Putrescine importer PuuP - Escherichia coli (strain K12)

Contribute

Send feedback

Read comments (?) or add your own

P76037 (PUUP_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 111. History...

Clusters with 100%, 90%, 50% identity |

Documents (3) |

Third-party data

text xml rdf/xml gff fasta

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Putrescine importer PuuP

Gene names

Name:	puuP
Synonyms:	ycjJ
Ordered Locus Names:	b1296, JW1289

Organism

Escherichia coli (strain K12) [Reference proteome] [HAMAP]

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia ›

Protein attributes

Sequence length	461 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Involved in the uptake of putrescine. Ref.4 Ref.5
Pathway	Amine and polyamine degradation; putrescine degradation.
Subcellular location	Cell inner membrane; Multi-pass membrane protein Potential.
Induction	Repressed by PuuR. Ref.5
Sequence similarities	Belongs to the amino acid-polyamine-organocation (APC) superfamily.
Biophysicochemical properties	Kinetic parameters: K_M=3.7 µM for putrescine Ref.5 V_max=19.9 nmol/min/mg enzyme

Ontologies

Keywords
Biological process	Amino-acid transport Transport
Cellular component	Cell inner membrane Cell membrane Membrane
Domain	Transmembrane Transmembrane helix
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	putrescine catabolic process Inferred from mutant phenotype Ref.5. Source: EcoCyc response to DNA damage stimulus Inferred from expression pattern PubMed 11967071. Source: EcoliWiki
Cellular_component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from direct assay Ref.4. Source: EcoCyc
Molecular_function	amino acid transmembrane transporter activity Inferred from electronic annotation. Source: InterPro putrescine transmembrane transporter activity Inferred from experiment Ref.5. Source: EcoCyc solute:hydrogen symporter activity Inferred from direct assay Ref.4. Source: EcoCyc
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 461

461

Putrescine importer PuuP

PRO_0000054211

Regions

Transmembrane

27 – 47

Helical; Potential

Transmembrane

54 – 74

Helical; Potential

Transmembrane

100 – 120

Helical; Potential

Transmembrane

125 – 145

Helical; Potential

Transmembrane

162 – 182

Helical; Potential

Transmembrane

204 – 224

Helical; Potential

Transmembrane

242 – 262

Helical; Potential

Transmembrane

275 – 295

Helical; Potential

Transmembrane

344 – 364

Helical; Potential

Transmembrane

365 – 385

Helical; Potential

Transmembrane

399 – 419

Helical; Potential

Transmembrane

422 – 442

Helical; Potential

Experimental info

Mutagenesis

110

Y → X: The uptake activity is reduced to one-eighth of that of wild-type. Ref.5

Sequences

Sequence

Length

Mass (Da)

Tools

P76037 [UniParc].

Last modified December 15, 1998. Version 2.
Checksum: B027F1FA01C1B5BB
Show »

FASTA

461

50,853

        10         20         30         40         50         60 
MAINSPLNIA AQPGKTRLRK SLKLWQVVMM GLAYLTPMTV FDTFGIVSGI SDGHVPASYL 

        70         80         90        100        110        120 
LALAGVLFTA ISYGKLVRQF PEAGSAYTYA QKSINPHVGF MVGWSSLLDY LFLPMINVLL 

       130        140        150        160        170        180 
AKIYLSALFP EVPPWVWVVT FVAILTAANL KSVNLVANFN TLFVLVQISI MVVFIFLVVQ 

       190        200        210        220        230        240 
GLHKGEGVGT VWSLQPFISE NAHLIPIITG ATIVCFSFLG FDAVTTLSEE TPDAARVIPK 

       250        260        270        280        290        300 
AIFLTAVYGG VIFIAASFFM QLFFPDISRF KDPDAALPEI ALYVGGKLFQ SIFLCTTFVN 

