P76114 (MCBR_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
HTH-type transcriptional regulator McbR

Gene names

Name:	mcbR
Synonyms:	yncC
Ordered Locus Names:	b1450, JW1445

Organism

Escherichia coli (strain K12) [Reference proteome] [HAMAP]

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia › Escherichia coli

Protein attributes

Sequence length	221 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Important for biofilm formation. Represses expression of McbA by binding to its promoter region, which prevents colanic acid overproduction and mucoidy. Ref.4
Induction	Induced by LsrR, LsrK, MqsR and TqsA. Repressed by LuxS and YgiV. Ref.4
Disruption phenotype	Disruption of this gene induces 30 genes and represses 10 genes more than fourfold. It decreases biofilm formation, increases extracellular indole, leads to colanic acid overproduction and elicits mucoidy. Ref.4
Sequence similarities	Contains 1 HTH gntR-type DNA-binding domain.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Ligand	DNA-binding
Molecular function	Repressor
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	negative regulation of transcription, DNA-dependent Inferred from direct assay Ref.4. Source: EcoCyc transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	intracellular Inferred from electronic annotation. Source: InterPro
Molecular_function	DNA binding Inferred from electronic annotation. Source: UniProtKB-KW sequence-specific DNA binding transcription factor activity Inferred from direct assay Ref.4. Source: EcoCyc
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
rplC	P60438	1	EBI-560824,EBI-542200

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 221	221	HTH-type transcriptional regulator McbR		PRO_0000050686
Regions
	Domain	10 – 77	68	HTH gntR-type
	DNA binding	37 – 56	20	H-T-H motif Potential

Sequences

	Sequence		Length	Mass (Da)
	P76114 [UniParc]. Last modified July 15, 1999. Version 2. Checksum: 7A589BCFAE20D75C	FASTA	221	25,151
	10 20 30 40 50 60 MPGTGKMKHV SLTLQVENDL KHQLSIGALK PGARLITKNL AEQLGMSITP VREALLRLVS 70 80 90 100 110 120 VNALSVAPAQ AFTVPEVGKR QLDEINRIRY ELELMAVALA VENLTPQDLA ELQELLEKLQ 130 140 150 160 170 180 QAQEKGDMEQ IINVNRLFRL AIYHRSNMPI LCEMIEQLWV RMGPGLHYLY EAINPAELRE 190 200 210 220 HIENYHLLLA ALKAKDKEGC RHCLAEIMQQ NIAILYQQYN R

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"A 570-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 28.0-40.1 min region on the linkage map." Aiba H., Baba T., Fujita K., Hayashi K., Inada T., Isono K., Itoh T., Kasai H., Kashimoto K., Kimura S., Kitakawa M., Kitagawa M., Makino K., Miki T., Mizobuchi K., Mori H., Mori T., Motomura K. , Nakade S., Nakamura Y., Nashimoto H., Nishio Y., Oshima T., Saito N., Sampei G., Seki Y., Sivasundaram S., Tagami H., Takeda J., Takemoto K., Takeuchi Y., Wada C., Yamamoto Y., Horiuchi T. DNA Res. 3:363-377(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[2]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[3]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[4]	"Escherichia coli transcription factor YncC (McbR) regulates colanic acid and biofilm formation by repressing expression of periplasmic protein YbiM (McbA)." Zhang X.-S., Garcia-Contreras R., Wood T.K. ISME J. 2:615-631(2008) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION IN BIOFILM FORMATION, INDUCTION, DISRUPTION PHENOTYPE. Strain: K12 / BW25113.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U00096 Genomic DNA. Translation: AAC74532.2. AP009048 Genomic DNA. Translation: BAA15082.1.
PIR	E64897.
RefSeq	NP_415967.2. NC_000913.2. YP_489715.1. NC_007779.1.
3D structure databases
ProteinModelPortal	P76114.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-12749N.
IntAct	P76114. 9 interactions.
MINT	MINT-1303162.
STRING	511145.b1450.
Proteomic databases
PaxDb	P76114.
PRIDE	P76114.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAC74532; AAC74532; b1450. BAA15082; BAA15082; BAA15082.
GeneID	12931202. 946014.
KEGG	ecj:Y75_p1426. eco:b1450.
PATRIC	32118192. VBIEscCol129921_1516.
Organism-specific databases
EchoBASE	EB3535.
EcoGene	EG13773. mcbR.
Phylogenomic databases
eggNOG	COG1802.
HOGENOM	HOG000273993.
KO	K13654.
OMA	ERIENYH.
ProtClustDB	PRK11414.
Enzyme and pathway databases
BioCyc	EcoCyc:G6761-MONOMER. ECOL316407:JW1445-MONOMER.
Gene expression databases
Genevestigator	P76114.
Family and domain databases
Gene3D	1.10.10.10. 1 hit.
InterPro	IPR011711. GntR_C. IPR000524. Tscrpt_reg_HTH_GntR. IPR011991. WHTH_DNA-bd_dom. [Graphical view]
Pfam	PF07729. FCD. 1 hit. PF00392. GntR. 1 hit. [Graphical view]
SMART	SM00895. FCD. 1 hit. SM00345. HTH_GNTR. 1 hit. [Graphical view]
PROSITE	PS50949. HTH_GNTR. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MCBR_ECOLI

Accession

Primary (citable) accession number: P76114
Secondary accession number(s): P77347

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1999
Last sequence update:	July 15, 1999
Last modified:	May 1, 2013
This is version 102 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program