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Protein

HTH-type transcriptional repressor BluR

Gene

bluR

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Controls the expression of several small proteins that may play a role in biofilm maturation. Binds to and represses the operator of the ycgZ-ymgA-ariR-ymgC operon and also regulates ynaK. Binding is antagonized by BluF upon blue light (470 nm) irradiation. Blue light may increase the affinity of BluF for BluR, allowing it to be released from its operator.2 Publications

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
DNA bindingi6 – 25H-T-H motifPROSITE-ProRule annotationAdd BLAST20

GO - Molecular functioni

  • DNA binding Source: UniProtKB-KW
  • transcription factor activity, sequence-specific DNA binding Source: EcoCyc

GO - Biological processi

Keywordsi

Molecular functionDNA-binding, Repressor
Biological processTranscription, Transcription regulation

Enzyme and pathway databases

BioCyciEcoCyc:G6602-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
HTH-type transcriptional repressor BluRCurated
Gene namesi
Name:bluR1 Publication
Synonyms:ycgE
Ordered Locus Names:b1162, JW1149
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG13886. bluR.

Pathology & Biotechi

Disruption phenotypei

3- to 20-fold derepression of several genes coding for small proteins including the ycgZ-ymgA-ariR-ymgC operon. While the BluR/F system is induced at low temperatures under blue light irradiation, it is not essential for growth under these conditions. When both lon and bluR are disrupted, levels of OmpF decrease, leading to lower drug susceptibility, with a greater effect at 26 degrees than at 37 degrees Celsius. The mechanism is not yet understood.2 Publications

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000981651 – 243HTH-type transcriptional repressor BluRAdd BLAST243

Proteomic databases

EPDiP75989.
PaxDbiP75989.
PRIDEiP75989.

Expressioni

Inductioni

Expression is strongly activated (7-fold at 16 degrees Celsius compared to 37 degrees) at low temperature.1 Publication

Interactioni

Subunit structurei

Interacts with BluF.2 Publications

Protein-protein interaction databases

BioGridi4260084. 6 interactors.
DIPiDIP-11552N.
IntActiP75989. 39 interactors.
MINTiMINT-1244002.
STRINGi316385.ECDH10B_1211.

Structurei

3D structure databases

ProteinModelPortaliP75989.
SMRiP75989.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini3 – 72HTH merR-typePROSITE-ProRule annotationAdd BLAST70

Phylogenomic databases

eggNOGiENOG4108XDN. Bacteria.
COG0789. LUCA.
HOGENOMiHOG000119656.
InParanoidiP75989.

Family and domain databases

InterProiView protein in InterPro
IPR009061. DNA-bd_dom_put.
IPR000551. MerR-type_HTH_dom.
PfamiView protein in Pfam
PF13411. MerR_1. 1 hit.
SMARTiView protein in SMART
SM00422. HTH_MERR. 1 hit.
SUPFAMiSSF46955. SSF46955. 1 hit.
PROSITEiView protein in PROSITE
PS00552. HTH_MERR_1. 1 hit.
PS50937. HTH_MERR_2. 1 hit.

Sequencei

Sequence statusi: Complete.

P75989-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAYYSIGDVA ERCGINPVTL RAWQRRYGLL KPQRSEGGHR LFDEEDIQRI
60 70 80 90 100
EEIKRWISNG VPVGKVKALL ETTSQDTEDD WSRLQEEMMS ILRMANPAKL
110 120 130 140 150
RARIISLGRE YPVDQLINHV YLPVRQRLVL DHNTSRIMSS MFDGALIEYA
160 170 180 190 200
ATSLFEMRRK PGKEAILMAW NVEERARLWL EAWRLSLSGW HISVLADPIE
210 220 230 240
SPRPELFPTQ TLIVWTGMAP TRRQNELLQH WGEQGYKVIF HAP
Length:243
Mass (Da):28,265
Last modified:February 1, 1997 - v1
Checksum:iE8E5AD376A18EB84
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00096 Genomic DNA. Translation: AAC74246.1.
AP009048 Genomic DNA. Translation: BAA35997.1.
PIRiG64861.
RefSeqiNP_415680.1. NC_000913.3.
WP_000332303.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC74246; AAC74246; b1162.
BAA35997; BAA35997; BAA35997.
GeneIDi945735.
KEGGiecj:JW1149.
eco:b1162.
PATRICifig|1411691.4.peg.1128.

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

Entry informationi

Entry nameiBLUR_ECOLI
AccessioniPrimary (citable) accession number: P75989
Secondary accession number(s): Q9R7N5
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: February 1, 1997
Last modified: July 5, 2017
This is version 135 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene