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Protein

Probable diguanylate cyclase DgcT

Gene

dgcT

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Probably catalyzes the synthesis of cyclic-di-GMP (c-di-GMP) via the condensation of 2 GTP molecules. Overexpression leads to a strong repression of swimming; swimming returns to normal when residues 359-360 are both mutated to Ala. Overexpression also leads to a 20-fold increase in c-di-GMP levels in vivo. Cyclic-di-GMP is a second messenger which controls cell surface-associated traits in bacteria.1 Publication

Catalytic activityi

2 GTP = 2 diphosphate + cyclic di-3',5'-guanylate.1 Publication

Cofactori

Mg2+By similarityNote: Binds 1 Mg2+ ion per monomer.By similarity

Pathwayi: 3',5'-cyclic di-GMP biosynthesis

This protein is involved in the pathway 3',5'-cyclic di-GMP biosynthesis, which is part of Purine metabolism.Curated
View all proteins of this organism that are known to be involved in the pathway 3',5'-cyclic di-GMP biosynthesis and in Purine metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi318MagnesiumBy similarity1
Metal bindingi319Magnesium; via carbonyl oxygenBy similarity1
Sitei323Transition state stabilizerSequence analysis1
Binding sitei326SubstrateBy similarity1
Binding sitei331Substrate; via carbonyl oxygenBy similarity1
Binding sitei335SubstrateBy similarity1
Active sitei361Proton acceptorSequence analysis1
Metal bindingi361MagnesiumBy similarity1
Binding sitei381SubstrateBy similarity1

GO - Molecular functioni

  • diguanylate cyclase activity Source: EcoCyc
  • GTP binding Source: UniProtKB-KW
  • metal ion binding Source: UniProtKB-KW

GO - Biological processi

  • negative regulation of cellular component movement Source: EcoCyc
  • single-species submerged biofilm formation Source: EcoCyc

Keywordsi

Molecular functionTransferase
LigandGTP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciEcoCyc:G6532-MONOMER
MetaCyc:G6532-MONOMER
UniPathwayiUPA00599

Names & Taxonomyi

Protein namesi
Recommended name:
Probable diguanylate cyclase DgcTCurated (EC:2.7.7.651 Publication)
Short name:
DGC
Gene namesi
Name:dgcT1 Publication
Synonyms:ycdT
Ordered Locus Names:b1025, JW5143
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG13866 ycdT

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 7CytoplasmicCurated7
Transmembranei8 – 28HelicalSequence analysisAdd BLAST21
Topological domaini29 – 45PeriplasmicCuratedAdd BLAST17
Transmembranei46 – 66HelicalSequence analysisAdd BLAST21
Topological domaini67 – 74CytoplasmicCurated8
Transmembranei75 – 95HelicalSequence analysisAdd BLAST21
Topological domaini96 – 111PeriplasmicCuratedAdd BLAST16
Transmembranei112 – 132HelicalSequence analysisAdd BLAST21
Topological domaini133 – 148CytoplasmicCuratedAdd BLAST16
Transmembranei149 – 169HelicalSequence analysisAdd BLAST21
Topological domaini170 – 195PeriplasmicCuratedAdd BLAST26
Transmembranei196 – 216HelicalSequence analysisAdd BLAST21
Topological domaini217 – 219CytoplasmicCurated3
Transmembranei220 – 240HelicalSequence analysisAdd BLAST21
Topological domaini241 – 254PeriplasmicCuratedAdd BLAST14
Transmembranei255 – 275HelicalSequence analysisAdd BLAST21
Topological domaini276 – 452Cytoplasmic1 PublicationAdd BLAST177

GO - Cellular componenti

  • integral component of membrane Source: UniProtKB-KW
  • plasma membrane Source: EcoCyc

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

Pathology & Biotechi

Disruption phenotypei

A slight increase in motility. No visible effect on curli production.1 Publication

