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Protein

Glucosylglycerate phosphorylase

Gene

ycjM

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes the reversible phosphorolysis of glucosylglycerate into alpha-D-glucose 1-phosphate (Glc1P) and D-glycerate. May be a regulator of intracellular levels of glucosylglycerate, a compatible solute that primarily protects organisms facing salt stress and very specific nutritional constraints. Cannot catalyze the phosphorolysis of sucrose.1 Publication

Catalytic activityi

2-O-(alpha-D-glucopyranosyl)-D-glycerate + phosphate = alpha-D-glucopyranose 1-phosphate + D-glycerate.1 Publication

Kineticsi

  1. KM=3.4 mM for D-glycerate (at pH 6.5 and 30 degrees Celsius)1 Publication

    Sites

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Active sitei229NucleophileBy similarity1

    GO - Molecular functioni

    GO - Biological processi

    Keywordsi

    Molecular functionGlycosyltransferase, Transferase
    Biological processCarbohydrate metabolism

    Enzyme and pathway databases

    BioCyciEcoCyc:G6647-MONOMER

    Protein family/group databases

    CAZyiGH13 Glycoside Hydrolase Family 13

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Glucosylglycerate phosphorylase1 Publication (EC:2.4.1.3521 Publication)
    Short name:
    GGa phosphorylase1 Publication
    Short name:
    GGaP1 Publication
    Gene namesi
    Name:ycjM
    Ordered Locus Names:b1309, JW1302
    OrganismiEscherichia coli (strain K12)
    Taxonomic identifieri83333 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
    Proteomesi
    • UP000000318 Componenti: Chromosome
    • UP000000625 Componenti: Chromosome

    Organism-specific databases

    EcoGeneiEG13910 ycjM

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    ChainiPRO_00000722991 – 559Glucosylglycerate phosphorylaseAdd BLAST559

    Proteomic databases

    PaxDbiP76041
    PRIDEiP76041

    Interactioni

    Protein-protein interaction databases

    BioGridi4263230, 11 interactors
    IntActiP76041, 2 interactors
    STRINGi316385.ECDH10B_1426

    Structurei

    3D structure databases

    ProteinModelPortaliP76041
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Phylogenomic databases

    eggNOGiENOG4107QW3 Bacteria
    COG0366 LUCA
    HOGENOMiHOG000089743
    InParanoidiP76041
    KOiK00690
    OMAiNFTASHD
    PhylomeDBiP76041

    Family and domain databases

    CDDicd11356 AmyAc_Sucrose_phosphorylase-li, 1 hit
    InterProiView protein in InterPro
    IPR033746 GGa_phosphorylase
    IPR006047 Glyco_hydro_13_cat_dom
    IPR017853 Glycoside_hydrolase_SF
    IPR016377 Sucrose_GGa_phosphorylase-rel
    PfamiView protein in Pfam
    PF00128 Alpha-amylase, 1 hit
    PIRSFiPIRSF003059 Sucrose_phosphorylase, 1 hit
    SMARTiView protein in SMART
    SM00642 Aamy, 1 hit
    SUPFAMiSSF51445 SSF51445, 2 hits

    Sequencei

    Sequence statusi: Complete.

    P76041-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MKQKITDYLD EIYGGTFTAT HLQKLVTRLE SAKRLITQRR KKHWDESDVV
    60 70 80 90 100
    LITYADQFHS NDLKPLPTFN QFYHQWLQSI FSHVHLLPFY PWSSDDGFSV
    110 120 130 140 150
    IDYHQVASEA GEWQDIQQLG ECSHLMFDFV CNHMSAKSEW FKNYLQQHPG
    160 170 180 190 200
    FEDFFIAVDP QTDLSAVTRP RALPLLTPFQ MRDHSTRHLW TTFSDDQIDL
    210 220 230 240 250
    NYRSPEVLLA MVDVLLCYLA KGAEYVRLDA VGFMWKEPGT SCIHLEKTHL
    260 270 280 290 300
    IIKLLRSIID NVAPGTVIIT ETNVPHKDNI AYFGAGDDEA HMVYQFSLPP
    310 320 330 340 350
    LVLHAVQKQN VEALCAWAQN LTLPSSNTTW FNFLASHDGI GLNPLRGLLP
    360 370 380 390 400
    ESEILELVEA LQQEGALVNW KNNPDGTRSP YEINVTYMDA LSRRESSDEE
    410 420 430 440 450
    RCARFILAHA ILLSFPGVPA IYIQSILGSR NDYAGVEKLG YNRAINRKKY
    460 470 480 490 500
    HSKEITRELN DEATLRHAVY HELSRLITLR RSHNEFHPDN NFTIDTINSS
    510 520 530 540 550
    VMRIPRSNAD GNCLTGLFNV SKNIQHVNIT NLHGRDLISE VDILGNEITL

    RPWQVMWIK
    Length:559
    Mass (Da):64,186
    Last modified:July 15, 1998 - v2
    Checksum:iC05B71B0E0E369C2
    GO

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    U00096 Genomic DNA Translation: AAC74391.2
    AP009048 Genomic DNA Translation: BAA14886.1
    PIRiH64879
    RefSeqiNP_415825.4, NC_000913.3
    WP_000810499.1, NZ_LN832404.1

    Genome annotation databases

    EnsemblBacteriaiAAC74391; AAC74391; b1309
    BAA14886; BAA14886; BAA14886
    GeneIDi945659
    KEGGiecj:JW1302
    eco:b1309
    PATRICifig|1411691.4.peg.970

    Similar proteinsi

    Entry informationi

    Entry nameiGGAP_ECOLI
    AccessioniPrimary (citable) accession number: P76041
    Secondary accession number(s): P78149, P78151
    Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 15, 1998
    Last sequence update: July 15, 1998
    Last modified: April 25, 2018
    This is version 128 of the entry and version 2 of the sequence. See complete history.
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Escherichia coli
      Escherichia coli (strain K12): entries and cross-references to EcoGene
    2. Glycosyl hydrolases
      Classification of glycosyl hydrolase families and list of entries
    3. SIMILARITY comments
      Index of protein domains and families

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