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Protein

Ribosomal large subunit pseudouridine synthase E

Gene

rluE

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Responsible for synthesis of pseudouridine from uracil-2457 in 23S ribosomal RNA.1 Publication

Catalytic activityi

23S rRNA uridine(2457) = 23S rRNA pseudouridine(2457).1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei79Nucleophile1 Publication1

GO - Molecular functioni

  • pseudouridine synthase activity Source: EcoCyc
  • RNA binding Source: InterPro

GO - Biological processi

  • enzyme-directed rRNA pseudouridine synthesis Source: EcoCyc
Complete GO annotation...

Keywords - Molecular functioni

Isomerase

Keywords - Biological processi

rRNA processing

Enzyme and pathway databases

BioCyciEcoCyc:G6581-MONOMER.
ECOL316407:JW1121-MONOMER.
MetaCyc:G6581-MONOMER.
BRENDAi5.4.99.20. 2026.

Names & Taxonomyi

Protein namesi
Recommended name:
Ribosomal large subunit pseudouridine synthase E (EC:5.4.99.20)
Alternative name(s):
rRNA pseudouridylate synthase E
rRNA-uridine isomerase E
Gene namesi
Name:rluE
Synonyms:ymfC
Ordered Locus Names:b1135, JW1121
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG13447. rluE.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi79D → N or T: Loss of activity. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001000011 – 217Ribosomal large subunit pseudouridine synthase EAdd BLAST217

Proteomic databases

PaxDbiP75966.
PRIDEiP75966.

Interactioni

Protein-protein interaction databases

BioGridi4263127. 17 interactors.
DIPiDIP-12716N.
IntActiP75966. 8 interactors.
STRINGi511145.b1135.

Structurei

Secondary structure

1217
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi41 – 46Combined sources6
Helixi63 – 65Combined sources3
Beta strandi73 – 76Combined sources4
Beta strandi83 – 90Combined sources8
Helixi92 – 99Combined sources8
Beta strandi107 – 115Combined sources9
Helixi119 – 127Combined sources9
Beta strandi140 – 144Combined sources5
Beta strandi165 – 173Combined sources9
Helixi179 – 186Combined sources8
Beta strandi191 – 199Combined sources9
Beta strandi212 – 214Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2OLWX-ray1.60A/B1-217[»]
2OMLX-ray1.20A29-217[»]
ProteinModelPortaliP75966.
SMRiP75966.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP75966.

Family & Domainsi

Sequence similaritiesi

Belongs to the pseudouridine synthase RsuA family.Curated

Phylogenomic databases

eggNOGiENOG4108R99. Bacteria.
COG1187. LUCA.
HOGENOMiHOG000044955.
InParanoidiP75966.
KOiK06181.
OMAiFRKHRVE.
PhylomeDBiP75966.

Family and domain databases

InterProiIPR020103. PsdUridine_synth_cat_dom.
IPR000748. PsdUridine_synth_RsuA/RluB/E/F.
IPR018496. PsdUridine_synth_RsuA/RluB_CS.
IPR006145. PsdUridine_synth_RsuA/RluD.
[Graphical view]
PfamiPF00849. PseudoU_synth_2. 1 hit.
[Graphical view]
SUPFAMiSSF55120. SSF55120. 1 hit.
TIGRFAMsiTIGR00093. TIGR00093. 1 hit.
PROSITEiPS01149. PSI_RSU. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P75966-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRQFIISENT MQKTSFRNHQ VKRFSSQRST RRKPENQPTR VILFNKPYDV
60 70 80 90 100
LPQFTDEAGR KTLKEFIPVQ GVYAAGRLDR DSEGLLVLTN NGALQARLTQ
110 120 130 140 150
PGKRTGKIYY VQVEGIPTQD ALEALRNGVT LNDGPTLPAG AELVDEPAWL
160 170 180 190 200
WPRNPPIRER KSIPTSWLKI TLYEGRNRQV RRMTAHVGFP TLRLIRYAMG
210
DYSLDNLANG EWREVTD
Length:217
Mass (Da):24,880
Last modified:December 15, 1998 - v2
Checksum:iF7C7A7CEDC5FD3F6
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00096 Genomic DNA. Translation: AAC74219.2.
AP009048 Genomic DNA. Translation: BAA35957.1.
PIRiD64858.
RefSeqiNP_415653.4. NC_000913.3.
WP_001248691.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC74219; AAC74219; b1135.
BAA35957; BAA35957; BAA35957.
GeneIDi945701.
KEGGiecj:JW1121.
eco:b1135.
PATRICi32117519. VBIEscCol129921_1182.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00096 Genomic DNA. Translation: AAC74219.2.
AP009048 Genomic DNA. Translation: BAA35957.1.
PIRiD64858.
RefSeqiNP_415653.4. NC_000913.3.
WP_001248691.1. NZ_LN832404.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2OLWX-ray1.60A/B1-217[»]
2OMLX-ray1.20A29-217[»]
ProteinModelPortaliP75966.
SMRiP75966.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4263127. 17 interactors.
DIPiDIP-12716N.
IntActiP75966. 8 interactors.
STRINGi511145.b1135.

Proteomic databases

PaxDbiP75966.
PRIDEiP75966.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC74219; AAC74219; b1135.
BAA35957; BAA35957; BAA35957.
GeneIDi945701.
KEGGiecj:JW1121.
eco:b1135.
PATRICi32117519. VBIEscCol129921_1182.

Organism-specific databases

EchoBASEiEB3221.
EcoGeneiEG13447. rluE.

Phylogenomic databases

eggNOGiENOG4108R99. Bacteria.
COG1187. LUCA.
HOGENOMiHOG000044955.
InParanoidiP75966.
KOiK06181.
OMAiFRKHRVE.
PhylomeDBiP75966.

Enzyme and pathway databases

BioCyciEcoCyc:G6581-MONOMER.
ECOL316407:JW1121-MONOMER.
MetaCyc:G6581-MONOMER.
BRENDAi5.4.99.20. 2026.

Miscellaneous databases

EvolutionaryTraceiP75966.
PROiP75966.

Family and domain databases

InterProiIPR020103. PsdUridine_synth_cat_dom.
IPR000748. PsdUridine_synth_RsuA/RluB/E/F.
IPR018496. PsdUridine_synth_RsuA/RluB_CS.
IPR006145. PsdUridine_synth_RsuA/RluD.
[Graphical view]
PfamiPF00849. PseudoU_synth_2. 1 hit.
[Graphical view]
SUPFAMiSSF55120. SSF55120. 1 hit.
TIGRFAMsiTIGR00093. TIGR00093. 1 hit.
PROSITEiPS01149. PSI_RSU. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiRLUE_ECOLI
AccessioniPrimary (citable) accession number: P75966
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: December 15, 1998
Last modified: November 2, 2016
This is version 123 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.