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Protein

Lipoprotein-releasing system ATP-binding protein LolD

Gene

lolD

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Part of the ABC transporter complex LolCDE involved in the translocation of mature outer membrane-directed lipoproteins, from the inner membrane to the periplasmic chaperone, LolA. Responsible for the formation of the LolA-lipoprotein complex in an ATP-dependent manner. Such a release is dependent of the sorting-signal (absence of an Asp at position 2 of the mature lipoprotein) and of LolA.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi42 – 49ATPUniRule annotation8

GO - Molecular functioni

GO - Biological processi

  • lipoprotein localization to outer membrane Source: EcoCyc
  • protein localization to outer membrane Source: CACAO

Keywordsi

Molecular functionHydrolase
Biological processTransport
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciEcoCyc:YCFV-MONOMER

Protein family/group databases

TCDBi3.A.1.125.1 the atp-binding cassette (abc) superfamily

Names & Taxonomyi

Protein namesi
Recommended name:
Lipoprotein-releasing system ATP-binding protein LolDUniRule annotation (EC:3.6.3.-UniRule annotation)
Gene namesi
Name:lolDUniRule annotation
Synonyms:ycfV
Ordered Locus Names:b1117, JW5162
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG13440 lolD

Subcellular locationi

GO - Cellular componenti

  • plasma membrane Source: CACAO
  • plasma membrane protein complex Source: EcoCyc

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi42G → D: Loss of lipoprotein release when overexpressed. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000924341 – 233Lipoprotein-releasing system ATP-binding protein LolDAdd BLAST233

Proteomic databases

EPDiP75957
PaxDbiP75957
PRIDEiP75957

Interactioni

Subunit structurei

The complex is composed of two ATP-binding proteins (LolD) and two transmembrane proteins (LolC and LolE).UniRule annotation1 Publication

Protein-protein interaction databases

BioGridi4260088, 228 interactors
DIPiDIP-10115N
IntActiP75957, 7 interactors
STRINGi316385.ECDH10B_1189

Structurei

3D structure databases

ProteinModelPortaliP75957
SMRiP75957
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini6 – 233ABC transporterUniRule annotationAdd BLAST228

Sequence similaritiesi

Belongs to the ABC transporter superfamily. Lipoprotein translocase (TC 3.A.1.125) family. [View classification]UniRule annotation

Phylogenomic databases

eggNOGiENOG4105CQU Bacteria
COG1136 LUCA
InParanoidiP75957
KOiK09810
OMAiDKSEHQM
PhylomeDBiP75957

Family and domain databases

InterProiView protein in InterPro
IPR003593 AAA+_ATPase
IPR003439 ABC_transporter-like
IPR017871 ABC_transporter_CS
IPR015854 ABC_transpr_LolD
IPR011924 LolD_lipo_ATP-bd
IPR027417 P-loop_NTPase
PfamiView protein in Pfam
PF00005 ABC_tran, 1 hit
SMARTiView protein in SMART
SM00382 AAA, 1 hit
SUPFAMiSSF52540 SSF52540, 1 hit
TIGRFAMsiTIGR02211 LolD_lipo_ex, 1 hit
PROSITEiView protein in PROSITE
PS00211 ABC_TRANSPORTER_1, 1 hit
PS50893 ABC_TRANSPORTER_2, 1 hit
PS51244 LOLD, 1 hit

Sequencei

Sequence statusi: Complete.

P75957-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNKILLQCDN LCKRYQEGSV QTDVLHNVSF SVGEGEMMAI VGSSGSGKST
60 70 80 90 100
LLHLLGGLDT PTSGDVIFNG QPMSKLSSAA KAELRNQKLG FIYQFHHLLP
110 120 130 140 150
DFTALENVAM PLLIGKKKPA EINSRALEML KAVGLDHRAN HRPSELSGGE
160 170 180 190 200
RQRVAIARAL VNNPRLVLAD EPTGNLDARN ADSIFQLLGE LNRLQGTAFL
210 220 230
VVTHDLQLAK RMSRQLEMRD GRLTAELSLM GAE
Length:233
Mass (Da):25,438
Last modified:December 15, 1998 - v2
Checksum:i1C305476182F1EFD
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00096 Genomic DNA Translation: AAC74201.2
AP009048 Genomic DNA Translation: BAA35937.2
PIRiB64856
RefSeqiNP_415635.4, NC_000913.3
WP_001033694.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaiAAC74201; AAC74201; b1117
BAA35937; BAA35937; BAA35937
GeneIDi945670
KEGGiecj:JW5162
eco:b1117
PATRICifig|1411691.4.peg.1150

Similar proteinsi

Entry informationi

Entry nameiLOLD_ECOLI
AccessioniPrimary (citable) accession number: P75957
Secondary accession number(s): Q9R7N6
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: December 15, 1998
Last modified: March 28, 2018
This is version 142 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome
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Main funding by: National Institutes of Health