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Protein

Probable L,D-transpeptidase YcfS

Gene

ycfS

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Responsible, at least in part, for anchoring of the major outer membrane lipoprotein (Lpp, also known as the Braun lipoprotein) to the peptidoglycan via a meso-diaminopimelyl-L-Lys- bond on the terminal residue of Lpp.1 Publication

Pathwayi: peptidoglycan biosynthesis

This protein is involved in the pathway peptidoglycan biosynthesis, which is part of Cell wall biogenesis.
View all proteins of this organism that are known to be involved in the pathway peptidoglycan biosynthesis and in Cell wall biogenesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei217Sequence analysis1

GO - Molecular functioni

  • cysteine-type carboxypeptidase activity Source: EcoCyc
  • peptidoglycan L,D-transpeptidase activity Source: EcoCyc
  • transferase activity, transferring amino-acyl groups Source: EcoCyc
  • transferase activity, transferring glycosyl groups Source: UniProtKB-KW

GO - Biological processi

  • cell wall organization Source: UniProtKB-KW
  • Gram-negative-bacterium-type cell wall biogenesis Source: EcoCyc
  • peptidoglycan-protein cross-linking Source: EcoCyc
  • regulation of cell shape Source: UniProtKB-KW

Keywordsi

Molecular functionGlycosyltransferase, Hydrolase, Transferase
Biological processCell shape, Cell wall biogenesis/degradation, Peptidoglycan synthesis

Enzyme and pathway databases

BioCyciEcoCyc:G6571-MONOMER
MetaCyc:G6571-MONOMER
UniPathwayiUPA00219

Protein family/group databases

MEROPSiC82.A05

Names & Taxonomyi

Protein namesi
Recommended name:
Probable L,D-transpeptidase YcfS (EC:2.-.-.-)
Gene namesi
Name:ycfS
Ordered Locus Names:b1113, JW5820
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi

Organism-specific databases

EcoGeneiEG13437 ycfS

Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

Periplasm

Pathology & Biotechi

Disruption phenotypei

Simultaneous disruption of erfK, ybiS, ycfS and ynhG leads to loss of covalent anchoring of the major outer membrane lipoprotein (Lpp) to the peptidoglycan. Complementation with ycfS restores some of this anchoring.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 23Sequence analysisAdd BLAST23
ChainiPRO_000001382624 – 320Probable L,D-transpeptidase YcfSAdd BLAST297

Proteomic databases

PaxDbiP75954
PRIDEiP75954

Interactioni

Subunit structurei

Interacts with DsbG.1 Publication

Protein-protein interaction databases

BioGridi4260085, 12 interactors
DIPiDIP-11546N
IntActiP75954, 2 interactors
STRINGi316385.ECDH10B_1185

Structurei

3D structure databases

ProteinModelPortaliP75954
SMRiP75954
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini45 – 90LysMPROSITE-ProRule annotationAdd BLAST46

Sequence similaritiesi

Belongs to the YkuD family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiENOG4107N1Q Bacteria
COG1376 LUCA
HOGENOMiHOG000274378
InParanoidiP75954
KOiK19236
OMAiIKDPTWT
PhylomeDBiP75954

Family and domain databases

CDDicd00118 LysM, 1 hit
Gene3Di2.40.440.10, 1 hit
InterProiView protein in InterPro
IPR005490 LD_TPept_cat_dom
IPR018392 LysM_dom
IPR038063 Transpep_catalytic_dom
PfamiView protein in Pfam
PF01476 LysM, 1 hit
PF03734 YkuD, 1 hit
SMARTiView protein in SMART
SM00257 LysM, 1 hit
SUPFAMiSSF141523 SSF141523, 2 hits
PROSITEiView protein in PROSITE
PS51782 LYSM, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P75954-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MMIKTRFSRW LTFFTFAAAV ALALPAKANT WPLPPAGSRL VGENKFHVVE
60 70 80 90 100
NDGGSLEAIA KKYNVGFLAL LQANPGVDPY VPRAGSVLTI PLQTLLPDAP
110 120 130 140 150
REGIVINIAE LRLYYYPPGK NSVTVYPIGI GQLGGDTLTP TMVTTVSDKR
160 170 180 190 200
ANPTWTPTAN IRARYKAQGI ELPAVVPAGL DNPMGHHAIR LAAYGGVYLL
210 220 230 240 250
HGTNADFGIG MRVSSGCIRL RDDDIKTLFS QVTPGTKVNI INTPIKVSAE
260 270 280 290 300
PNGARLVEVH QPLSEKIDDD PQLLPITLNS AMQSFKDAAQ TDAEVMQHVM
310 320
DVRSGMPVDV RRHQVSPQTL
Length:320
Mass (Da):34,636
Last modified:February 1, 1997 - v1
Checksum:i89378A8DFD60359B
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00096 Genomic DNA Translation: AAC74197.1
AP009048 Genomic DNA Translation: BAA35928.1
PIRiF64855
RefSeqiNP_415631.1, NC_000913.3

Genome annotation databases

EnsemblBacteriaiAAC74197; AAC74197; b1113
BAA35928; BAA35928; BAA35928
GeneIDi945666
KEGGiecj:JW5820
eco:b1113
PATRICifig|511145.12.peg.1157

Entry informationi

Entry nameiYCFS_ECOLI
AccessioniPrimary (citable) accession number: P75954
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: February 1, 1997
Last modified: March 28, 2018
This is version 129 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

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