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Protein

PTS-dependent dihydroxyacetone kinase operon regulatory protein

Gene

dhaR

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Positively regulates the dhaKLM operon from a sigma-70 promoter. Represses its own expression.2 Publications

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi355 – 362ATPPROSITE-ProRule annotation8
Nucleotide bindingi415 – 424ATPPROSITE-ProRule annotation10

GO - Molecular functioni

GO - Biological processi

  • glycerol metabolic process Source: UniProtKB-KW
  • regulation of transcription, DNA-templated Source: EcoCyc
  • transcription, DNA-templated Source: EcoCyc

Keywordsi

Molecular functionActivator, DNA-binding, Repressor
Biological processGlycerol metabolism, Transcription, Transcription regulation
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciEcoCyc:G6628-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
PTS-dependent dihydroxyacetone kinase operon regulatory protein1 Publication
Gene namesi
Name:dhaR1 Publication
Synonyms:ycgU
Ordered Locus Names:b1201, JW5188
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG13902 dhaR

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000813201 – 639PTS-dependent dihydroxyacetone kinase operon regulatory proteinAdd BLAST639

Proteomic databases

PaxDbiP76016
PRIDEiP76016

Expressioni

Inductioni

Represses its own expression. DhaL and DhaK act antagonistically as coactivator and corepressor of the transcription activator by mutually exclusive binding to the sensing domain of DhaR. In the presence of dihydroxyacetone, DhaK that binds dihydroxyacetone has less affinity for DhaR and is displaced by DhaL-ADP, which stimulates DhaR activity. In the absence of dihydroxyacetone, DhaL-ADP is converted by the PTS to DhaL-ATP, which does not bind to DhaR.1 Publication

Interactioni

Subunit structurei

Homodimer. DhaR forms complexes with DhaK and DhaL-ADP.1 Publication

Binary interactionsi

Show more details

GO - Molecular functioni

Protein-protein interaction databases

BioGridi4261628, 15 interactors
DIPiDIP-9438N
IntActiP76016, 2 interactors
STRINGi316385.ECDH10B_1254

Structurei

Secondary structure

1639
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi14 – 26Combined sources13
Helixi40 – 49Combined sources10
Helixi51 – 66Combined sources16
Beta strandi70 – 72Combined sources3
Beta strandi74 – 79Combined sources6
Beta strandi82 – 89Combined sources8
Helixi91 – 99Combined sources9
Helixi110 – 113Combined sources4
Helixi117 – 124Combined sources8
Beta strandi128 – 131Combined sources4
Helixi132 – 134Combined sources3
Beta strandi135 – 137Combined sources3
Helixi138 – 140Combined sources3
Beta strandi143 – 151Combined sources9
Beta strandi153 – 155Combined sources3
Beta strandi157 – 166Combined sources10
Helixi167 – 169Combined sources3
Helixi174 – 211Combined sources38
Beta strandi212 – 219Combined sources8
Beta strandi223 – 228Combined sources6
Helixi230 – 235Combined sources6
Turni240 – 242Combined sources3
Helixi248 – 251Combined sources4
Helixi256 – 263Combined sources8
Beta strandi268 – 276Combined sources9
Beta strandi281 – 293Combined sources13
Beta strandi296 – 304Combined sources9

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4LRXX-ray3.25C/D1-318[»]
4LRYX-ray2.83C/D1-318[»]
4LRZX-ray2.32E/F/G/H1-318[»]
ProteinModelPortaliP76016
SMRiP76016
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini52 – 189GAF1 PublicationAdd BLAST138
Domaini203 – 265PAS1 PublicationAdd BLAST63
Domaini327 – 552Sigma-54 factor interactionPROSITE-ProRule annotationAdd BLAST226

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 318Sensor domain1 PublicationAdd BLAST318

Phylogenomic databases

eggNOGiENOG4108JU9 Bacteria
COG3284 LUCA
HOGENOMiHOG000058487
InParanoidiP76016
KOiK05880
OMAiHVEVTFE
PhylomeDBiP76016

