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Protein

p-aminobenzoyl-glutamate hydrolase subunit B

Gene

abgB

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Component of the p-aminobenzoyl-glutamate hydrolase multicomponent enzyme system which catalyzes the cleavage of p-aminobenzoyl-glutamate (PABA-GLU) to form p-aminobenzoate (PABA) and glutamate. AbgAB does not degrade dipeptides and the physiological role of abgABT should be clarified.2 Publications

Cofactori

Mn2+1 Publication

Kineticsi

  1. KM=60 µM for PABA-GLU (at pH 8.5)1 Publication

    GO - Molecular functioni

    • para-aminobenzoyl-glutamate hydrolase activity Source: UniProtKB
    • protein heterodimerization activity Source: EcoliWiki

    GO - Biological processi

    • folic acid catabolic process Source: EcoliWiki

    Keywordsi

    Molecular functionHydrolase

    Enzyme and pathway databases

    BioCyciEcoCyc:G6669-MONOMER.
    MetaCyc:G6669-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    p-aminobenzoyl-glutamate hydrolase subunit B (EC:3.5.1.-)
    Alternative name(s):
    PABA-GLU hydrolase
    Short name:
    PGH
    Gene namesi
    Name:abgB
    Synonyms:ydaI
    Ordered Locus Names:b1337, JW1331
    ORF Names:ECK1333
    OrganismiEscherichia coli (strain K12)
    Taxonomic identifieri83333 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
    Proteomesi
    • UP000000318 Componenti: Chromosome
    • UP000000625 Componenti: Chromosome

    Organism-specific databases

    EcoGeneiEG13351. abgB.

    Subcellular locationi

    GO - Cellular componenti

    • cytoplasm Source: EcoliWiki

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    ChainiPRO_00000644211 – 481p-aminobenzoyl-glutamate hydrolase subunit BAdd BLAST481

    Proteomic databases

    PaxDbiP76052.
    PRIDEiP76052.

    Expressioni

    Inductioni

    Could be transcriptionally regulated by AbgR.1 Publication

    Interactioni

    Subunit structurei

    Forms a heterodimer with AbgA.1 Publication

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    abgAP773572EBI-1123629,EBI-9155452

    GO - Molecular functioni

    • protein heterodimerization activity Source: EcoliWiki

    Protein-protein interaction databases

    BioGridi4260157. 63 interactors.
    IntActiP76052. 6 interactors.
    STRINGi316385.ECDH10B_1457.

    Structurei

    3D structure databases

    ProteinModelPortaliP76052.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Phylogenomic databases

    eggNOGiENOG4107RT5. Bacteria.
    COG1473. LUCA.
    HOGENOMiHOG000251262.
    InParanoidiP76052.
    KOiK12941.
    PhylomeDBiP76052.

    Family and domain databases

    CDDicd05673. M20_Acy1L2_AbgB. 1 hit.
    InterProiView protein in InterPro
    IPR017439. Amidohydrolase.
    IPR017145. Aminobenzoyl-glu_utiliz_pB.
    IPR002933. Peptidase_M20.
    IPR011650. Peptidase_M20_dimer.
    PfamiView protein in Pfam
    PF01546. Peptidase_M20. 1 hit.
    PIRSFiPIRSF037227. Aminobenzoyl-glu_utiliz_pB. 1 hit.
    SUPFAMiSSF55031. SSF55031. 1 hit.
    TIGRFAMsiTIGR01891. amidohydrolases. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    P76052-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MQEIYRFIDD AIEADRQRYT DIADQIWDHP ETRFEEFWSA EHLASALESA
    60 70 80 90 100
    GFTVTRNVGN IPNAFIASFG QGKPVIALLG EYDALAGLSQ QAGCAQPTSV
    110 120 130 140 150
    TPGENGHGCG HNLLGTAAFA AAIAVKKWLE QYGQGGTVRF YGCPGEEGGS
    160 170 180 190 200
    GKTFMVREGV FDDVDAALTW HPEAFAGMFN TRTLANIQAS WRFKGIAAHA
    210 220 230 240 250
    ANSPHLGRSA LDAVTLMTTG TNFLNEHIIE KARVHYAITN SGGISPNVVQ
    260 270 280 290 300
    AQAEVLYLIR APEMTDVQHI YDRVAKIAEG AALMTETTVE CRFDKACSSY
    310 320 330 340 350
    LPNRTLENAM YQALSHFGTP EWNSEELAFA KQIQATLTSN DRQNSLNNIA
    360 370 380 390 400
    ATGGENGKVF ALRHRETVLA NEVAPYAATD NVLAASTDVG DVSWKLPVAQ
    410 420 430 440 450
    CFSPCFAVGT PLHTWQLVSQ GRTSIAHKGM LLAAKTMAAT TVNLFLDSGL
    460 470 480
    LQECQQEHQQ VTDTQPYHCP IPKNVTPSPL K
    Length:481
    Mass (Da):52,194
    Last modified:February 1, 1997 - v1
    Checksum:i84B09100A563E07C
    GO

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    U00096 Genomic DNA. Translation: AAC74419.1.
    AP009048 Genomic DNA. Translation: BAE76404.1.
    PIRiD64883.
    RefSeqiNP_415853.1. NC_000913.3.
    WP_001156451.1. NZ_LN832404.1.

    Genome annotation databases

    EnsemblBacteriaiAAC74419; AAC74419; b1337.
    BAE76404; BAE76404; BAE76404.
    GeneIDi945950.
    KEGGiecj:JW1331.
    eco:b1337.
    PATRICifig|1411691.4.peg.940.

    Similar proteinsi

    Entry informationi

    Entry nameiABGB_ECOLI
    AccessioniPrimary (citable) accession number: P76052
    Secondary accession number(s): Q2MBF2
    Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 11, 2001
    Last sequence update: February 1, 1997
    Last modified: August 30, 2017
    This is version 122 of the entry and version 1 of the sequence. See complete history.
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. Escherichia coli
      Escherichia coli (strain K12): entries and cross-references to EcoGene