Reviewed, UniProtKB/Swiss-Prot P76079 (PAAC_ECOLI)

Names and origin

Protein names

Recommended name:
    Phenylacetic acid degradation protein paaC

Gene names

Name:	paaC
Synonyms:	ydbP
Ordered Locus Names:	b1390, JW1385

Organism

Escherichia coli (strain K12) [Complete proteome] [HAMAP]

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	248 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	May be part of a multicomponent oxygenase involved in phenylacetyl-CoA hydroxylation.
Pathway	Aromatic compound metabolism; phenylacetate degradation.
Induction	Activated by cAMP receptor protein (CRP) and integration host factor (IHF). Inhibited by paaX. Ref.4

Ontologies

Keywords
Technical term	3D-structure Complete proteome
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: InterPro
Molecular function	oxidoreductase activity Inferred from electronic annotation. Source: InterPro transition metal ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 248

248

Phenylacetic acid degradation protein paaC

PRO_0000058161

Natural variations

Natural variant

160

1

N → D in strain: W.

Secondary structure

1

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

248

Helix Strand Turn

Details...

Helix

2 – 23

22

Turn

24 – 28

5

Beta strand

29 – 31

3

Helix

32 – 60

29

Helix

65 – 70

6

Helix

74 – 76

3

Helix

81 – 84

4

Helix

90 – 111

22

Helix

117 – 145

29

Helix

149 – 162

14

Helix

163 – 165

3

Helix

167 – 170

4

Helix

174 – 181

8

Helix

188 – 191

4

Helix

192 – 205

14

Helix

220 – 222

3

Helix

229 – 242

14

Sequences

	Sequence		Length	Mass (Da)
	P76079-1 [UniParc]. Last modified February 1, 1997. Version 1. Checksum: 024AD161DF8E4380	FASTA	248	27,877
	10 20 30 40 50 60 MNQLTAYTLR LGDNCLVLSQ RLGEWCGHAP ELEIDLALAN IGLDLLGQAR NFLSYAAELA 70 80 90 100 110 120 GEGDEDTLAF TRDERQFSNL LLVEQPNGNF ADTIARQYFI DAWHVALFTR LMESRDPQLA 130 140 150 160 170 180 AISAKAIKEA RYHLRFSRGW LERLGNGTDV SGQKMQQAIN KLWRFTAELF DADEIDIALS 190 200 210 220 230 240 EEGIAVDPRT LRAAWEAEVF AGINEATLNV PQEQAYRTGG KKGLHTEHLG PMLAEMQYLQ RVLPGQQW

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Catabolism of phenylacetic acid in Escherichia coli. Characterization of a new aerobic hybrid pathway." Ferrandez A., Minambres B., Garcia B., Olivera E.R., Luengo J.M., Garcia J.L., Diaz E. J. Biol. Chem. 273:25974-25986(1998) [PubMed: 9748275] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: W / ATCC 11105 / DSM 1900.
[2]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[3]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed: 16738553] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[4]	"Transcriptional regulation of the divergent paa catabolic operons for phenylacetic acid degradation in Escherichia coli." Ferrandez A., Garcia J.L., Diaz E. J. Biol. Chem. 275:12214-12222(2000) [PubMed: 10766858] [Abstract] Cited for: TRANSCRIPTIONAL REGULATION.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

X97452 Genomic DNA. Translation: CAA66092.1.
U00096 Genomic DNA. Translation: AAC74472.1.
AP009048 Genomic DNA. Translation: BAE76425.1.

PIR

A64890.

RefSeq

AP_002017.1.
NP_415908.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1OTK	X-ray	2.00	A/B	2-248	[»]

ModBase

Search...

Protein-protein interaction databases

STRING

P76079.

Genome annotation databases

GeneID

945956.

GenomeReviews

Gene locus JW1385 in contig AP009048_GR.
Gene locus b1390 in contig U00096_GR.

KEGG

ecj:JW1385.
eco:b1390.

Organism-specific databases

EchoBASE

EB3500.

EcoGene

EG13737. paaC.

CMR

Search...

Phylogenomic databases

eggNOG

COG3396.

HOGENOM

HBG348178.

OMA

LAEMQFL.

Enzyme and pathway databases

BioCyc

EcoCyc:G6711-MONOMER.
ECOL168927:B1390-MONOMER.

Gene expression databases

Genevestigator

P76079.

Family and domain databases

InterPro

IPR009078. Ferritin/RR-like.
IPR012347. Ferritin_rel.
IPR011882. PA_CoA_Oase3.
IPR007814. PaaA_PaaC.
[Graphical view]

Gene3D

G3DSA:1.20.1260.10. Ferritin_rel. 1 hit.

Pfam

PF05138. PaaA_PaaC. 1 hit.
[Graphical view]

PIRSF

PIRSF037834. PA_CoA_Oase3. 1 hit.

TIGRFAMs

TIGR02158. PA_CoA_Oxy3. 1 hit.

ProtoNet

Search...

Entry information

Entry name

PAAC_ECOLI

Accession

Primary (citable) accession number: P76079
Secondary accession number(s): O53011, Q2MBD1

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1998
Last sequence update:	February 1, 1997
Last modified:	February 9, 2010
This is version 72 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)