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Protein

Glyoxylate/hydroxypyruvate reductase A

Gene

ghrA

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the NADPH-dependent reduction of glyoxylate and hydroxypyruvate into glycolate and glycerate, respectively. Inactive towards 2-oxo-D-gluconate, 2-oxoglutarate, oxaloacetate and pyruvate. Only D- and L-glycerate are involved in the oxidative activity with NADP. Activity with NAD is very low.

Catalytic activityi

Glycolate + NADP+ = glyoxylate + NADPH.1 Publication
D-glycerate + NAD(P)+ = hydroxypyruvate + NAD(P)H.1 Publication

Kineticsi

The catalytic efficiency is better for glyoxylate than hydroxypyruvate with NADPH as electron donor.

  1. KM=0.6 mM for glyoxylate (at 25 degrees Celsius and pH 7)1 Publication
  2. KM=1.0 mM for hydroxypyruvate (at 25 degrees Celsius and pH 7)1 Publication
  1. Vmax=120 µmol/min/mg enzyme with glyoxylate as substrate (at 25 degrees Celsius and pH 7)1 Publication
  2. Vmax=20 µmol/min/mg enzyme with hydroxypyruvate as substrate (at 25 degrees Celsius and pH 7)1 Publication

pH dependencei

Optimum pH is 7.0.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei227By similarity1
Active sitei275Proton donorBy similarity1

GO - Molecular functioni

  • glyoxylate reductase (NADP) activity Source: EcoCyc
  • hydroxypyruvate reductase activity Source: EcoCyc
  • NAD binding Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Ligandi

NAD, NADP

Enzyme and pathway databases

BioCyciEcoCyc:G6539-MONOMER.
ECOL316407:JW5146-MONOMER.
MetaCyc:G6539-MONOMER.
SABIO-RKP75913.

Names & Taxonomyi

Protein namesi
Recommended name:
Glyoxylate/hydroxypyruvate reductase A (EC:1.1.1.79, EC:1.1.1.81)
Alternative name(s):
2-ketoacid reductase
Gene namesi
Name:ghrA
Synonyms:ycdW
Ordered Locus Names:b1033, JW5146
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG13869. ghrA.

Subcellular locationi

  • Cytoplasm 1 Publication

GO - Cellular componenti

  • cytosol Source: EcoCyc
  • plasma membrane Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000760281 – 312Glyoxylate/hydroxypyruvate reductase AAdd BLAST312

Proteomic databases

EPDiP75913.
PaxDbiP75913.
PRIDEiP75913.

Expressioni

Inductioni

Constitutively expressed.1 Publication

Interactioni

Protein-protein interaction databases

BioGridi4260060. 17 interactors.
IntActiP75913. 1 interactor.
STRINGi511145.b1033.

Structurei

3D structure databases

ProteinModelPortaliP75913.
SMRiP75913.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiENOG4105JPG. Bacteria.
COG0111. LUCA.
HOGENOMiHOG000136694.
InParanoidiP75913.
KOiK12972.
OMAiLLISSHC.
PhylomeDBiP75913.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
HAMAPiMF_01666. 2_Hacid_dh_C_GhrA. 1 hit.
InterProiIPR029753. D-isomer_DH_CS.
IPR006140. D-isomer_DH_NAD-bd.
IPR023514. GhrA.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PfamiPF02826. 2-Hacid_dh_C. 1 hit.
[Graphical view]
SUPFAMiSSF51735. SSF51735. 1 hit.
PROSITEiPS00671. D_2_HYDROXYACID_DH_3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P75913-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDIIFYHPTF DTQWWIEALR KAIPQARVRA WKSGDNDSAD YALVWHPPVE
60 70 80 90 100
MLAGRDLKAV FALGAGVDSI LSKLQAHPEM LNPSVPLFRL EDTGMGEQMQ
110 120 130 140 150
EYAVSQVLHW FRRFDDYRIQ QNSSHWQPLP EYHREDFTIG ILGAGVLGSK
160 170 180 190 200
VAQSLQTWRF PLRCWSRTRK SWPGVQSFAG REELSAFLSQ CRVLINLLPN
210 220 230 240 250
TPETVGIINQ QLLEKLPDGA YLLNLARGVH VVEDDLLAAL DSGKVKGAML
260 270 280 290 300
DVFNREPLPP ESPLWQHPRV TITPHVAAIT RPAEAVEYIS RTIAQLEKGE
310
RVCGQVDRAR GY
Length:312
Mass (Da):35,343
Last modified:November 13, 2007 - v2
Checksum:i5B2F966D11DC6B40
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00096 Genomic DNA. Translation: AAC74117.2.
AP009048 Genomic DNA. Translation: BAA35814.1.
PIRiF64845.
RefSeqiNP_415551.2. NC_000913.3.
WP_000351317.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC74117; AAC74117; b1033.
BAA35814; BAA35814; BAA35814.
GeneIDi946431.
KEGGiecj:JW5146.
eco:b1033.
PATRICi32117297. VBIEscCol129921_1071.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00096 Genomic DNA. Translation: AAC74117.2.
AP009048 Genomic DNA. Translation: BAA35814.1.
PIRiF64845.
RefSeqiNP_415551.2. NC_000913.3.
WP_000351317.1. NZ_LN832404.1.

3D structure databases

ProteinModelPortaliP75913.
SMRiP75913.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4260060. 17 interactors.
IntActiP75913. 1 interactor.
STRINGi511145.b1033.

Proteomic databases

EPDiP75913.
PaxDbiP75913.
PRIDEiP75913.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC74117; AAC74117; b1033.
BAA35814; BAA35814; BAA35814.
GeneIDi946431.
KEGGiecj:JW5146.
eco:b1033.
PATRICi32117297. VBIEscCol129921_1071.

Organism-specific databases

EchoBASEiEB3628.
EcoGeneiEG13869. ghrA.

Phylogenomic databases

eggNOGiENOG4105JPG. Bacteria.
COG0111. LUCA.
HOGENOMiHOG000136694.
InParanoidiP75913.
KOiK12972.
OMAiLLISSHC.
PhylomeDBiP75913.

Enzyme and pathway databases

BioCyciEcoCyc:G6539-MONOMER.
ECOL316407:JW5146-MONOMER.
MetaCyc:G6539-MONOMER.
SABIO-RKP75913.

Miscellaneous databases

PROiP75913.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
HAMAPiMF_01666. 2_Hacid_dh_C_GhrA. 1 hit.
InterProiIPR029753. D-isomer_DH_CS.
IPR006140. D-isomer_DH_NAD-bd.
IPR023514. GhrA.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PfamiPF02826. 2-Hacid_dh_C. 1 hit.
[Graphical view]
SUPFAMiSSF51735. SSF51735. 1 hit.
PROSITEiPS00671. D_2_HYDROXYACID_DH_3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiGHRA_ECOLI
AccessioniPrimary (citable) accession number: P75913
Secondary accession number(s): Q9R3M8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 13, 2007
Last modified: November 2, 2016
This is version 122 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.