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Protein

NADPH-dependent curcumin reductase

Gene

curA

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the metal-independent reduction of curcumin to dihydrocurcumin (DHC) as an intermediate product, followed by further reduction to tetrahydrocurcumin (THC) as an end product. It also acts on 3-octene-2-one, 3-hepten-2-one, resveratrol, and trans-2-octenal.1 Publication

Catalytic activityi

Tetrahydrocurcumin + 2 NADP+ = curcumin + 2 NADPH.1 Publication

Enzyme regulationi

Inhibited by thiol-specific reagents (p-chloromercuribenzoate and 5,5'-dithio-bis-2-nitrobenzoate).1 Publication

Kineticsi

  1. KM=29 µM for curcumin1 Publication
  1. Vmax=9.35 µmol/min/mg enzyme1 Publication

pH dependencei

Optimum pH is 5.9. The enzyme is most stable in the pH range of 4.5 to 12.0, with 90% of the initial activity being retained even at pH 12.0.1 Publication

Temperature dependencei

Optimum temperature is 35 degrees Celsius. The enzyme is most stable from 10 to 50 degrees Celsius.1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei51 – 511NADPBy similarity
Binding sitei186 – 1861NADPBy similarity
Binding sitei225 – 2251NADPBy similarity
Binding sitei333 – 3331NADPBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi160 – 1634NADPBy similarity
Nucleotide bindingi182 – 1865NADPBy similarity
Nucleotide bindingi247 – 2537NADPBy similarity
Nucleotide bindingi282 – 2843NADPBy similarity

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Ligandi

NADP

Enzyme and pathway databases

BioCyciEcoCyc:G6760-MONOMER.
ECOL316407:JW5907-MONOMER.
MetaCyc:G6760-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
NADPH-dependent curcumin reductase (EC:1.3.1.n3)
Alternative name(s):
NADPH-dependent curcumin/dihydrocurcumin reductase
Gene namesi
Name:curA
Synonyms:yncB
Ordered Locus Names:b1449, JW5907
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG13772. curA.

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi247 – 2471C → A: Exhibits a reduction of about 50% in activity, but is not inhibited by 5,5'-dithio-bis-2-nitrobenzoate. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 345345NADPH-dependent curcumin reductasePRO_0000218075Add
BLAST

Proteomic databases

EPDiP76113.
PaxDbiP76113.

Interactioni

Subunit structurei

Homodimer.1 Publication

Protein-protein interaction databases

BioGridi4260195. 6 interactions.
DIPiDIP-12748N.
STRINGi511145.b1449.

Structurei

3D structure databases

ProteinModelPortaliP76113.
SMRiP76113. Positions 6-341.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiENOG4105CJH. Bacteria.
COG2130. LUCA.
HOGENOMiHOG000294663.
InParanoidiP76113.
KOiK07119.
OrthoDBiEOG6MSS2K.
PhylomeDBiP76113.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
3.90.180.10. 1 hit.
InterProiIPR013149. ADH_C.
IPR002085. ADH_SF_Zn-type.
IPR011032. GroES-like.
IPR016040. NAD(P)-bd_dom.
IPR020843. PKS_ER.
[Graphical view]
PANTHERiPTHR11695. PTHR11695. 1 hit.
PfamiPF00107. ADH_zinc_N. 1 hit.
[Graphical view]
SMARTiSM00829. PKS_ER. 1 hit.
[Graphical view]
SUPFAMiSSF50129. SSF50129. 2 hits.
SSF51735. SSF51735. 1 hit.

Sequencei

Sequence statusi: Complete.

