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Protein

Lipoprotein-releasing system ATP-binding protein LolD

Gene

lolD

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Part of the ABC transporter complex LolCDE involved in the translocation of mature outer membrane-directed lipoproteins, from the inner membrane to the periplasmic chaperone, LolA. Responsible for the formation of the LolA-lipoprotein complex in an ATP-dependent manner. Such a release is dependent of the sorting-signal (absence of an Asp at position 2 of the mature lipoprotein) and of LolA.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi42 – 498ATPUniRule annotation

GO - Molecular functioni

  • ATPase activity Source: InterPro
  • ATP binding Source: UniProtKB-HAMAP
  • lipoprotein transporter activity Source: EcoCyc

GO - Biological processi

  • lipoprotein localization to outer membrane Source: CACAO
  • lipoprotein transport Source: EcoCyc
  • protein localization to outer membrane Source: CACAO
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Transport

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciEcoCyc:YCFV-MONOMER.
ECOL316407:JW5162-MONOMER.

Protein family/group databases

TCDBi3.A.1.125.1. the atp-binding cassette (abc) superfamily.

Names & Taxonomyi

Protein namesi
Recommended name:
Lipoprotein-releasing system ATP-binding protein LolDUniRule annotation (EC:3.6.3.-UniRule annotation)
Gene namesi
Name:lolDUniRule annotation
Synonyms:ycfV
Ordered Locus Names:b1117, JW5162
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG13440. lolD.

Subcellular locationi

GO - Cellular componenti

  • ATP-binding cassette (ABC) transporter complex Source: UniProtKB-HAMAP
  • plasma membrane Source: CACAO
  • protein complex Source: EcoCyc
Complete GO annotation...

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi42 – 421G → D: Loss of lipoprotein release when overexpressed. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 233233Lipoprotein-releasing system ATP-binding protein LolDPRO_0000092434Add
BLAST

Proteomic databases

EPDiP75957.
PaxDbiP75957.
PRIDEiP75957.

Interactioni

Subunit structurei

The complex is composed of two ATP-binding proteins (LolD) and two transmembrane proteins (LolC and LolE).UniRule annotation1 Publication

Protein-protein interaction databases

BioGridi4260088. 187 interactions.
DIPiDIP-10115N.
IntActiP75957. 7 interactions.
STRINGi511145.b1117.

Structurei

3D structure databases

ProteinModelPortaliP75957.
SMRiP75957. Positions 23-228.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini6 – 233228ABC transporterUniRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the ABC transporter superfamily. Lipoprotein translocase (TC 3.A.1.125) family. [View classification]UniRule annotation
Contains 1 ABC transporter domain.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105CQU. Bacteria.
COG1136. LUCA.
InParanoidiP75957.
KOiK09810.
OMAiAMQPGEM.
PhylomeDBiP75957.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR003593. AAA+_ATPase.
IPR003439. ABC_transporter-like.
IPR017871. ABC_transporter_CS.
IPR015854. ABC_transpr_LolD.
IPR011924. LolD_lipo_ATP-bd.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF00005. ABC_tran. 1 hit.
[Graphical view]
SMARTiSM00382. AAA. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR02211. LolD_lipo_ex. 1 hit.
PROSITEiPS00211. ABC_TRANSPORTER_1. 1 hit.
PS50893. ABC_TRANSPORTER_2. 1 hit.
PS51244. LOLD. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P75957-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNKILLQCDN LCKRYQEGSV QTDVLHNVSF SVGEGEMMAI VGSSGSGKST
60 70 80 90 100
LLHLLGGLDT PTSGDVIFNG QPMSKLSSAA KAELRNQKLG FIYQFHHLLP
110 120 130 140 150
DFTALENVAM PLLIGKKKPA EINSRALEML KAVGLDHRAN HRPSELSGGE
160 170 180 190 200
RQRVAIARAL VNNPRLVLAD EPTGNLDARN ADSIFQLLGE LNRLQGTAFL
210 220 230
VVTHDLQLAK RMSRQLEMRD GRLTAELSLM GAE
Length:233
Mass (Da):25,438
Last modified:December 15, 1998 - v2
Checksum:i1C305476182F1EFD
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00096 Genomic DNA. Translation: AAC74201.2.
AP009048 Genomic DNA. Translation: BAA35937.2.
PIRiB64856.
RefSeqiNP_415635.4. NC_000913.3.
WP_001033694.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC74201; AAC74201; b1117.
BAA35937; BAA35937; BAA35937.
GeneIDi945670.
KEGGiecj:JW5162.
eco:b1117.
PATRICi32117481. VBIEscCol129921_1163.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00096 Genomic DNA. Translation: AAC74201.2.
AP009048 Genomic DNA. Translation: BAA35937.2.
PIRiB64856.
RefSeqiNP_415635.4. NC_000913.3.
WP_001033694.1. NZ_LN832404.1.

3D structure databases

ProteinModelPortaliP75957.
SMRiP75957. Positions 23-228.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4260088. 187 interactions.
DIPiDIP-10115N.
IntActiP75957. 7 interactions.
STRINGi511145.b1117.

Protein family/group databases

TCDBi3.A.1.125.1. the atp-binding cassette (abc) superfamily.

Proteomic databases

EPDiP75957.
PaxDbiP75957.
PRIDEiP75957.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC74201; AAC74201; b1117.
BAA35937; BAA35937; BAA35937.
GeneIDi945670.
KEGGiecj:JW5162.
eco:b1117.
PATRICi32117481. VBIEscCol129921_1163.

Organism-specific databases

EchoBASEiEB3214.
EcoGeneiEG13440. lolD.

Phylogenomic databases

eggNOGiENOG4105CQU. Bacteria.
COG1136. LUCA.
InParanoidiP75957.
KOiK09810.
OMAiAMQPGEM.
PhylomeDBiP75957.

Enzyme and pathway databases

BioCyciEcoCyc:YCFV-MONOMER.
ECOL316407:JW5162-MONOMER.

Miscellaneous databases

PROiP75957.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR003593. AAA+_ATPase.
IPR003439. ABC_transporter-like.
IPR017871. ABC_transporter_CS.
IPR015854. ABC_transpr_LolD.
IPR011924. LolD_lipo_ATP-bd.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF00005. ABC_tran. 1 hit.
[Graphical view]
SMARTiSM00382. AAA. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR02211. LolD_lipo_ex. 1 hit.
PROSITEiPS00211. ABC_TRANSPORTER_1. 1 hit.
PS50893. ABC_TRANSPORTER_2. 1 hit.
PS51244. LOLD. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiLOLD_ECOLI
AccessioniPrimary (citable) accession number: P75957
Secondary accession number(s): Q9R7N6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: December 15, 1998
Last modified: September 7, 2016
This is version 132 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.