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P75942

- FLGJ_ECOLI

UniProt

P75942 - FLGJ_ECOLI

Protein

Peptidoglycan hydrolase FlgJ

Gene

flgJ

Organism
Escherichia coli (strain K12)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 110 (01 Oct 2014)
      Sequence version 1 (01 Feb 1997)
      Previous versions | rss
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    Functioni

    Flagellum-specific muramidase which hydrolyzes the peptidoglycan layer to assemble the rod structure in the periplasmic space.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei220 – 2201Sequence Analysis
    Active sitei245 – 2451Sequence Analysis

    GO - Molecular functioni

    1. amidase activity Source: InterPro
    2. hydrolase activity, acting on glycosyl bonds Source: UniProtKB-KW

    GO - Biological processi

    1. bacterial-type flagellum assembly Source: InterPro
    2. bacterial-type flagellum-dependent cell motility Source: InterPro
    3. cell wall macromolecule metabolic process Source: InterPro
    4. peptidoglycan catabolic process Source: InterPro

    Keywords - Molecular functioni

    Glycosidase, Hydrolase

    Keywords - Biological processi

    Bacterial flagellum biogenesis, Cell wall biogenesis/degradation

    Enzyme and pathway databases

    BioCyciEcoCyc:G366-MONOMER.
    ECOL316407:JW1068-MONOMER.

    Protein family/group databases

    CAZyiGH73. Glycoside Hydrolase Family 73.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Peptidoglycan hydrolase FlgJ (EC:3.2.1.-)
    Alternative name(s):
    Muramidase FlgJ
    Gene namesi
    Name:flgJ
    Synonyms:fla FX, flaZ
    Ordered Locus Names:b1081, JW1068
    OrganismiEscherichia coli (strain K12)
    Taxonomic identifieri83333 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
    ProteomesiUP000000318: Chromosome, UP000000625: Chromosome

    Organism-specific databases

    EcoGeneiEG14272. flgJ.

    Subcellular locationi

    Periplasm By similarity

    GO - Cellular componenti

    1. bacterial-type flagellum Source: InterPro
    2. periplasmic space Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Periplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 313313Peptidoglycan hydrolase FlgJPRO_0000165705Add
    BLAST

    Proteomic databases

    PRIDEiP75942.

    Expressioni

    Gene expression databases

    GenevestigatoriP75942.

    Interactioni

    Protein-protein interaction databases

    IntActiP75942. 11 interactions.
    STRINGi511145.b1081.

    Structurei

    3D structure databases

    ProteinModelPortaliP75942.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni148 – 313166CatalyticAdd
    BLAST

    Sequence similaritiesi

    In the N-terminal section; belongs to the FlgJ family.Curated
    In the C-terminal section; belongs to the glycosyl hydrolase 73 family.Curated

    Phylogenomic databases

    eggNOGiCOG3951.
    HOGENOMiHOG000265620.
    KOiK02395.
    OMAiMLFKSMR.
    OrthoDBiEOG6KQ6M6.

    Family and domain databases

    InterProiIPR019301. Flagellar_prot_FlgJ_N.
    IPR013377. FlaJ.
    IPR013338. Lysozyme_subfam2_dom.
    IPR002901. MGlyc_endo_b_GlcNAc_like_dom.
    [Graphical view]
    PfamiPF01832. Glucosaminidase. 1 hit.
    PF10135. Rod-binding. 1 hit.
    [Graphical view]
    SMARTiSM00047. LYZ2. 1 hit.
    [Graphical view]
    TIGRFAMsiTIGR02541. flagell_FlgJ. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    P75942-1 [UniParc]FASTAAdd to Basket

