P75913 (GHRA_ECOLI) Reviewed, UniProtKB/Swiss-Prot
Last modified
January 25, 2012.
Version 87.
History...
Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order
Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize orderNames and origin
| Protein names | Recommended name: Glyoxylate/hydroxypyruvate reductase A EC=1.1.1.79 EC=1.1.1.81 Alternative name(s): 2-ketoacid reductase | ||||||
| Gene names |
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| Organism | Escherichia coli (strain K12) | ||||||
| Taxonomic identifier | 83333 [NCBI] | ||||||
| Taxonomic lineage | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia |
Protein attributes
| Sequence length | 312 AA. |
| Sequence status | Complete. |
| Protein existence | Evidence at protein level |
General annotation (Comments)
| Function | Catalyzes the NADPH-dependent reduction of glyoxylate and hydroxypyruvate into glycolate and glycerate, respectively. Inactive towards 2-oxo-D-gluconate, 2-oxoglutarate, oxaloacetate and pyruvate. Only D- and L-glycerate are involved in the oxidative activity with NADP. Activity with NAD is very low. HAMAP MF_01666 |
| Catalytic activity | Glycolate + NADP+ = glyoxylate + NADPH. Ref.4 D-glycerate + NAD(P)+ = hydroxypyruvate + NAD(P)H. Ref.4 |
| Subcellular location | |
| Induction | Constitutively expressed. Ref.4 |
| Sequence similarities | Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family. GhrA subfamily. |
| Biophysicochemical properties | Kinetic parameters: The catalytic efficiency is better for glyoxylate than hydroxypyruvate with NADPH as electron donor. KM=0.6 mM for glyoxylate (at 25 degrees Celsius and pH 7) Ref.4 KM=1.0 mM for hydroxypyruvate (at 25 degrees Celsius and pH 7) Vmax=120 µmol/min/mg enzyme with glyoxylate as substrate (at 25 degrees Celsius and pH 7) Vmax=20 µmol/min/mg enzyme with hydroxypyruvate as substrate (at 25 degrees Celsius and pH 7) pH dependence: Optimum pH is 7.0. |
Ontologies
| Keywords | |
|---|---|
| Cellular component | Cytoplasm |
| Ligand | NAD NADP |
| Molecular function | Oxidoreductase |
| Technical term | Complete proteome Direct protein sequencing Reference proteome |
| Gene Ontology (GO) | |
| Biological process | cellular response to stress Inferred from expression pattern. Source: EcoCyc |
| Cellular component | cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell |
| Molecular function | cofactor binding Inferred from electronic annotation. Source: InterPro glyoxylate reductase (NADP) activityInferred from direct assay Ref.4. Source: EcoCyc hydroxypyruvate reductase activityInferred from direct assay Ref.4. Source: EcoCyc nucleotide bindingInferred from electronic annotation. Source: InterPro |
| Complete GO annotation... | |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Chain | 1 – 312 | 312 | Glyoxylate/hydroxypyruvate reductase A HAMAP MF_01666 | PRO_0000076028 | |||||
Sites | |||||||||
| Active site | 227 | 1 | By similarity | ||||||
| Active site | 275 | 1 | Proton donor By similarity | ||||||
Sequences
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References
| « Hide 'large scale' references | |
| [1] | "A 718-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 12.7-28.0 min region on the linkage map." Oshima T., Aiba H., Baba T., Fujita K., Hayashi K., Honjo A., Ikemoto K., Inada T., Itoh T., Kajihara M., Kanai K., Kashimoto K., Kimura S., Kitagawa M., Makino K., Masuda S., Miki T., Mizobuchi K. Horiuchi T.DNA Res. 3:137-155(1996) [PubMed: 8905232] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911. |
| [2] | "The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076. |
| [3] | "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed: 16738553] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911. |
| [4] | "Biochemical characterization of the 2-ketoacid reductases encoded by ycdW and yiaE genes in Escherichia coli." Nunez M.F., Pellicer M.T., Badia J., Aguilar J., Baldoma L. Biochem. J. 354:707-715(2001) [PubMed: 11237876] [Abstract] Cited for: PROTEIN SEQUENCE OF 1-10, CATALYTIC ACTIVITY, SUBSTRATE SPECIFICITY, INDUCTION, SUBCELLULAR LOCATION, BIOPHYSICOCHEMICAL PROPERTIES. Strain: K12 / MC4100 / ATCC 35695 / DSM 6574. |
| [5] | "Enrichment of low abundance proteins of Escherichia coli by hydroxyapatite chromatography." Fountoulakis M., Takacs M.-F., Berndt P., Langen H., Takacs B. Electrophoresis 20:2181-2195(1999) [PubMed: 10493123] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY. Strain: B / BL21. |
Cross-references
Sequence databases | |
|---|---|
| EMBL GenBank DDBJ | U00096 Genomic DNA. Translation: AAC74117.2. AP009048 Genomic DNA. Translation: BAA35814.1. |
| PIR | F64845. |
| RefSeq | NP_415551.2. NC_000913.2. |
3D structure databases | |
| ProteinModelPortal | P75913. |
| SMR | P75913. Positions 1-312. |
| ModBase | Search... |
Protocols and materials databases | |
| StructuralBiologyKnowledgebase | Search... |
Genome annotation databases | |
| EnsemblBacteria | EBESCT00000002891; EBESCP00000002891; EBESCG00000002359. EBESCT00000016045; EBESCP00000015336; EBESCG00000015105. |
| GeneID | 946431. |
| GenomeReviews | Gene locus JW5146 in contig AP009048_GR. Gene locus b1033 in contig U00096_GR. |
| KEGG | ecj:JW5146. eco:b1033. |
| PATRIC | 32117297. VBIEscCol129921_1071. |
Organism-specific databases | |
| EchoBASE | EB3628. |
| EcoGene | EG13869. ghrA. |
Phylogenomic databases | |
| eggNOG | COG0111. |
| GeneTree | EBGT00050000009080. |
| HOGENOM | HBG731446. |
| OMA | EYVTHAV. |
| PhylomeDB | P75913. |
| ProtClustDB | PRK15469. |
Enzyme and pathway databases | |
| BioCyc | EcoCyc:G6539-MONOMER. MetaCyc:G6539-MONOMER. |
Gene expression databases | |
| Genevestigator | P75913. |
Family and domain databases | |
| HAMAP | MF_01666. 2-Hacid_dh_C_GhrA. [Tree] |
| InterPro | IPR006140. D-isomer_2_OHA_DH_NAD-bd. IPR023514. GhrA. IPR016040. NAD(P)-bd_dom. [Graphical view] |
| Gene3D | G3DSA:3.40.50.720. NAD(P)-bd. 1 hit. |
| KO | K12972. |
| Pfam | PF02826. 2-Hacid_dh_C. 1 hit. [Graphical view] |
| PROSITE | PS00065. D_2_HYDROXYACID_DH_1. False negative. PS00670. D_2_HYDROXYACID_DH_2. False negative. PS00671. D_2_HYDROXYACID_DH_3. 1 hit. [Graphical view] |
| ProtoNet | Search... |
Entry information
| Entry name | GHRA_ECOLI | ||||||||
| Accession | Primary (citable) accession number: P75913 Secondary accession number(s): Q9R3M8 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation program | Prokaryotic Protein Annotation Program | ||||||||
Relevant documents
| Escherichia coli Escherichia coli (strain K12): entries and cross-references to EcoGene |
| SIMILARITY comments Index of protein domains and families |

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