICi32118192. VBIEscCol129921_1516.

Organism-specific databases

EchoBASEiEB3535.
EcoGeneiEG13773. mcbR.

Phylogenomic databases

eggNOGiCOG1802.
HOGENOMiHOG000273993.
InParanoidiP76114.
KOiK13654.
OMAiERIENYH.
OrthoDBiEOG6JX7PH.
PhylomeDBiP76114.

Enzyme and pathway databases

BioCyciEcoCyc:G6761-MONOMER.
ECOL316407:JW1445-MONOMER.

Miscellaneous databases

PROiP76114.

Family and domain databases

Gene3Di1.10.10.10. 1 hit.
1.20.120.530. 1 hit.
InterProiIPR011711. GntR_C.
IPR008920. TF_FadR/GntR_C.
IPR000524. Tscrpt_reg_HTH_GntR.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PfamiPF07729. FCD. 1 hit.
PF00392. GntR. 1 hit.
[Graphical view]
SMARTiSM00895. FCD. 1 hit.
SM00345. HTH_GNTR. 1 hit.
[Graphical view]
SUPFAMiSSF48008. SSF48008. 1 hit.
PROSITEiPS50949. HTH_GNTR. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  3. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
    Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
    Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  4. "Escherichia coli transcription factor YncC (McbR) regulates colanic acid and biofilm formation by repressing expression of periplasmic protein YbiM (McbA)."
    Zhang X.-S., Garcia-Contreras R., Wood T.K.
    ISME J. 2:615-631(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION IN BIOFILM FORMATION, INDUCTION, DISRUPTION PHENOTYPE.
    Strain: K12 / BW25113.

Entry informationi

Entry nameiMCBR_ECOLI
AccessioniPrimary (citable) accession number: P76114
Secondary accession number(s): P77347
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1999
Last sequence update: July 15, 1999
Last modified: June 24, 2015
This is version 117 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.