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Protein

L-amino acid N-acyltransferase MnaT

Gene

mnaT

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

N-acylates some free L-amino acids and their derivatives using a CoA thioester as cofactor. Using acetyl-CoA as a cofactor, substrate specificity is methionine sulfone > methionine sulfoximine > methionine sulfoxide > methionine. Asparagine, lysine, glutamine, aspartate and glutamate are very poor substrates. Using methionine as a substrate, cofactor preference is propionyl-CoA > acetyl-CoA >> butyryl-CoA.1 Publication

Catalytic activityi

Acetyl-CoA + L-methionine = CoA + N-acetyl-L-methionine.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Acyltransferase, Transferase

Enzyme and pathway databases

BioCyciEcoCyc:G6759-MONOMER.
ECOL316407:JW5233-MONOMER.
MetaCyc:G6759-MONOMER.
RETL1328306-WGS:GSTH-5732-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
L-amino acid N-acyltransferase MnaT (EC:2.3.1.-)
Gene namesi
Name:mnaT
Synonyms:yncA
Ordered Locus Names:b1448, JW5233
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG13770. mnaT.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Pathology & Biotechi

Biotechnological usei

Expression leads to increased levels of N-acylated L-amino acids which could be used in a number of applications.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 172172L-amino acid N-acyltransferase MnaTPRO_0000074577Add
BLAST

Proteomic databases

PaxDbiP76112.

Interactioni

Protein-protein interaction databases

BioGridi4260194. 12 interactions.
STRINGi511145.b1448.

Structurei

3D structure databases

ProteinModelPortaliP76112.
SMRiP76112. Positions 1-170.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini1 – 163163N-acetyltransferasePROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 N-acetyltransferase domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiENOG4108Z7F. Bacteria.
COG1247. LUCA.
HOGENOMiHOG000078516.
InParanoidiP76112.
KOiK03823.
OMAiVTGQMPQ.
PhylomeDBiP76112.

Family and domain databases

Gene3Di3.40.630.30. 1 hit.
InterProiIPR016181. Acyl_CoA_acyltransferase.
IPR000182. GNAT_dom.
[Graphical view]
SUPFAMiSSF55729. SSF55729. 1 hit.
PROSITEiPS51186. GNAT. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P76112-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSIRFARKAD CAAIAEIYNH AVLYTAAIWN DQTVDADNRI AWFEARTLAG
60 70 80 90 100
YPVLVSEENG VVTGYASFGD WRSFDGFRHT VEHSVYVHPD HQGKGLGRKL
110 120 130 140 150
LSRLIDEARD CGKHVMVAGI ESQNQASLHL HQSLGFVVTA QMPQVGTKFG
160 170
RWLDLTFMQL QLDERTEPDA IG
Length:172
Mass (Da):19,248
Last modified:February 1, 1997 - v1
Checksum:i4DAA0DC5198CDBD1
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00096 Genomic DNA. Translation: AAC74530.1.
AP009048 Genomic DNA. Translation: BAA15080.2.
PIRiC64897.
RefSeqiNP_415965.1. NC_000913.3.
WP_001310799.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC74530; AAC74530; b1448.
BAA15080; BAA15080; BAA15080.
GeneIDi946010.
KEGGiecj:JW5233.
eco:b1448.
PATRICi32118188. VBIEscCol129921_1514.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00096 Genomic DNA. Translation: AAC74530.1.
AP009048 Genomic DNA. Translation: BAA15080.2.
PIRiC64897.
RefSeqiNP_415965.1. NC_000913.3.
WP_001310799.1. NZ_LN832404.1.

3D structure databases

ProteinModelPortaliP76112.
SMRiP76112. Positions 1-170.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4260194. 12 interactions.
STRINGi511145.b1448.

Proteomic databases

PaxDbiP76112.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC74530; AAC74530; b1448.
BAA15080; BAA15080; BAA15080.
GeneIDi946010.
KEGGiecj:JW5233.
eco:b1448.
PATRICi32118188. VBIEscCol129921_1514.

Organism-specific databases

EchoBASEiEB3533.
EcoGeneiEG13770. mnaT.

Phylogenomic databases

eggNOGiENOG4108Z7F. Bacteria.
COG1247. LUCA.
HOGENOMiHOG000078516.
InParanoidiP76112.
KOiK03823.
OMAiVTGQMPQ.
PhylomeDBiP76112.

Enzyme and pathway databases

BioCyciEcoCyc:G6759-MONOMER.
ECOL316407:JW5233-MONOMER.
MetaCyc:G6759-MONOMER.
RETL1328306-WGS:GSTH-5732-MONOMER.

Miscellaneous databases

PROiP76112.

Family and domain databases

Gene3Di3.40.630.30. 1 hit.
InterProiIPR016181. Acyl_CoA_acyltransferase.
IPR000182. GNAT_dom.
[Graphical view]
SUPFAMiSSF55729. SSF55729. 1 hit.
PROSITEiPS51186. GNAT. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiMNAT_ECOLI
AccessioniPrimary (citable) accession number: P76112
Secondary accession number(s): P77401
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: February 1, 1997
Last modified: September 7, 2016
This is version 110 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.