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P76080

- PAAD_ECOLI

UniProt

P76080 - PAAD_ECOLI

Protein

Putative 1,2-phenylacetyl-CoA epoxidase, subunit D

Gene

paaD

Organism
Escherichia coli (strain K12)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 88 (01 Oct 2014)
      Sequence version 2 (24 Nov 2009)
      Previous versions | rss
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    Functioni

    Possible component of 1,2-phenylacetyl-CoA epoxidase multicomponent enzyme system which catalyzes the reduction of phenylacetyl-CoA (PA-CoA) to form 1,2-epoxyphenylacetyl-CoA. The subunit D may have a function related to the maturation of the monooxygenase complex, rather than direct involvement in catalysis. PaaD could assist either in maturation of PaaE or PaaA.2 Publications

    Pathwayi

    GO - Biological processi

    1. phenylacetate catabolic process Source: UniProtKB

    Enzyme and pathway databases

    BioCyciEcoCyc:G6712-MONOMER.
    ECOL316407:JW5217-MONOMER.
    UniPathwayiUPA00930.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Putative 1,2-phenylacetyl-CoA epoxidase, subunit D
    Alternative name(s):
    1,2-phenylacetyl-CoA monooxygenase, subunit D
    Gene namesi
    Name:paaD
    Synonyms:ydbQ
    Ordered Locus Names:b1391, JW5217
    OrganismiEscherichia coli (strain K12)
    Taxonomic identifieri83333 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
    ProteomesiUP000000318: Chromosome, UP000000625: Chromosome

    Organism-specific databases

    EcoGeneiEG13738. paaD.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 165165Putative 1,2-phenylacetyl-CoA epoxidase, subunit DPRO_0000058162Add
    BLAST

    Expressioni

    Inductioni

    Activated by cAMP receptor protein (CRP), integration host factor (IHF) and by phenylacetyl-coenzyme A (PA-CoA) that prevents PaaX from binding its target sequences. Inhibited by PaaX.2 Publications

    Gene expression databases

    GenevestigatoriP76080.

    Interactioni

    Subunit structurei

    Monomer.1 Publication

    Protein-protein interaction databases

    IntActiP76080. 4 interactions.
    STRINGi511145.b1391.

    Structurei

    3D structure databases

    ProteinModelPortaliP76080.
    SMRiP76080. Positions 12-104.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Phylogenomic databases

    eggNOGiCOG2151.
    HOGENOMiHOG000249723.
    KOiK02612.
    OrthoDBiEOG664CH5.
    PhylomeDBiP76080.

    Family and domain databases

    InterProiIPR002744. DUF59.
    IPR011883. PA_CoA_Oase4.
    [Graphical view]
    PfamiPF01883. DUF59. 1 hit.
    [Graphical view]
    TIGRFAMsiTIGR02159. PA_CoA_Oxy4. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    P76080-1 [UniParc]FASTAAdd to Basket

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    MQRLATIAPP QVHEIWALLS QIPDPEIPVL TITDLGMVRN VTQMGEGWVI    50
    GFTPTYSGCP ATEHLIGAIR EAMTTNGFTP VQVVLQLDPA WTTDWMTPDA 100
    RERLREYGIS PPAGHSCHAH LPPEVRCPRC ASVHTTLISE FGSTACKALY 150
    RCDSCREPFD YFKCI 165
    Length:165
    Mass (Da):18,324
    Last modified:November 24, 2009 - v2
    Checksum:i67AE8F04C8F0045B
    GO

    Sequence cautioni

    The sequence CAA66093.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti47 – 471G → E in strain: W.
    Natural varianti76 – 761N → H in strain: W.
    Natural varianti106 – 1061E → Q in strain: W.

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X97452 Genomic DNA. Translation: CAA66093.1. Different initiation.
    U00096 Genomic DNA. Translation: AAC74473.4.
    AP009048 Genomic DNA. Translation: BAE76426.1.
    PIRiB64890.
    RefSeqiNP_415909.4. NC_000913.3.
    YP_489660.1. NC_007779.1.

