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Protein

N-acetyl-D-glucosamine kinase

Gene

nagK

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes the phosphorylation of N-acetyl-D-glucosamine (GlcNAc) derived from cell-wall degradation, yielding GlcNAc-6-P. Has also low level glucokinase activity in vitro.1 Publication

Catalytic activityi

ATP + N-acetyl-D-glucosamine = ADP + N-acetyl-D-glucosamine 6-phosphate.1 Publication

Enzyme regulationi

Strongly inhibited by ADP.1 Publication

Kineticsi

  1. KM=342 µM for GlcNAc (at 37 degrees Celsius and pH 7.5)2 Publications
  2. KM=896 µM for ATP (at 37 degrees Celsius and pH 7.5)2 Publications
  3. KM=37 mM for glucose (at 37 degrees Celsius and pH 7.5)2 Publications
  4. KM=3.4 mM for ATP (at 25 degrees Celsius and pH 7.6)2 Publications
  5. KM=3.8 mM for glucose (at 25 degrees Celsius and pH 7.6)2 Publications
  1. Vmax=118 µmol/min/mg enzyme with GlcNAc as substrate (at 37 degrees Celsius and pH 7.5)2 Publications
  2. Vmax=24 µmol/min/mg enzyme with glucose as substrate (at 37 degrees Celsius and pH 7.5)2 Publications

pH dependencei

Active from pH 6.5 to 10.1 Publication

Pathwayi: peptidoglycan recycling

This protein is involved in the pathway peptidoglycan recycling, which is part of Cell wall biogenesis.
View all proteins of this organism that are known to be involved in the pathway peptidoglycan recycling and in Cell wall biogenesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi157ZincBy similarity1
Metal bindingi177ZincBy similarity1
Metal bindingi179ZincBy similarity1
Metal bindingi184ZincBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi4 – 11ATPSequence analysis8
Nucleotide bindingi133 – 140ATPSequence analysis8

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • metal ion binding Source: UniProtKB-KW
  • N-acetylglucosamine kinase activity Source: EcoCyc

GO - Biological processi

Keywordsi

Molecular functionKinase, Transferase
Biological processCarbohydrate metabolism
LigandATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciEcoCyc:G6576-MONOMER
MetaCyc:G6576-MONOMER
SABIO-RKP75959
UniPathwayiUPA00544

Names & Taxonomyi

Protein namesi
Recommended name:
N-acetyl-D-glucosamine kinase (EC:2.7.1.59)
Alternative name(s):
GlcNAc kinase
Gene namesi
Name:nagK
Synonyms:ycfX
Ordered Locus Names:b1119, JW1105
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG13442 nagK

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000957201 – 303N-acetyl-D-glucosamine kinaseAdd BLAST303

Proteomic databases

PaxDbiP75959
PRIDEiP75959

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
zntRP0ACS52EBI-556240,EBI-562184

Protein-protein interaction databases

BioGridi4260083, 10 interactors
DIPiDIP-11548N
IntActiP75959, 10 interactors
STRINGi316385.ECDH10B_1191

Structurei

3D structure databases

ProteinModelPortaliP75959
SMRiP75959
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the ROK (NagC/XylR) family. NagK subfamily.Curated

Phylogenomic databases

eggNOGiENOG4105EZX Bacteria
COG1940 LUCA
HOGENOMiHOG000150087
InParanoidiP75959
KOiK00884
OMAiLQLNYHA
PhylomeDBiP75959

Family and domain databases

HAMAPiMF_01271 GlcNAc_kinase, 1 hit
InterProiView protein in InterPro
IPR023505 N-acetyl-D-glucosamine_kinase
IPR000600 ROK
PANTHERiPTHR18964 PTHR18964, 1 hit
PfamiView protein in Pfam
PF00480 ROK, 1 hit
PROSITEiView protein in PROSITE
PS01125 ROK, 1 hit

Sequencei

Sequence statusi: Complete.

P75959-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MYYGFDIGGT KIALGVFDSG RQLQWEKRVP TPRDSYDAFL DAVCELVAEA
60 70 80 90 100
DQRFGCKGSV GIGIPGMPET EDGTLYAANV PAASGKPLRA DLSARLDRDV
110 120 130 140 150
RLDNDANCFA LSEAWDDEFT QYPLVMGLIL GTGVGGGLIF NGKPITGKSY
160 170 180 190 200
ITGEFGHMRL PVDALTMMGL DFPLRRCGCG QHGCIENYLS GRGFAWLYQH
210 220 230 240 250
YYHQPLQAPE IIALYDQGDE QARAHVERYL DLLAVCLGNI LTIVDPDLVV
260 270 280 290 300
IGGGLSNFPA ITTQLADRLP RHLLPVARVP RIERARHGDA GGMRGAAFLH

LTD
Length:303
Mass (Da):33,043
Last modified:February 1, 1997 - v1
Checksum:iA857E63925894BBD
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00096 Genomic DNA Translation: AAC74203.1
AP009048 Genomic DNA Translation: BAA35939.1
PIRiD64856
RefSeqiNP_415637.1, NC_000913.3
WP_000291270.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaiAAC74203; AAC74203; b1119
BAA35939; BAA35939; BAA35939
GeneIDi945664
KEGGiecj:JW1105
eco:b1119
PATRICifig|1411691.4.peg.1148

Similar proteinsi

Entry informationi

Entry nameiNAGK_ECOLI
AccessioniPrimary (citable) accession number: P75959
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: February 1, 1997
Last modified: March 28, 2018
This is version 120 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

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