Peptidoglycan hydrolase FlgJ - Escherichia coli (strain K12)

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P75942 (FLGJ_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 101. History...

Clusters with 100%, 90%, 50% identity |

Documents (3) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Peptidoglycan hydrolase FlgJ
EC=3.2.1.-

Alternative name(s):
Muramidase FlgJ

Gene names

Name:	flgJ
Synonyms:	fla FX, flaZ
Ordered Locus Names:	b1081, JW1068

Organism

Escherichia coli (strain K12) [Reference proteome] [HAMAP]

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia ›

Protein attributes

Sequence length	313 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Flagellum-specific muramidase which hydrolyzes the peptidoglycan layer to assemble the rod structure in the periplasmic space By similarity.
Subcellular location	Periplasm By similarity.
Miscellaneous	Probably exported via the flagellum-specific export pathway.
Sequence similarities	In the N-terminal section; belongs to the FlgJ family. In the C-terminal section; belongs to the glycosyl hydrolase 73 family.

Ontologies

Keywords
Biological process	Bacterial flagellum biogenesis Cell wall biogenesis/degradation
Cellular component	Periplasm
Molecular function	Glycosidase Hydrolase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	cell wall macromolecule metabolic process Inferred from electronic annotation. Source: InterPro ciliary or flagellar motility Inferred from electronic annotation. Source: InterPro flagellum assembly Inferred from electronic annotation. Source: InterPro peptidoglycan catabolic process Inferred from electronic annotation. Source: InterPro
Cellular_component	bacterial-type flagellum Inferred from electronic annotation. Source: InterPro periplasmic space Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	amidase activity Inferred from electronic annotation. Source: InterPro hydrolase activity, acting on glycosyl bonds Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 313

313

Peptidoglycan hydrolase FlgJ

PRO_0000165705

Regions

Region

148 – 313

166

Catalytic

Sites

Active site

220

Potential

Active site

245

Potential

Sequences

Sequence

Length

Mass (Da)

Tools

P75942 [UniParc].

Last modified February 1, 1997. Version 1.
Checksum: C4B3CB8F36751CDF
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FASTA

313

34,475

        10         20         30         40         50         60 
MISDSKLLAS AAWDAQSLNE LKAKAGEDPA ANIRPVARQV EGMFVQMMLK SMRDALPKDG 

        70         80         90        100        110        120 
LFSSEHTRLY TSMYDQQIAQ QMTAGKGLGL AEMMVKQMTP EQPLPEESTP AAPMKFPLET 

       130        140        150        160        170        180 
VVRYQNQALS QLVQKAVPRN YDDSLPGDSK AFLAQLSLPA QLASQQSGVP HHLILAQAAL 

       190        200        210        220        230        240 
ESGWGQRQIR RENGEPSYNL FGVKASGNWK GPVTEITTTE YENGEAKKVK AKFRVYSSYL 

       250        260        270        280        290        300 
EALSDYVGLL TRNPRYAAVT TAASAEQGAQ ALQDAGYATD PHYARKLTNM IQQMKSISDK 

       310 
VSKTYSMNID NLF

« Hide

References

[1]	"A 718-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 12.7-28.0 min region on the linkage map." Oshima T., Aiba H., Baba T., Fujita K., Hayashi K., Honjo A., Ikemoto K., Inada T., Itoh T., Kajihara M., Kanai K., Kashimoto K., Kimura S., Kitagawa M., Makino K., Masuda S., Miki T., Mizobuchi K. Horiuchi T. DNA Res. 3:137-155(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[2]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[3]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U00096 Genomic DNA. Translation: AAC74165.1. AP009048 Genomic DNA. Translation: BAA35890.1.
PIR	F64851.
RefSeq	NP_415599.1. NC_000913.2. YP_489349.1. NC_007779.1.
3D structure databases
ProteinModelPortal	P75942.
SMR	P75942. Positions 149-291.
ModBase	Search...
Protein-protein interaction databases
IntAct	P75942. 11 interactions.
STRING	511145.b1081.
Protein family/group databases
CAZy	GH73. Glycoside Hydrolase Family 73.
Proteomic databases
PRIDE	P75942.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAC74165; AAC74165; b1081. BAA35890; BAA35890; BAA35890.
GeneID	12932026. 947456.
KEGG	ecj:Y75_p1051. eco:b1081.
PATRIC	32117403. VBIEscCol129921_1124.
Organism-specific databases
EchoBASE	EB4020.
EcoGene	EG14272. flgJ.
Phylogenomic databases
eggNOG	COG3951.
HOGENOM	HOG000265620.
KO	K02395.
OMA	SMRDANA.
ProtClustDB	PRK05684.
Enzyme and pathway databases
BioCyc	EcoCyc:G366-MONOMER. ECOL316407:JW1068-MONOMER.
Gene expression databases
Genevestigator	P75942.
Family and domain databases
InterPro	IPR013377. FlaG_FlgJ-2. IPR000423. Flag_FlgJ_1. IPR019301. Flagellar_prot_FlgJ_N. IPR013338. Lysozyme_dom_subfam2. IPR002901. Mano_Glyc_endo_b_GlcNAc. [Graphical view]
Pfam	PF01832. Glucosaminidase. 1 hit. PF10135. Rod-binding. 1 hit. [Graphical view]
PRINTS	PR01002. FLGFLGJ.
SMART	SM00047. LYZ2. 1 hit. [Graphical view]
TIGRFAMs	TIGR02541. flagell_FlgJ. 1 hit.
ProtoNet	Search...

Entry information

Entry name

FLGJ_ECOLI

Accession

Primary (citable) accession number: P75942

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	February 1, 1997
Last modified:	May 1, 2013
This is version 101 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program

Relevant documents

Glycosyl hydrolases

Classification of glycosyl hydrolase families and list of entries

Escherichia coli

Escherichia coli (strain K12): entries and cross-references to EcoGene

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Sites

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Protein family/group databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Gene expression databases

Family and domain databases

Entry information

Relevant documents