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P76114 (MCBR_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 112. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
HTH-type transcriptional regulator McbR
Gene names
Name:mcbR
Synonyms:yncC
Ordered Locus Names:b1450, JW1445
OrganismEscherichia coli (strain K12) [Reference proteome] [HAMAP]
Taxonomic identifier83333 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia

Protein attributes

Sequence length221 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Important for biofilm formation. Represses expression of McbA by binding to its promoter region, which prevents colanic acid overproduction and mucoidy. Ref.4

Induction

Induced by LsrR, LsrK, MqsR and TqsA. Repressed by LuxS and YgiV. Ref.4

Disruption phenotype

Disruption of this gene induces 30 genes and represses 10 genes more than fourfold. It decreases biofilm formation, increases extracellular indole, leads to colanic acid overproduction and elicits mucoidy. Ref.4

Sequence similarities

Contains 1 HTH gntR-type DNA-binding domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 221221HTH-type transcriptional regulator McbR
PRO_0000050686

Regions

Domain10 – 7768HTH gntR-type
DNA binding37 – 5620H-T-H motif Potential

Sequences

Sequence LengthMass (Da)Tools
P76114 [UniParc].

Last modified July 15, 1999. Version 2.
Checksum: 7A589BCFAE20D75C

FASTA22125,151
        10         20         30         40         50         60 
MPGTGKMKHV SLTLQVENDL KHQLSIGALK PGARLITKNL AEQLGMSITP VREALLRLVS 

        70         80         90        100        110        120 
VNALSVAPAQ AFTVPEVGKR QLDEINRIRY ELELMAVALA VENLTPQDLA ELQELLEKLQ 

       130        140        150        160        170        180 
QAQEKGDMEQ IINVNRLFRL AIYHRSNMPI LCEMIEQLWV RMGPGLHYLY EAINPAELRE 

       190        200        210        220 
HIENYHLLLA ALKAKDKEGC RHCLAEIMQQ NIAILYQQYN R 

« Hide

References

« Hide 'large scale' references
[1]"A 570-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 28.0-40.1 min region on the linkage map."
Aiba H., Baba T., Fujita K., Hayashi K., Inada T., Isono K., Itoh T., Kasai H., Kashimoto K., Kimura S., Kitakawa M., Kitagawa M., Makino K., Miki T., Mizobuchi K., Mori H., Mori T., Motomura K. expand/collapse author list , Nakade S., Nakamura Y., Nashimoto H., Nishio Y., Oshima T., Saito N., Sampei G., Seki Y., Sivasundaram S., Tagami H., Takeda J., Takemoto K., Takeuchi Y., Wada C., Yamamoto Y., Horiuchi T.
DNA Res. 3:363-377(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[2]"The complete genome sequence of Escherichia coli K-12."
Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y.
Science 277:1453-1462(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / MG1655 / ATCC 47076.
[3]"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[4]"Escherichia coli transcription factor YncC (McbR) regulates colanic acid and biofilm formation by repressing expression of periplasmic protein YbiM (McbA)."
Zhang X.-S., Garcia-Contreras R., Wood T.K.
ISME J. 2:615-631(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION IN BIOFILM FORMATION, INDUCTION, DISRUPTION PHENOTYPE.
Strain: K12 / BW25113.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U00096 Genomic DNA. Translation: AAC74532.2.
AP009048 Genomic DNA. Translation: BAA15082.1.
PIRE64897.
RefSeqNP_415967.2. NC_000913.3.
YP_489715.1. NC_007779.1.

3D structure databases

ProteinModelPortalP76114.
SMRP76114. Positions 4-219.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

DIPDIP-12749N.
IntActP76114. 11 interactions.
MINTMINT-1303162.
STRING511145.b1450.

Proteomic databases

PaxDbP76114.
PRIDEP76114.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAC74532; AAC74532; b1450.
BAA15082; BAA15082; BAA15082.
GeneID12931202.
946014.
KEGGecj:Y75_p1426.
eco:b1450.
PATRIC32118192. VBIEscCol129921_1516.

Organism-specific databases

EchoBASEEB3535.
EcoGeneEG13773. mcbR.

Phylogenomic databases

eggNOGCOG1802.
HOGENOMHOG000273993.
KOK13654.
OMAERIENYH.
OrthoDBEOG6JX7PH.
PhylomeDBP76114.

Enzyme and pathway databases

BioCycEcoCyc:G6761-MONOMER.
ECOL316407:JW1445-MONOMER.

Gene expression databases

GenevestigatorP76114.

Family and domain databases

Gene3D1.10.10.10. 1 hit.
1.20.120.530. 1 hit.
InterProIPR011711. GntR_C.
IPR008920. TF_FadR/GntR_C.
IPR000524. Tscrpt_reg_HTH_GntR.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PfamPF07729. FCD. 1 hit.
PF00392. GntR. 1 hit.
[Graphical view]
SMARTSM00895. FCD. 1 hit.
SM00345. HTH_GNTR. 1 hit.
[Graphical view]
SUPFAMSSF48008. SSF48008. 1 hit.
PROSITEPS50949. HTH_GNTR. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

PROP76114.

Entry information

Entry nameMCBR_ECOLI
AccessionPrimary (citable) accession number: P76114
Secondary accession number(s): P77347
Entry history
Integrated into UniProtKB/Swiss-Prot: July 15, 1999
Last sequence update: July 15, 1999
Last modified: May 14, 2014
This is version 112 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Escherichia coli

Escherichia coli (strain K12): entries and cross-references to EcoGene