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Protein

Phenylacetate-coenzyme A ligase

Gene

paaK

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the activation of phenylacetic acid (PA) to phenylacetyl-CoA (PA-CoA).2 Publications

Catalytic activityi

ATP + phenylacetate + CoA = AMP + diphosphate + phenylacetyl-CoA.

Pathwayi: phenylacetate degradation

This protein is involved in the pathway phenylacetate degradation, which is part of Aromatic compound metabolism.
View all proteins of this organism that are known to be involved in the pathway phenylacetate degradation and in Aromatic compound metabolism.

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • CoA-ligase activity Source: EcoliWiki
  • phenylacetate-CoA ligase activity Source: EcoliWiki

GO - Biological processi

  • phenylacetate catabolic process Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciEcoCyc:G6719-MONOMER.
ECOL316407:JW5218-MONOMER.
UniPathwayiUPA00930.

Names & Taxonomyi

Protein namesi
Recommended name:
Phenylacetate-coenzyme A ligase (EC:6.2.1.30)
Alternative name(s):
Phenylacetyl-CoA ligase
Short name:
PA-CoA ligase
Gene namesi
Name:paaK
Ordered Locus Names:b1398, JW5218
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG13745. paaK.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 437437Phenylacetate-coenzyme A ligasePRO_0000058164Add
BLAST

Proteomic databases

PaxDbiP76085.
PRIDEiP76085.

Expressioni

Inductioni

Activated by cAMP receptor protein (CRP), integration host factor (IHF) and by phenylacetyl-coenzyme A that prevents PaaX from binding its target sequences. Inhibited by PaaX.2 Publications

Interactioni

Subunit structurei

Monomer.By similarity

Protein-protein interaction databases

BioGridi4263013. 319 interactions.
DIPiDIP-10430N.
STRINGi511145.b1398.

Structurei

3D structure databases

ProteinModelPortaliP76085.
SMRiP76085. Positions 7-432.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the phenylacetyl-CoA ligase family.Curated

Phylogenomic databases

eggNOGiENOG4105DZS. Bacteria.
COG1541. LUCA.
HOGENOMiHOG000197614.
InParanoidiP76085.
KOiK01912.
OMAiGSHIWED.
PhylomeDBiP76085.

Family and domain databases

InterProiIPR028154. AMP-dep_Lig_C.
IPR000873. AMP-dep_Synth/Lig.
IPR011880. PA_CoA_ligase.
[Graphical view]
PfamiPF00501. AMP-binding. 1 hit.
PF14535. AMP-binding_C_2. 1 hit.
[Graphical view]
PIRSFiPIRSF006444. PaaK. 1 hit.
TIGRFAMsiTIGR02155. PA_CoA_ligase. 1 hit.

Sequencei

Sequence statusi: Complete.

P76085-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MITNTKLDPI ETASVDELQA LQTQRLKWTL KHAYENVPMY RRKFDAAGVH
60 70 80 90 100
PDDFRELSDL RKFPCTTKQD LRDNYPFDTF AVPMEQVVRI HASSGTTGKP
110 120 130 140 150
TVVGYTQNDI DNWANIVARS LRAAGGSPKD KIHVAYGYGL FTGGLGAHYG
160 170 180 190 200
AERLGATVIP MSGGQTEKQA QLIRDFQPDM IMVTPSYCLN LIEELERQLG
210 220 230 240 250
GDASGCSLRV GVFGAEPWTQ AMRKEIERRL GITALDIYGL SEVMGPGVAM
260 270 280 290 300
ECLETTDGPT IWEDHFYPEI VNPHDGTPLA DGEHGELLFT TLTKEALPVI
310 320 330 340 350
RYRTRDLTRL LPGTARTMRR MDRISGRSDD MLIIRGVNVF PSQLEEEIVK
360 370 380 390 400
FEHLSPHYQL EVNRRGHLDS LSVKVELKES SLTLTHEQRC QVCHQLRHRI
410 420 430
KSMVGISTDV MIVNCGSIPR SEGKACRVFD LRNIVGA
Length:437
Mass (Da):48,953
Last modified:February 1, 1997 - v1
Checksum:i3D49DB382B80F98A
GO

Sequence cautioni

The sequence CAA66100 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti127 – 1271S → T in strain: W.

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X97452 Genomic DNA. Translation: CAA66100.1. Different initiation.
U00096 Genomic DNA. Translation: AAC74480.1.
AP009048 Genomic DNA. Translation: BAE76428.1.
PIRiA64891.
RefSeqiNP_415916.1. NC_000913.3.
WP_000632280.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC74480; AAC74480; b1398.
BAE76428; BAE76428; BAE76428.
GeneIDi945963.
KEGGiecj:JW5218.
eco:b1398.
PATRICi32118082. VBIEscCol129921_1461.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X97452 Genomic DNA. Translation: CAA66100.1. Different initiation.
U00096 Genomic DNA. Translation: AAC74480.1.
AP009048 Genomic DNA. Translation: BAE76428.1.
PIRiA64891.
RefSeqiNP_415916.1. NC_000913.3.
WP_000632280.1. NZ_LN832404.1.

3D structure databases

ProteinModelPortaliP76085.
SMRiP76085. Positions 7-432.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4263013. 319 interactions.
DIPiDIP-10430N.
STRINGi511145.b1398.

Proteomic databases

PaxDbiP76085.
PRIDEiP76085.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC74480; AAC74480; b1398.
BAE76428; BAE76428; BAE76428.
GeneIDi945963.
KEGGiecj:JW5218.
eco:b1398.
PATRICi32118082. VBIEscCol129921_1461.

Organism-specific databases

EchoBASEiEB3508.
EcoGeneiEG13745. paaK.

Phylogenomic databases

eggNOGiENOG4105DZS. Bacteria.
COG1541. LUCA.
HOGENOMiHOG000197614.
InParanoidiP76085.
KOiK01912.
OMAiGSHIWED.
PhylomeDBiP76085.

Enzyme and pathway databases

UniPathwayiUPA00930.
BioCyciEcoCyc:G6719-MONOMER.
ECOL316407:JW5218-MONOMER.

Miscellaneous databases

PROiP76085.

Family and domain databases

InterProiIPR028154. AMP-dep_Lig_C.
IPR000873. AMP-dep_Synth/Lig.
IPR011880. PA_CoA_ligase.
[Graphical view]
PfamiPF00501. AMP-binding. 1 hit.
PF14535. AMP-binding_C_2. 1 hit.
[Graphical view]
PIRSFiPIRSF006444. PaaK. 1 hit.
TIGRFAMsiTIGR02155. PA_CoA_ligase. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiPAAK_ECOLI
AccessioniPrimary (citable) accession number: P76085
Secondary accession number(s): O53018, Q2MBC8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 27, 2001
Last sequence update: February 1, 1997
Last modified: September 7, 2016
This is version 114 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.