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Protein

PTS-dependent dihydroxyacetone kinase operon regulatory protein

Gene

dhaR

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Positively regulates the dhaKLM operon from a sigma-70 promoter. Represses its own synthesis.1 Publication

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi355 – 3628ATPPROSITE-ProRule annotation
Nucleotide bindingi415 – 42410ATPPROSITE-ProRule annotation
DNA bindingi608 – 62619H-T-H motifBy similarityAdd
BLAST

GO - Molecular functioni

GO - Biological processi

  • glycerol metabolic process Source: UniProtKB-KW
  • regulation of transcription, DNA-templated Source: EcoCyc
  • transcription, DNA-templated Source: EcoCyc
Complete GO annotation...

Keywords - Molecular functioni

Activator, Repressor

Keywords - Biological processi

Glycerol metabolism, Transcription, Transcription regulation

Keywords - Ligandi

ATP-binding, DNA-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciEcoCyc:G6628-MONOMER.
ECOL316407:JW5188-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
PTS-dependent dihydroxyacetone kinase operon regulatory protein
Gene namesi
Name:dhaR
Synonyms:ycgU
Ordered Locus Names:b1201, JW5188
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG13902. dhaR.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 639639PTS-dependent dihydroxyacetone kinase operon regulatory proteinPRO_0000081320Add
BLAST

Proteomic databases

PaxDbiP76016.
PRIDEiP76016.

Interactioni

Protein-protein interaction databases

BioGridi4261628. 15 interactions.
DIPiDIP-9438N.
IntActiP76016. 1 interaction.
STRINGi511145.b1201.

Structurei

Secondary structure

1
639
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi14 – 2613Combined sources
Helixi40 – 4910Combined sources
Helixi51 – 6616Combined sources
Beta strandi70 – 723Combined sources
Beta strandi74 – 796Combined sources
Beta strandi82 – 898Combined sources
Helixi91 – 999Combined sources
Helixi110 – 1134Combined sources
Helixi117 – 1248Combined sources
Beta strandi128 – 1314Combined sources
Helixi132 – 1343Combined sources
Beta strandi135 – 1373Combined sources
Helixi138 – 1403Combined sources
Beta strandi143 – 1519Combined sources
Beta strandi153 – 1553Combined sources
Beta strandi157 – 16610Combined sources
Helixi167 – 1693Combined sources
Helixi174 – 21138Combined sources
Beta strandi212 – 2198Combined sources
Beta strandi223 – 2286Combined sources
Helixi230 – 2356Combined sources
Turni240 – 2423Combined sources
Helixi248 – 2514Combined sources
Helixi256 – 2638Combined sources
Beta strandi268 – 2769Combined sources
Beta strandi281 – 29313Combined sources
Beta strandi296 – 3049Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4LRXX-ray3.25C/D1-318[»]
4LRYX-ray2.83C/D1-318[»]
4LRZX-ray2.32E/F/G/H1-318[»]
ProteinModelPortaliP76016.
SMRiP76016. Positions 13-306, 326-627.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini52 – 189138GAFAdd
BLAST
Domaini201 – 27171PASAdd
BLAST
Domaini319 – 543225Sigma-54 factor interactionPROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 318318Sensor domainAdd
BLAST

Sequence similaritiesi

Contains 1 GAF domain.Curated
Contains 1 PAS (PER-ARNT-SIM) domain.Curated
Contains 1 sigma-54 factor interaction domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiENOG4108JU9. Bacteria.
COG3284. LUCA.
HOGENOMiHOG000058487.
InParanoidiP76016.
KOiK05880.
OMAiDSTYDAI.
PhylomeDBiP76016.

