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P75949

- NAGZ_ECOLI

UniProt

P75949 - NAGZ_ECOLI

Protein

Beta-hexosaminidase

Gene

nagZ

Organism
Escherichia coli (strain K12)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 116 (01 Oct 2014)
      Sequence version 1 (01 Feb 1997)
      Previous versions | rss
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    Functioni

    Plays a role in peptidoglycan recycling by cleaving the terminal beta-1,4-linked N-acetylglucosamine (GlcNAc) from peptide-linked peptidoglycan fragments, giving rise to free GlcNAc, anhydro-N-acetylmuramic acid and anhydro-N-acetylmuramic acid-linked peptides. Cleaves GlcNAc linked beta-1,4 to MurNAc tripeptides.

    Catalytic activityi

    Hydrolysis of terminal non-reducing N-acetyl-D-hexosamine residues in N-acetyl-beta-D-hexosaminides.UniRule annotation

    Enzyme regulationi

    Slightly inhibited by GlcNAc and N-acetylmuramic acid and strongly inhibited by N-acetylglucosaminolactone.

    pH dependencei

    Optimum pH is 7.7.

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei62 – 621SubstrateUniRule annotation
    Binding sitei70 – 701SubstrateUniRule annotation
    Binding sitei133 – 1331SubstrateUniRule annotation
    Sitei174 – 1741Important for catalytic activityUniRule annotation
    Active sitei176 – 1761Proton donor/acceptorUniRule annotation
    Active sitei248 – 2481NucleophileUniRule annotation

    GO - Molecular functioni

    1. beta-N-acetylhexosaminidase activity Source: UniProtKB-HAMAP

    GO - Biological processi

    1. carbohydrate metabolic process Source: InterPro
    2. cell cycle Source: UniProtKB-KW
    3. cell division Source: UniProtKB-KW
    4. peptidoglycan biosynthetic process Source: UniProtKB-KW
    5. peptidoglycan turnover Source: EcoliWiki
    6. regulation of cell shape Source: UniProtKB-KW

    Keywords - Molecular functioni

    Glycosidase, Hydrolase

    Keywords - Biological processi

    Cell cycle, Cell division, Cell shape, Cell wall biogenesis/degradation, Peptidoglycan synthesis

    Enzyme and pathway databases

    BioCyciEcoCyc:G6567-MONOMER.
    ECOL316407:JW1093-MONOMER.
    MetaCyc:G6567-MONOMER.
    UniPathwayiUPA00544.

    Protein family/group databases

    CAZyiGH3. Glycoside Hydrolase Family 3.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Beta-hexosaminidaseUniRule annotation (EC:3.2.1.52UniRule annotation)
    Alternative name(s):
    Beta-N-acetylhexosaminidaseUniRule annotation
    N-acetyl-beta-glucosaminidaseUniRule annotation
    Gene namesi
    Name:nagZUniRule annotation
    Synonyms:ycfO
    Ordered Locus Names:b1107, JW1093
    OrganismiEscherichia coli (strain K12)
    Taxonomic identifieri83333 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
    ProteomesiUP000000318: Chromosome, UP000000625: Chromosome

    Organism-specific databases

    EcoGeneiEG13433. nagZ.

    Subcellular locationi

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 341341Beta-hexosaminidasePRO_0000210785Add
    BLAST

    Proteomic databases

    PaxDbiP75949.
    PRIDEiP75949.

    Expressioni

    Gene expression databases

    GenevestigatoriP75949.

    Interactioni

    Subunit structurei

    Monomer.Curated

    Protein-protein interaction databases

    IntActiP75949. 8 interactions.
    STRINGi511145.b1107.

    Structurei

    3D structure databases

    ProteinModelPortaliP75949.
    SMRiP75949. Positions 1-340.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni163 – 1642Substrate bindingUniRule annotation

    Sequence similaritiesi

    Belongs to the glycosyl hydrolase 3 family. NagZ subfamily.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG1472.
    HOGENOMiHOG000248526.
    KOiK01207.
    OMAiAHDIDLS.
    OrthoDBiEOG6BCT06.
    PhylomeDBiP75949.

    Family and domain databases

    Gene3Di3.20.20.300. 1 hit.
    HAMAPiMF_00364. NagZ.
    InterProiIPR022956. Beta_hexosaminidase_bac.
    IPR019800. Glyco_hydro_3_AS.
    IPR001764. Glyco_hydro_3_N.
    IPR017853. Glycoside_hydrolase_SF.
    [Graphical view]
    PfamiPF00933. Glyco_hydro_3. 1 hit.
    [Graphical view]
    SUPFAMiSSF51445. SSF51445. 1 hit.
    PROSITEiPS00775. GLYCOSYL_HYDROL_F3. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P75949-1 [UniParc]FASTAAdd to Basket

