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Protein

Blue light- and temperature-regulated antirepressor BluF

Gene

bluF

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Binds to and releases the BluR repressor from its bound DNA target in a blue light-dependent (470 nm) fashion. A shift to low temperature also triggers a BluF-mediated relief of repression by BluR, suggesting BluF may serve as a thermometer. Blue light may act to increase the affinity of BluF for BluR, allowing it to be released from its operator. The protein has a reversible photocycle, and undergoes structural changes, probably in the EAL domain, in response to light.4 Publications

Cofactori

FAD1 PublicationNote: Binds 1 FAD per subunit, non-covalently bound to the BLUF domain.1 Publication

GO - Molecular functioni

  • blue light photoreceptor activity Source: InterPro
  • FAD binding Source: InterPro
  • flavin adenine dinucleotide binding Source: EcoCyc
  • repressing transcription factor binding Source: EcoCyc

GO - Biological processi

  • negative regulation of sequence-specific DNA binding transcription factor activity Source: EcoCyc
  • protein-chromophore linkage Source: UniProtKB-KW
  • response to blue light Source: EcoCyc
Complete GO annotation...

Keywords - Molecular functioni

Photoreceptor protein, Receptor

Keywords - Biological processi

Sensory transduction

Keywords - Ligandi

Chromophore, FAD, Flavoprotein

Enzyme and pathway databases

BioCyciEcoCyc:G6603-MONOMER.
ECOL316407:JW1150-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Blue light- and temperature-regulated antirepressor BluF
Short name:
Blrp
Gene namesi
Name:bluF1 Publication
Synonyms:blrp1 Publication, ycgF
Ordered Locus Names:b1163, JW1150
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG13887. bluF.

Pathology & Biotechi

Disruption phenotypei

Reduces expression of ycgZ in the presence but not the absence of BluR. At 16 degrees Celsius, reduces expression of genes (including ycgZ) for several small proteins. While the BluR/F system is induced at low temperatures under blue light irradiation, it is not essential for growth under these conditions.1 Publication

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi193 – 1953IVQ → LVR: Does not confer c-di-GMP phosphodiesterase activity. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 403403Blue light- and temperature-regulated antirepressor BluFPRO_0000168852Add
BLAST

Proteomic databases

EPDiP75990.
PaxDbiP75990.
PRIDEiP75990.

Expressioni

Inductioni

Expression is strongly activated by cold shock (over 30-fold at 16 degrees Celsius compared to 37 degrees) at low temperature in a PNPase-dependent fashion. Repressed by RpoS.2 Publications

Interactioni

Subunit structurei

Monomer, it undergoes transient dimerization following photoexcitation or upon temperature reduction, with a relaxation time of about 2 minutes. The dimer may be the inactive state. Interacts with the N- and C-terminal domains of BluR. Can also interact with the C-terminal domain of MlrA.5 Publications

GO - Molecular functioni

  • repressing transcription factor binding Source: EcoCyc

Protein-protein interaction databases

BioGridi4262864. 21 interactions.
DIPiDIP-11553N.
IntActiP75990. 13 interactions.
STRINGi511145.b1163.

Structurei

3D structure databases

ProteinModelPortaliP75990.
SMRiP75990. Positions 1-400.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini2 – 9392BLUFPROSITE-ProRule annotationAdd
BLAST
Domaini155 – 403249EALPROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni98 – 14447Joining helixAdd
BLAST

Domaini

The joining helix is required for stability of the light-adapted state.1 Publication
Contains a defective C-terminal cyclic-di-GMP phosphodiesterase EAL domain, which lacks key amino acids required for phosphodiesterase activity. Restoration of consensus amino acids in the degenerate EAL domain does not restore phosphodiesterase activity and reduces ability to antagonize BluR.1 Publication

Sequence similaritiesi

Contains 1 BLUF domain.PROSITE-ProRule annotation
Contains 1 EAL domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiENOG4105BZU. Bacteria.
ENOG410XNMH. LUCA.
HOGENOMiHOG000119657.
InParanoidiP75990.
OMAiGISQFQG.
PhylomeDBiP75990.

