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P76114

- MCBR_ECOLI

UniProt

P76114 - MCBR_ECOLI

Protein

HTH-type transcriptional regulator McbR

Gene

mcbR

Organism
Escherichia coli (strain K12)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 113 (01 Oct 2014)
      Sequence version 2 (15 Jul 1999)
      Previous versions | rss
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    Functioni

    Important for biofilm formation. Represses expression of McbA by binding to its promoter region, which prevents colanic acid overproduction and mucoidy.1 Publication

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    DNA bindingi37 – 5620H-T-H motifPROSITE-ProRule annotationAdd
    BLAST

    GO - Molecular functioni

    1. DNA binding Source: UniProtKB-KW
    2. sequence-specific DNA binding transcription factor activity Source: EcoCyc

    GO - Biological processi

    1. negative regulation of transcription, DNA-templated Source: EcoCyc
    2. transcription, DNA-templated Source: UniProtKB-KW

    Keywords - Molecular functioni

    Repressor

    Keywords - Biological processi

    Transcription, Transcription regulation

    Keywords - Ligandi

    DNA-binding

    Enzyme and pathway databases

    BioCyciEcoCyc:G6761-MONOMER.
    ECOL316407:JW1445-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    HTH-type transcriptional regulator McbR
    Gene namesi
    Name:mcbR
    Synonyms:yncC
    Ordered Locus Names:b1450, JW1445
    OrganismiEscherichia coli (strain K12)
    Taxonomic identifieri83333 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
    ProteomesiUP000000318: Chromosome, UP000000625: Chromosome

    Organism-specific databases

    EcoGeneiEG13773. mcbR.

    Pathology & Biotechi

    Disruption phenotypei

    Disruption of this gene induces 30 genes and represses 10 genes more than fourfold. It decreases biofilm formation, increases extracellular indole, leads to colanic acid overproduction and elicits mucoidy.1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 221221HTH-type transcriptional regulator McbRPRO_0000050686Add
    BLAST

    Proteomic databases

    PaxDbiP76114.
    PRIDEiP76114.

    Expressioni

    Inductioni

    Induced by LsrR, LsrK, MqsR and TqsA. Repressed by LuxS and YgiV.1 Publication

    Gene expression databases

    GenevestigatoriP76114.

    Interactioni

    Protein-protein interaction databases

    DIPiDIP-12749N.
    IntActiP76114. 11 interactions.
    MINTiMINT-1303162.
    STRINGi511145.b1450.

    Structurei

    3D structure databases

    ProteinModelPortaliP76114.
    SMRiP76114. Positions 4-219.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini10 – 7768HTH gntR-typePROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Contains 1 HTH gntR-type DNA-binding domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiCOG1802.
    HOGENOMiHOG000273993.
    KOiK13654.
    OMAiERIENYH.
    OrthoDBiEOG6JX7PH.
    PhylomeDBiP76114.

    Family and domain databases

    Gene3Di1.10.10.10. 1 hit.
    1.20.120.530. 1 hit.
    InterProiIPR011711. GntR_C.
    IPR008920. TF_FadR/GntR_C.
    IPR000524. Tscrpt_reg_HTH_GntR.
    IPR011991. WHTH_DNA-bd_dom.
    [Graphical view]
    PfamiPF07729. FCD. 1 hit.
    PF00392. GntR. 1 hit.
    [Graphical view]
    SMARTiSM00895. FCD. 1 hit.
    SM00345. HTH_GNTR. 1 hit.
    [Graphical view]
    SUPFAMiSSF48008. SSF48008. 1 hit.
    PROSITEiPS50949. HTH_GNTR. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P76114-1 [UniParc]FASTAAdd to Basket

