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P75942 (FLGJ_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Last modified March 19, 2014. Version 106. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Peptidoglycan hydrolase FlgJ

EC=3.2.1.-
Alternative name(s):
Muramidase FlgJ
Gene names
Name:flgJ
Synonyms:fla FX, flaZ
Ordered Locus Names:b1081, JW1068
OrganismEscherichia coli (strain K12) [Reference proteome] [HAMAP]
Taxonomic identifier83333 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia

Protein attributes

Sequence length313 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Flagellum-specific muramidase which hydrolyzes the peptidoglycan layer to assemble the rod structure in the periplasmic space By similarity.

Subcellular location

Periplasm By similarity.

Miscellaneous

Probably exported via the flagellum-specific export pathway.

Sequence similarities

In the N-terminal section; belongs to the FlgJ family.

In the C-terminal section; belongs to the glycosyl hydrolase 73 family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 313313Peptidoglycan hydrolase FlgJ
PRO_0000165705

Regions

Region148 – 313166Catalytic

Sites

Active site2201 Potential
Active site2451 Potential

Sequences

Sequence LengthMass (Da)Tools
P75942 [UniParc].

Last modified February 1, 1997. Version 1.
Checksum: C4B3CB8F36751CDF

FASTA31334,475
        10         20         30         40         50         60 
MISDSKLLAS AAWDAQSLNE LKAKAGEDPA ANIRPVARQV EGMFVQMMLK SMRDALPKDG 

        70         80         90        100        110        120 
LFSSEHTRLY TSMYDQQIAQ QMTAGKGLGL AEMMVKQMTP EQPLPEESTP AAPMKFPLET 

       130        140        150        160        170        180 
VVRYQNQALS QLVQKAVPRN YDDSLPGDSK AFLAQLSLPA QLASQQSGVP HHLILAQAAL 

       190        200        210        220        230        240 
ESGWGQRQIR RENGEPSYNL FGVKASGNWK GPVTEITTTE YENGEAKKVK AKFRVYSSYL 

       250        260        270        280        290        300 
EALSDYVGLL TRNPRYAAVT TAASAEQGAQ ALQDAGYATD PHYARKLTNM IQQMKSISDK 

       310 
VSKTYSMNID NLF 

« Hide

References

[1]"A 718-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 12.7-28.0 min region on the linkage map."
Oshima T., Aiba H., Baba T., Fujita K., Hayashi K., Honjo A., Ikemoto K., Inada T., Itoh T., Kajihara M., Kanai K., Kashimoto K., Kimura S., Kitagawa M., Makino K., Masuda S., Miki T., Mizobuchi K. expand/collapse author list , Mori H., Motomura K., Nakamura Y., Nashimoto H., Nishio Y., Saito N., Sampei G., Seki Y., Tagami H., Takemoto K., Wada C., Yamamoto Y., Yano M., Horiuchi T.
DNA Res. 3:137-155(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[2]"The complete genome sequence of Escherichia coli K-12."
Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y.
Science 277:1453-1462(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / MG1655 / ATCC 47076.
[3]"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / W3110 / ATCC 27325 / DSM 5911.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U00096 Genomic DNA. Translation: AAC74165.1.
AP009048 Genomic DNA. Translation: BAA35890.1.
PIRF64851.
RefSeqNP_415599.1. NC_000913.3.
YP_489349.1. NC_007779.1.

3D structure databases

ProteinModelPortalP75942.
SMRP75942. Positions 149-291.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

IntActP75942. 11 interactions.
STRING511145.b1081.

Protein family/group databases

CAZyGH73. Glycoside Hydrolase Family 73.

Proteomic databases

PRIDEP75942.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAC74165; AAC74165; b1081.
BAA35890; BAA35890; BAA35890.
GeneID12932026.
947456.
KEGGecj:Y75_p1051.
eco:b1081.
PATRIC32117403. VBIEscCol129921_1124.

Organism-specific databases

EchoBASEEB4020.
EcoGeneEG14272. flgJ.

Phylogenomic databases

eggNOGCOG3951.
HOGENOMHOG000265620.
KOK02395.
OMAPEVLIAQ.
OrthoDBEOG6KQ6M6.
ProtClustDBPRK05684.

Enzyme and pathway databases

BioCycEcoCyc:G366-MONOMER.
ECOL316407:JW1068-MONOMER.

Gene expression databases

GenevestigatorP75942.

Family and domain databases

InterProIPR019301. Flagellar_prot_FlgJ_N.
IPR013377. FlaJ.
IPR013338. Lysozyme_dom_subfam2.
IPR002901. Mano_Glyc_endo_b_GlcNAc.
[Graphical view]
PfamPF01832. Glucosaminidase. 1 hit.
PF10135. Rod-binding. 1 hit.
[Graphical view]
SMARTSM00047. LYZ2. 1 hit.
[Graphical view]
TIGRFAMsTIGR02541. flagell_FlgJ. 1 hit.
ProtoNetSearch...

Other

PROP75942.

Entry information

Entry nameFLGJ_ECOLI
AccessionPrimary (citable) accession number: P75942
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: February 1, 1997
Last modified: March 19, 2014
This is version 106 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Glycosyl hydrolases

Classification of glycosyl hydrolase families and list of entries

Escherichia coli

Escherichia coli (strain K12): entries and cross-references to EcoGene