Family and domain databases

Gene3Di1.10.10.60. 1 hit.
3.40.50.300. 1 hit.
InterProiIPR003593. AAA+_ATPase.
IPR009057. Homeodomain-like.
IPR002197. HTH_Fis.
IPR027417. P-loop_NTPase.
IPR000014. PAS.
IPR013767. PAS_fold.
IPR002078. Sigma_54_int.
IPR025662. Sigma_54_int_dom_ATP-bd_1.
IPR025943. Sigma_54_int_dom_ATP-bd_2.
[Graphical view]
PfamiPF02954. HTH_8. 1 hit.
PF00989. PAS. 1 hit.
PF00158. Sigma54_activat. 1 hit.
[Graphical view]
SMARTiSM00382. AAA. 1 hit.
SM00091. PAS. 1 hit.
[Graphical view]
SUPFAMiSSF46689. SSF46689. 1 hit.
SSF52540. SSF52540. 1 hit.
SSF55785. SSF55785. 1 hit.
PROSITEiPS00675. SIGMA54_INTERACT_1. 1 hit.
PS00676. SIGMA54_INTERACT_2. 1 hit.
PS50045. SIGMA54_INTERACT_4. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P76016-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSGAFNNDGR GISPLIATSW ERCNKLMKRE TWNVPHQAQG VTFASIYRRK
60 70 80 90 100
KAMLTLGQAA LEDAWEYMAP RECALFILDE TACILSRNGD PQTLQQLSAL
110 120 130 140 150
GFNDGTYCAE GIIGTCALSL AAISGQAVKT MADQHFKQVL WNWAFCATPL
160 170 180 190 200
FDSKGRLTGT IALACPVEQT TAADLPLTLA IAREVGNLLL TDSLLAETNR
210 220 230 240 250
HLNQLNALLE SMDDGVISWD EQGNLQFINA QAARVLRLDA TASQGRAITE
260 270 280 290 300
LLTLPAVLQQ AIKQAHPLKH VEATFESQHQ FIDAVITLKP IIETQGTSFI
310 320 330 340 350
LLLHPVEQMR QLMTSQLGKV SHTFAHMPQD DPQTRRLIHF GRQAARSSFP
360 370 380 390 400
VLLCGEEGVG KALLSQAIHN ESERAAGPYI AVNCELYGDA ALAEEFIGGD
410 420 430 440 450
RTDNENGRLS RLELAHGGTL FLEKIEYLAV ELQSALLQVI KQGVITRLDA
460 470 480 490 500
RRLIPIDVKV IATTTADLAM LVEQNRFSRQ LYYALHAFEI TIPPLRMRRG
510 520 530 540 550
SIPALVNNKL RSLEKRFSTR LKIDDDALAR LVSCAWPGND FELYSVIENL
560 570 580 590 600
ALSSDNGRIR VSDLPEHLFT EQATDDVSAT RLSTSLSFAE VEKEAIINAA
610 620 630
QVTGGRIQEM SALLGIGRTT LWRKMKQHGI DAGQFKRRV
Length:639
Mass (Da):70,562
Last modified:December 1, 2000 - v2
Checksum:i2DD327F3710E1CA7
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00096 Genomic DNA. Translation: AAC74285.2.
AP009048 Genomic DNA. Translation: BAA36058.2.
PIRiF64866.
RefSeqiNP_415719.2. NC_000913.3.
WP_001350505.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC74285; AAC74285; b1201.
BAA36058; BAA36058; BAA36058.
GeneIDi945743.
KEGGiecj:JW5188.
eco:b1201.
PATRICi32117652. VBIEscCol129921_1248.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00096 Genomic DNA. Translation: AAC74285.2.
AP009048 Genomic DNA. Translation: BAA36058.2.
PIRiF64866.
RefSeqiNP_415719.2. NC_000913.3.
WP_001350505.1. NZ_LN832404.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4LRXX-ray3.25C/D1-318[»]
4LRYX-ray2.83C/D1-318[»]
4LRZX-ray2.32E/F/G/H1-318[»]
ProteinModelPortaliP76016.
SMRiP76016. Positions 13-306, 326-627.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4261628. 15 interactions.
DIPiDIP-9438N.
IntActiP76016. 1 interaction.
STRINGi511145.b1201.

Proteomic databases

PaxDbiP76016.
PRIDEiP76016.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC74285; AAC74285; b1201.
BAA36058; BAA36058; BAA36058.
GeneIDi945743.
KEGGiecj:JW5188.
eco:b1201.
PATRICi32117652. VBIEscCol129921_1248.

Organism-specific databases

EchoBASEiEB3661.
EcoGeneiEG13902. dhaR.

Phylogenomic databases

eggNOGiENOG4108JU9. Bacteria.
COG3284. LUCA.
HOGENOMiHOG000058487.
InParanoidiP76016.
KOiK05880.
OMAiDSTYDAI.
PhylomeDBiP76016.

Enzyme and pathway databases

BioCyciEcoCyc:G6628-MONOMER.
ECOL316407:JW5188-MONOMER.

Miscellaneous databases

PROiP76016.

Family and domain databases

Gene3Di1.10.10.60. 1 hit.
3.40.50.300. 1 hit.
InterProiIPR003593. AAA+_ATPase.
IPR009057. Homeodomain-like.
IPR002197. HTH_Fis.
IPR027417. P-loop_NTPase.
IPR000014. PAS.
IPR013767. PAS_fold.
IPR002078. Sigma_54_int.
IPR025662. Sigma_54_int_dom_ATP-bd_1.
IPR025943. Sigma_54_int_dom_ATP-bd_2.
[Graphical view]
PfamiPF02954. HTH_8. 1 hit.
PF00989. PAS. 1 hit.
PF00158. Sigma54_activat. 1 hit.
[Graphical view]
SMARTiSM00382. AAA. 1 hit.
SM00091. PAS. 1 hit.
[Graphical view]
SUPFAMiSSF46689. SSF46689. 1 hit.
SSF52540. SSF52540. 1 hit.
SSF55785. SSF55785. 1 hit.
PROSITEiPS00675. SIGMA54_INTERACT_1. 1 hit.
PS00676. SIGMA54_INTERACT_2. 1 hit.
PS50045. SIGMA54_INTERACT_4. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiDHAR_ECOLI
AccessioniPrimary (citable) accession number: P76016
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: December 1, 2000
Last modified: September 7, 2016
This is version 133 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Miscellaneous

DhaK and DhaL-ADP bind to DhaR in a mutually exclusive manner, and act as corepressor and coactivator of DhaR, respectively. DhaK binds to the sensor domain of DhaR and keeps it in a transcription inactive state. In the presence of dihydroxyacetone, DhaR activity is activated by DhaL-ADP: DhaK that binds dihydroxyacetone has less affinity for DhaR and is displaced by DhaL-ADP, which activates DhaR.

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.