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Protein

PTS-dependent dihydroxyacetone kinase operon regulatory protein

Gene

dhaR

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Positively regulates the dhaKLM operon from a sigma-70 promoter. Represses its own synthesis.1 Publication

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi355 – 362ATPPROSITE-ProRule annotation8
Nucleotide bindingi415 – 424ATPPROSITE-ProRule annotation10
DNA bindingi608 – 626H-T-H motifBy similarityAdd BLAST19

GO - Molecular functioni

GO - Biological processi

  • glycerol metabolic process Source: UniProtKB-KW
  • regulation of transcription, DNA-templated Source: EcoCyc
  • transcription, DNA-templated Source: EcoCyc
Complete GO annotation...

Keywords - Molecular functioni

Activator, Repressor

Keywords - Biological processi

Glycerol metabolism, Transcription, Transcription regulation

Keywords - Ligandi

ATP-binding, DNA-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciEcoCyc:G6628-MONOMER.
ECOL316407:JW5188-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
PTS-dependent dihydroxyacetone kinase operon regulatory protein
Gene namesi
Name:dhaR
Synonyms:ycgU
Ordered Locus Names:b1201, JW5188
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG13902. dhaR.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000813201 – 639PTS-dependent dihydroxyacetone kinase operon regulatory proteinAdd BLAST639

Proteomic databases

PaxDbiP76016.
PRIDEiP76016.

Interactioni

Protein-protein interaction databases

BioGridi4261628. 15 interactors.
DIPiDIP-9438N.
IntActiP76016. 1 interactor.
STRINGi511145.b1201.

Structurei

Secondary structure

1639
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi14 – 26Combined sources13
Helixi40 – 49Combined sources10
Helixi51 – 66Combined sources16
Beta strandi70 – 72Combined sources3
Beta strandi74 – 79Combined sources6
Beta strandi82 – 89Combined sources8
Helixi91 – 99Combined sources9
Helixi110 – 113Combined sources4
Helixi117 – 124Combined sources8
Beta strandi128 – 131Combined sources4
Helixi132 – 134Combined sources3
Beta strandi135 – 137Combined sources3
Helixi138 – 140Combined sources3
Beta strandi143 – 151Combined sources9
Beta strandi153 – 155Combined sources3
Beta strandi157 – 166Combined sources10
Helixi167 – 169Combined sources3
Helixi174 – 211Combined sources38
Beta strandi212 – 219Combined sources8
Beta strandi223 – 228Combined sources6
Helixi230 – 235Combined sources6
Turni240 – 242Combined sources3
Helixi248 – 251Combined sources4
Helixi256 – 263Combined sources8
Beta strandi268 – 276Combined sources9
Beta strandi281 – 293Combined sources13
Beta strandi296 – 304Combined sources9

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4LRXX-ray3.25C/D1-318[»]
4LRYX-ray2.83C/D1-318[»]
4LRZX-ray2.32E/F/G/H1-318[»]
ProteinModelPortaliP76016.
SMRiP76016.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini52 – 189GAFAdd BLAST138
Domaini201 – 271PASAdd BLAST71
Domaini319 – 543Sigma-54 factor interactionPROSITE-ProRule annotationAdd BLAST225

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 318Sensor domainAdd BLAST318

Sequence similaritiesi

Contains 1 GAF domain.Curated
Contains 1 PAS (PER-ARNT-SIM) domain.Curated
Contains 1 sigma-54 factor interaction domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiENOG4108JU9. Bacteria.
COG3284. LUCA.
HOGENOMiHOG000058487.
InParanoidiP76016.
KOiK05880.
OMAiDSTYDAI.
PhylomeDBiP76016.

