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P75914 (YCDX_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 90. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Probable phosphatase YcdX
EC=3.1.3.-
Gene names
Name:ycdX
Ordered Locus Names:b1034, JW1017
OrganismEscherichia coli (strain K12)
Taxonomic identifier83333 [NCBI]
Taxonomic lineageBacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length245 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Hydrolyzes p-nitrophenyl phosphate (pNPP) in vitro. Involved in the swarming motility process. Ref.4

Cofactor

Binds 3 zinc ions per subunit.

Enzyme regulation

Activity is stimulated by YcdY. Ref.4

Subunit structure

Homotrimer. Interacts with YcdY. Ref.4 Ref.5

Disruption phenotype

Mutants show no swarming ability. Ref.4

Sequence similarities

Belongs to the PHP family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 245245Probable phosphatase YcdX HAMAP MF_01561
PRO_0000013818

Sites

Metal binding71Zinc 1
Metal binding91Zinc 1
Metal binding151Zinc 2
Metal binding401Zinc 2
Metal binding731Zinc 1
Metal binding731Zinc 3
Metal binding1011Zinc 3
Metal binding1311Zinc 3
Metal binding1921Zinc 1
Metal binding1941Zinc 2

Secondary structure

1 ........................................... 245
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P75914 [UniParc].

Last modified February 1, 1997. Version 1.
Checksum: 19ADC0EF462D49D3

FASTA24526,891
        10         20         30         40         50         60 
MYPVDLHMHT VASTHAYSTL SDYIAQAKQK GIKLFAITDH GPDMEDAPHH WHFINMRIWP 

        70         80         90        100        110        120 
RVVDGVGILR GIEANIKNVD GEIDCSGKMF DSLDLIIAGF HEPVFAPHDK ATNTQAMIAT 

       130        140        150        160        170        180 
IASGNVHIIS HPGNPKYEID VKAVAEAAAK HQVALEINNS SFLHSRKGSE DNCREVAAAV 

       190        200        210        220        230        240 
RDAGGWVALG SDSHTAFTMG EFEECLKILD AVDFPPERIL NVSPRRLLNF LESRGMAPIA 


EFADL

« Hide

References

« Hide 'large scale' references
[1]"A 718-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 12.7-28.0 min region on the linkage map."
Oshima T., Aiba H., Baba T., Fujita K., Hayashi K., Honjo A., Ikemoto K., Inada T., Itoh T., Kajihara M., Kanai K., Kashimoto K., Kimura S., Kitagawa M., Makino K., Masuda S., Miki T., Mizobuchi K. expand/collapse author list , Mori H., Motomura K., Nakamura Y., Nashimoto H., Nishio Y., Saito N., Sampei G., Seki Y., Tagami H., Takemoto K., Wada C., Yamamoto Y., Yano M., Horiuchi T.
DNA Res. 3:137-155(1996) [PubMed: 8905232] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[2]"The complete genome sequence of Escherichia coli K-12."
Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y.
Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / MG1655 / ATCC 47076.
[3]"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
Mol. Syst. Biol. 2:E1-E5(2006) [PubMed: 16738553] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[4]"YcdY protein of Escherichia coli, an atypical member of the TorD chaperone family."
Redelberger D., Seduk F., Genest O., Mejean V., Leimkuhler S., Iobbi-Nivol C.
J. Bacteriol. 193:6512-6516(2011) [PubMed: 21965574] [Abstract]
Cited for: FUNCTION, ENZYME REGULATION, INTERACTION WITH YCDY, DISRUPTION PHENOTYPE, IDENTIFICATION BY MASS SPECTROMETRY.
Strain: K12 / BW25113.
[5]"Crystal structure of the Escherichia coli YcdX protein reveals a trinuclear zinc active site."
Teplyakov A., Obmolova G., Khil P.P., Howard A.J., Camerini-Otero R.D., Gilliland G.L.
Proteins 51:315-318(2003) [PubMed: 12661000] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (1.57 ANGSTROMS) IN COMPLEX WITH ZINC IONS, SUBUNIT.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		U00096 Genomic DNA. Translation: AAC74118.1.
AP009048 Genomic DNA. Translation: BAA35815.1.
PIRG64845.
RefSeqNP_415552.1. NC_000913.2.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1M65X-ray1.57A1-245[»]
1M68X-ray2.30A1-245[»]
1PB0X-ray1.95A/B/C1-245[»]
ProteinModelPortalP75914.
SMRP75914. Positions 2-245.
ModBaseSearch...

Protein-protein interaction databases

IntActP75914. 7 interactions.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaEBESCT00000001132; EBESCP00000001132; EBESCG00000000935.
EBESCT00000014505; EBESCP00000013796; EBESCG00000013566.
GeneID948477.
GenomeReviewsGene locus JW1017 in contig AP009048_GR.
Gene locus b1034 in contig U00096_GR.
KEGGecj:JW1017.
eco:b1034.
PATRIC32117299. VBIEscCol129921_1072.

Organism-specific databases

EchoBASEEB3629.
EcoGeneEG13870. ycdX.

Phylogenomic databases

eggNOGCOG1387.
GeneTreeEBGT00050000010657.
HOGENOMHBG734021.
OMAHTIASTH.
PhylomeDBP75914.
ProtClustDBPRK09248.

Enzyme and pathway databases

BioCycEcoCyc:G6540-MONOMER.

Gene expression databases

GenevestigatorP75914.

Family and domain databases

HAMAPMF_01561. YcdX_phosphat.
[Tree]
InterProIPR023710. Hydrolase_YcdX_put.
IPR004013. PHP_C.
IPR003141. Pol/His_phosphatase_N.
IPR016195. Pol/histidinol_Pase-like.
[Graphical view]
KOK04477.
PfamPF02811. PHP. 1 hit.
[Graphical view]
SMARTSM00481. POLIIIAc. 1 hit.
[Graphical view]
SUPFAMSSF89550. PHP-like. 1 hit.
ProtoNetSearch...

Entry information

Entry nameYCDX_ECOLI
AccessionPrimary (citable) accession number: P75914
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: February 1, 1997
Last modified: January 25, 2012
This is version 90 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

Escherichia coli

Escherichia coli (strain K12): entries and cross-references to EcoGene

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

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