P75962 (YMFA_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Inner membrane protein YmfA

Gene names

Name:	ymfA
Ordered Locus Names:	b1122, JW5164

Organism

Escherichia coli (strain K12) [Reference proteome] [HAMAP]

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia › Escherichia coli

Protein attributes

Sequence length	153 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Subcellular location

Cell inner membrane; Multi-pass membrane protein.

Ontologies

Keywords
Cellular component	Cell inner membrane Cell membrane Membrane
Domain	Transmembrane Transmembrane helix
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	cellular component movement Inferred from mutant phenotype PubMed 17122336. Source: EcoCyc
Cellular_component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 153	153	Inner membrane protein YmfA		PRO_0000168840
Regions
	Topological domain	1 – 6	6	Cytoplasmic Potential
	Transmembrane	7 – 27	21	Helical; Potential
	Topological domain	28 – 116	89	Periplasmic Potential
	Transmembrane	117 – 137	21	Helical; Potential
	Topological domain	138 – 153	16	Cytoplasmic Potential

Sequences

	Sequence		Length	Mass (Da)
	P75962 [UniParc]. Last modified August 29, 2001. Version 2. Checksum: DFD1327C9505EB33	FASTA	153	17,416
	10 20 30 40 50 60 MSQDSKVFFR IFLGIGLVLI LISVVVFYNQ FTYSKDAIHT EGVIVDTVWH SSHSHRTGKD 70 80 90 100 110 120 GSWYPVVAFR PTPDYTLIFN SSIGSDFYED SEGDKVNVYY SPGHPEKAEI NNPWVNFFKW 130 140 150 GFIGIMGVIF IAVGLLISMP SSKKSRRKRK SRP

References

[1]	"A 718-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 12.7-28.0 min region on the linkage map." Oshima T., Aiba H., Baba T., Fujita K., Hayashi K., Honjo A., Ikemoto K., Inada T., Itoh T., Kajihara M., Kanai K., Kashimoto K., Kimura S., Kitagawa M., Makino K., Masuda S., Miki T., Mizobuchi K. , Mori H., Motomura K., Nakamura Y., Nashimoto H., Nishio Y., Saito N., Sampei G., Seki Y., Tagami H., Takemoto K., Wada C., Yamamoto Y., Yano M., Horiuchi T. DNA Res. 3:137-155(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[2]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[3]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[4]	"Global topology analysis of the Escherichia coli inner membrane proteome." Daley D.O., Rapp M., Granseth E., Melen K., Drew D., von Heijne G. Science 308:1321-1323(2005) [PubMed] [Europe PMC] [Abstract] Cited for: TOPOLOGY [LARGE SCALE ANALYSIS]. Strain: K12 / MG1655 / ATCC 47076.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U00096 Genomic DNA. Translation: AAC74206.2. AP009048 Genomic DNA. Translation: BAA35942.2.
PIR	G64856.
RefSeq	NP_415640.2. NC_000913.2. YP_489390.1. NC_007779.1.
3D structure databases
ProteinModelPortal	P75962.
ModBase	Search...
Protein-protein interaction databases
STRING	511145.b1122.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAC74206; AAC74206; b1122. BAA35942; BAA35942; BAA35942.
GeneID	12931097. 945684.
KEGG	ecj:Y75_p1092. eco:b1122.
PATRIC	32117491. VBIEscCol129921_1168.
Organism-specific databases
EchoBASE	EB3219.
EcoGene	EG13445. ymfA.
Phylogenomic databases
eggNOG	NOG328419.
HOGENOM	HOG000119107.
OMA	RKRKSCP.
ProtClustDB	CLSK908107.
Enzyme and pathway databases
BioCyc	EcoCyc:G6579-MONOMER. ECOL316407:JW5164-MONOMER.
Gene expression databases
Genevestigator	P75962.
Family and domain databases
InterPro	IPR021994. DUF3592. [Graphical view]
Pfam	PF12158. DUF3592. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

YMFA_ECOLI

Accession

Primary (citable) accession number: P75962

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1998
Last sequence update:	August 29, 2001
Last modified:	May 1, 2013
This is version 82 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program