Family and domain databases

Gene3Di1.10.10.60. 1 hit.
3.40.50.300. 1 hit.
InterProiIPR003593. AAA+_ATPase.
IPR009057. Homeodomain-like.
IPR002197. HTH_Fis.
IPR027417. P-loop_NTPase.
IPR000014. PAS.
IPR013767. PAS_fold.
IPR002078. Sigma_54_int.
IPR025662. Sigma_54_int_dom_ATP-bd_1.
IPR025943. Sigma_54_int_dom_ATP-bd_2.
[Graphical view]
PfamiPF02954. HTH_8. 1 hit.
PF00989. PAS. 1 hit.
PF00158. Sigma54_activat. 1 hit.
[Graphical view]
SMARTiSM00382. AAA. 1 hit.
SM00091. PAS. 1 hit.
[Graphical view]
SUPFAMiSSF46689. SSF46689. 1 hit.
SSF52540. SSF52540. 1 hit.
SSF55785. SSF55785. 1 hit.
PROSITEiPS00675. SIGMA54_INTERACT_1. 1 hit.
PS00676. SIGMA54_INTERACT_2. 1 hit.
PS50045. SIGMA54_INTERACT_4. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P76016-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSGAFNNDGR GISPLIATSW ERCNKLMKRE TWNVPHQAQG VTFASIYRRK
60 70 80 90 100
KAMLTLGQAA LEDAWEYMAP RECALFILDE TACILSRNGD PQTLQQLSAL
110 120 130 140 150
GFNDGTYCAE GIIGTCALSL AAISGQAVKT MADQHFKQVL WNWAFCATPL
160 170 180 190 200
FDSKGRLTGT IALACPVEQT TAADLPLTLA IAREVGNLLL TDSLLAETNR
210 220 230 240 250
HLNQLNALLE SMDDGVISWD EQGNLQFINA QAARVLRLDA TASQGRAITE
260 270 280 290 300
LLTLPAVLQQ AIKQAHPLKH VEATFESQHQ FIDAVITLKP IIETQGTSFI
310 320 330 340 350
LLLHPVEQMR QLMTSQLGKV SHTFAHMPQD DPQTRRLIHF GRQAARSSFP
360 370 380 390 400
VLLCGEEGVG KALLSQAIHN ESERAAGPYI AVNCELYGDA ALAEEFIGGD
410 420 430 440 450
RTDNENGRLS RLELAHGGTL FLEKIEYLAV ELQSALLQVI KQGVITRLDA
460 470 480 490 500
RRLIPIDVKV IATTTADLAM LVEQNRFSRQ LYYALHAFEI TIPPLRMRRG
510 520 530 540 550
SIPALVNNKL RSLEKRFSTR LKIDDDALAR LVSCAWPGND FELYSVIENL
560 570 580 590 600
ALSSDNGRIR VSDLPEHLFT EQATDDVSAT RLSTSLSFAE VEKEAIINAA
610 620 630
QVTGGRIQEM SALLGIGRTT LWRKMKQHGI DAGQFKRRV
Length:639
Mass (Da):70,562
Last modified:December 1, 2000 - v2
Checksum:i2DD327F3710E1CA7
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00096 Genomic DNA. Translation: AAC74285.2.
AP009048 Genomic DNA. Translation: BAA36058.2.
PIRiF64866.
RefSeqiNP_415719.2. NC_000913.3.
WP_001350505.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC74285; AAC74285; b1201.
BAA36058; BAA36058; BAA36058.
GeneIDi945743.
KEGGiecj:JW5188.
eco:b1201.
PATRICi32117652. VBIEscCol129921_1248.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00096 Genomic DNA. Translation: AAC74285.2.
AP009048 Genomic DNA. Translation: BAA36058.2.
PIRiF64866.
RefSeqiNP_415719.2. NC_000913.3.
WP_001350505.1. NZ_LN832404.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4LRXX-ray3.25C/D1-318[»]
4LRYX-ray2.83C/D1-318[»]
4LRZX-ray2.32E/F/G/H1-318[»]
ProteinModelPortaliP76016.
SMRiP76016.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4261628. 15 interactors.
DIPiDIP-9438N.
IntActiP76016. 1 interactor.
STRINGi511145.b1201.

Proteomic databases

PaxDbiP76016.
PRIDEiP76016.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC74285; AAC74285; b1201.
BAA36058; BAA36058; BAA36058.
GeneIDi945743.
KEGGiecj:JW5188.
eco:b1201.
PATRICi32117652. VBIEscCol129921_1248.

Organism-specific databases

EchoBASEiEB3661.
EcoGeneiEG13902. dhaR.

Phylogenomic databases

eggNOGiENOG4108JU9. Bacteria.
COG3284. LUCA.
HOGENOMiHOG000058487.
InParanoidiP76016.
KOiK05880.
OMAiDSTYDAI.
PhylomeDBiP76016.

Enzyme and pathway databases

BioCyciEcoCyc:G6628-MONOMER.
ECOL316407:JW5188-MONOMER.

Miscellaneous databases

PROiP76016.

Family and domain databases

Gene3Di1.10.10.60. 1 hit.
3.40.50.300. 1 hit.
InterProiIPR003593. AAA+_ATPase.
IPR009057. Homeodomain-like.
IPR002197. HTH_Fis.
IPR027417. P-loop_NTPase.
IPR000014. PAS.
IPR013767. PAS_fold.
IPR002078. Sigma_54_int.
IPR025662. Sigma_54_int_dom_ATP-bd_1.
IPR025943. Sigma_54_int_dom_ATP-bd_2.
[Graphical view]
PfamiPF02954. HTH_8. 1 hit.
PF00989. PAS. 1 hit.
PF00158. Sigma54_activat. 1 hit.
[Graphical view]
SMARTiSM00382. AAA. 1 hit.
SM00091. PAS. 1 hit.
[Graphical view]
SUPFAMiSSF46689. SSF46689. 1 hit.
SSF52540. SSF52540. 1 hit.
SSF55785. SSF55785. 1 hit.
PROSITEiPS00675. SIGMA54_INTERACT_1. 1 hit.
PS00676. SIGMA54_INTERACT_2. 1 hit.
PS50045. SIGMA54_INTERACT_4. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiDHAR_ECOLI
AccessioniPrimary (citable) accession number: P76016
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: December 1, 2000
Last modified: November 2, 2016
This is version 135 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Miscellaneous

DhaK and DhaL-ADP bind to DhaR in a mutually exclusive manner, and act as corepressor and coactivator of DhaR, respectively. DhaK binds to the sensor domain of DhaR and keeps it in a transcription inactive state. In the presence of dihydroxyacetone, DhaR activity is activated by DhaL-ADP: DhaK that binds dihydroxyacetone has less affinity for DhaR and is displaced by DhaL-ADP, which activates DhaR.

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.