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Protein

tRNA-cytidine(32) 2-sulfurtransferase

Gene

ttcA

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes the ATP-dependent 2-thiolation of cytidine in position 32 of tRNA, to form 2-thiocytidine (s2C32). The sulfur atoms are provided by the cysteine/cysteine desulfurase (IscS) system.UniRule annotation1 Publication1 Publication

Miscellaneous

The thiolation reaction likely consists of two steps: a first activation step by ATP to form an adenylated intermediate of the target base of tRNA, and a second nucleophilic substitution step of the sulfur (S) atom supplied by the hydrosulfide attached to the Fe-S cluster.1 Publication

Cofactori

Protein has several cofactor binding sites:

Pathwayi: tRNA modification

This protein is involved in tRNA modification.1 Publication
View all proteins of this organism that are known to be involved in tRNA modification.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi122Iron-sulfur (4Fe-4S)By similarity1 Publication1
Metal bindingi125Iron-sulfur (4Fe-4S)By similarity1 Publication1
Metal bindingi213Iron-sulfur (4Fe-4S)By similarity1 Publication1

GO - Molecular functioni

  • 4 iron, 4 sulfur cluster binding Source: EcoCyc
  • ATP binding Source: UniProtKB-KW
  • metal ion binding Source: UniProtKB-KW
  • sulfurtransferase activity Source: EcoCyc
  • tRNA binding Source: UniProtKB-KW

GO - Biological processi

  • tRNA modification Source: EcoCyc
  • tRNA thio-modification Source: EcoCyc

Keywordsi

Molecular functionRNA-binding, Transferase, tRNA-binding
Biological processtRNA processing
Ligand4Fe-4S, ATP-binding, Iron, Iron-sulfur, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciEcoCyc:G6675-MONOMER
MetaCyc:G6675-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA-cytidine(32) 2-sulfurtransferaseUniRule annotation (EC:2.8.1.-1 Publication)
Alternative name(s):
Two-thiocytidine biosynthesis protein A1 Publication
tRNA 2-thiocytidine biosynthesis protein TtcAUniRule annotation
Gene namesi
Name:ttcA1 PublicationUniRule annotation
Synonyms:ydaO
Ordered Locus Names:b1344, JW1338
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG13357 ttcA

Subcellular locationi

  • Cytoplasm UniRule annotationCurated

GO - Cellular componenti

  • cytosol Source: EcoCyc

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Disruption phenotypei

Loss of cytidine thiolation in position 32 in tRNA.1 Publication

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi45C → A: Able to functionally complement a ttcA deletion mutant as efficiently as wild-type. 1 Publication1
Mutagenesisi122C → A: Not able to functionally complement a ttcA deletion mutant. 1 Publication1
Mutagenesisi125C → A: Not able to functionally complement a ttcA deletion mutant. 1 Publication1
Mutagenesisi191C → A: Able to functionally complement a ttcA deletion mutant as efficiently as wild-type. 1 Publication1
Mutagenesisi210C → A: Able to functionally complement a ttcA deletion mutant, although less efficiently than wild-type. 1 Publication1
Mutagenesisi213C → A: Not able to functionally complement a ttcA deletion mutant. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001689071 – 311tRNA-cytidine(32) 2-sulfurtransferaseAdd BLAST311

Proteomic databases

PaxDbiP76055
PRIDEiP76055

Interactioni

Subunit structurei

Homodimer.1 Publication

Protein-protein interaction databases

BioGridi4261949, 118 interactors
IntActiP76055, 2 interactors
STRINGi316385.ECDH10B_1465

Structurei

3D structure databases

ProteinModelPortaliP76055
SMRiP76055
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi47 – 52PP-loop motif1 Publication6

Sequence similaritiesi

Belongs to the TtcA family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105CB3 Bacteria
COG0037 LUCA
HOGENOMiHOG000013323
InParanoidiP76055
KOiK14058
OMAiKPLKYSY
PhylomeDBiP76055

Family and domain databases

Gene3Di3.40.50.620, 1 hit
HAMAPiMF_01850 TtcA, 1 hit
InterProiView protein in InterPro
IPR014729 Rossmann-like_a/b/a_fold
IPR011063 TilS/TtcA_N
IPR012089 tRNA_Cyd_32_2_STrfase
IPR035107 tRNA_thiolation_TtcA_Ctu1
PfamiView protein in Pfam
PF01171 ATP_bind_3, 1 hit
PIRSFiPIRSF004976 ATPase_YdaO, 1 hit

Sequencei

Sequence statusi: Complete.

P76055-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MQENQQITKK EQYNLNKLQK RLRRNVGEAI ADFNMIEEGD RIMVCLSGGK
60 70 80 90 100
DSYTMLEILR NLQQSAPINF SLVAVNLDQK QPGFPEHVLP EYLEKLGVEY
110 120 130 140 150
KIVEENTYGI VKEKIPEGKT TCSLCSRLRR GILYRTATEL GATKIALGHH
160 170 180 190 200
RDDILQTLFL NMFYGGKMKG MPPKLMSDDG KHIVIRPLAY CREKDIQRFA
210 220 230 240 250
DAKAFPIIPC NLCGSQPNLQ RQVIADMLRD WDKRYPGRIE TMFSAMQNVV
260 270 280 290 300
PSHLCDTNLF DFKGITHGSE VVNGGDLAFD REEIPLQPAC WQPEEDENQL
310
DELRLNVVEV K
Length:311
Mass (Da):35,561
Last modified:February 1, 1997 - v1
Checksum:iD696057B7C092D79
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti11E → A in BAA04675 (PubMed:7764507).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00096 Genomic DNA Translation: AAC74426.1
AP009048 Genomic DNA Translation: BAE76407.1
D21139 Genomic DNA Translation: BAA04675.1
PIRiC64884
RefSeqiNP_415860.1, NC_000913.3
WP_001157406.1, NZ_CP014272.1

Genome annotation databases

EnsemblBacteriaiAAC74426; AAC74426; b1344
BAE76407; BAE76407; BAE76407
GeneIDi948967
KEGGiecj:JW1338
eco:b1344
PATRICifig|1411691.4.peg.932

Similar proteinsi

Entry informationi

Entry nameiTTCA_ECOLI
AccessioniPrimary (citable) accession number: P76055
Secondary accession number(s): Q2MBE9, Q47558
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: February 1, 1997
Last modified: March 28, 2018
This is version 124 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health