Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

1,2-phenylacetyl-CoA epoxidase, subunit A

Gene

paaA

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Component of 1,2-phenylacetyl-CoA epoxidase multicomponent enzyme system which catalyzes the reduction of phenylacetyl-CoA (PA-CoA) to form 1,2-epoxyphenylacetyl-CoA. The subunit A is the catalytic subunit involved in the incorporation of one atom of molecular oxygen into phenylacetyl-CoA.4 Publications

Catalytic activityi

Phenylacetyl-CoA + NADPH + O2 = 2-(1,2-epoxy-1,2-dihydrophenyl)acetyl-CoA + NADP+ + H2O.1 Publication

Cofactori

Fe cationCurated

Pathwayi: phenylacetate degradation

This protein is involved in the pathway phenylacetate degradation, which is part of Aromatic compound metabolism.
View all proteins of this organism that are known to be involved in the pathway phenylacetate degradation and in Aromatic compound metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei33Substrate1 Publication1
Binding sitei37Substrate1 Publication1
Binding sitei132Substrate1 Publication1
Binding sitei193Substrate1 Publication1
Binding sitei214Substrate1 Publication1
Binding sitei218Substrate1 Publication1

GO - Molecular functioni

GO - Biological processi

  • phenylacetate catabolic process Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Ligandi

NADP

Enzyme and pathway databases

BioCyciEcoCyc:G6709-MONOMER.
ECOL316407:JW1383-MONOMER.
MetaCyc:G6709-MONOMER.
UniPathwayiUPA00930.

Names & Taxonomyi

Protein namesi
Recommended name:
1,2-phenylacetyl-CoA epoxidase, subunit A (EC:1.14.13.149)
Alternative name(s):
1,2-phenylacetyl-CoA epoxidase, catalytic subunit alpha
1,2-phenylacetyl-CoA monooxygenase, subunit A
Gene namesi
Name:paaA
Synonyms:ydbO
Ordered Locus Names:b1388, JW1383
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG13736. paaA.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000581591 – 3091,2-phenylacetyl-CoA epoxidase, subunit AAdd BLAST309

Proteomic databases

PaxDbiP76077.
PRIDEiP76077.

Expressioni

Inductioni

Activated by cAMP receptor protein (CRP), integration host factor (IHF) and by phenylacetyl-coenzyme A (PA-CoA) that prevents PaaX from binding its target sequences. Inhibited by PaaX.2 Publications

Interactioni

Subunit structurei

Forms a stable heterotetramer (dimer of heterodimers) with PaaC.2 Publications

Protein-protein interaction databases

BioGridi4261392. 123 interactors.
IntActiP76077. 1 interactor.
STRINGi511145.b1388.

Structurei

Secondary structure

1309
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi2 – 12Combined sources11
Helixi25 – 44Combined sources20
Helixi46 – 50Combined sources5
Helixi51 – 55Combined sources5
Helixi59 – 84Combined sources26
Turni85 – 87Combined sources3
Helixi90 – 98Combined sources9
Helixi106 – 109Combined sources4
Helixi115 – 134Combined sources20
Turni135 – 138Combined sources4
Helixi142 – 170Combined sources29
Helixi174 – 193Combined sources20
Helixi199 – 201Combined sources3
Helixi205 – 210Combined sources6
Helixi218 – 235Combined sources18
Beta strandi246 – 248Combined sources3
Turni249 – 252Combined sources4
Beta strandi253 – 255Combined sources3
Helixi261 – 268Combined sources8
Turni269 – 271Combined sources3
Beta strandi272 – 274Combined sources3
Helixi275 – 288Combined sources14
Helixi290 – 301Combined sources12

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3PVRX-ray2.10A2-309[»]
3PVTX-ray2.03A2-309[»]
3PVYX-ray2.15A2-309[»]
3PW1X-ray2.25A2-309[»]
3PW8X-ray2.97C/D2-309[»]
3PWQX-ray2.65C/D/F/H2-309[»]
4II4X-ray2.80A2-309[»]
ProteinModelPortaliP76077.
SMRiP76077.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP76077.

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni103 – 106Substrate binding1 Publication4
Regioni202 – 204Substrate binding1 Publication3

Phylogenomic databases

eggNOGiENOG4105CRS. Bacteria.
COG3396. LUCA.
HOGENOMiHOG000249745.
InParanoidiP76077.
KOiK02609.
OMAiQPEANWI.
PhylomeDBiP76077.