Family and domain databases

CDDicd00130 PAS, 1 hit
Gene3Di3.30.450.40, 1 hit
InterProiView protein in InterPro
IPR003593 AAA+_ATPase
IPR029016 GAF-like_dom_sf
IPR009057 Homeobox-like_sf
IPR002197 HTH_Fis
IPR027417 P-loop_NTPase
IPR000014 PAS
IPR035965 PAS-like_dom_sf
IPR013767 PAS_fold
IPR002078 Sigma_54_int
IPR025662 Sigma_54_int_dom_ATP-bd_1
IPR025943 Sigma_54_int_dom_ATP-bd_2
PfamiView protein in Pfam
PF02954 HTH_8, 1 hit
PF00989 PAS, 1 hit
PF00158 Sigma54_activat, 1 hit
SMARTiView protein in SMART
SM00382 AAA, 1 hit
SM00091 PAS, 1 hit
SUPFAMiSSF46689 SSF46689, 1 hit
SSF52540 SSF52540, 1 hit
SSF55785 SSF55785, 1 hit
PROSITEiView protein in PROSITE
PS00675 SIGMA54_INTERACT_1, 1 hit
PS00676 SIGMA54_INTERACT_2, 1 hit
PS50045 SIGMA54_INTERACT_4, 1 hit

Sequencei

Sequence statusi: Complete.

P76016-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSGAFNNDGR GISPLIATSW ERCNKLMKRE TWNVPHQAQG VTFASIYRRK
60 70 80 90 100
KAMLTLGQAA LEDAWEYMAP RECALFILDE TACILSRNGD PQTLQQLSAL
110 120 130 140 150
GFNDGTYCAE GIIGTCALSL AAISGQAVKT MADQHFKQVL WNWAFCATPL
160 170 180 190 200
FDSKGRLTGT IALACPVEQT TAADLPLTLA IAREVGNLLL TDSLLAETNR
210 220 230 240 250
HLNQLNALLE SMDDGVISWD EQGNLQFINA QAARVLRLDA TASQGRAITE
260 270 280 290 300
LLTLPAVLQQ AIKQAHPLKH VEATFESQHQ FIDAVITLKP IIETQGTSFI
310 320 330 340 350
LLLHPVEQMR QLMTSQLGKV SHTFAHMPQD DPQTRRLIHF GRQAARSSFP
360 370 380 390 400
VLLCGEEGVG KALLSQAIHN ESERAAGPYI AVNCELYGDA ALAEEFIGGD
410 420 430 440 450
RTDNENGRLS RLELAHGGTL FLEKIEYLAV ELQSALLQVI KQGVITRLDA
460 470 480 490 500
RRLIPIDVKV IATTTADLAM LVEQNRFSRQ LYYALHAFEI TIPPLRMRRG
510 520 530 540 550
SIPALVNNKL RSLEKRFSTR LKIDDDALAR LVSCAWPGND FELYSVIENL
560 570 580 590 600
ALSSDNGRIR VSDLPEHLFT EQATDDVSAT RLSTSLSFAE VEKEAIINAA
610 620 630
QVTGGRIQEM SALLGIGRTT LWRKMKQHGI DAGQFKRRV
Length:639
Mass (Da):70,562
Last modified:December 1, 2000 - v2
Checksum:i2DD327F3710E1CA7
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00096 Genomic DNA Translation: AAC74285.2
AP009048 Genomic DNA Translation: BAA36058.2
PIRiF64866
RefSeqiNP_415719.2, NC_000913.3
WP_001350505.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaiAAC74285; AAC74285; b1201
BAA36058; BAA36058; BAA36058
GeneIDi945743
KEGGiecj:JW5188
eco:b1201
PATRICifig|1411691.4.peg.1084

Similar proteinsi

Entry informationi

Entry nameiDHAR_ECOLI
AccessioniPrimary (citable) accession number: P76016
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: December 1, 2000
Last modified: March 28, 2018
This is version 144 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome
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Main funding by: National Institutes of Health