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Protein

Thiamine kinase

Gene

thiK

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the phosphorylation of thiamine to thiamine phosphate.

Catalytic activityi

ATP + thiamine = ADP + thiamine phosphate.

Pathwayi: thiamine diphosphate biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes thiamine phosphate from thiamine.
Proteins known to be involved in this subpathway in this organism are:
  1. Thiamine kinase (thiK)
This subpathway is part of the pathway thiamine diphosphate biosynthesis, which is itself part of Cofactor biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes thiamine phosphate from thiamine, the pathway thiamine diphosphate biosynthesis and in Cofactor biosynthesis.

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • thiamine kinase activity Source: EcoCyc

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciEcoCyc:THIKIN-MONOMER.
ECOL316407:JW1092-MONOMER.
MetaCyc:THIKIN-MONOMER.
UniPathwayiUPA00060; UER00596.

Names & Taxonomyi

Protein namesi
Recommended name:
Thiamine kinase (EC:2.7.1.89)
Gene namesi
Name:thiK
Synonyms:ycfN
Ordered Locus Names:b1106, JW1092
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG13432. thiK.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 274274Thiamine kinasePRO_0000218057Add
BLAST

Proteomic databases

PaxDbiP75948.
PRIDEiP75948.

Interactioni

Protein-protein interaction databases

BioGridi4260073. 122 interactions.
IntActiP75948. 5 interactions.
STRINGi511145.b1106.

Structurei

3D structure databases

ProteinModelPortaliP75948.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the thiamine kinase family.Curated

Phylogenomic databases

eggNOGiENOG4108RUM. Bacteria.
COG0510. LUCA.
HOGENOMiHOG000219316.
KOiK07251.
OMAiDYLVLMW.

Family and domain databases

HAMAPiMF_01604. Thiamine_kinase. 1 hit.
InterProiIPR002575. Aminoglycoside_PTrfase.
IPR011009. Kinase-like_dom.
IPR014093. Thiamine_kinase.
[Graphical view]
PfamiPF01636. APH. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
TIGRFAMsiTIGR02721. ycfN_thiK. 1 hit.

Sequencei

Sequence statusi: Complete.

P75948-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPFRSNNPIT RDELLSRFFP QYHPVTTFNS GLSGGSFLIE HQGQRFVVRQ
60 70 80 90 100
PHDPDAPQSA FLRQYRALSQ LPACIAPKPH LYLRDWMVVD YLPGAVKTYL
110 120 130 140 150
PDTNELAGLL YYLHQQPRFG WRITLLPLLE LYWQQSDPAR RTVGWLRMLK
160 170 180 190 200
RLRKAREPRP LRLSPLHMDV HAGNLVHSAS GLKLIDWEYA GDGDIALELA
210 220 230 240 250
AVWVENTEQH RQLVNDYATR AKIYPAQLWR QVRRWFPWLL MLKAGWFEYR
260 270
WRQTGDQQFI RLADDTWRQL LIKQ
Length:274
Mass (Da):32,397
Last modified:February 1, 1997 - v1
Checksum:i0F4DBC46F43944CC
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00096 Genomic DNA. Translation: AAC74190.1.
AP009048 Genomic DNA. Translation: BAA35913.1.
PIRiG64854.
RefSeqiNP_415624.1. NC_000913.3.
WP_001116592.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC74190; AAC74190; b1106.
BAA35913; BAA35913; BAA35913.
GeneIDi948525.
KEGGiecj:JW1092.
eco:b1106.
PATRICi32117455. VBIEscCol129921_1150.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00096 Genomic DNA. Translation: AAC74190.1.
AP009048 Genomic DNA. Translation: BAA35913.1.
PIRiG64854.
RefSeqiNP_415624.1. NC_000913.3.
WP_001116592.1. NZ_LN832404.1.

3D structure databases

ProteinModelPortaliP75948.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4260073. 122 interactions.
IntActiP75948. 5 interactions.
STRINGi511145.b1106.

Proteomic databases

PaxDbiP75948.
PRIDEiP75948.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC74190; AAC74190; b1106.
BAA35913; BAA35913; BAA35913.
GeneIDi948525.
KEGGiecj:JW1092.
eco:b1106.
PATRICi32117455. VBIEscCol129921_1150.

Organism-specific databases

EchoBASEiEB3206.
EcoGeneiEG13432. thiK.

Phylogenomic databases

eggNOGiENOG4108RUM. Bacteria.
COG0510. LUCA.
HOGENOMiHOG000219316.
KOiK07251.
OMAiDYLVLMW.

Enzyme and pathway databases

UniPathwayiUPA00060; UER00596.
BioCyciEcoCyc:THIKIN-MONOMER.
ECOL316407:JW1092-MONOMER.
MetaCyc:THIKIN-MONOMER.

Miscellaneous databases

PROiP75948.

Family and domain databases

HAMAPiMF_01604. Thiamine_kinase. 1 hit.
InterProiIPR002575. Aminoglycoside_PTrfase.
IPR011009. Kinase-like_dom.
IPR014093. Thiamine_kinase.
[Graphical view]
PfamiPF01636. APH. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
TIGRFAMsiTIGR02721. ycfN_thiK. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiTHIK_ECOLI
AccessioniPrimary (citable) accession number: P75948
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: February 1, 1997
Last modified: September 7, 2016
This is version 105 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.