ID   PAAX_ECOLI              Reviewed;         316 AA.
AC   P76086; O53019; Q2MBC7;
DT   15-JUL-1998, integrated into UniProtKB/Swiss-Prot.
DT   01-FEB-1997, sequence version 1.
DT   24-JAN-2024, entry version 146.
DE   RecName: Full=Transcriptional repressor PaaX;
GN   Name=paaX; Synonyms=ydbY; OrderedLocusNames=b1399, JW1394;
OS   Escherichia coli (strain K12).
OC   Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=83333;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND FUNCTION IN PHENYLACETATE
RP   CATABOLISM.
RC   STRAIN=W / ATCC 11105 / DSM 1900;
RX   PubMed=9748275; DOI=10.1074/jbc.273.40.25974;
RA   Ferrandez A., Minambres B., Garcia B., Olivera E.R., Luengo J.M.,
RA   Garcia J.L., Diaz E.;
RT   "Catabolism of phenylacetic acid in Escherichia coli. Characterization of a
RT   new aerobic hybrid pathway.";
RL   J. Biol. Chem. 273:25974-25986(1998).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=K12 / MG1655 / ATCC 47076;
RX   PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA   Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA   Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA   Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B.,
RA   Shao Y.;
RT   "The complete genome sequence of Escherichia coli K-12.";
RL   Science 277:1453-1462(1997).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX   PubMed=16738553; DOI=10.1038/msb4100049;
RA   Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S.,
RA   Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
RT   "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655
RT   and W3110.";
RL   Mol. Syst. Biol. 2:E1-E5(2006).
RN   [4]
RP   FUNCTION AS A TRANSCRIPTIONAL REPRESSOR, REGULATION BY PA-COA, AND
RP   CONSENSUS MOTIF.
RX   PubMed=10766858; DOI=10.1074/jbc.275.16.12214;
RA   Ferrandez A., Garcia J.L., Diaz E.;
RT   "Transcriptional regulation of the divergent paa catabolic operons for
RT   phenylacetic acid degradation in Escherichia coli.";
RL   J. Biol. Chem. 275:12214-12222(2000).
CC   -!- FUNCTION: Negative regulator of the paaZ and paaABCDEFGHIJK catabolic
CC       operons. Binds the consensus sequence 5'-WWTRTGATTCGYGWT-3'. Binding of
CC       PaaX is specifically inhibited by phenylacetyl-coenzyme A (PA-CoA).
CC       {ECO:0000269|PubMed:10766858, ECO:0000269|PubMed:9748275}.
CC   -!- PATHWAY: Aromatic compound metabolism; phenylacetate degradation.
CC   -!- INTERACTION:
CC       P76086; P0AE82: cpxA; NbExp=3; IntAct=EBI-544692, EBI-9141330;
CC       P76086; P41443: gspH; NbExp=2; IntAct=EBI-544692, EBI-1129978;
CC   -!- SIMILARITY: Belongs to the PaaX family. {ECO:0000305}.
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DR   EMBL; X97452; CAA66101.1; -; Genomic_DNA.
DR   EMBL; U00096; AAC74481.1; -; Genomic_DNA.
DR   EMBL; AP009048; BAE76429.1; -; Genomic_DNA.
DR   PIR; B64891; B64891.
DR   RefSeq; NP_415917.1; NC_000913.3.
DR   RefSeq; WP_000039887.1; NZ_SSZK01000012.1.
DR   PDB; 8A39; X-ray; 2.30 A; AP1/BP1/CP1=1-316.
DR   PDBsum; 8A39; -.
DR   AlphaFoldDB; P76086; -.
DR   SMR; P76086; -.
DR   BioGRID; 4263014; 111.
DR   BioGRID; 850329; 6.
DR   DIP; DIP-10431N; -.
DR   IntAct; P76086; 21.
DR   STRING; 511145.b1399; -.
DR   jPOST; P76086; -.
DR   PaxDb; 511145-b1399; -.
DR   EnsemblBacteria; AAC74481; AAC74481; b1399.
DR   GeneID; 945966; -.
DR   KEGG; ecj:JW1394; -.
DR   KEGG; eco:b1399; -.
DR   PATRIC; fig|1411691.4.peg.872; -.
DR   EchoBASE; EB3509; -.
DR   eggNOG; COG3327; Bacteria.
DR   HOGENOM; CLU_067515_0_0_6; -.
DR   InParanoid; P76086; -.
DR   OMA; LLIHEYR; -.
DR   OrthoDB; 2270427at2; -.
DR   PhylomeDB; P76086; -.
DR   BioCyc; EcoCyc:G6720-MONOMER; -.
DR   UniPathway; UPA00930; -.
DR   PRO; PR:P76086; -.
DR   Proteomes; UP000000318; Chromosome.
DR   Proteomes; UP000000625; Chromosome.
DR   GO; GO:0000976; F:transcription cis-regulatory region binding; IDA:EcoCyc.
DR   GO; GO:0006351; P:DNA-templated transcription; IDA:EcoCyc.
DR   GO; GO:2000143; P:negative regulation of DNA-templated transcription initiation; IMP:EcoCyc.
DR   GO; GO:0010124; P:phenylacetate catabolic process; IMP:UniProtKB.
DR   Gene3D; 1.20.58.1460; -; 1.
DR   Gene3D; 1.10.10.10; Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain; 1.
DR   InterPro; IPR048846; PaaX-like_central.
DR   InterPro; IPR012906; PaaX-like_N.
DR   InterPro; IPR013225; PaaX_C.
DR   InterPro; IPR011965; PaaX_trns_reg.
DR   InterPro; IPR036388; WH-like_DNA-bd_sf.
DR   NCBIfam; TIGR02277; PaaX_trns_reg; 1.
DR   PANTHER; PTHR30319; PHENYLACETIC ACID REGULATOR-RELATED TRANSCRIPTIONAL REPRESSOR; 1.
DR   PANTHER; PTHR30319:SF1; TRANSCRIPTIONAL REPRESSOR PAAX; 1.
DR   Pfam; PF07848; PaaX; 1.
DR   Pfam; PF08223; PaaX_C; 1.
DR   Pfam; PF20803; PaaX_M; 1.
DR   PIRSF; PIRSF020623; PaaX; 1.
PE   1: Evidence at protein level;
KW   3D-structure; DNA-binding; Reference proteome; Repressor; Transcription;
KW   Transcription regulation.
FT   CHAIN           1..316
FT                   /note="Transcriptional repressor PaaX"
FT                   /id="PRO_0000058165"
FT   VARIANT         5
FT                   /note="D -> V (in strain: W)"
FT   VARIANT         311
FT                   /note="L -> I (in strain: W)"
FT   VARIANT         315
FT                   /note="I -> T (in strain: W)"
SQ   SEQUENCE   316 AA;  35295 MW;  286C97DBC2564030 CRC64;
     MSKLDTFIQH AVNAVPVSGT SLISSLYGDS LSHRGGEIWL GSLAALLEGL GFGERFVRTA
     LFRLNKEGWL DVSRIGRRSF YSLSDKGLRL TRRAESKIYR AEQPAWDGKW LLLLSEGLDK
     STLADVKKQL IWQGFGALAP SLMASPSQKL ADVQTLLHEA GVADNVICFE AQIPLALSRA
     ALRARVEECW HLTEQNAMYE TFIQSFRPLV PLLKEAADEL TPERAFHIQL LLIHFYRRVV
     LKDPLLPEEL LPAHWAGHTA RQLCINIYQR VAPAALAFVS EKGETSVGEL PAPGSLYFQR
     FGGLNIEQEA LCQFIR
//