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Protein

1,2-phenylacetyl-CoA epoxidase, subunit A

Gene

paaA

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Component of 1,2-phenylacetyl-CoA epoxidase multicomponent enzyme system which catalyzes the reduction of phenylacetyl-CoA (PA-CoA) to form 1,2-epoxyphenylacetyl-CoA. The subunit A is the catalytic subunit involved in the incorporation of one atom of molecular oxygen into phenylacetyl-CoA.4 Publications

Catalytic activityi

Phenylacetyl-CoA + NADPH + O2 = 2-(1,2-epoxy-1,2-dihydrophenyl)acetyl-CoA + NADP+ + H2O.1 Publication

Cofactori

Fe cationCurated

Pathwayi: phenylacetate degradation

This protein is involved in the pathway phenylacetate degradation, which is part of Aromatic compound metabolism.
View all proteins of this organism that are known to be involved in the pathway phenylacetate degradation and in Aromatic compound metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei33Substrate1 Publication1
Binding sitei37Substrate1 Publication1
Binding sitei132Substrate1 Publication1
Binding sitei193Substrate1 Publication1
Binding sitei214Substrate1 Publication1
Binding sitei218Substrate1 Publication1

GO - Molecular functioni

GO - Biological processi

  • phenylacetate catabolic process Source: UniProtKB

Keywordsi

Molecular functionOxidoreductase
LigandNADP

Enzyme and pathway databases

BioCyciEcoCyc:G6709-MONOMER
MetaCyc:G6709-MONOMER
UniPathwayiUPA00930

Names & Taxonomyi

Protein namesi
Recommended name:
1,2-phenylacetyl-CoA epoxidase, subunit A (EC:1.14.13.149)
Alternative name(s):
1,2-phenylacetyl-CoA epoxidase, catalytic subunit alpha
1,2-phenylacetyl-CoA monooxygenase, subunit A
Gene namesi
Name:paaA
Synonyms:ydbO
Ordered Locus Names:b1388, JW1383
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG13736 paaA

Subcellular locationi

GO - Cellular componenti

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000581591 – 3091,2-phenylacetyl-CoA epoxidase, subunit AAdd BLAST309

Proteomic databases

PaxDbiP76077
PRIDEiP76077

Expressioni

Inductioni

Activated by cAMP receptor protein (CRP), integration host factor (IHF) and by phenylacetyl-coenzyme A (PA-CoA) that prevents PaaX from binding its target sequences. Inhibited by PaaX.2 Publications

Interactioni

Subunit structurei

Forms a stable heterotetramer (dimer of heterodimers) with PaaC.2 Publications

Protein-protein interaction databases

BioGridi4261392, 123 interactors
IntActiP76077, 1 interactor
STRINGi316385.ECDH10B_1513

Structurei

Secondary structure

1309
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi2 – 12Combined sources11
Helixi25 – 44Combined sources20
Helixi46 – 50Combined sources5
Helixi51 – 55Combined sources5
Helixi59 – 84Combined sources26
Turni85 – 87Combined sources3
Helixi90 – 98Combined sources9
Helixi106 – 109Combined sources4
Helixi115 – 134Combined sources20
Turni135 – 138Combined sources4
Helixi142 – 170Combined sources29
Helixi174 – 193Combined sources20
Helixi199 – 201Combined sources3
Helixi205 – 210Combined sources6
Helixi218 – 235Combined sources18
Beta strandi246 – 248Combined sources3
Turni249 – 252Combined sources4
Beta strandi253 – 255Combined sources3
Helixi261 – 268Combined sources8
Turni269 – 271Combined sources3
Beta strandi272 – 274Combined sources3
Helixi275 – 288Combined sources14
Helixi290 – 301Combined sources12

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3PVRX-ray2.10A2-309[»]
3PVTX-ray2.03A2-309[»]
3PVYX-ray2.15A2-309[»]
3PW1X-ray2.25A2-309[»]
3PW8X-ray2.97C/D2-309[»]
3PWQX-ray2.65C/D/F/H2-309[»]
4II4X-ray2.80A2-309[»]
ProteinModelPortaliP76077
SMRiP76077
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP76077

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni103 – 106Substrate binding1 Publication4
Regioni202 – 204Substrate binding1 Publication3

Phylogenomic databases

eggNOGiENOG4105CRS Bacteria
COG3396 LUCA
HOGENOMiHOG000249745
InParanoidiP76077
KOiK02609
OMAiDRWWWPS
PhylomeDBiP76077

Family and domain databases

Gene3Di1.20.1260.10, 1 hit
InterProiView protein in InterPro
IPR012347 Ferritin-like
IPR009078 Ferritin-like_SF
IPR011881 PaaA
IPR007814 PaaA_PaaC
PfamiView protein in Pfam
PF05138 PaaA_PaaC, 1 hit
SUPFAMiSSF47240 SSF47240, 1 hit
TIGRFAMsiTIGR02156 PA_CoA_Oxy1, 1 hit

Sequencei

Sequence statusi: Complete.

P76077-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTQEERFEQR IAQETAIEPQ DWMPDAYRKT LIRQIGQHAH SEIVGMLPEG
60 70 80 90 100
NWITRAPTLR RKAILLAKVQ DEAGHGLYLY SAAETLGCAR EDIYQKMLDG
110 120 130 140 150
RMKYSSIFNY PTLSWADIGV IGWLVDGAAI VNQVALCRTS YGPYARAMVK
160 170 180 190 200
ICKEESFHQR QGFEACMALA QGSEAQKQML QDAINRFWWP ALMMFGPNDD
210 220 230 240 250
NSPNSARSLT WKIKRFTNDE LRQRFVDNTV PQVEMLGMTV PDPDLHFDTE
260 270 280 290 300
SGHYRFGEID WQEFNEVING RGICNQERLD AKRKAWEEGT WVREAALAHA

QKQHARKVA
Length:309
Mass (Da):35,499
Last modified:February 1, 1997 - v1
Checksum:i83AEC6D7AC084073
GO

Sequence cautioni

The sequence CAA66090 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural varianti210T → A in strain: W. 1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X97452 Genomic DNA Translation: CAA66090.1 Different initiation.
U00096 Genomic DNA Translation: AAC74470.1
AP009048 Genomic DNA Translation: BAE76423.1
PIRiG64889
RefSeqiNP_415906.1, NC_000913.3
WP_000191077.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaiAAC74470; AAC74470; b1388
BAE76423; BAE76423; BAE76423
GeneIDi945833
KEGGiecj:JW1383
eco:b1388
PATRICifig|1411691.4.peg.883

Similar proteinsi

Entry informationi

Entry nameiPAAA_ECOLI
AccessioniPrimary (citable) accession number: P76077
Secondary accession number(s): O53010, Q2MBD3
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: February 1, 1997
Last modified: March 28, 2018
This is version 120 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome
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Main funding by: National Institutes of Health