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P76080 (PAAD_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 86. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Putative 1,2-phenylacetyl-CoA epoxidase, subunit D
Alternative name(s):
1,2-phenylacetyl-CoA monooxygenase, subunit D
Gene names
Name:paaD
Synonyms:ydbQ
Ordered Locus Names:b1391, JW5217
OrganismEscherichia coli (strain K12) [Reference proteome] [HAMAP]
Taxonomic identifier83333 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia

Protein attributes

Sequence length165 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Possible component of 1,2-phenylacetyl-CoA epoxidase multicomponent enzyme system which catalyzes the reduction of phenylacetyl-CoA (PA-CoA) to form 1,2-epoxyphenylacetyl-CoA. The subunit D may have a function related to the maturation of the monooxygenase complex, rather than direct involvement in catalysis. PaaD could assist either in maturation of PaaE or PaaA. Ref.1 Ref.5

Pathway

Aromatic compound metabolism; phenylacetate degradation.

Subunit structure

Monomer. Ref.6

Induction

Activated by cAMP receptor protein (CRP), integration host factor (IHF) and by phenylacetyl-coenzyme A (PA-CoA) that prevents PaaX from binding its target sequences. Inhibited by PaaX. Ref.1 Ref.4

Miscellaneous

PaaD component is not essential for the reaction in vitro.

Sequence caution

The sequence CAA66093.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.

Ontologies

Keywords
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processphenylacetate catabolic process

Inferred from mutant phenotype Ref.1. Source: UniProtKB

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 165165Putative 1,2-phenylacetyl-CoA epoxidase, subunit D
PRO_0000058162

Natural variations

Natural variant471G → E in strain: W.
Natural variant761N → H in strain: W.
Natural variant1061E → Q in strain: W.

Sequences

Sequence LengthMass (Da)Tools
P76080 [UniParc].

Last modified November 24, 2009. Version 2.
Checksum: 67AE8F04C8F0045B

FASTA16518,324
        10         20         30         40         50         60 
MQRLATIAPP QVHEIWALLS QIPDPEIPVL TITDLGMVRN VTQMGEGWVI GFTPTYSGCP 

        70         80         90        100        110        120 
ATEHLIGAIR EAMTTNGFTP VQVVLQLDPA WTTDWMTPDA RERLREYGIS PPAGHSCHAH 

       130        140        150        160 
LPPEVRCPRC ASVHTTLISE FGSTACKALY RCDSCREPFD YFKCI 

« Hide

References

« Hide 'large scale' references
[1]"Catabolism of phenylacetic acid in Escherichia coli. Characterization of a new aerobic hybrid pathway."
Ferrandez A., Minambres B., Garcia B., Olivera E.R., Luengo J.M., Garcia J.L., Diaz E.
J. Biol. Chem. 273:25974-25986(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION IN PHENYLACETATE CATABOLISM, INDUCTION.
Strain: W / ATCC 11105 / DSM 1900.
[2]"The complete genome sequence of Escherichia coli K-12."
Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y.
Science 277:1453-1462(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / MG1655 / ATCC 47076.
[3]"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[4]"Transcriptional regulation of the divergent paa catabolic operons for phenylacetic acid degradation in Escherichia coli."
Ferrandez A., Garcia J.L., Diaz E.
J. Biol. Chem. 275:12214-12222(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: TRANSCRIPTIONAL REGULATION.
[5]"Genetic characterization of the phenylacetyl-coenzyme A oxygenase from the aerobic phenylacetic acid degradation pathway of Escherichia coli."
Fernandez C., Ferrandez A., Minambres B., Diaz E., Garcia J.L.
Appl. Environ. Microbiol. 72:7422-7426(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION AS A POSSIBLE MONOOXYGENASE COMPONENT.
[6]"Structural and functional studies of the Escherichia coli phenylacetyl-CoA monooxygenase complex."
Grishin A.M., Ajamian E., Tao L., Zhang L., Menard R., Cygler M.
J. Biol. Chem. 286:10735-10743(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBUNIT.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X97452 Genomic DNA. Translation: CAA66093.1. Different initiation.
U00096 Genomic DNA. Translation: AAC74473.4.
AP009048 Genomic DNA. Translation: BAE76426.1.
PIRB64890.
RefSeqNP_415909.4. NC_000913.3.
YP_489660.1. NC_007779.1.

3D structure databases

ProteinModelPortalP76080.
SMRP76080. Positions 12-104.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

IntActP76080. 4 interactions.
STRING511145.b1391.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAC74473; AAC74473; b1391.
BAE76426; BAE76426; BAE76426.
GeneID12933892.
945959.
KEGGecj:Y75_p1368.
eco:b1391.
PATRIC32118068. VBIEscCol129921_1454.

Organism-specific databases

EchoBASEEB3501.
EcoGeneEG13738. paaD.

Phylogenomic databases

eggNOGCOG2151.
HOGENOMHOG000249723.
KOK02612.
OrthoDBEOG664CH5.
PhylomeDBP76080.
ProtClustDBCLSK950271.

Enzyme and pathway databases

BioCycEcoCyc:G6712-MONOMER.
ECOL316407:JW5217-MONOMER.
UniPathwayUPA00930.

Gene expression databases

GenevestigatorP76080.

Family and domain databases

InterProIPR002744. DUF59.
IPR011883. PA_CoA_Oase4.
[Graphical view]
PfamPF01883. DUF59. 1 hit.
[Graphical view]
TIGRFAMsTIGR02159. PA_CoA_Oxy4. 1 hit.
ProtoNetSearch...

Other

PROP76080.

Entry information

Entry namePAAD_ECOLI
AccessionPrimary (citable) accession number: P76080
Secondary accession number(s): O53012, Q2MBD0
Entry history
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: November 24, 2009
Last modified: April 16, 2014
This is version 86 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

Escherichia coli

Escherichia coli (strain K12): entries and cross-references to EcoGene