    Genome annotation databases

    EnsemblBacteriaiAAC74473; AAC74473; b1391.
    BAE76426; BAE76426; BAE76426.
    GeneIDi12933892.
    945959.
    KEGGiecj:Y75_p1368.
    eco:b1391.
    PATRICi32118068. VBIEscCol129921_1454.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X97452 Genomic DNA. Translation: CAA66093.1 . Different initiation.
    U00096 Genomic DNA. Translation: AAC74473.4 .
    AP009048 Genomic DNA. Translation: BAE76426.1 .
    PIRi B64890.
    RefSeqi NP_415909.4. NC_000913.3.
    YP_489660.1. NC_007779.1.

    3D structure databases

    ProteinModelPortali P76080.
    SMRi P76080. Positions 12-104.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi P76080. 4 interactions.
    STRINGi 511145.b1391.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAC74473 ; AAC74473 ; b1391 .
    BAE76426 ; BAE76426 ; BAE76426 .
    GeneIDi 12933892.
    945959.
    KEGGi ecj:Y75_p1368.
    eco:b1391.
    PATRICi 32118068. VBIEscCol129921_1454.

    Organism-specific databases

    EchoBASEi EB3501.
    EcoGenei EG13738. paaD.

    Phylogenomic databases

    eggNOGi COG2151.
    HOGENOMi HOG000249723.
    KOi K02612.
    OrthoDBi EOG664CH5.
    PhylomeDBi P76080.

    Enzyme and pathway databases

    UniPathwayi UPA00930 .
    BioCyci EcoCyc:G6712-MONOMER.
    ECOL316407:JW5217-MONOMER.

    Miscellaneous databases

    PROi P76080.

    Gene expression databases

    Genevestigatori P76080.

    Family and domain databases

    InterProi IPR002744. DUF59.
    IPR011883. PA_CoA_Oase4.
    [Graphical view ]
    Pfami PF01883. DUF59. 1 hit.
    [Graphical view ]
    TIGRFAMsi TIGR02159. PA_CoA_Oxy4. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Catabolism of phenylacetic acid in Escherichia coli. Characterization of a new aerobic hybrid pathway."
      Ferrandez A., Minambres B., Garcia B., Olivera E.R., Luengo J.M., Garcia J.L., Diaz E.
      J. Biol. Chem. 273:25974-25986(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION IN PHENYLACETATE CATABOLISM, INDUCTION.
      Strain: W / ATCC 11105 / DSM 1900.
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / MG1655 / ATCC 47076.
    3. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
      Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
      Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
    4. "Transcriptional regulation of the divergent paa catabolic operons for phenylacetic acid degradation in Escherichia coli."
      Ferrandez A., Garcia J.L., Diaz E.
      J. Biol. Chem. 275:12214-12222(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: TRANSCRIPTIONAL REGULATION.
    5. "Genetic characterization of the phenylacetyl-coenzyme A oxygenase from the aerobic phenylacetic acid degradation pathway of Escherichia coli."
      Fernandez C., Ferrandez A., Minambres B., Diaz E., Garcia J.L.
      Appl. Environ. Microbiol. 72:7422-7426(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION AS A POSSIBLE MONOOXYGENASE COMPONENT.
    6. "Structural and functional studies of the Escherichia coli phenylacetyl-CoA monooxygenase complex."
      Grishin A.M., Ajamian E., Tao L., Zhang L., Menard R., Cygler M.
      J. Biol. Chem. 286:10735-10743(2011) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBUNIT.

    Entry informationi

    Entry nameiPAAD_ECOLI
    AccessioniPrimary (citable) accession number: P76080
    Secondary accession number(s): O53012, Q2MBD0
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 15, 1998
    Last sequence update: November 24, 2009
    Last modified: October 1, 2014
    This is version 88 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    PaaD component is not essential for the reaction in vitro.

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Escherichia coli
      Escherichia coli (strain K12): entries and cross-references to EcoGene
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways

    External Data

    Dasty 3