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Protein

Ribosomal large subunit pseudouridine synthase E

Gene

rluE

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Responsible for synthesis of pseudouridine from uracil-2457 in 23S ribosomal RNA.1 Publication

Catalytic activityi

23S rRNA uridine(2457) = 23S rRNA pseudouridine(2457).1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei79Nucleophile1 Publication1

GO - Molecular functioni

  • pseudouridine synthase activity Source: EcoCyc
  • RNA binding Source: InterPro

GO - Biological processi

  • enzyme-directed rRNA pseudouridine synthesis Source: EcoCyc

Keywordsi

Molecular functionIsomerase
Biological processrRNA processing

Enzyme and pathway databases

BioCyciEcoCyc:G6581-MONOMER
MetaCyc:G6581-MONOMER
BRENDAi5.4.99.20 2026

Names & Taxonomyi

Protein namesi
Recommended name:
Ribosomal large subunit pseudouridine synthase E (EC:5.4.99.20)
Alternative name(s):
rRNA pseudouridylate synthase E
rRNA-uridine isomerase E
Gene namesi
Name:rluE
Synonyms:ymfC
Ordered Locus Names:b1135, JW1121
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG13447 rluE

Subcellular locationi

GO - Cellular componenti

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi79D → N or T: Loss of activity. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001000011 – 217Ribosomal large subunit pseudouridine synthase EAdd BLAST217

Proteomic databases

PaxDbiP75966
PRIDEiP75966

Interactioni

Protein-protein interaction databases

BioGridi4263127, 32 interactors
DIPiDIP-12716N
IntActiP75966, 8 interactors
STRINGi316385.ECDH10B_1207

Structurei

Secondary structure

1217
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi41 – 46Combined sources6
Helixi63 – 65Combined sources3
Beta strandi73 – 76Combined sources4
Beta strandi83 – 90Combined sources8
Helixi92 – 99Combined sources8
Beta strandi107 – 115Combined sources9
Helixi119 – 127Combined sources9
Beta strandi140 – 144Combined sources5
Beta strandi165 – 173Combined sources9
Helixi179 – 186Combined sources8
Beta strandi191 – 199Combined sources9
Beta strandi212 – 214Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2OLWX-ray1.60A/B1-217[»]
2OMLX-ray1.20A29-217[»]
ProteinModelPortaliP75966
SMRiP75966
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP75966

Family & Domainsi

Sequence similaritiesi

Belongs to the pseudouridine synthase RsuA family.Curated

Phylogenomic databases

eggNOGiENOG4108R99 Bacteria
COG1187 LUCA
HOGENOMiHOG000044955
InParanoidiP75966
KOiK06181
OMAiRTYWVQV
PhylomeDBiP75966

Family and domain databases

InterProiView protein in InterPro
IPR020103 PsdUridine_synth_cat_dom_sf
IPR000748 PsdUridine_synth_RsuA/RluB/E/F
IPR018496 PsdUridine_synth_RsuA/RluB_CS
IPR006145 PsdUridine_synth_RsuA/RluD
PANTHERiPTHR21600 PTHR21600, 1 hit
PfamiView protein in Pfam
PF00849 PseudoU_synth_2, 1 hit
SUPFAMiSSF55120 SSF55120, 1 hit
TIGRFAMsiTIGR00093 TIGR00093, 1 hit
PROSITEiView protein in PROSITE
PS01149 PSI_RSU, 1 hit

Sequencei

Sequence statusi: Complete.

P75966-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRQFIISENT MQKTSFRNHQ VKRFSSQRST RRKPENQPTR VILFNKPYDV
60 70 80 90 100
LPQFTDEAGR KTLKEFIPVQ GVYAAGRLDR DSEGLLVLTN NGALQARLTQ
110 120 130 140 150
PGKRTGKIYY VQVEGIPTQD ALEALRNGVT LNDGPTLPAG AELVDEPAWL
160 170 180 190 200
WPRNPPIRER KSIPTSWLKI TLYEGRNRQV RRMTAHVGFP TLRLIRYAMG
210
DYSLDNLANG EWREVTD
Length:217
Mass (Da):24,880
Last modified:December 15, 1998 - v2
Checksum:iF7C7A7CEDC5FD3F6
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00096 Genomic DNA Translation: AAC74219.2
AP009048 Genomic DNA Translation: BAA35957.1
PIRiD64858
RefSeqiNP_415653.4, NC_000913.3
WP_001248691.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaiAAC74219; AAC74219; b1135
BAA35957; BAA35957; BAA35957
GeneIDi945701
KEGGiecj:JW1121
eco:b1135
PATRICifig|1411691.4.peg.1131

Similar proteinsi

Entry informationi

Entry nameiRLUE_ECOLI
AccessioniPrimary (citable) accession number: P75966
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: December 15, 1998
Last modified: March 28, 2018
This is version 131 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

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