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Protein

Acyl-coenzyme A thioesterase PaaI

Gene

paaI

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Thioesterase with a preference for ring-hydroxylated phenylacetyl-CoA esters. Hydrolyzes 3,4-dihydroxyphenylacetyl-CoA, 3-hydroxyphenylacetyl-CoA and 4-hydroxyphenylacetyl-CoA. Inactive towards 4-hydroxybenzoyl-CoA and 4-hydroxyphenacyl-CoA.2 Publications

Kineticsi

  1. KM=16 µM for 3,4-dihydroxyphenylacetyl-CoA
  2. KM=21 µM for 3-hydroxyphenylacetyl-CoA
  3. KM=35 µM for 4-hydroxyphenylacetyl-CoA

    pH dependencei

    Optimum pH is 7.5-9.

    Pathwayi: phenylacetate degradation

    This protein is involved in the pathway phenylacetate degradation, which is part of Aromatic compound metabolism.
    View all proteins of this organism that are known to be involved in the pathway phenylacetate degradation and in Aromatic compound metabolism.

    GO - Molecular functioni

    • CoA hydrolase activity Source: EcoCyc

    GO - Biological processi

    Complete GO annotation...

    Keywords - Molecular functioni

    Hydrolase

    Enzyme and pathway databases

    BioCyciEcoCyc:G6717-MONOMER.
    ECOL316407:JW1391-MONOMER.
    MetaCyc:G6717-MONOMER.
    BRENDAi3.1.2.B5. 2026.
    UniPathwayiUPA00930.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Acyl-coenzyme A thioesterase PaaI (EC:3.1.2.-)
    Alternative name(s):
    Phenylacetic acid degradation protein PaaI
    Gene namesi
    Name:paaI
    Synonyms:ydbV
    Ordered Locus Names:b1396, JW1391
    OrganismiEscherichia coli (strain K12)
    Taxonomic identifieri83333 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
    Proteomesi
    • UP000000318 Componenti: Chromosome
    • UP000000625 Componenti: Chromosome

    Organism-specific databases

    EcoGeneiEG13743. paaI.

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi46 – 461N → A: Reduces activity 1000-fold. 1 Publication
    Mutagenesisi52 – 521H → A: Reduces activity 100-fold. 1 Publication
    Mutagenesisi61 – 611D → A: Loss of activity. 1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methionineiRemoved1 Publication
    Chaini2 – 140139Acyl-coenzyme A thioesterase PaaIPRO_0000156675Add
    BLAST

    Proteomic databases

    PaxDbiP76084.

    Expressioni

    Inductioni

    Activated by cAMP receptor protein (CRP), integration host factor (IHF) and by phenylacetyl-coenzyme A (PA-CoA) that prevents PaaX from binding its target sequences. Inhibited by PaaX.2 Publications

    Interactioni

    Subunit structurei

    Homotetramer.1 Publication

    Protein-protein interaction databases

    BioGridi4261735. 619 interactions.
    DIPiDIP-10428N.
    IntActiP76084. 2 interactions.
    STRINGi511145.b1396.

    Structurei

    Secondary structure

    1
    140
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi2 – 2221Combined sources
    Beta strandi25 – 306Combined sources
    Beta strandi33 – 397Combined sources
    Helixi42 – 443Combined sources
    Beta strandi49 – 513Combined sources
    Helixi53 – 6917Combined sources
    Turni70 – 723Combined sources
    Beta strandi76 – 849Combined sources
    Beta strandi93 – 10311Combined sources
    Beta strandi105 – 11511Combined sources
    Beta strandi121 – 13010Combined sources
    Beta strandi133 – 1353Combined sources

    3D structure databases

    Select the link destinations:
    PDBei
    RCSB PDBi
    PDBji
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1PSUX-ray2.20A/B1-140[»]
    2FS2X-ray2.00A/B2-140[»]
    ProteinModelPortaliP76084.
    SMRiP76084. Positions 1-137.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP76084.

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the thioesterase PaaI family.Curated

    Phylogenomic databases

    eggNOGiENOG4108ZJI. Bacteria.
    COG2050. LUCA.
    HOGENOMiHOG000066992.
    InParanoidiP76084.
    KOiK02614.
    OMAiGHGIAHG.
    PhylomeDBiP76084.

