P75948 (THIK_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Thiamine kinase
EC=2.7.1.89

Gene names

Name:	thiK
Synonyms:	ycfN
Ordered Locus Names:	b1106, JW1092

Organism

Escherichia coli (strain K12) [Reference proteome] [HAMAP]

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia › Escherichia coli

Protein attributes

Sequence length	274 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Catalyzes the phosphorylation of thiamine to thiamine phosphate. HAMAP-Rule MF_01604
Catalytic activity	ATP + thiamine = ADP + thiamine phosphate. HAMAP-Rule MF_01604
Pathway	Cofactor biosynthesis; thiamine diphosphate biosynthesis; thiamine phosphate from thiamine: step 1/1. HAMAP-Rule MF_01604
Sequence similarities	Belongs to the thiamine kinase family.

Ontologies

Keywords
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	thiamine diphosphate biosynthetic process Inferred from electronic annotation. Source: UniProtKB-UniPathway thiamine metabolic process Inferred from electronic annotation. Source: InterPro
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW thiamine kinase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 274	274	Thiamine kinase HAMAP-Rule MF_01604		PRO_0000218057

Sequences

	Sequence		Length	Mass (Da)
	P75948 [UniParc]. Last modified February 1, 1997. Version 1. Checksum: 0F4DBC46F43944CC	FASTA	274	32,397
	10 20 30 40 50 60 MPFRSNNPIT RDELLSRFFP QYHPVTTFNS GLSGGSFLIE HQGQRFVVRQ PHDPDAPQSA 70 80 90 100 110 120 FLRQYRALSQ LPACIAPKPH LYLRDWMVVD YLPGAVKTYL PDTNELAGLL YYLHQQPRFG 130 140 150 160 170 180 WRITLLPLLE LYWQQSDPAR RTVGWLRMLK RLRKAREPRP LRLSPLHMDV HAGNLVHSAS 190 200 210 220 230 240 GLKLIDWEYA GDGDIALELA AVWVENTEQH RQLVNDYATR AKIYPAQLWR QVRRWFPWLL 250 260 270 MLKAGWFEYR WRQTGDQQFI RLADDTWRQL LIKQ

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"A 718-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 12.7-28.0 min region on the linkage map." Oshima T., Aiba H., Baba T., Fujita K., Hayashi K., Honjo A., Ikemoto K., Inada T., Itoh T., Kajihara M., Kanai K., Kashimoto K., Kimura S., Kitagawa M., Makino K., Masuda S., Miki T., Mizobuchi K. , Mori H., Motomura K., Nakamura Y., Nashimoto H., Nishio Y., Saito N., Sampei G., Seki Y., Tagami H., Takemoto K., Wada C., Yamamoto Y., Yano M., Horiuchi T. DNA Res. 3:137-155(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[2]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[3]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[4]	"Identification of the two missing bacterial genes involved in thiamine salvage: thiamine pyrophosphokinase and thiamine kinase." Melnick J., Lis E., Park J.-H., Kinsland C., Mori H., Baba T., Perkins J., Schyns G., Vassieva O., Osterman A., Begley T.P. J. Bacteriol. 186:3660-3662(2004) [PubMed] [Europe PMC] [Abstract] Cited for: CHARACTERIZATION.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U00096 Genomic DNA. Translation: AAC74190.1. AP009048 Genomic DNA. Translation: BAA35913.1.
PIR	G64854.
RefSeq	NP_415624.1. NC_000913.2. YP_489374.1. NC_007779.1.
3D structure databases
ProteinModelPortal	P75948.
ModBase	Search...
Protein-protein interaction databases
IntAct	P75948. 5 interactions.
STRING	511145.b1106.
Proteomic databases
PaxDb	P75948.
PRIDE	P75948.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAC74190; AAC74190; b1106. BAA35913; BAA35913; BAA35913.
GeneID	12931079. 948525.
KEGG	ecj:Y75_p1076. eco:b1106.
PATRIC	32117455. VBIEscCol129921_1150.
Organism-specific databases
EchoBASE	EB3206.
EcoGene	EG13432. thiK.
Phylogenomic databases
eggNOG	COG0510.
HOGENOM	HOG000219316.
KO	K07251.
OMA	DYLVLMW.
ProtClustDB	PRK10271.
Enzyme and pathway databases
BioCyc	EcoCyc:THIKIN-MONOMER. ECOL316407:JW1092-MONOMER. MetaCyc:THIKIN-MONOMER.
UniPathway	UPA00060; UER00596.
Gene expression databases
Genevestigator	P75948.
Family and domain databases
HAMAP	MF_01604. Thiamine_kinase.
InterPro	IPR002575. Aminoglycoside_PTrfase. IPR011009. Kinase-like_dom. IPR014093. Thiamine_kinase. [Graphical view]
Pfam	PF01636. APH. 1 hit. [Graphical view]
SUPFAM	SSF56112. Kinase_like. 1 hit.
TIGRFAMs	TIGR02721. ycfN_thiK. 1 hit.
ProtoNet	Search...

Entry information

Entry name

THIK_ECOLI

Accession

Primary (citable) accession number: P75948

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1998
Last sequence update:	February 1, 1997
Last modified:	May 1, 2013
This is version 88 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program