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Protein

Probable DNA endonuclease SmrA

Gene

smrA

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Experimental evidence at protein leveli

Functioni

Has DNA endonuclease activity. Binds DNA.1 Publication

GO - Molecular functioni

  • DNA binding Source: EcoCyc
  • endodeoxyribonuclease activity Source: EcoCyc
Complete GO annotation...

Keywords - Molecular functioni

Endonuclease, Hydrolase, Nuclease

Keywords - Ligandi

DNA-binding

Enzyme and pathway databases

BioCyciEcoCyc:G6672-MONOMER.
ECOL316407:JW1334-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Probable DNA endonuclease SmrA (EC:3.1.-.-)
Gene namesi
Name:smrA
Synonyms:ydaL
Ordered Locus Names:b1340, JW1334
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG13354. smrA.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001689061 – 187Probable DNA endonuclease SmrAAdd BLAST187

Proteomic databases

PaxDbiP76053.
PRIDEiP76053.

Interactioni

Protein-protein interaction databases

BioGridi4262880. 6 interactors.
IntActiP76053. 1 interactor.
STRINGi511145.b1340.

Structurei

Secondary structure

1187
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi43 – 45Combined sources3
Beta strandi63 – 65Combined sources3
Turni71 – 74Combined sources4
Helixi75 – 78Combined sources4
Helixi84 – 86Combined sources3
Beta strandi87 – 89Combined sources3
Helixi95 – 111Combined sources17
Beta strandi115 – 120Combined sources6
Helixi130 – 143Combined sources14
Beta strandi148 – 153Combined sources6
Helixi156 – 158Combined sources3
Helixi160 – 162Combined sources3
Beta strandi163 – 168Combined sources6

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3QD7X-ray2.30X39-175[»]
ProteinModelPortaliP76053.
SMRiP76053.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP76053.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini88 – 169SmrPROSITE-ProRule annotationAdd BLAST82

Sequence similaritiesi

Contains 1 Smr domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiENOG4108DG9. Bacteria.
COG2840. LUCA.
HOGENOMiHOG000257489.
InParanoidiP76053.
OMAiDDSHANI.
PhylomeDBiP76053.

Family and domain databases

InterProiIPR002625. Smr_dom.
[Graphical view]
PfamiPF01713. Smr. 1 hit.
[Graphical view]
SMARTiSM00463. SMR. 1 hit.
[Graphical view]
SUPFAMiSSF160443. SSF160443. 1 hit.
PROSITEiPS50828. SMR. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P76053-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNLDDKSLFL DAMEDVQPLK RATDVHWHPT RNQRAPQRID TLQLDNFLTT
60 70 80 90 100
GFLDIIPLSQ PLEFRREGLQ HGVLDKLRSG KYPQQASLNL LRQPVEECRK
110 120 130 140 150
MVFSFIQQAL ADGLRNVLII HGKGRDDKSH ANIVRSYVAR WLTEFDDVQA
160 170 180
YCTALPHHGG SGACYVALRK TAQAKQENWE RHAKRSR
Length:187
Mass (Da):21,512
Last modified:February 1, 1997 - v1
Checksum:i5FC4F67116D92129
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti40D → E AA sequence (PubMed:21276852).Curated1
Sequence conflicti45D → E AA sequence (PubMed:21276852).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00096 Genomic DNA. Translation: AAC74422.1.
AP009048 Genomic DNA. Translation: BAE76405.1.
PIRiG64883.
RefSeqiNP_415856.1. NC_000913.3.
WP_001046829.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC74422; AAC74422; b1340.
BAE76405; BAE76405; BAE76405.
GeneIDi945953.
KEGGiecj:JW1334.
eco:b1340.
PATRICi32117958. VBIEscCol129921_1399.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00096 Genomic DNA. Translation: AAC74422.1.
AP009048 Genomic DNA. Translation: BAE76405.1.
PIRiG64883.
RefSeqiNP_415856.1. NC_000913.3.
WP_001046829.1. NZ_LN832404.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3QD7X-ray2.30X39-175[»]
ProteinModelPortaliP76053.
SMRiP76053.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4262880. 6 interactors.
IntActiP76053. 1 interactor.
STRINGi511145.b1340.

Proteomic databases

PaxDbiP76053.
PRIDEiP76053.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC74422; AAC74422; b1340.
BAE76405; BAE76405; BAE76405.
GeneIDi945953.
KEGGiecj:JW1334.
eco:b1340.
PATRICi32117958. VBIEscCol129921_1399.

Organism-specific databases

EchoBASEiEB3137.
EcoGeneiEG13354. smrA.

Phylogenomic databases

eggNOGiENOG4108DG9. Bacteria.
COG2840. LUCA.
HOGENOMiHOG000257489.
InParanoidiP76053.
OMAiDDSHANI.
PhylomeDBiP76053.

Enzyme and pathway databases

BioCyciEcoCyc:G6672-MONOMER.
ECOL316407:JW1334-MONOMER.

Miscellaneous databases

EvolutionaryTraceiP76053.
PROiP76053.

Family and domain databases

InterProiIPR002625. Smr_dom.
[Graphical view]
PfamiPF01713. Smr. 1 hit.
[Graphical view]
SMARTiSM00463. SMR. 1 hit.
[Graphical view]
SUPFAMiSSF160443. SSF160443. 1 hit.
PROSITEiPS50828. SMR. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiSMRA_ECOLI
AccessioniPrimary (citable) accession number: P76053
Secondary accession number(s): Q2MBF1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: February 1, 1997
Last modified: November 2, 2016
This is version 98 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.