    Family and domain databases

    Gene3Di3.10.129.10. 1 hit.
    InterProiIPR029069. HotDog_dom.
    IPR011973. PaaD.
    IPR003736. PAAI_dom.
    IPR006683. Thioestr_dom.
    [Graphical view]
    PfamiPF03061. 4HBT. 1 hit.
    [Graphical view]
    SUPFAMiSSF54637. SSF54637. 1 hit.
    TIGRFAMsiTIGR02286. PaaD. 1 hit.
    TIGR00369. unchar_dom_1. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P76084-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MSHKAWQNAH AMYENDACAK ALGIDIISMD EGFAVVTMTV TAQMLNGHQS
    60 70 80 90 100
    CHGGQLFSLA DTAFAYACNS QGLAAVASAC TIDFLRPGFA GDTLTATAQV
    110 120 130 140
    RHQGKQTGVY DIEIVNQQQK TVALFRGKSH RIGGTITGEA
    Length:140
    Mass (Da):14,851
    Last modified:February 1, 1997 - v1
    Checksum:iA91DE9D2C8E8CF42
    GO

    Mass spectrometryi

    Molecular mass is 14720 Da from positions 2 - 140. Determined by ESI. 1 Publication

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    X97452 Genomic DNA. Translation: CAA66098.1.
    U00096 Genomic DNA. Translation: AAC74478.1.
    AP009048 Genomic DNA. Translation: BAE76427.1.
    PIRiG64890.
    RefSeqiNP_415914.1. NC_000913.3.
    WP_000018413.1. NZ_LN832404.1.

    Genome annotation databases

    EnsemblBacteriaiAAC74478; AAC74478; b1396.
    BAE76427; BAE76427; BAE76427.
    GeneIDi945265.
    KEGGiecj:JW1391.
    eco:b1396.
    PATRICi32118078. VBIEscCol129921_1459.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    X97452 Genomic DNA. Translation: CAA66098.1.
    U00096 Genomic DNA. Translation: AAC74478.1.
    AP009048 Genomic DNA. Translation: BAE76427.1.
    PIRiG64890.
    RefSeqiNP_415914.1. NC_000913.3.
    WP_000018413.1. NZ_LN832404.1.

    3D structure databases

    Select the link destinations:
    PDBei
    RCSB PDBi
    PDBji
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1PSUX-ray2.20A/B1-140[»]
    2FS2X-ray2.00A/B2-140[»]
    ProteinModelPortaliP76084.
    SMRiP76084. Positions 1-137.
    ModBaseiSearch...
    MobiDBiSearch...

    Protein-protein interaction databases

    BioGridi4261735. 619 interactions.
    DIPiDIP-10428N.
    IntActiP76084. 2 interactions.
    STRINGi511145.b1396.

    Proteomic databases

    PaxDbiP76084.

    Protocols and materials databases

    DNASUi945265.
    Structural Biology KnowledgebaseSearch...

    Genome annotation databases

    EnsemblBacteriaiAAC74478; AAC74478; b1396.
    BAE76427; BAE76427; BAE76427.
    GeneIDi945265.
    KEGGiecj:JW1391.
    eco:b1396.
    PATRICi32118078. VBIEscCol129921_1459.

    Organism-specific databases

    EchoBASEiEB3506.
    EcoGeneiEG13743. paaI.

    Phylogenomic databases

    eggNOGiENOG4108ZJI. Bacteria.
    COG2050. LUCA.
    HOGENOMiHOG000066992.
    InParanoidiP76084.
    KOiK02614.
    OMAiGHGIAHG.
    PhylomeDBiP76084.

    Enzyme and pathway databases

    UniPathwayiUPA00930.
    BioCyciEcoCyc:G6717-MONOMER.
    ECOL316407:JW1391-MONOMER.
    MetaCyc:G6717-MONOMER.
    BRENDAi3.1.2.B5. 2026.

    Miscellaneous databases

    EvolutionaryTraceiP76084.
    PROiP76084.

    Family and domain databases

    Gene3Di3.10.129.10. 1 hit.
    InterProiIPR029069. HotDog_dom.
    IPR011973. PaaD.
    IPR003736. PAAI_dom.
    IPR006683. Thioestr_dom.
    [Graphical view]
    PfamiPF03061. 4HBT. 1 hit.
    [Graphical view]
    SUPFAMiSSF54637. SSF54637. 1 hit.
    TIGRFAMsiTIGR02286. PaaD. 1 hit.
    TIGR00369. unchar_dom_1. 1 hit.
    ProtoNetiSearch...

    Entry informationi

    Entry nameiPAAI_ECOLI
    AccessioniPrimary (citable) accession number: P76084
    Secondary accession number(s): Q2MBC9
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 15, 1998
    Last sequence update: February 1, 1997
    Last modified: September 7, 2016
    This is version 122 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. Escherichia coli
      Escherichia coli (strain K12): entries and cross-references to EcoGene
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    4. SIMILARITY comments
      Index of protein domains and families
    5. Uncharacterized protein families (UPF)
      List of uncharacterized protein family (UPF) entries

    Similar proteinsi

    Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
    100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
    90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
    50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.