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    MISDSKLLAS AAWDAQSLNE LKAKAGEDPA ANIRPVARQV EGMFVQMMLK    50
    SMRDALPKDG LFSSEHTRLY TSMYDQQIAQ QMTAGKGLGL AEMMVKQMTP 100
    EQPLPEESTP AAPMKFPLET VVRYQNQALS QLVQKAVPRN YDDSLPGDSK 150
    AFLAQLSLPA QLASQQSGVP HHLILAQAAL ESGWGQRQIR RENGEPSYNL 200
    FGVKASGNWK GPVTEITTTE YENGEAKKVK AKFRVYSSYL EALSDYVGLL 250
    TRNPRYAAVT TAASAEQGAQ ALQDAGYATD PHYARKLTNM IQQMKSISDK 300
    VSKTYSMNID NLF 313
    Length:313
    Mass (Da):34,475
    Last modified:February 1, 1997 - v1
    Checksum:iC4B3CB8F36751CDF
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U00096 Genomic DNA. Translation: AAC74165.1.
    AP009048 Genomic DNA. Translation: BAA35890.1.
    PIRiF64851.
    RefSeqiNP_415599.1. NC_000913.3.
    YP_489349.1. NC_007779.1.

    Genome annotation databases

    EnsemblBacteriaiAAC74165; AAC74165; b1081.
    BAA35890; BAA35890; BAA35890.
    GeneIDi12932026.
    947456.
    KEGGiecj:Y75_p1051.
    eco:b1081.
    PATRICi32117403. VBIEscCol129921_1124.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U00096 Genomic DNA. Translation: AAC74165.1 .
    AP009048 Genomic DNA. Translation: BAA35890.1 .
    PIRi F64851.
    RefSeqi NP_415599.1. NC_000913.3.
    YP_489349.1. NC_007779.1.

    3D structure databases

    ProteinModelPortali P75942.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi P75942. 11 interactions.
    STRINGi 511145.b1081.

    Protein family/group databases

    CAZyi GH73. Glycoside Hydrolase Family 73.

    Proteomic databases

    PRIDEi P75942.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAC74165 ; AAC74165 ; b1081 .
    BAA35890 ; BAA35890 ; BAA35890 .
    GeneIDi 12932026.
    947456.
    KEGGi ecj:Y75_p1051.
    eco:b1081.
    PATRICi 32117403. VBIEscCol129921_1124.

    Organism-specific databases

    EchoBASEi EB4020.
    EcoGenei EG14272. flgJ.

    Phylogenomic databases

    eggNOGi COG3951.
    HOGENOMi HOG000265620.
    KOi K02395.
    OMAi MLFKSMR.
    OrthoDBi EOG6KQ6M6.

    Enzyme and pathway databases

    BioCyci EcoCyc:G366-MONOMER.
    ECOL316407:JW1068-MONOMER.

    Miscellaneous databases

    PROi P75942.

    Gene expression databases

    Genevestigatori P75942.

    Family and domain databases

    InterProi IPR019301. Flagellar_prot_FlgJ_N.
    IPR013377. FlaJ.
    IPR013338. Lysozyme_subfam2_dom.
    IPR002901. MGlyc_endo_b_GlcNAc_like_dom.
    [Graphical view ]
    Pfami PF01832. Glucosaminidase. 1 hit.
    PF10135. Rod-binding. 1 hit.
    [Graphical view ]
    SMARTi SM00047. LYZ2. 1 hit.
    [Graphical view ]
    TIGRFAMsi TIGR02541. flagell_FlgJ. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / MG1655 / ATCC 47076.
    3. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
      Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
      Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / W3110 / ATCC 27325 / DSM 5911.

    Entry informationi

    Entry nameiFLGJ_ECOLI
    AccessioniPrimary (citable) accession number: P75942
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1997
    Last sequence update: February 1, 1997
    Last modified: October 1, 2014
    This is version 110 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Probably exported via the flagellum-specific export pathway.

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Escherichia coli
      Escherichia coli (strain K12): entries and cross-references to EcoGene
    2. Glycosyl hydrolases
      Classification of glycosyl hydrolase families and list of entries
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3