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Protein

Bifunctional polyhydroxybutyrate synthase / ABC transporter periplasmic binding protein

Gene

ydcS

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes the formation of short polymers of R-3-hydroxybutyrate (cPHB) (PubMed:18640095). Involved in natural transformation (PubMed:26826386). Probably part of the ABC transporter complex YdcSTUV. During natural transformation, may bind dsDNA and convey it to the inner membrane channel formed by YdcV (Probable).1 Publication2 Publications

Catalytic activityi

Converts D--3-hydroxybutyryl-CoA to polyhydroxybutyrate + CoA.1 Publication

Kineticsi

  1. KM=0.14 mM for 3-hydroxybutyryl-CoA1 Publication
  1. Vmax=18.7 nmol/min/mg enzyme1 Publication

pH dependencei

Optimum pH is 6.8-7.8.1 Publication

Temperature dependencei

Optimum temperature is 37 degrees Celsius.1 Publication

GO - Molecular functioni

GO - Biological processi

  • DNA import into cell involved in transformation Source: EcoCyc
  • polyamine transport Source: InterPro
  • poly-hydroxybutyrate biosynthetic process Source: EcoCyc

Keywordsi

Molecular functionAcyltransferase, Transferase
Biological processPHB biosynthesis, Transport

Enzyme and pathway databases

BioCyciEcoCyc:YDCS-MONOMER
MetaCyc:YDCS-MONOMER

Protein family/group databases

TCDBi3.A.1.11.9 the atp-binding cassette (abc) superfamily

Names & Taxonomyi

Protein namesi
Recommended name:
Bifunctional polyhydroxybutyrate synthase / ABC transporter periplasmic binding proteinCurated
Alternative name(s):
Poly-3-hydroxybutyrate synthase1 Publication (EC:2.3.1.-1 Publication)
Short name:
PHB synthase1 Publication
cPHB synthase1 Publication
Gene namesi
Name:ydcS
Ordered Locus Names:b1440, JW1435
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG13762 ydcS

Subcellular locationi

  • Periplasm 1 Publication

GO - Cellular componenti

  • outer membrane-bounded periplasmic space Source: EcoCyc

Keywords - Cellular componenti

Periplasm

Pathology & Biotechi

Disruption phenotypei

The deletion mutant contains 30% less cPHB in the outer membrane than the wild-type strain (PubMed:18640095). Natural transformation rate of the mutant is reduced by 2.7-6.7 folds. Mutant is also defective in chemical transformation (PubMed:26826386).2 Publications

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 22Sequence analysisAdd BLAST22
ChainiPRO_000003184323 – 381Bifunctional polyhydroxybutyrate synthase / ABC transporter periplasmic binding proteinAdd BLAST359

Proteomic databases

EPDiP76108
PaxDbiP76108
PRIDEiP76108

Expressioni

Inductioni

Induced under nitrogen-limited growth.1 Publication

Interactioni

Protein-protein interaction databases

BioGridi4262896, 55 interactors
IntActiP76108, 19 interactors
STRINGi316385.ECDH10B_1570

Structurei

3D structure databases

ProteinModelPortaliP76108
SMRiP76108
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiENOG4107TYH Bacteria
ENOG410XUAZ LUCA
HOGENOMiHOG000283673
KOiK02055
OMAiDWVTGFE
PhylomeDBiP76108

Family and domain databases

InterProiView protein in InterPro
IPR006059 SBP
IPR001188 Sperm_putr-bd
PfamiView protein in Pfam
PF13416 SBP_bac_8, 1 hit
PRINTSiPR00909 SPERMDNBNDNG

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P76108-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSKTFARSSL CALSMTIMTA HAAEPPTNLD KPEGRLDIIA WPGYIERGQT
60 70 80 90 100
DKQYDWVTQF EKETGCAVNV KTAATSDEMV SLMTKGGYDL VTASGDASLR
110 120 130 140 150
LIMGKRVQPI NTALIPNWKT LDPRVVKGDW FNVGGKVYGT PYQWGPNLLM
160 170 180 190 200
YNTKTFPTPP DSWQVVFVEQ NLPDGKSNKG RVQAYDGPIY IADAALFVKA
210 220 230 240 250
TQPQLGISDP YQLTEEQYQA VLKVLRAQHS LIHRYWHDTT VQMSDFKNEG
260 270 280 290 300
VVASSAWPYQ ANALKAEGQP VATVFPKEGV TGWADTTMLH SEAKHPVCAY
310 320 330 340 350
KWMNWSLTPK VQGDVAAWFG SLPVVPEGCK ASPLLGEKGC ETNGFNYFDK
360 370 380
IAFWKTPIAE GGKFVPYSRW TQDYIAIMGG R
Length:381
Mass (Da):42,295
Last modified:February 1, 1997 - v1
Checksum:iFC5072B0B52CFB05
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00096 Genomic DNA Translation: AAC74522.1
AP009048 Genomic DNA Translation: BAE76440.1
PIRiC64896
RefSeqiNP_415957.1, NC_000913.3
WP_000047424.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaiAAC74522; AAC74522; b1440
BAE76440; BAE76440; BAE76440
GeneIDi946005
KEGGiecj:JW1435
eco:b1440
PATRICifig|1411691.4.peg.828

Similar proteinsi

Entry informationi

Entry nameiYDCS_ECOLI
AccessioniPrimary (citable) accession number: P76108
Secondary accession number(s): Q2MBB6
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: February 1, 1997
Last modified: April 25, 2018
This is version 126 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families

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