P76086 (PAAX_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Transcriptional repressor PaaX

Gene names

Name:	paaX
Synonyms:	ydbY
Ordered Locus Names:	b1399, JW1394

Organism

Escherichia coli (strain K12) [Reference proteome] [HAMAP]

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia › Escherichia coli

Protein attributes

Sequence length	316 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Negative regulator of the paaZ and paaABCDEFGHIJK catabolic operons. Binds the consensus sequence 5'-WWTRTGATTCGYGWT-3'. Binding of PaaX is specifically inhibited by phenylacetyl-coenzyme A (PA-CoA). Ref.1 Ref.4
Pathway	Aromatic compound metabolism; phenylacetate degradation.
Sequence similarities	Belongs to the PaaX family.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Ligand	DNA-binding
Molecular function	Repressor
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	phenylacetate catabolic process Inferred from mutant phenotype Ref.1. Source: UniProtKB regulation of transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW transcription, DNA-dependent Inferred from genetic interaction Ref.4. Source: EcoCyc
Molecular_function	DNA binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 316	316	Transcriptional repressor PaaX		PRO_0000058165
Natural variations
	Natural variant	5	1	D → V in strain: W.
	Natural variant	311	1	L → I in strain: W.
	Natural variant	315	1	I → T in strain: W.

Sequences

	Sequence		Length	Mass (Da)
	P76086 [UniParc]. Last modified February 1, 1997. Version 1. Checksum: 286C97DBC2564030	FASTA	316	35,295
	10 20 30 40 50 60 MSKLDTFIQH AVNAVPVSGT SLISSLYGDS LSHRGGEIWL GSLAALLEGL GFGERFVRTA 70 80 90 100 110 120 LFRLNKEGWL DVSRIGRRSF YSLSDKGLRL TRRAESKIYR AEQPAWDGKW LLLLSEGLDK 130 140 150 160 170 180 STLADVKKQL IWQGFGALAP SLMASPSQKL ADVQTLLHEA GVADNVICFE AQIPLALSRA 190 200 210 220 230 240 ALRARVEECW HLTEQNAMYE TFIQSFRPLV PLLKEAADEL TPERAFHIQL LLIHFYRRVV 250 260 270 280 290 300 LKDPLLPEEL LPAHWAGHTA RQLCINIYQR VAPAALAFVS EKGETSVGEL PAPGSLYFQR 310 FGGLNIEQEA LCQFIR

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Catabolism of phenylacetic acid in Escherichia coli. Characterization of a new aerobic hybrid pathway." Ferrandez A., Minambres B., Garcia B., Olivera E.R., Luengo J.M., Garcia J.L., Diaz E. J. Biol. Chem. 273:25974-25986(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION IN PHENYLACETATE CATABOLISM. Strain: W / ATCC 11105 / DSM 1900.
[2]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[3]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[4]	"Transcriptional regulation of the divergent paa catabolic operons for phenylacetic acid degradation in Escherichia coli." Ferrandez A., Garcia J.L., Diaz E. J. Biol. Chem. 275:12214-12222(2000) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION AS A TRANSCRIPTIONAL REPRESSOR, REGULATION BY PA-COA, CONSENSUS MOTIF.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X97452 Genomic DNA. Translation: CAA66101.1. U00096 Genomic DNA. Translation: AAC74481.1. AP009048 Genomic DNA. Translation: BAE76429.1.
PIR	B64891.
RefSeq	NP_415917.1. NC_000913.2. YP_489668.1. NC_007779.1.
3D structure databases
ProteinModelPortal	P76086.
SMR	P76086. Positions 11-99.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-10431N.
IntAct	P76086. 14 interactions.
STRING	511145.b1399.
Proteomic databases
PaxDb	P76086.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAC74481; AAC74481; b1399. BAE76429; BAE76429; BAE76429.
GeneID	12934020. 945966.
KEGG	ecj:Y75_p1376. eco:b1399.
PATRIC	32118084. VBIEscCol129921_1462.
Organism-specific databases
EchoBASE	EB3509.
EcoGene	EG13746. paaX.
Phylogenomic databases
eggNOG	COG3327.
HOGENOM	HOG000147151.
KO	K02616.
OMA	LPGDWEG.
ProtClustDB	CLSK891750.
Enzyme and pathway databases
BioCyc	EcoCyc:G6720-MONOMER. ECOL316407:JW1394-MONOMER.
UniPathway	UPA00930.
Gene expression databases
Genevestigator	P76086.
Family and domain databases
InterPro	IPR012906. PaaX-like_N. IPR013225. PaaX_C. IPR011965. PaaX_trns_reg. [Graphical view]
Pfam	PF07848. PaaX. 1 hit. PF08223. PaaX_C. 1 hit. [Graphical view]
PIRSF	PIRSF020623. PaaX. 1 hit.
TIGRFAMs	TIGR02277. PaaX_trns_reg. 1 hit.
ProtoNet	Search...

Entry information

Entry name

PAAX_ECOLI

Accession

Primary (citable) accession number: P76086
Secondary accession number(s): O53019, Q2MBC7

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1998
Last sequence update:	February 1, 1997
Last modified:	May 1, 2013
This is version 93 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program