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Protein

Putrescine importer PuuP

Gene

puuP

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Involved in the uptake of putrescine.3 Publications

Kineticsi

  1. KM=3.7 µM for putrescine1 Publication
  1. Vmax=19.9 nmol/min/mg enzyme1 Publication

Pathwayi: putrescine degradation

This protein is involved in the pathway putrescine degradation, which is part of Amine and polyamine degradation.
View all proteins of this organism that are known to be involved in the pathway putrescine degradation and in Amine and polyamine degradation.

GO - Molecular functioni

GO - Biological processi

  • amino acid transport Source: UniProtKB-KW
  • cellular response to DNA damage stimulus Source: EcoliWiki
  • putrescine catabolic process Source: EcoCyc
  • putrescine transport Source: EcoCyc

Keywordsi

Biological processAmino-acid transport, Transport

Enzyme and pathway databases

BioCyciEcoCyc:B1296-MONOMER
MetaCyc:B1296-MONOMER
UniPathwayiUPA00188

Protein family/group databases

TCDBi2.A.3.1.13 the amino acid-polyamine-organocation (apc) family

Names & Taxonomyi

Protein namesi
Recommended name:
Putrescine importer PuuP
Gene namesi
Name:puuP
Synonyms:ycjJ
Ordered Locus Names:b1296, JW1289
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG13907 puuP

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei27 – 47HelicalSequence analysisAdd BLAST21
Transmembranei54 – 74HelicalSequence analysisAdd BLAST21
Transmembranei100 – 120HelicalSequence analysisAdd BLAST21
Transmembranei125 – 145HelicalSequence analysisAdd BLAST21
Transmembranei162 – 182HelicalSequence analysisAdd BLAST21
Transmembranei204 – 224HelicalSequence analysisAdd BLAST21
Transmembranei242 – 262HelicalSequence analysisAdd BLAST21
Transmembranei275 – 295HelicalSequence analysisAdd BLAST21
Transmembranei344 – 364HelicalSequence analysisAdd BLAST21
Transmembranei365 – 385HelicalSequence analysisAdd BLAST21
Transmembranei399 – 419HelicalSequence analysisAdd BLAST21
Transmembranei422 – 442HelicalSequence analysisAdd BLAST21

GO - Cellular componenti

  • integral component of membrane Source: UniProtKB-KW
  • plasma membrane Source: EcoCyc

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

Pathology & Biotechi

Disruption phenotypei

No visible growth phenotype, no change in extracellular putrescine levels, if the putrescine export operon sapBCDF is also deleted growth is slower.1 Publication

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi110Y → X: The uptake activity is reduced to one-eighth of that of wild-type. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000542111 – 461Putrescine importer PuuPAdd BLAST461

Proteomic databases

PaxDbiP76037
PRIDEiP76037

Expressioni

Inductioni

Repressed by PuuR.1 Publication

Interactioni

Protein-protein interaction databases

BioGridi4260139, 3 interactors
DIPiDIP-11601N
IntActiP76037, 3 interactors
STRINGi316385.ECDH10B_1413

Structurei

3D structure databases

ProteinModelPortaliP76037
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG4105CUB Bacteria
COG0531 LUCA
HOGENOMiHOG000164060
InParanoidiP76037
KOiK14052
OMAiMGQPRIW
PhylomeDBiP76037

Family and domain databases

InterProiView protein in InterPro
IPR004841 AA-permease/SLC12A_dom
IPR002293 AA/rel_permease1
PfamiView protein in Pfam
PF00324 AA_permease, 1 hit
PIRSFiPIRSF006060 AA_transporter, 1 hit

Sequencei

Sequence statusi: Complete.

P76037-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAINSPLNIA AQPGKTRLRK SLKLWQVVMM GLAYLTPMTV FDTFGIVSGI
60 70 80 90 100
SDGHVPASYL LALAGVLFTA ISYGKLVRQF PEAGSAYTYA QKSINPHVGF
110 120 130 140 150
MVGWSSLLDY LFLPMINVLL AKIYLSALFP EVPPWVWVVT FVAILTAANL
160 170 180 190 200
KSVNLVANFN TLFVLVQISI MVVFIFLVVQ GLHKGEGVGT VWSLQPFISE
210 220 230 240 250
NAHLIPIITG ATIVCFSFLG FDAVTTLSEE TPDAARVIPK AIFLTAVYGG
260 270 280 290 300
VIFIAASFFM QLFFPDISRF KDPDAALPEI ALYVGGKLFQ SIFLCTTFVN
310 320 330 340 350
TLASGLASHA SVSRLLYVMG RDNVFPERVF GYVHPKWRTP ALNVIMVGIV
360 370 380 390 400
ALSALFFDLV TATALINFGA LVAFTFVNLS VFNHFWRRKG MNKSWKDHFH
410 420 430 440 450
YLLMPLVGAL TVGVLWVNLE STSLTLGLVW ASLGGAYLWY LIRRYRKVPL
460
YDGDRTPVSE T
Length:461
Mass (Da):50,853
Last modified:December 15, 1998 - v2
Checksum:iB027F1FA01C1B5BB
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00096 Genomic DNA Translation: AAC74378.2
AP009048 Genomic DNA Translation: BAA14856.2
PIRiC64878
RefSeqiNP_415812.4, NC_000913.3

Genome annotation databases

EnsemblBacteriaiAAC74378; AAC74378; b1296
BAA14856; BAA14856; BAA14856
GeneIDi946287
KEGGiecj:JW1289
eco:b1296
PATRICifig|511145.12.peg.1351

Similar proteinsi

Entry informationi

Entry nameiPUUP_ECOLI
AccessioniPrimary (citable) accession number: P76037
Secondary accession number(s): P77557
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: December 15, 1998
Last modified: March 28, 2018
This is version 143 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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