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi359 – 360GG → AA: Cells overexpressing this mutant are no longer swimming suppressed. 1 Publication2

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001688061 – 452Probable diguanylate cyclase DgcTAdd BLAST452

Proteomic databases

PaxDbiP75908
PRIDEiP75908

Expressioni

Inductioni

CsrA binds to the mRNA and reduces its levels. Expressed at low levels at both 28 and 37 degrees Celsius.2 Publications

Interactioni

Subunit structurei

Homodimer.By similarity

Protein-protein interaction databases

BioGridi4263227, 10 interactors
STRINGi316407.85674818

Structurei

3D structure databases

ProteinModelPortaliP75908
SMRiP75908
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini310 – 445GGDEFPROSITE-ProRule annotationAdd BLAST136

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG4105BZU Bacteria
ENOG410XNMH LUCA
HOGENOMiHOG000118907
InParanoidiP75908
OMAiWNGVSVI
PhylomeDBiP75908

Family and domain databases

CDDicd01949 GGDEF, 1 hit
InterProiView protein in InterPro
IPR000160 GGDEF_dom
IPR033424 MASE4
IPR029787 Nucleotide_cyclase
PfamiView protein in Pfam
PF00990 GGDEF, 1 hit
PF17158 MASE4, 1 hit
SMARTiView protein in SMART
SM00267 GGDEF, 1 hit
SUPFAMiSSF55073 SSF55073, 1 hit
TIGRFAMsiTIGR00254 GGDEF, 1 hit
PROSITEiView protein in PROSITE
PS50887 GGDEF, 1 hit

Sequencei

Sequence statusi: Complete.

P75908-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEKDYLRISS TVLVSLLFGL ALVLVNSWFN QPGVEEVVPR STYLMVMIAL
60 70 80 90 100
FFIDTVAFIF MQLYFIYDRR QFSNCVLSLA FLSCLIYFVI TVIIIQQIIE
110 120 130 140 150
ERLTSSVVQN DIAIYYLFRQ MSLCILIFLA LVNKVSENTK QRNLFSKKMT
160 170 180 190 200
LCISLFFVFG GPIVAHILSS HYESYNLHIA ELTNENGQVV WKASYVTIMI
210 220 230 240 250
FMWLTLLSVN LYFNGLRYDI WNGVTVIAFC AVLYNISLLF MSRYSVSTWY
260 270 280 290 300
ISRTIEVVSK LTVMVIFMCH IFSALRVTKN IAHRDPLTNI FNRNYFFNEL
310 320 330 340 350
TVQSASAQKT PYCVMIMDID HFKKVNDTWG HPVGDQVIKT VVNIIGKSIR
360 370 380 390 400
PDDLLARVGG EEFGVLLTDI DTERAKALAE RIRENVERLT GDNPEYAIPQ
410 420 430 440 450
KVTISIGAVV TQENALNPNE IYRLADNALY EAKETGRNKV VVRDVVNFCE

SP
Length:452
Mass (Da):51,783
Last modified:February 1, 1997 - v1
Checksum:iB6FB9E7EB894D701
GO

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural varianti130A → V in strain: K12 / W3110 / ATCC 27325 / DSM 5911. 1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00096 Genomic DNA Translation: AAC74110.1
AP009048 Genomic DNA Translation: BAA35810.2
PIRiG64844
RefSeqiNP_415544.1, NC_000913.3
WP_000409873.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaiAAC74110; AAC74110; b1025
BAA35810; BAA35810; BAA35810
GeneIDi945593
KEGGiecj:JW5143
eco:b1025
PATRICifig|511145.12.peg.1065

Similar proteinsi

Entry informationi

Entry nameiDGCT_ECOLI
AccessioniPrimary (citable) accession number: P75908
Secondary accession number(s): Q9R7P4, Q9R7P6
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: February 1, 1997
Last modified: March 28, 2018
This is version 127 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health