       310        320        330        340        350        360 
TLASGLASHA SVSRLLYVMG RDNVFPERVF GYVHPKWRTP ALNVIMVGIV ALSALFFDLV 

       370        380        390        400        410        420 
TATALINFGA LVAFTFVNLS VFNHFWRRKG MNKSWKDHFH YLLMPLVGAL TVGVLWVNLE 

       430        440        450        460 
STSLTLGLVW ASLGGAYLWY LIRRYRKVPL YDGDRTPVSE T

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"A 570-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 28.0-40.1 min region on the linkage map." Aiba H., Baba T., Fujita K., Hayashi K., Inada T., Isono K., Itoh T., Kasai H., Kashimoto K., Kimura S., Kitakawa M., Kitagawa M., Makino K., Miki T., Mizobuchi K., Mori H., Mori T., Motomura K. Horiuchi T. DNA Res. 3:363-377(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[2]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[3]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], SEQUENCE REVISION. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[4]	"A novel putrescine utilization pathway involves gamma-glutamylated intermediates of Escherichia coli K-12." Kurihara S., Oda S., Kato K., Kim H.G., Koyanagi T., Kumagai H., Suzuki H. J. Biol. Chem. 280:4602-4608(2005) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION AS A PUTRESCINE IMPORTER, NOMENCLATURE. Strain: K12.
[5]	"The putrescine Importer PuuP of Escherichia coli K-12." Kurihara S., Tsuboi Y., Oda S., Kim H.G., Kumagai H., Suzuki H. J. Bacteriol. 191:2776-2782(2009) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION IN PUTRESCINE DEGRADATION AND AS A PUTRESCINE IMPORTER, MUTAGENESIS OF TYR-110, INDUCTION, BIOPHYSICOCHEMICAL PROPERTIES. Strain: K12.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U00096 Genomic DNA. Translation: AAC74378.2. AP009048 Genomic DNA. Translation: BAA14856.2.
PIR	C64878.
RefSeq	NP_415812.4. NC_000913.2. YP_489564.1. NC_007779.1.
3D structure databases
ProteinModelPortal	P76037.
SMR	P76037. Positions 20-380.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-11601N.
IntAct	P76037. 2 interactions.
MINT	MINT-1252906.
STRING	511145.b1296.
Protein family/group databases
TCDB	2.A.3.1.13. amino acid-polyamine-organocation (APC) family.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAC74378; AAC74378; b1296. BAA14856; BAA14856; BAA14856.
GeneID	12931145. 946287.
KEGG	ecj:Y75_p1271. eco:b1296.
PATRIC	32117862. VBIEscCol129921_1351.
Organism-specific databases
EchoBASE	EB3666.
EcoGene	EG13907. puuP.
Phylogenomic databases
eggNOG	COG0531.
HOGENOM	HOG000164060.
KO	K14052.
OMA	WINLEIT.
ProtClustDB	CLSK2459753.
Enzyme and pathway databases
BioCyc	EcoCyc:B1296-MONOMER. ECOL316407:JW1289-MONOMER.
UniPathway	UPA00188.
Gene expression databases
Genevestigator	P76037.
Family and domain databases
InterPro	IPR004841. AA-permease_dom. IPR002293. AA/rel_permease1. [Graphical view]
PANTHER	PTHR11785. PTHR11785. 1 hit.
Pfam	PF00324. AA_permease. 1 hit. [Graphical view]
PIRSF	PIRSF006060. AA_transporter. 1 hit.
PROSITE	PS00218. AMINO_ACID_PERMEASE_1. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PUUP_ECOLI

Accession

Primary (citable) accession number: P76037
Secondary accession number(s): P77557

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1998
Last sequence update:	December 15, 1998
Last modified:	May 1, 2013
This is version 111 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program

Relevant documents

Escherichia coli

Escherichia coli (strain K12): entries and cross-references to EcoGene

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Experimental info

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Protein family/group databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Gene expression databases

Family and domain databases

Entry information

Relevant documents