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Protein

tRNA 2-thiocytidine biosynthesis protein TtcA

Gene

ttcA

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Required for the thiolation of cytidine in position 32 of tRNA, to form 2-thiocytidine (s2C32).UniRule annotation1 Publication

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi47 – 52ATPUniRule annotation6

GO - Molecular functioni

  • 4 iron, 4 sulfur cluster binding Source: EcoCyc
  • ATP binding Source: UniProtKB-HAMAP
  • sulfurtransferase activity Source: EcoCyc

GO - Biological processi

  • tRNA modification Source: EcoCyc
  • tRNA thio-modification Source: EcoCyc
Complete GO annotation...

Keywords - Biological processi

tRNA processing

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciEcoCyc:G6675-MONOMER.
ECOL316407:JW1338-MONOMER.
MetaCyc:G6675-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA 2-thiocytidine biosynthesis protein TtcAUniRule annotation
Gene namesi
Name:ttcAUniRule annotation
Synonyms:ydaO
Ordered Locus Names:b1344, JW1338
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG13357. ttcA.

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

  • cytosol Source: EcoCyc
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Disruption phenotypei

Loss of cytidine thiolation in position 32 of some tRNA species.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001689071 – 311tRNA 2-thiocytidine biosynthesis protein TtcAAdd BLAST311

Proteomic databases

PaxDbiP76055.
PRIDEiP76055.

Interactioni

Protein-protein interaction databases

BioGridi4261949. 37 interactors.
IntActiP76055. 2 interactors.
STRINGi511145.b1344.

Structurei

3D structure databases

ProteinModelPortaliP76055.
SMRiP76055.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi122 – 125CXXC4
Motifi210 – 213CXXC4

Sequence similaritiesi

Belongs to the TtcA family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105CB3. Bacteria.
COG0037. LUCA.
HOGENOMiHOG000013323.
InParanoidiP76055.
KOiK14058.
OMAiNMIEEND.
PhylomeDBiP76055.

Family and domain databases

Gene3Di3.40.50.620. 1 hit.
HAMAPiMF_01850. TtcA. 1 hit.
InterProiIPR012089. 2-thiocytidine_tRNA_synth_TtcA.
IPR014729. Rossmann-like_a/b/a_fold.
IPR011063. TilS/TtcA_N.
[Graphical view]
PfamiPF01171. ATP_bind_3. 1 hit.
[Graphical view]
PIRSFiPIRSF004976. ATPase_YdaO. 1 hit.

Sequencei

Sequence statusi: Complete.

P76055-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MQENQQITKK EQYNLNKLQK RLRRNVGEAI ADFNMIEEGD RIMVCLSGGK
60 70 80 90 100
DSYTMLEILR NLQQSAPINF SLVAVNLDQK QPGFPEHVLP EYLEKLGVEY
110 120 130 140 150
KIVEENTYGI VKEKIPEGKT TCSLCSRLRR GILYRTATEL GATKIALGHH
160 170 180 190 200
RDDILQTLFL NMFYGGKMKG MPPKLMSDDG KHIVIRPLAY CREKDIQRFA
210 220 230 240 250
DAKAFPIIPC NLCGSQPNLQ RQVIADMLRD WDKRYPGRIE TMFSAMQNVV
260 270 280 290 300
PSHLCDTNLF DFKGITHGSE VVNGGDLAFD REEIPLQPAC WQPEEDENQL
310
DELRLNVVEV K
Length:311
Mass (Da):35,561
Last modified:February 1, 1997 - v1
Checksum:iD696057B7C092D79
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti11E → A in BAA04675 (PubMed:7764507).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00096 Genomic DNA. Translation: AAC74426.1.
AP009048 Genomic DNA. Translation: BAE76407.1.
D21139 Genomic DNA. Translation: BAA04675.1.
PIRiC64884.
RefSeqiNP_415860.1. NC_000913.3.
WP_001157406.1. NZ_CP014272.1.

Genome annotation databases

EnsemblBacteriaiAAC74426; AAC74426; b1344.
BAE76407; BAE76407; BAE76407.
GeneIDi948967.
KEGGiecj:JW1338.
eco:b1344.
PATRICi32117968. VBIEscCol129921_1404.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00096 Genomic DNA. Translation: AAC74426.1.
AP009048 Genomic DNA. Translation: BAE76407.1.
D21139 Genomic DNA. Translation: BAA04675.1.
PIRiC64884.
RefSeqiNP_415860.1. NC_000913.3.
WP_001157406.1. NZ_CP014272.1.

3D structure databases

ProteinModelPortaliP76055.
SMRiP76055.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4261949. 37 interactors.
IntActiP76055. 2 interactors.
STRINGi511145.b1344.

Proteomic databases

PaxDbiP76055.
PRIDEiP76055.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC74426; AAC74426; b1344.
BAE76407; BAE76407; BAE76407.
GeneIDi948967.
KEGGiecj:JW1338.
eco:b1344.
PATRICi32117968. VBIEscCol129921_1404.

Organism-specific databases

EchoBASEiEB3140.
EcoGeneiEG13357. ttcA.

Phylogenomic databases

eggNOGiENOG4105CB3. Bacteria.
COG0037. LUCA.
HOGENOMiHOG000013323.
InParanoidiP76055.
KOiK14058.
OMAiNMIEEND.
PhylomeDBiP76055.

Enzyme and pathway databases

BioCyciEcoCyc:G6675-MONOMER.
ECOL316407:JW1338-MONOMER.
MetaCyc:G6675-MONOMER.

Miscellaneous databases

PROiP76055.

Family and domain databases

Gene3Di3.40.50.620. 1 hit.
HAMAPiMF_01850. TtcA. 1 hit.
InterProiIPR012089. 2-thiocytidine_tRNA_synth_TtcA.
IPR014729. Rossmann-like_a/b/a_fold.
IPR011063. TilS/TtcA_N.
[Graphical view]
PfamiPF01171. ATP_bind_3. 1 hit.
[Graphical view]
PIRSFiPIRSF004976. ATPase_YdaO. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiTTCA_ECOLI
AccessioniPrimary (citable) accession number: P76055
Secondary accession number(s): Q2MBE9, Q47558
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: February 1, 1997
Last modified: November 2, 2016
This is version 115 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.