Family and domain databases

CDDicd01948. EAL. 1 hit.
Gene3Di3.20.20.450. 1 hit.
InterProiIPR001792. Acylphosphatase-like_dom.
IPR007024. BLUF_domain.
IPR001633. EAL_dom.
[Graphical view]
PfamiPF04940. BLUF. 1 hit.
PF00563. EAL. 1 hit.
[Graphical view]
SMARTiSM01034. BLUF. 1 hit.
SM00052. EAL. 1 hit.
[Graphical view]
SUPFAMiSSF141868. SSF141868. 1 hit.
SSF54975. SSF54975. 1 hit.
PROSITEiPS50925. BLUF. 1 hit.
PS50883. EAL. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P75990-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLTTLIYRSH IRDDEPVKKI EEMVSIANRR NMQSDVTGIL LFNGSHFFQL
60 70 80 90 100
LEGPEEQVKM IYRAICQDPR HYNIVELLCD YAPARRFGKA GMELFDLRLH
110 120 130 140 150
ERDDVLQAVF DKGTSKFQLT YDDRALQFFR TFVLATEQST YFEIPAEDSW
160 170 180 190 200
LFIADGSDKE LDSCALSPTI NDHFAFHPIV DPLSRRIIAF EAIVQKNEDS
210 220 230 240 250
PSAIAVGQRK DGEIYTADLK SKALAFTMAH ALELGDKMIS INLLPMTLVN
260 270 280 290 300
EPDAVSFLLN EIKANALVPE QIIVEFTESE VISRFDEFAE AIKSLKAAGI
310 320 330 340 350
SVAIDHFGAG FAGLLLLSRF QPDRIKISQE LITNVHKSGP RQAIIQAIIK
360 370 380 390 400
CCTSLEIQVS AMGVATPEEW MWLESAGIEM FQGDLFAKAK LNGIPSIAWP

EKK
Length:403
Mass (Da):45,295
Last modified:February 1, 1997 - v1
Checksum:i57B662BEC10957DA
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00096 Genomic DNA. Translation: AAC74247.1.
AP009048 Genomic DNA. Translation: BAA35998.1.
PIRiH64861.
RefSeqiNP_415681.1. NC_000913.3.
WP_001299269.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC74247; AAC74247; b1163.
BAA35998; BAA35998; BAA35998.
GeneIDi947592.
KEGGiecj:JW1150.
eco:b1163.
PATRICi32117563. VBIEscCol129921_1204.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00096 Genomic DNA. Translation: AAC74247.1.
AP009048 Genomic DNA. Translation: BAA35998.1.
PIRiH64861.
RefSeqiNP_415681.1. NC_000913.3.
WP_001299269.1. NZ_LN832404.1.

3D structure databases

ProteinModelPortaliP75990.
SMRiP75990. Positions 1-400.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4262864. 21 interactions.
DIPiDIP-11553N.
IntActiP75990. 13 interactions.
STRINGi511145.b1163.

Proteomic databases

EPDiP75990.
PaxDbiP75990.
PRIDEiP75990.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC74247; AAC74247; b1163.
BAA35998; BAA35998; BAA35998.
GeneIDi947592.
KEGGiecj:JW1150.
eco:b1163.
PATRICi32117563. VBIEscCol129921_1204.

Organism-specific databases

EchoBASEiEB3646.
EcoGeneiEG13887. bluF.

Phylogenomic databases

eggNOGiENOG4105BZU. Bacteria.
ENOG410XNMH. LUCA.
HOGENOMiHOG000119657.
InParanoidiP75990.
OMAiGISQFQG.
PhylomeDBiP75990.

Enzyme and pathway databases

BioCyciEcoCyc:G6603-MONOMER.
ECOL316407:JW1150-MONOMER.

Miscellaneous databases

PROiP75990.

Family and domain databases

CDDicd01948. EAL. 1 hit.
Gene3Di3.20.20.450. 1 hit.
InterProiIPR001792. Acylphosphatase-like_dom.
IPR007024. BLUF_domain.
IPR001633. EAL_dom.
[Graphical view]
PfamiPF04940. BLUF. 1 hit.
PF00563. EAL. 1 hit.
[Graphical view]
SMARTiSM01034. BLUF. 1 hit.
SM00052. EAL. 1 hit.
[Graphical view]
SUPFAMiSSF141868. SSF141868. 1 hit.
SSF54975. SSF54975. 1 hit.
PROSITEiPS50925. BLUF. 1 hit.
PS50883. EAL. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiBLUF_ECOLI
AccessioniPrimary (citable) accession number: P75990
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 27, 2001
Last sequence update: February 1, 1997
Last modified: September 7, 2016
This is version 117 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.