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P76114

- MCBR_ECOLI

UniProt

P76114 - MCBR_ECOLI

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Protein

HTH-type transcriptional regulator McbR

Gene
mcbR, yncC, b1450, JW1445
Organism
Escherichia coli (strain K12)
Status
Reviewed - Annotation score: 3 out of 5 - Experimental evidence at protein leveli

Functioni

Important for biofilm formation. Represses expression of McbA by binding to its promoter region, which prevents colanic acid overproduction and mucoidy.1 Publication

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
DNA bindingi37 – 5620H-T-H motif Reviewed predictionAdd
BLAST

GO - Molecular functioni

  1. DNA binding Source: UniProtKB-KW
  2. sequence-specific DNA binding transcription factor activity Source: EcoCyc

GO - Biological processi

  1. negative regulation of transcription, DNA-templated Source: EcoCyc
  2. transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Repressor

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding

Enzyme and pathway databases

BioCyciEcoCyc:G6761-MONOMER.
ECOL316407:JW1445-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
HTH-type transcriptional regulator McbR
Gene namesi
Name:mcbR
Synonyms:yncC
Ordered Locus Names:b1450, JW1445
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
ProteomesiUP000000318: Chromosome, UP000000625: Chromosome

Organism-specific databases

EcoGeneiEG13773. mcbR.

Pathology & Biotechi

Disruption phenotypei

Disruption of this gene induces 30 genes and represses 10 genes more than fourfold. It decreases biofilm formation, increases extracellular indole, leads to colanic acid overproduction and elicits mucoidy.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 221221HTH-type transcriptional regulator McbRPRO_0000050686Add
BLAST

Proteomic databases

PaxDbiP76114.
PRIDEiP76114.

Expressioni

Inductioni

Induced by LsrR, LsrK, MqsR and TqsA. Repressed by LuxS and YgiV.1 Publication

Gene expression databases

GenevestigatoriP76114.

Interactioni

Protein-protein interaction databases

DIPiDIP-12749N.
IntActiP76114. 11 interactions.
MINTiMINT-1303162.
STRINGi511145.b1450.

Structurei

3D structure databases

ProteinModelPortaliP76114.
SMRiP76114. Positions 4-219.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini10 – 7768HTH gntR-typeAdd
BLAST

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG1802.
HOGENOMiHOG000273993.
KOiK13654.
OMAiERIENYH.
OrthoDBiEOG6JX7PH.
PhylomeDBiP76114.

Family and domain databases

Gene3Di1.10.10.10. 1 hit.
1.20.120.530. 1 hit.
InterProiIPR011711. GntR_C.
IPR008920. TF_FadR/GntR_C.
IPR000524. Tscrpt_reg_HTH_GntR.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PfamiPF07729. FCD. 1 hit.
PF00392. GntR. 1 hit.
[Graphical view]
SMARTiSM00895. FCD. 1 hit.
SM00345. HTH_GNTR. 1 hit.
[Graphical view]
SUPFAMiSSF48008. SSF48008. 1 hit.
PROSITEiPS50949. HTH_GNTR. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P76114-1 [UniParc]FASTAAdd to Basket

« Hide

MPGTGKMKHV SLTLQVENDL KHQLSIGALK PGARLITKNL AEQLGMSITP    50
VREALLRLVS VNALSVAPAQ AFTVPEVGKR QLDEINRIRY ELELMAVALA 100
VENLTPQDLA ELQELLEKLQ QAQEKGDMEQ IINVNRLFRL AIYHRSNMPI 150
LCEMIEQLWV RMGPGLHYLY EAINPAELRE HIENYHLLLA ALKAKDKEGC 200
RHCLAEIMQQ NIAILYQQYN R 221
Length:221
Mass (Da):25,151
Last modified:July 15, 1999 - v2
Checksum:i7A589BCFAE20D75C
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U00096 Genomic DNA. Translation: AAC74532.2.
AP009048 Genomic DNA. Translation: BAA15082.1.
PIRiE64897.
RefSeqiNP_415967.2. NC_000913.3.
YP_489715.1. NC_007779.1.

Genome annotation databases

EnsemblBacteriaiAAC74532; AAC74532; b1450.
BAA15082; BAA15082; BAA15082.
GeneIDi12931202.
946014.
KEGGiecj:Y75_p1426.
eco:b1450.
PATRICi32118192. VBIEscCol129921_1516.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U00096 Genomic DNA. Translation: AAC74532.2 .
AP009048 Genomic DNA. Translation: BAA15082.1 .
PIRi E64897.
RefSeqi NP_415967.2. NC_000913.3.
YP_489715.1. NC_007779.1.

3D structure databases

ProteinModelPortali P76114.
SMRi P76114. Positions 4-219.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

DIPi DIP-12749N.
IntActi P76114. 11 interactions.
MINTi MINT-1303162.
STRINGi 511145.b1450.

Proteomic databases

PaxDbi P76114.
PRIDEi P76114.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAC74532 ; AAC74532 ; b1450 .
BAA15082 ; BAA15082 ; BAA15082 .
GeneIDi 12931202.
946014.
KEGGi ecj:Y75_p1426.
eco:b1450.
PATRICi 32118192. VBIEscCol129921_1516.

Organism-specific databases

EchoBASEi EB3535.
EcoGenei EG13773. mcbR.

Phylogenomic databases

eggNOGi COG1802.
HOGENOMi HOG000273993.
KOi K13654.
OMAi ERIENYH.
OrthoDBi EOG6JX7PH.
PhylomeDBi P76114.

Enzyme and pathway databases

BioCyci EcoCyc:G6761-MONOMER.
ECOL316407:JW1445-MONOMER.

Miscellaneous databases

PROi P76114.

Gene expression databases

Genevestigatori P76114.

Family and domain databases

Gene3Di 1.10.10.10. 1 hit.
1.20.120.530. 1 hit.
InterProi IPR011711. GntR_C.
IPR008920. TF_FadR/GntR_C.
IPR000524. Tscrpt_reg_HTH_GntR.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view ]
Pfami PF07729. FCD. 1 hit.
PF00392. GntR. 1 hit.
[Graphical view ]
SMARTi SM00895. FCD. 1 hit.
SM00345. HTH_GNTR. 1 hit.
[Graphical view ]
SUPFAMi SSF48008. SSF48008. 1 hit.
PROSITEi PS50949. HTH_GNTR. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  3. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
    Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
    Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  4. "Escherichia coli transcription factor YncC (McbR) regulates colanic acid and biofilm formation by repressing expression of periplasmic protein YbiM (McbA)."
    Zhang X.-S., Garcia-Contreras R., Wood T.K.
    ISME J. 2:615-631(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION IN BIOFILM FORMATION, INDUCTION, DISRUPTION PHENOTYPE.
    Strain: K12 / BW25113.

Entry informationi

Entry nameiMCBR_ECOLI
AccessioniPrimary (citable) accession number: P76114
Secondary accession number(s): P77347
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1999
Last sequence update: July 15, 1999
Last modified: May 14, 2014
This is version 112 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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