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Protein

Glyoxylate/hydroxypyruvate reductase A

Gene

ghrA

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes the NADPH-dependent reduction of glyoxylate and hydroxypyruvate into glycolate and glycerate, respectively. Inactive towards 2-oxo-D-gluconate, 2-oxoglutarate, oxaloacetate and pyruvate. Only D- and L-glycerate are involved in the oxidative activity with NADP. Activity with NAD is very low.

Catalytic activityi

Glycolate + NADP+ = glyoxylate + NADPH.1 Publication
D-glycerate + NAD(P)+ = hydroxypyruvate + NAD(P)H.1 Publication

Kineticsi

The catalytic efficiency is better for glyoxylate than hydroxypyruvate with NADPH as electron donor.
  1. KM=0.6 mM for glyoxylate (at 25 degrees Celsius and pH 7)1 Publication
  2. KM=1.0 mM for hydroxypyruvate (at 25 degrees Celsius and pH 7)1 Publication
  1. Vmax=120 µmol/min/mg enzyme with glyoxylate as substrate (at 25 degrees Celsius and pH 7)1 Publication
  2. Vmax=20 µmol/min/mg enzyme with hydroxypyruvate as substrate (at 25 degrees Celsius and pH 7)1 Publication

pH dependencei

Optimum pH is 7.0.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei227By similarity1
Active sitei275Proton donorBy similarity1

GO - Molecular functioni

  • glyoxylate reductase (NADP) activity Source: EcoCyc
  • hydroxypyruvate reductase activity Source: EcoCyc
  • NAD binding Source: InterPro

Keywordsi

Molecular functionOxidoreductase
LigandNAD, NADP

Enzyme and pathway databases

BioCyciEcoCyc:G6539-MONOMER
MetaCyc:G6539-MONOMER
SABIO-RKP75913

Names & Taxonomyi

Protein namesi
Recommended name:
Glyoxylate/hydroxypyruvate reductase A (EC:1.1.1.79, EC:1.1.1.81)
Alternative name(s):
2-ketoacid reductase
Gene namesi
Name:ghrA
Synonyms:ycdW
Ordered Locus Names:b1033, JW5146
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG13869 ghrA

Subcellular locationi

  • Cytoplasm 1 Publication

GO - Cellular componenti

  • cytosol Source: EcoCyc

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000760281 – 312Glyoxylate/hydroxypyruvate reductase AAdd BLAST312

Proteomic databases

EPDiP75913
PaxDbiP75913
PRIDEiP75913

Expressioni

Inductioni

Constitutively expressed.1 Publication

Interactioni

Protein-protein interaction databases

BioGridi4260060, 17 interactors
IntActiP75913, 1 interactor
STRINGi316385.ECDH10B_1105

Structurei

3D structure databases

ProteinModelPortaliP75913
SMRiP75913
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiENOG4105JPG Bacteria
COG0111 LUCA
HOGENOMiHOG000136694
InParanoidiP75913
KOiK12972
OMAiVQMAEYV
PhylomeDBiP75913

Family and domain databases

HAMAPiMF_01666 2_Hacid_dh_C_GhrA, 1 hit
InterProiView protein in InterPro
IPR029753 D-isomer_DH_CS
IPR006140 D-isomer_DH_NAD-bd
IPR023514 GhrA
IPR036291 NAD(P)-bd_dom_sf
PfamiView protein in Pfam
PF02826 2-Hacid_dh_C, 1 hit
SUPFAMiSSF51735 SSF51735, 1 hit
PROSITEiView protein in PROSITE
PS00671 D_2_HYDROXYACID_DH_3, 1 hit

Sequencei

Sequence statusi: Complete.

P75913-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDIIFYHPTF DTQWWIEALR KAIPQARVRA WKSGDNDSAD YALVWHPPVE
60 70 80 90 100
MLAGRDLKAV FALGAGVDSI LSKLQAHPEM LNPSVPLFRL EDTGMGEQMQ
110 120 130 140 150
EYAVSQVLHW FRRFDDYRIQ QNSSHWQPLP EYHREDFTIG ILGAGVLGSK
160 170 180 190 200
VAQSLQTWRF PLRCWSRTRK SWPGVQSFAG REELSAFLSQ CRVLINLLPN
210 220 230 240 250
TPETVGIINQ QLLEKLPDGA YLLNLARGVH VVEDDLLAAL DSGKVKGAML
260 270 280 290 300
DVFNREPLPP ESPLWQHPRV TITPHVAAIT RPAEAVEYIS RTIAQLEKGE
310
RVCGQVDRAR GY
Length:312
Mass (Da):35,343
Last modified:November 13, 2007 - v2
Checksum:i5B2F966D11DC6B40
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00096 Genomic DNA Translation: AAC74117.2
AP009048 Genomic DNA Translation: BAA35814.1
PIRiF64845
RefSeqiNP_415551.2, NC_000913.3
WP_000351317.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaiAAC74117; AAC74117; b1033
BAA35814; BAA35814; BAA35814
GeneIDi946431
KEGGiecj:JW5146
eco:b1033
PATRICifig|1411691.4.peg.1238

Similar proteinsi

Entry informationi

Entry nameiGHRA_ECOLI
AccessioniPrimary (citable) accession number: P75913
Secondary accession number(s): Q9R3M8
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 13, 2007
Last modified: March 28, 2018
This is version 131 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

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