P76113-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGQQKQRNRR WVLASRPHGA PVPENFRLEE DDVATPGEGQ VLLRTVYLSL
60 70 80 90 100
DPYMRGRMSD EPSYSPPVDI GGVMVGGTVS RVVESNHPDY QSGDWVLGYS
110 120 130 140 150
GWQDYDISSG DDLVKLGDHP QNPSWSLGVL GMPGFTAYMG LLDIGQPKEG
160 170 180 190 200
ETLVVAAATG PVGATVGQIG KLKGCRVVGV AGGAEKCRHA TEVLGFDVCL
210 220 230 240 250
DHHADDFAEQ LAKACPKGID IYYENVGGKV FDAVLPLLNT SARIPVCGLV
260 270 280 290 300
SSYNATELPP GPDRLPLLMA TVLKKRIRLQ GFIIAQDYGH RIHEFQREMG
310 320 330 340
QWVKEDKIHY REEITDGLEN APQTFIGLLK GKNFGKVVIR VAGDD
Length:345
Mass (Da):37,610
Last modified:November 24, 2009 - v3
Checksum:iCED3CF29B60E246F
GO

Mass spectrometryi

Molecular mass is 37513 Da from positions 1 - 345. Determined by MALDI. 1 Publication

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00096 Genomic DNA. Translation: AAC74531.4.
AP009048 Genomic DNA. Translation: BAA15081.1.
PIRiD64897.
RefSeqiNP_415966.6. NC_000913.3.
WP_000531462.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC74531; AAC74531; b1449.
BAA15081; BAA15081; BAA15081.
GeneIDi946012.
KEGGiecj:JW5907.
eco:b1449.
PATRICi32118190. VBIEscCol129921_1515.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00096 Genomic DNA. Translation: AAC74531.4.
AP009048 Genomic DNA. Translation: BAA15081.1.
PIRiD64897.
RefSeqiNP_415966.6. NC_000913.3.
WP_000531462.1. NZ_LN832404.1.

3D structure databases

ProteinModelPortaliP76113.
SMRiP76113. Positions 6-341.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4260195. 6 interactions.
DIPiDIP-12748N.
STRINGi511145.b1449.

Proteomic databases

EPDiP76113.
PaxDbiP76113.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC74531; AAC74531; b1449.
BAA15081; BAA15081; BAA15081.
GeneIDi946012.
KEGGiecj:JW5907.
eco:b1449.
PATRICi32118190. VBIEscCol129921_1515.

Organism-specific databases

EchoBASEiEB3534.
EcoGeneiEG13772. curA.

Phylogenomic databases

eggNOGiENOG4105CJH. Bacteria.
COG2130. LUCA.
HOGENOMiHOG000294663.
InParanoidiP76113.
KOiK07119.
OrthoDBiEOG6MSS2K.
PhylomeDBiP76113.

Enzyme and pathway databases

BioCyciEcoCyc:G6760-MONOMER.
ECOL316407:JW5907-MONOMER.
MetaCyc:G6760-MONOMER.

Miscellaneous databases

PROiP76113.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
3.90.180.10. 1 hit.
InterProiIPR013149. ADH_C.
IPR002085. ADH_SF_Zn-type.
IPR011032. GroES-like.
IPR016040. NAD(P)-bd_dom.
IPR020843. PKS_ER.
[Graphical view]
PANTHERiPTHR11695. PTHR11695. 1 hit.
PfamiPF00107. ADH_zinc_N. 1 hit.
[Graphical view]
SMARTiSM00829. PKS_ER. 1 hit.
[Graphical view]
SUPFAMiSSF50129. SSF50129. 2 hits.
SSF51735. SSF51735. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  3. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
    Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
    Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  4. "Discovery of the curcumin metabolic pathway involving a unique enzyme in an intestinal microorganism."
    Hassaninasab A., Hashimoto Y., Tomita-Yokotani K., Kobayashi M.
    Proc. Natl. Acad. Sci. U.S.A. 108:6615-6620(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION AS A CURCUMIN REDUCTASE, CATALYTIC ACTIVITY, MASS SPECTROMETRY, MUTAGENESIS OF CYS-247, SUBSTRATE SPECIFICITY, BIOPHYSICOCHEMICAL PROPERTIES, ENZYME REGULATION, SUBUNIT, NOMENCLATURE.

Entry informationi

Entry nameiCURA_ECOLI
AccessioniPrimary (citable) accession number: P76113
Secondary accession number(s): P78255
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 24, 2009
Last modified: May 11, 2016
This is version 109 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.