Family and domain databases

Gene3Di1.20.1260.10. 1 hit.
InterProiIPR009078. Ferritin-like_SF.
IPR012347. Ferritin-rel.
IPR011881. PaaA.
IPR007814. PaaA_PaaC.
[Graphical view]
PfamiPF05138. PaaA_PaaC. 1 hit.
[Graphical view]
SUPFAMiSSF47240. SSF47240. 1 hit.
TIGRFAMsiTIGR02156. PA_CoA_Oxy1. 1 hit.

Sequencei

Sequence statusi: Complete.

P76077-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTQEERFEQR IAQETAIEPQ DWMPDAYRKT LIRQIGQHAH SEIVGMLPEG
60 70 80 90 100
NWITRAPTLR RKAILLAKVQ DEAGHGLYLY SAAETLGCAR EDIYQKMLDG
110 120 130 140 150
RMKYSSIFNY PTLSWADIGV IGWLVDGAAI VNQVALCRTS YGPYARAMVK
160 170 180 190 200
ICKEESFHQR QGFEACMALA QGSEAQKQML QDAINRFWWP ALMMFGPNDD
210 220 230 240 250
NSPNSARSLT WKIKRFTNDE LRQRFVDNTV PQVEMLGMTV PDPDLHFDTE
260 270 280 290 300
SGHYRFGEID WQEFNEVING RGICNQERLD AKRKAWEEGT WVREAALAHA

QKQHARKVA
Length:309
Mass (Da):35,499
Last modified:February 1, 1997 - v1
Checksum:i83AEC6D7AC084073
GO

Sequence cautioni

The sequence CAA66090 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural varianti210T → A in strain: W. 1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X97452 Genomic DNA. Translation: CAA66090.1. Different initiation.
U00096 Genomic DNA. Translation: AAC74470.1.
AP009048 Genomic DNA. Translation: BAE76423.1.
PIRiG64889.
RefSeqiNP_415906.1. NC_000913.3.
WP_000191077.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC74470; AAC74470; b1388.
BAE76423; BAE76423; BAE76423.
GeneIDi945833.
KEGGiecj:JW1383.
eco:b1388.
PATRICi32118062. VBIEscCol129921_1451.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X97452 Genomic DNA. Translation: CAA66090.1. Different initiation.
U00096 Genomic DNA. Translation: AAC74470.1.
AP009048 Genomic DNA. Translation: BAE76423.1.
PIRiG64889.
RefSeqiNP_415906.1. NC_000913.3.
WP_000191077.1. NZ_LN832404.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3PVRX-ray2.10A2-309[»]
3PVTX-ray2.03A2-309[»]
3PVYX-ray2.15A2-309[»]
3PW1X-ray2.25A2-309[»]
3PW8X-ray2.97C/D2-309[»]
3PWQX-ray2.65C/D/F/H2-309[»]
4II4X-ray2.80A2-309[»]
ProteinModelPortaliP76077.
SMRiP76077.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4261392. 123 interactors.
IntActiP76077. 1 interactor.
STRINGi511145.b1388.

Proteomic databases

PaxDbiP76077.
PRIDEiP76077.

Protocols and materials databases

DNASUi945833.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC74470; AAC74470; b1388.
BAE76423; BAE76423; BAE76423.
GeneIDi945833.
KEGGiecj:JW1383.
eco:b1388.
PATRICi32118062. VBIEscCol129921_1451.

Organism-specific databases

EchoBASEiEB3499.
EcoGeneiEG13736. paaA.

Phylogenomic databases

eggNOGiENOG4105CRS. Bacteria.
COG3396. LUCA.
HOGENOMiHOG000249745.
InParanoidiP76077.
KOiK02609.
OMAiQPEANWI.
PhylomeDBiP76077.

Enzyme and pathway databases

UniPathwayiUPA00930.
BioCyciEcoCyc:G6709-MONOMER.
ECOL316407:JW1383-MONOMER.
MetaCyc:G6709-MONOMER.

Miscellaneous databases

EvolutionaryTraceiP76077.
PROiP76077.

Family and domain databases

Gene3Di1.20.1260.10. 1 hit.
InterProiIPR009078. Ferritin-like_SF.
IPR012347. Ferritin-rel.
IPR011881. PaaA.
IPR007814. PaaA_PaaC.
[Graphical view]
PfamiPF05138. PaaA_PaaC. 1 hit.
[Graphical view]
SUPFAMiSSF47240. SSF47240. 1 hit.
TIGRFAMsiTIGR02156. PA_CoA_Oxy1. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiPAAA_ECOLI
AccessioniPrimary (citable) accession number: P76077
Secondary accession number(s): O53010, Q2MBD3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: February 1, 1997
Last modified: November 2, 2016
This is version 113 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.