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Protein

Poly-beta-1,6-N-acetyl-D-glucosamine synthase

Gene

pgaC

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Probable N-acetylglucosaminyltransferase that catalyzes the polymerization of single monomer units of UDP-N-acetylglucosamine to produce the linear homopolymer poly-beta-1,6-N-acetyl-D-glucosamine (PGA), a biofilm adhesin polysaccharide.2 Publications

GO - Molecular functioni

  • acetylglucosaminyltransferase activity Source: EcoCyc

GO - Biological processi

  • cell adhesion involved in biofilm formation Source: EcoCyc

Keywordsi

Molecular functionGlycosyltransferase, Transferase

Enzyme and pathway databases

BioCyciEcoCyc:G6529-MONOMER
MetaCyc:G6529-MONOMER

Protein family/group databases

CAZyiGT2 Glycosyltransferase Family 2
TCDBi4.D.1.1.3 the putative vectorial glycosyl polymerization (vgp) family

Names & Taxonomyi

Protein namesi
Recommended name:
Poly-beta-1,6-N-acetyl-D-glucosamine synthase (EC:2.4.1.-)
Short name:
PGA synthase
Short name:
Poly-beta-1,6-GlcNAc synthase
Alternative name(s):
Biofilm PGA synthesis protein PgaC
N-acetylglucosaminyltransferase PgaC
Gene namesi
Name:pgaC
Synonyms:ycdQ
Ordered Locus Names:b1022, JW1007
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG13863 pgaC

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 4PeriplasmicSequence analysis4
Transmembranei5 – 25HelicalSequence analysisAdd BLAST21
Topological domaini26 – 32CytoplasmicSequence analysis7
Transmembranei33 – 53HelicalSequence analysisAdd BLAST21
Topological domaini54 – 330PeriplasmicSequence analysisAdd BLAST277
Transmembranei331 – 351HelicalSequence analysisAdd BLAST21
Topological domaini352 – 362CytoplasmicSequence analysisAdd BLAST11
Transmembranei363 – 383HelicalSequence analysisAdd BLAST21
Topological domaini384 – 394PeriplasmicSequence analysisAdd BLAST11
Transmembranei395 – 415HelicalSequence analysisAdd BLAST21
Topological domaini416 – 441CytoplasmicSequence analysisAdd BLAST26

GO - Cellular componenti

  • integral component of membrane Source: UniProtKB-KW
  • plasma membrane Source: EcoCyc

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

Pathology & Biotechi

Disruption phenotypei

Cells lacking this gene do not synthesize PGA.2 Publications

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000592121 – 441Poly-beta-1,6-N-acetyl-D-glucosamine synthaseAdd BLAST441

Proteomic databases

PaxDbiP75905
PRIDEiP75905

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
pgaDP694322EBI-561450,EBI-562069

Protein-protein interaction databases

BioGridi4260056, 253 interactors
DIPiDIP-11512N
IntActiP75905, 6 interactors
MINTiP75905
STRINGi316385.ECDH10B_1095

Structurei

3D structure databases

ProteinModelPortaliP75905
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyltransferase 2 family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG4105CND Bacteria
COG1215 LUCA
HOGENOMiHOG000170129
InParanoidiP75905
KOiK11936
OMAiGFGFVFF
PhylomeDBiP75905

Family and domain databases

Gene3Di3.90.550.10, 1 hit
InterProiView protein in InterPro
IPR001173 Glyco_trans_2-like
IPR029044 Nucleotide-diphossugar_trans
IPR023853 PGA_PgaC/IcaA
PfamiView protein in Pfam
PF00535 Glycos_transf_2, 1 hit
SUPFAMiSSF53448 SSF53448, 1 hit
TIGRFAMsiTIGR03937 PgaC_IcaA, 1 hit

Sequencei

Sequence statusi: Complete.

P75905-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MINRIVSFFI LCLVLCIPLC VAYFHSGELM MRFVFFWPFF MSIMWIVGGV
60 70 80 90 100
YFWVYRERHW PWGENAPAPQ LKDNPSISII IPCFNEEKNV EETIHAALAQ
110 120 130 140 150
RYENIEVIAV NDGSTDKTRA ILDRMAAQIP HLRVIHLAQN QGKAIALKTG
160 170 180 190 200
AAAAKSEYLV CIDGDALLDR DAAAYIVEPM LYNPRVGAVT GNPRIRTRST
210 220 230 240 250
LVGKIQVGEY SSIIGLIKRT QRIYGNVFTV SGVIAAFRRS ALAEVGYWSD
260 270 280 290 300
DMITEDIDIS WKLQLNQWTI FYEPRALCWI LMPETLKGLW KQRLRWAQGG
310 320 330 340 350
AEVFLKNMTR LWRKENFRMW PLFFEYCLTT IWAFTCLVGF IIYAVQLAGV
360 370 380 390 400
PLNIELTHIA ATHTAGILLC TLCLLQFIVS LMIENRYEHN LTSSLFWIIW
410 420 430 440
FPVIFWMLSL ATTLVSFTRV MLMPKKQRAR WVSPDRGILR G
Length:441
Mass (Da):50,766
Last modified:February 1, 1997 - v1
Checksum:i04F5A53D72FEBABB
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00096 Genomic DNA Translation: AAC74107.1
AP009048 Genomic DNA Translation: BAA35803.1
PIRiD64844
RefSeqiNP_415541.1, NC_000913.3
WP_000610451.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaiAAC74107; AAC74107; b1022
BAA35803; BAA35803; BAA35803
GeneIDi945606
KEGGiecj:JW1007
eco:b1022
PATRICifig|1411691.4.peg.1247

Similar proteinsi

Entry informationi

Entry nameiPGAC_ECOLI
AccessioniPrimary (citable) accession number: P75905
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: February 1, 1997
Last modified: March 28, 2018
This is version 129 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families

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