P76112 (YNCA_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
N-acyltransferase YncA
EC=2.3.1.-

Gene names

Name:	yncA
Ordered Locus Names:	b1448, JW5233

Organism

Escherichia coli (strain K12) [Reference proteome] [HAMAP]

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia › Escherichia coli

Protein attributes

Sequence length	172 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	N-acylates some free L-amino acids and their derivatives using a CoA thioester as cofactor. Using acetyl-CoA as a cofactor, substrate specificity is methionine sulfone > methionine sulfoximine > methionine sulfoxide > methionine. Asparagine, lysine, glutamine, aspartate and glutamate are very poor substrates. Using methionine as a substrate, cofactor preference is propionyl-CoA > acetyl-CoA >> butyryl-CoA. Ref.4
Catalytic activity	Acetyl-CoA + L-methionine = CoA + N-acetyl-L-methionine.
Biotechnological use	Expression leads to increased levels of N-acylated L-amino acids which could be used in a number of applications. Ref.4
Sequence similarities	Belongs to the acetyltransferase family. PAT/BAR subfamily. Contains 1 N-acetyltransferase domain.

Ontologies

Keywords
Molecular function	Acyltransferase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Molecular_function	N-acetyltransferase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 172	172	N-acyltransferase YncA		PRO_0000074577
Regions
	Domain	1 – 163	163	N-acetyltransferase

Sequences

	Sequence		Length	Mass (Da)
	P76112 [UniParc]. Last modified February 1, 1997. Version 1. Checksum: 4DAA0DC5198CDBD1	FASTA	172	19,248
	10 20 30 40 50 60 MSIRFARKAD CAAIAEIYNH AVLYTAAIWN DQTVDADNRI AWFEARTLAG YPVLVSEENG 70 80 90 100 110 120 VVTGYASFGD WRSFDGFRHT VEHSVYVHPD HQGKGLGRKL LSRLIDEARD CGKHVMVAGI 130 140 150 160 170 ESQNQASLHL HQSLGFVVTA QMPQVGTKFG RWLDLTFMQL QLDERTEPDA IG

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"A 570-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 28.0-40.1 min region on the linkage map." Aiba H., Baba T., Fujita K., Hayashi K., Inada T., Isono K., Itoh T., Kasai H., Kashimoto K., Kimura S., Kitakawa M., Kitagawa M., Makino K., Miki T., Mizobuchi K., Mori H., Mori T., Motomura K. , Nakade S., Nakamura Y., Nashimoto H., Nishio Y., Oshima T., Saito N., Sampei G., Seki Y., Sivasundaram S., Tagami H., Takeda J., Takemoto K., Takeuchi Y., Wada C., Yamamoto Y., Horiuchi T. DNA Res. 3:363-377(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[2]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[3]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[4]	"Production of N-acylated sulphur-containing amino acids with microorganisms having enhanced N-acyltransferase enzymatic activity." Figge R., Barbier G., Bestel-Corre G. Patent number US0047880, 22-AUG-2008 Cited for: FUNCTION AS AN ACYLTRANSFERASE, BIOTECHNOLOGY. Strain: K12 / MG1655 / ATCC 47076.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U00096 Genomic DNA. Translation: AAC74530.1. AP009048 Genomic DNA. Translation: BAA15080.2.
PIR	C64897.
RefSeq	NP_415965.1. NC_000913.2. YP_489713.1. NC_007779.1.
3D structure databases
ProteinModelPortal	P76112.
SMR	P76112. Positions 1-170.
ModBase	Search...
Protein-protein interaction databases
STRING	511145.b1448.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAC74530; AAC74530; b1448. BAA15080; BAA15080; BAA15080.
GeneID	12931200. 946010.
KEGG	ecj:Y75_p1424. eco:b1448.
PATRIC	32118188. VBIEscCol129921_1514.
Organism-specific databases
EchoBASE	EB3533.
EcoGene	EG13770. yncA.
Phylogenomic databases
eggNOG	COG1247.
HOGENOM	HOG000078516.
KO	K03823.
OMA	HAVEHTT.
ProtClustDB	CLSK880062.
Enzyme and pathway databases
BioCyc	EcoCyc:G6759-MONOMER. ECOL316407:JW5233-MONOMER. MetaCyc:G6759-MONOMER.
Gene expression databases
Genevestigator	P76112.
Family and domain databases
Gene3D	3.40.630.30. 1 hit.
InterPro	IPR016181. Acyl_CoA_acyltransferase. IPR000182. GNAT_dom. [Graphical view]
Pfam	PF00583. Acetyltransf_1. 1 hit. [Graphical view]
SUPFAM	SSF55729. Acyl_CoA_acyltransferase. 1 hit.
PROSITE	PS51186. GNAT. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

YNCA_ECOLI

Accession

Primary (citable) accession number: P76112
Secondary accession number(s): P77401

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	February 1, 1997
Last modified:	May 1, 2013
This is version 89 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program