P75901 (EFEU_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Putative inactive ferrous iron permease EfeU

Alternative name(s):
Putative Fe(2+) ion permease EfeU

Gene names

Name:	efeU
Synonyms:	ycdN
Ordered Locus Names:	b4490, JW5141/JW1002
ORF Names:	b1016/b1017

Organism

Escherichia coli (strain K12) [Reference proteome] [HAMAP]

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia › Escherichia coli

Protein attributes

Sequence length	276 AA.
Sequence status	Complete.
Protein existence	Uncertain

General annotation (Comments)

Subunit structure	Part of a ferrous iron transporter composed of EfeU, EfeO and EfeB. However, this EfeUOB tripartite iron transporter is defective in E.coli strain K12 due to a frameshift mutation in EfeU. Ref.4
Subcellular location	Cell inner membrane; Multi-pass membrane protein By similarity.
Sequence similarities	Belongs to the oxidase-dependent Fe transporter (OFeT) (TC 9.A.10.1) family. [View classification]
Caution	Could be the product of a pseudogene. Strain K12 contains a truncated, inactive permease due to a frameshift in position 34 that produces two separate ORFs. The sequence shown here is a reconstruction originating from EcoGene.
Sequence caution	The sequence AP009048 differs from that shown. Reason: Frameshift at position 34. The sequence U00096 differs from that shown. Reason: Frameshift at position 34.

Ontologies

Keywords
Cellular component	Cell inner membrane Cell membrane Membrane
Domain	Transmembrane Transmembrane helix
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Cellular_component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ferrous iron uptake transmembrane transporter activity Inferred from mutant phenotype Ref.4. Source: EcoCyc
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 276	276	Putative inactive ferrous iron permease EfeU		PRO_0000159652
Regions
	Topological domain	1	1	Periplasmic Potential
	Transmembrane	2 – 22	21	Helical; Potential
	Topological domain	23 – 35	13	Cytoplasmic Potential
	Transmembrane	36 – 56	21	Helical; Potential
	Topological domain	57 – 69	13	Periplasmic Potential
	Transmembrane	70 – 90	21	Helical; Potential
	Topological domain	91 – 118	28	Cytoplasmic Potential
	Transmembrane	119 – 139	21	Helical; Potential
	Topological domain	140 – 147	8	Periplasmic Potential
	Transmembrane	148 – 168	21	Helical; Potential
	Topological domain	169 – 179	11	Cytoplasmic Potential
	Transmembrane	180 – 200	21	Helical; Potential
	Topological domain	201 – 244	44	Periplasmic Potential
	Transmembrane	245 – 265	21	Helical; Potential
	Topological domain	266 – 276	11	Cytoplasmic Potential

Sequences

	Sequence		Length	Mass (Da)
	P75901 [UniParc]. Last modified December 15, 1998. Version 2. Checksum: 27FC648B7F791AE7	FASTA	276	30,292
	10 20 30 40 50 60 MFVPFLIMLR EGLEAALIVS LIASYLKRTQ RGMXDCVMWI GVLLAAALCL GLGIFINETT 70 80 90 100 110 120 GEFPQKEQEL FEGIVAVIAV VILTWMVFWM RKVSRNVKVQ LEQAVDSALQ RGNHHGWALV 130 140 150 160 170 180 MMVFFAVARE GLESVFFLLA AFQQDVGIWP PLGAMLGLAT AVVLGFLLYW GGIRLNLGAF 190 200 210 220 230 240 FKWTSLFILF VAAGLAAGAI RAFHEAGLWN HFQEIAFDMS AVLSTHSLFG TLMEGIFGYQ 250 260 270 EAPSVSEVAV WFIYLIPALV AFALPPRAGA TASRSA

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"A 718-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 12.7-28.0 min region on the linkage map." Oshima T., Aiba H., Baba T., Fujita K., Hayashi K., Honjo A., Ikemoto K., Inada T., Itoh T., Kajihara M., Kanai K., Kashimoto K., Kimura S., Kitagawa M., Makino K., Masuda S., Miki T., Mizobuchi K. , Mori H., Motomura K., Nakamura Y., Nashimoto H., Nishio Y., Saito N., Sampei G., Seki Y., Tagami H., Takemoto K., Wada C., Yamamoto Y., Yano M., Horiuchi T. DNA Res. 3:137-155(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[2]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[3]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[4]	"EfeUOB (YcdNOB) is a tripartite, acid-induced and CpxAR-regulated, low-pH Fe2+ transporter that is cryptic in Escherichia coli K-12 but functional in E. coli O157:H7." Cao J., Woodhall M.R., Alvarez J., Cartron M.L., Andrews S.C. Mol. Microbiol. 65:857-875(2007) [PubMed] [Europe PMC] [Abstract] Cited for: SUBUNIT, LACK OF ACTIVITY IN STRAIN K12. Strain: K12.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U00096 Genomic DNA. No translation available. AP009048 Genomic DNA. No translation available.
3D structure databases
ProteinModelPortal	P75901.
ModBase	Search...
Protein family/group databases
TCDB	9.A.10.2.3. iron/lead transporter (ILT) superfamily.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Organism-specific databases
EchoBASE	EB3620.
EcoGene	EG13860. efeU.
Phylogenomic databases
HOGENOM	HOG000236918.
Enzyme and pathway databases
BioCyc	EcoCyc:G6525-MONOMER.
Gene expression databases
Genevestigator	P75901.
Family and domain databases
InterPro	IPR005217. CHP00145. IPR004923. FTR1. [Graphical view]
Pfam	PF03239. FTR1. 1 hit. [Graphical view]
TIGRFAMs	TIGR00145. TIGR00145. 1 hit.
ProtoNet	Search...

Entry information

Entry name

EFEU_ECOLI

Accession

Primary (citable) accession number: P75901
Secondary accession number(s): P75900, Q9R3S6

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 15, 1998
Last sequence update:	December 15, 1998
Last modified:	May 1, 2013
This is version 94 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program