    « Hide

    MPGTGKMKHV SLTLQVENDL KHQLSIGALK PGARLITKNL AEQLGMSITP    50
    VREALLRLVS VNALSVAPAQ AFTVPEVGKR QLDEINRIRY ELELMAVALA 100
    VENLTPQDLA ELQELLEKLQ QAQEKGDMEQ IINVNRLFRL AIYHRSNMPI 150
    LCEMIEQLWV RMGPGLHYLY EAINPAELRE HIENYHLLLA ALKAKDKEGC 200
    RHCLAEIMQQ NIAILYQQYN R 221
    Length:221
    Mass (Da):25,151
    Last modified:July 15, 1999 - v2
    Checksum:i7A589BCFAE20D75C
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U00096 Genomic DNA. Translation: AAC74532.2.
    AP009048 Genomic DNA. Translation: BAA15082.1.
    PIRiE64897.
    RefSeqiNP_415967.2. NC_000913.3.
    YP_489715.1. NC_007779.1.

    Genome annotation databases

    EnsemblBacteriaiAAC74532; AAC74532; b1450.
    BAA15082; BAA15082; BAA15082.
    GeneIDi12931202.
    946014.
    KEGGiecj:Y75_p1426.
    eco:b1450.
    PATRICi32118192. VBIEscCol129921_1516.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U00096 Genomic DNA. Translation: AAC74532.2 .
    AP009048 Genomic DNA. Translation: BAA15082.1 .
    PIRi E64897.
    RefSeqi NP_415967.2. NC_000913.3.
    YP_489715.1. NC_007779.1.

    3D structure databases

    ProteinModelPortali P76114.
    SMRi P76114. Positions 4-219.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    DIPi DIP-12749N.
    IntActi P76114. 11 interactions.
    MINTi MINT-1303162.
    STRINGi 511145.b1450.

    Proteomic databases

    PaxDbi P76114.
    PRIDEi P76114.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAC74532 ; AAC74532 ; b1450 .
    BAA15082 ; BAA15082 ; BAA15082 .
    GeneIDi 12931202.
    946014.
    KEGGi ecj:Y75_p1426.
    eco:b1450.
    PATRICi 32118192. VBIEscCol129921_1516.

    Organism-specific databases

    EchoBASEi EB3535.
    EcoGenei EG13773. mcbR.

    Phylogenomic databases

    eggNOGi COG1802.
    HOGENOMi HOG000273993.
    KOi K13654.
    OMAi ERIENYH.
    OrthoDBi EOG6JX7PH.
    PhylomeDBi P76114.

    Enzyme and pathway databases

    BioCyci EcoCyc:G6761-MONOMER.
    ECOL316407:JW1445-MONOMER.

    Miscellaneous databases

    PROi P76114.

    Gene expression databases

    Genevestigatori P76114.

    Family and domain databases

    Gene3Di 1.10.10.10. 1 hit.
    1.20.120.530. 1 hit.
    InterProi IPR011711. GntR_C.
    IPR008920. TF_FadR/GntR_C.
    IPR000524. Tscrpt_reg_HTH_GntR.
    IPR011991. WHTH_DNA-bd_dom.
    [Graphical view ]
    Pfami PF07729. FCD. 1 hit.
    PF00392. GntR. 1 hit.
    [Graphical view ]
    SMARTi SM00895. FCD. 1 hit.
    SM00345. HTH_GNTR. 1 hit.
    [Graphical view ]
    SUPFAMi SSF48008. SSF48008. 1 hit.
    PROSITEi PS50949. HTH_GNTR. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / MG1655 / ATCC 47076.
    3. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
      Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
      Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
    4. "Escherichia coli transcription factor YncC (McbR) regulates colanic acid and biofilm formation by repressing expression of periplasmic protein YbiM (McbA)."
      Zhang X.-S., Garcia-Contreras R., Wood T.K.
      ISME J. 2:615-631(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION IN BIOFILM FORMATION, INDUCTION, DISRUPTION PHENOTYPE.
      Strain: K12 / BW25113.

    Entry informationi

    Entry nameiMCBR_ECOLI
    AccessioniPrimary (citable) accession number: P76114
    Secondary accession number(s): P77347
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 15, 1999
    Last sequence update: July 15, 1999
    Last modified: October 1, 2014
    This is version 113 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Escherichia coli
      Escherichia coli (strain K12): entries and cross-references to EcoGene
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3