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Protein

1,2-phenylacetyl-CoA epoxidase, subunit E

Gene

paaE

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Component of 1,2-phenylacetyl-CoA epoxidase multicomponent enzyme system which catalyzes the reduction of phenylacetyl-CoA (PA-CoA) to form 1,2-epoxyphenylacetyl-CoA. The subunit E is a reductase with a preference for NADPH and FAD, capable of reducing cytochrome c.4 Publications

Cofactori

Protein has several cofactor binding sites:

Pathwayi: phenylacetate degradation

This protein is involved in the pathway phenylacetate degradation, which is part of Aromatic compound metabolism.
View all proteins of this organism that are known to be involved in the pathway phenylacetate degradation and in Aromatic compound metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi299Iron-sulfur (2Fe-2S)PROSITE-ProRule annotation1
Metal bindingi304Iron-sulfur (2Fe-2S)PROSITE-ProRule annotation1
Metal bindingi307Iron-sulfur (2Fe-2S)PROSITE-ProRule annotation1
Metal bindingi337Iron-sulfur (2Fe-2S)PROSITE-ProRule annotation1

GO - Molecular functioni

  • 2 iron, 2 sulfur cluster binding Source: EcoCyc
  • electron transfer activity Source: InterPro
  • flavin adenine dinucleotide binding Source: EcoCyc
  • metal ion binding Source: UniProtKB-KW

GO - Biological processi

  • phenylacetate catabolic process Source: UniProtKB

Keywordsi

Molecular functionOxidoreductase
Biological processElectron transport, Transport
Ligand2Fe-2S, FAD, Flavoprotein, Iron, Iron-sulfur, Metal-binding, NAD

Enzyme and pathway databases

BioCyciEcoCyc:G6713-MONOMER
MetaCyc:G6713-MONOMER
UniPathwayiUPA00930

Names & Taxonomyi

Protein namesi
Recommended name:
1,2-phenylacetyl-CoA epoxidase, subunit E (EC:1.-.-.-)
Alternative name(s):
1,2-phenylacetyl-CoA epoxidase, reductase subunit
1,2-phenylacetyl-CoA monooxygenase, subunit E
Gene namesi
Name:paaE
Synonyms:ydbR
Ordered Locus Names:b1392, JW1387
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG13739 paaE

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000581631 – 3561,2-phenylacetyl-CoA epoxidase, subunit EAdd BLAST356

Proteomic databases

PaxDbiP76081
PRIDEiP76081

Expressioni

Inductioni

Activated by cAMP receptor protein (CRP), integration host factor (IHF) and by phenylacetyl-coenzyme A (PA-CoA) that prevents PaaX from binding its target sequences. Inhibited by PaaX.2 Publications

Interactioni

Protein-protein interaction databases

BioGridi4260178, 205 interactors
IntActiP76081, 1 interactor
STRINGi316385.ECDH10B_1517

Structurei

3D structure databases

ProteinModelPortaliP76081
SMRiP76081
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini2 – 106FAD-binding FR-typePROSITE-ProRule annotationAdd BLAST105
Domaini262 – 3542Fe-2S ferredoxin-typePROSITE-ProRule annotationAdd BLAST93

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni112 – 228OxidoreductaseSequence analysisAdd BLAST117

Sequence similaritiesi

In the N-terminal section; belongs to the FAD-binding oxidoreductase type 6 family.Curated

Phylogenomic databases

eggNOGiENOG4105CJJ Bacteria
COG1018 LUCA
HOGENOMiHOG000141049
InParanoidiP76081
KOiK02613
OMAiWFLCGPF
PhylomeDBiP76081

Family and domain databases

CDDicd00207 fer2, 1 hit
Gene3Di3.10.20.30, 1 hit
InterProiView protein in InterPro
IPR036010 2Fe-2S_ferredoxin-like_sf
IPR001041 2Fe-2S_ferredoxin-type
IPR006058 2Fe2S_fd_BS
IPR012675 Beta-grasp_dom_sf
IPR017927 Fd_Rdtase_FAD-bd
IPR001709 Flavoprot_Pyr_Nucl_cyt_Rdtase
IPR008333 OxRdtase_FAD-bd_dom
IPR001433 OxRdtase_FAD/NAD-bd
IPR011884 PA_CoA_Oase5
IPR001221 Phe_hydroxylase
IPR017938 Riboflavin_synthase-like_b-brl
PfamiView protein in Pfam
PF00970 FAD_binding_6, 1 hit
PF00111 Fer2, 1 hit
PF00175 NAD_binding_1, 1 hit
PRINTSiPR00371 FPNCR
PR00410 PHEHYDRXLASE
SUPFAMiSSF54292 SSF54292, 1 hit
SSF63380 SSF63380, 1 hit
TIGRFAMsiTIGR02160 PA_CoA_Oxy5, 1 hit
PROSITEiView protein in PROSITE
PS00197 2FE2S_FER_1, 1 hit
PS51085 2FE2S_FER_2, 1 hit
PS51384 FAD_FR, 1 hit

Sequencei

Sequence statusi: Complete.

P76081-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTTFHSLTVA KVESETRDAV TITFAVPQPL QEAYRFRPGQ HLTLKASFDG
60 70 80 90 100
EELRRCYSIC RSYLPGEISV AVKAIEGGRF SRYAREHIRQ GMTLEVMVPQ
110 120 130 140 150
GHFGYQPQAE RQGRYLAIAA GSGITPMLAI IATTLQTEPE SQFTLIYGNR
160 170 180 190 200
TSQSMMFRQA LADLKDKYPQ RLQLLCIFSQ ETLDSDLLHG RIDGEKLQSL
210 220 230 240 250
GASLINFRLY DEAFICGPAA MMDDAETALK ALGMPDKTIH LERFNTPGTR
260 270 280 290 300
VKRSVNVQSD GQKVTVRQDG RDREIVLNAD DESILDAALR QGADLPYACK
310 320 330 340 350
GGVCATCKCK VLRGKVAMET NYSLEPDELA AGYVLSCQAL PLTSDVVVDF

DAKGMA
Length:356
Mass (Da):39,320
Last modified:February 1, 1997 - v1
Checksum:iD719C1CA81DA5FFA
GO

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural varianti14S → P in strain: W. 1
Natural varianti169P → S in strain: W. 1
Natural varianti224 – 227DAET → ETEA in strain: W. 4

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X97452 Genomic DNA Translation: CAA66094.1
U00096 Genomic DNA Translation: AAC74474.1
AP009048 Genomic DNA Translation: BAA14998.2
PIRiC64890
RefSeqiNP_415910.1, NC_000913.3
WP_000206388.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaiAAC74474; AAC74474; b1392
BAA14998; BAA14998; BAA14998
GeneIDi945962
KEGGiecj:JW1387
eco:b1392
PATRICifig|1411691.4.peg.879

Similar proteinsi

Entry informationi

Entry nameiPAAE_ECOLI
AccessioniPrimary (citable) accession number: P76081
Secondary accession number(s): O53013, P77233
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 27, 2001
Last sequence update: February 1, 1997
Last modified: March 28, 2018
This is version 144 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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