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Protein

Peptidoglycan hydrolase FlgJ

Gene

flgJ

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Flagellum-specific muramidase which hydrolyzes the peptidoglycan layer to assemble the rod structure in the periplasmic space.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei220 – 2201Sequence Analysis
Active sitei245 – 2451Sequence Analysis

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Keywords - Biological processi

Bacterial flagellum biogenesis, Cell wall biogenesis/degradation

Enzyme and pathway databases

BioCyciEcoCyc:G366-MONOMER.
ECOL316407:JW1068-MONOMER.

Protein family/group databases

CAZyiGH73. Glycoside Hydrolase Family 73.

Names & Taxonomyi

Protein namesi
Recommended name:
Peptidoglycan hydrolase FlgJ (EC:3.2.1.-)
Alternative name(s):
Muramidase FlgJ
Gene namesi
Name:flgJ
Synonyms:fla FX, flaZ
Ordered Locus Names:b1081, JW1068
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
ProteomesiUP000000318 Componenti: Chromosome UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG14272. flgJ.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Periplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 313313Peptidoglycan hydrolase FlgJPRO_0000165705Add
BLAST

Proteomic databases

PRIDEiP75942.

Interactioni

Protein-protein interaction databases

IntActiP75942. 11 interactions.
STRINGi511145.b1081.

Structurei

3D structure databases

ProteinModelPortaliP75942.
SMRiP75942. Positions 149-291.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni148 – 313166CatalyticAdd
BLAST

Sequence similaritiesi

In the N-terminal section; belongs to the FlgJ family.Curated
In the C-terminal section; belongs to the glycosyl hydrolase 73 family.Curated

Phylogenomic databases

eggNOGiCOG3951.
HOGENOMiHOG000265620.
InParanoidiP75942.
KOiK02395.
OMAiEGVFVQM.
OrthoDBiEOG6KQ6M6.

Family and domain databases

InterProiIPR019301. Flagellar_prot_FlgJ_N.
IPR013377. FlaJ.
IPR013338. Lysozyme_subfam2_dom.
IPR002901. MGlyc_endo_b_GlcNAc_like_dom.
[Graphical view]
PfamiPF01832. Glucosaminidase. 1 hit.
PF10135. Rod-binding. 1 hit.
[Graphical view]
SMARTiSM00047. LYZ2. 1 hit.
[Graphical view]
TIGRFAMsiTIGR02541. flagell_FlgJ. 1 hit.

Sequencei

Sequence statusi: Complete.

P75942-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MISDSKLLAS AAWDAQSLNE LKAKAGEDPA ANIRPVARQV EGMFVQMMLK
60 70 80 90 100
SMRDALPKDG LFSSEHTRLY TSMYDQQIAQ QMTAGKGLGL AEMMVKQMTP
110 120 130 140 150
EQPLPEESTP AAPMKFPLET VVRYQNQALS QLVQKAVPRN YDDSLPGDSK
160 170 180 190 200
AFLAQLSLPA QLASQQSGVP HHLILAQAAL ESGWGQRQIR RENGEPSYNL
210 220 230 240 250
FGVKASGNWK GPVTEITTTE YENGEAKKVK AKFRVYSSYL EALSDYVGLL
260 270 280 290 300
TRNPRYAAVT TAASAEQGAQ ALQDAGYATD PHYARKLTNM IQQMKSISDK
310
VSKTYSMNID NLF
Length:313
Mass (Da):34,475
Last modified:February 1, 1997 - v1
Checksum:iC4B3CB8F36751CDF
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00096 Genomic DNA. Translation: AAC74165.1.
AP009048 Genomic DNA. Translation: BAA35890.1.
PIRiF64851.
RefSeqiNP_415599.1. NC_000913.3.

Genome annotation databases

EnsemblBacteriaiAAC74165; AAC74165; b1081.
BAA35890; BAA35890; BAA35890.
GeneIDi947456.
KEGGiecj:Y75_p1051.
eco:b1081.
PATRICi32117403. VBIEscCol129921_1124.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00096 Genomic DNA. Translation: AAC74165.1.
AP009048 Genomic DNA. Translation: BAA35890.1.
PIRiF64851.
RefSeqiNP_415599.1. NC_000913.3.

3D structure databases

ProteinModelPortaliP75942.
SMRiP75942. Positions 149-291.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP75942. 11 interactions.
STRINGi511145.b1081.

Protein family/group databases

CAZyiGH73. Glycoside Hydrolase Family 73.

Proteomic databases

PRIDEiP75942.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC74165; AAC74165; b1081.
BAA35890; BAA35890; BAA35890.
GeneIDi947456.
KEGGiecj:Y75_p1051.
eco:b1081.
PATRICi32117403. VBIEscCol129921_1124.

Organism-specific databases

EchoBASEiEB4020.
EcoGeneiEG14272. flgJ.

Phylogenomic databases

eggNOGiCOG3951.
HOGENOMiHOG000265620.
InParanoidiP75942.
KOiK02395.
OMAiEGVFVQM.
OrthoDBiEOG6KQ6M6.

Enzyme and pathway databases

BioCyciEcoCyc:G366-MONOMER.
ECOL316407:JW1068-MONOMER.

Miscellaneous databases

PROiP75942.

Family and domain databases

InterProiIPR019301. Flagellar_prot_FlgJ_N.
IPR013377. FlaJ.
IPR013338. Lysozyme_subfam2_dom.
IPR002901. MGlyc_endo_b_GlcNAc_like_dom.
[Graphical view]
PfamiPF01832. Glucosaminidase. 1 hit.
PF10135. Rod-binding. 1 hit.
[Graphical view]
SMARTiSM00047. LYZ2. 1 hit.
[Graphical view]
TIGRFAMsiTIGR02541. flagell_FlgJ. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  3. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
    Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
    Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.

Entry informationi

Entry nameiFLGJ_ECOLI
AccessioniPrimary (citable) accession number: P75942
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: February 1, 1997
Last modified: June 24, 2015
This is version 114 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Miscellaneous

Probably exported via the flagellum-specific export pathway.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.