P75905 (PGAC_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Poly-beta-1,6-N-acetyl-D-glucosamine synthase
Short name=PGA synthase
Short name=Poly-beta-1,6-GlcNAc synthase
EC=2.4.1.-

Alternative name(s):
Biofilm PGA synthesis protein PgaC
N-acetylglucosaminyltransferase PgaC

Gene names

Name:	pgaC
Synonyms:	ycdQ
Ordered Locus Names:	b1022, JW1007

Organism

Escherichia coli (strain K12) [Reference proteome] [HAMAP]

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia › Escherichia coli

Protein attributes

Sequence length	441 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Probable N-acetylglucosaminyltransferase that catalyzes the polymerization of single monomer units of UDP-N-acetylglucosamine to produce the linear homopolymer poly-beta-1,6-N-acetyl-D-glucosamine (PGA), a biofilm adhesin polysaccharide. Ref.4 Ref.6
Subcellular location	Cell inner membrane; Multi-pass membrane protein.
Disruption phenotype	Cells lacking this gene do not synthesize PGA. Ref.4 Ref.6
Sequence similarities	Belongs to the glycosyltransferase 2 family.

Ontologies

Keywords
Cellular component	Cell inner membrane Cell membrane Membrane
Domain	Transmembrane Transmembrane helix
Molecular function	Glycosyltransferase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	cell adhesion involved in biofilm formation Inferred from mutant phenotype Ref.4. Source: EcoCyc
Cellular_component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	acetylglucosaminyltransferase activity Inferred from mutant phenotype Ref.6. Source: EcoCyc
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 441	441	Poly-beta-1,6-N-acetyl-D-glucosamine synthase		PRO_0000059212
Regions
	Topological domain	1 – 4	4	Periplasmic Potential
	Transmembrane	5 – 25	21	Helical; Potential
	Topological domain	26 – 32	7	Cytoplasmic Potential
	Transmembrane	33 – 53	21	Helical; Potential
	Topological domain	54 – 330	277	Periplasmic Potential
	Transmembrane	331 – 351	21	Helical; Potential
	Topological domain	352 – 362	11	Cytoplasmic Potential
	Transmembrane	363 – 383	21	Helical; Potential
	Topological domain	384 – 394	11	Periplasmic Potential
	Transmembrane	395 – 415	21	Helical; Potential
	Topological domain	416 – 441	26	Cytoplasmic Potential

Sequences

	Sequence		Length	Mass (Da)
	P75905 [UniParc]. Last modified February 1, 1997. Version 1. Checksum: 04F5A53D72FEBABB	FASTA	441	50,766
	10 20 30 40 50 60 MINRIVSFFI LCLVLCIPLC VAYFHSGELM MRFVFFWPFF MSIMWIVGGV YFWVYRERHW 70 80 90 100 110 120 PWGENAPAPQ LKDNPSISII IPCFNEEKNV EETIHAALAQ RYENIEVIAV NDGSTDKTRA 130 140 150 160 170 180 ILDRMAAQIP HLRVIHLAQN QGKAIALKTG AAAAKSEYLV CIDGDALLDR DAAAYIVEPM 190 200 210 220 230 240 LYNPRVGAVT GNPRIRTRST LVGKIQVGEY SSIIGLIKRT QRIYGNVFTV SGVIAAFRRS 250 260 270 280 290 300 ALAEVGYWSD DMITEDIDIS WKLQLNQWTI FYEPRALCWI LMPETLKGLW KQRLRWAQGG 310 320 330 340 350 360 AEVFLKNMTR LWRKENFRMW PLFFEYCLTT IWAFTCLVGF IIYAVQLAGV PLNIELTHIA 370 380 390 400 410 420 ATHTAGILLC TLCLLQFIVS LMIENRYEHN LTSSLFWIIW FPVIFWMLSL ATTLVSFTRV 430 440 MLMPKKQRAR WVSPDRGILR G