    « Hide

    MGPVMLDVEG YELDAEEREI LAHPLVGGLI LFTRNYHDPA QLRELVRQIR    50
    AASRNRLVVA VDQEGGRVQR FREGFTRLPA AQSFAALSGM EEGGKLAQEA 100
    GWLMASEMIA MDIDISFAPV LDVGHISAAI GERSYHADPQ KALAIASRFI 150
    DGMHEAGMKT TGKHFPGHGA VTADSHKETP CDPRPQAEIR AKDMSVFSSL 200
    IRENKLDAIM PAHVIYSDVD PRPASGSPYW LKTVLRQELG FDGVIFSDDL 250
    SMEGAAIMGS YAERGQASLD AGCDMILVCN NRKGAVSVLD NLSPIKAERV 300
    TRLYHKGSFS RQELMDSARW KAISTRLNQL HERWQEEKAG H 341
    Length:341
    Mass (Da):37,595
    Last modified:February 1, 1997 - v1
    Checksum:i9E50C8E4DA87C7A4
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U00096 Genomic DNA. Translation: AAC74191.1.
    AP009048 Genomic DNA. Translation: BAA35914.1.
    PIRiH64854.
    RefSeqiNP_415625.1. NC_000913.3.
    YP_489375.1. NC_007779.1.

    Genome annotation databases

    EnsemblBacteriaiAAC74191; AAC74191; b1107.
    BAA35914; BAA35914; BAA35914.
    GeneIDi12930540.
    945671.
    KEGGiecj:Y75_p1077.
    eco:b1107.
    PATRICi32117457. VBIEscCol129921_1151.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U00096 Genomic DNA. Translation: AAC74191.1 .
    AP009048 Genomic DNA. Translation: BAA35914.1 .
    PIRi H64854.
    RefSeqi NP_415625.1. NC_000913.3.
    YP_489375.1. NC_007779.1.

    3D structure databases

    ProteinModelPortali P75949.
    SMRi P75949. Positions 1-340.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi P75949. 8 interactions.
    STRINGi 511145.b1107.

    Protein family/group databases

    CAZyi GH3. Glycoside Hydrolase Family 3.

    Proteomic databases

    PaxDbi P75949.
    PRIDEi P75949.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAC74191 ; AAC74191 ; b1107 .
    BAA35914 ; BAA35914 ; BAA35914 .
    GeneIDi 12930540.
    945671.
    KEGGi ecj:Y75_p1077.
    eco:b1107.
    PATRICi 32117457. VBIEscCol129921_1151.

    Organism-specific databases

    EchoBASEi EB3207.
    EcoGenei EG13433. nagZ.

    Phylogenomic databases

    eggNOGi COG1472.
    HOGENOMi HOG000248526.
    KOi K01207.
    OMAi AHDIDLS.
    OrthoDBi EOG6BCT06.
    PhylomeDBi P75949.

    Enzyme and pathway databases

    UniPathwayi UPA00544 .
    BioCyci EcoCyc:G6567-MONOMER.
    ECOL316407:JW1093-MONOMER.
    MetaCyc:G6567-MONOMER.

    Miscellaneous databases

    PROi P75949.

    Gene expression databases

    Genevestigatori P75949.

    Family and domain databases

    Gene3Di 3.20.20.300. 1 hit.
    HAMAPi MF_00364. NagZ.
    InterProi IPR022956. Beta_hexosaminidase_bac.
    IPR019800. Glyco_hydro_3_AS.
    IPR001764. Glyco_hydro_3_N.
    IPR017853. Glycoside_hydrolase_SF.
    [Graphical view ]
    Pfami PF00933. Glyco_hydro_3. 1 hit.
    [Graphical view ]
    SUPFAMi SSF51445. SSF51445. 1 hit.
    PROSITEi PS00775. GLYCOSYL_HYDROL_F3. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / MG1655 / ATCC 47076.
    3. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
      Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
      Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
    4. "Purification and properties of beta-N-acetylglucosaminidase from Escherichia coli."
      Yem D.W., Wu H.C.
      J. Bacteriol. 125:324-331(1976) [PubMed] [Europe PMC] [Abstract]
      Cited for: CHARACTERIZATION.
      Strain: K12.
    5. "Molecular characterization of the beta-N-acetylglucosaminidase of Escherichia coli and its role in cell wall recycling."
      Cheng Q., Li H., Merdek K., Park J.T.
      J. Bacteriol. 182:4836-4840(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: CHARACTERIZATION.
      Strain: K12.

    Entry informationi

    Entry nameiNAGZ_ECOLI
    AccessioniPrimary (citable) accession number: P75949
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 15, 1998
    Last sequence update: February 1, 1997
    Last modified: October 1, 2014
    This is version 116 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Escherichia coli
      Escherichia coli (strain K12): entries and cross-references to EcoGene
    2. Glycosyl hydrolases
      Classification of glycosyl hydrolase families and list of entries
    3. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    4. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3