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"A 718-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 12.7-28.0 min region on the linkage map." Oshima T., Aiba H., Baba T., Fujita K., Hayashi K., Honjo A., Ikemoto K., Inada T., Itoh T., Kajihara M., Kanai K., Kashimoto K., Kimura S., Kitagawa M., Makino K., Masuda S., Miki T., Mizobuchi K. , Mori H., Motomura K., Nakamura Y., Nashimoto H., Nishio Y., Saito N., Sampei G., Seki Y., Tagami H., Takemoto K., Wada C., Yamamoto Y., Yano M., Horiuchi T. DNA Res. 3:137-155(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[2]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[3]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[4]	"The pgaABCD locus of Escherichia coli promotes the synthesis of a polysaccharide adhesin required for biofilm formation." Wang X., Preston J.F. III, Romeo T. J. Bacteriol. 186:2724-2734(2004) [PubMed] [Europe PMC] [Abstract] Cited for: ROLE IN THE SYNTHESIS OF A BIOFILM POLYSACCHARIDE, OPERON STRUCTURE, DISRUPTION PHENOTYPE. Strain: K12 / MG1655 / ATCC 47076.
[5]	"Global topology analysis of the Escherichia coli inner membrane proteome." Daley D.O., Rapp M., Granseth E., Melen K., Drew D., von Heijne G. Science 308:1321-1323(2005) [PubMed] [Europe PMC] [Abstract] Cited for: TOPOLOGY [LARGE SCALE ANALYSIS], SUBCELLULAR LOCATION. Strain: K12 / MG1655 / ATCC 47076.
[6]	"Roles of pgaABCD genes in synthesis, modification, and export of the Escherichia coli biofilm adhesin poly-beta-1,6-N-acetyl-D-glucosamine." Itoh Y., Rice J.D., Goller C., Pannuri A., Taylor J., Meisner J., Beveridge T.J., Preston J.F. III, Romeo T. J. Bacteriol. 190:3670-3680(2008) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION IN PGA SYNTHESIS, DISRUPTION PHENOTYPE. Strain: K12 / MG1655 / ATCC 47076.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U00096 Genomic DNA. Translation: AAC74107.1. AP009048 Genomic DNA. Translation: BAA35803.1.
PIR	D64844.
RefSeq	NP_415541.1. NC_000913.2. YP_489293.1. NC_007779.1.
3D structure databases
ProteinModelPortal	P75905.
SMR	P75905. Positions 67-334.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-11512N.
IntAct	P75905. 5 interactions.
MINT	MINT-1234361.
STRING	511145.b1022.
Protein family/group databases
CAZy	GT2. Glycosyltransferase Family 2.
TCDB	9.B.32.1.3. putative vectorial glycosyl polymerization (VGP) family.
Proteomic databases
PaxDb	P75905.
PRIDE	P75905.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAC74107; AAC74107; b1022. BAA35803; BAA35803; BAA35803.
GeneID	12931062. 945606.
KEGG	ecj:Y75_p0994. eco:b1022.
PATRIC	32117277. VBIEscCol129921_1062.
Organism-specific databases
EchoBASE	EB3623.
EcoGene	EG13863. pgaC.
Phylogenomic databases
eggNOG	COG1215.
HOGENOM	HOG000170129.
KO	K11936.
OMA	LVSFPKV.
ProtClustDB	PRK11204.
Enzyme and pathway databases
BioCyc	EcoCyc:G6529-MONOMER. ECOL316407:JW1007-MONOMER. MetaCyc:G6529-MONOMER.
Gene expression databases
Genevestigator	P75905.
Family and domain databases
InterPro	IPR001173. Glyco_trans_2. IPR023853. PGA_PgaC/IcaA. [Graphical view]
Pfam	PF00535. Glycos_transf_2. 1 hit. [Graphical view]
TIGRFAMs	TIGR03937. PgaC_IcaA. 1 hit.
ProtoNet	Search...

Entry information

Entry name

PGAC_ECOLI

Accession

Primary (citable) accession number: P75905

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	February 1, 1997
Last modified:	May 1, 2013
This is version 100 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program