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Protein

Peroxyureidoacrylate/ureidoacrylate amidohydrolase RutB

Gene

rutB

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

In vivo, quickly hydrolyzes the ureidoacrylate peracid to avoid toxicity, but can also hydrolyzes ureidoacrylate that is formed spontaneously from ureidoacrylate peracid. One of the products of hydrolysis, carbamate, hydrolyzes spontaneously, thereby releasing one of the pyrimidine rings nitrogen atoms as ammonia and one of its carbons as CO2.2 Publications

Catalytic activityi

(Z)-3-ureidoacrylate peracid + H2O = (Z)-3-peroxyaminoacrylate + CO2 + NH3.1 Publication
(Z)-3-ureidoacrylate + H2O = (Z)-3-aminoacrylate + CO2 + NH3.1 Publication
(Z)-2-methyl-ureidoacrylate peracid + H2O = (Z)-2-methyl-peroxyaminoacrylate + CO2 + NH3.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei166NucleophileBy similarity1

GO - Molecular functioni

GO - Biological processi

  • nitrogen utilization Source: UniProtKB
  • pyrimidine nucleobase catabolic process Source: EcoCyc
  • uracil catabolic process Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Enzyme and pathway databases

BioCyciEcoCyc:G6522-MONOMER.
ECOL316407:JW5139-MONOMER.
MetaCyc:G6522-MONOMER.
BRENDAi3.5.1.110. 2026.

Names & Taxonomyi

Protein namesi
Recommended name:
Peroxyureidoacrylate/ureidoacrylate amidohydrolase RutB (EC:3.5.1.110)
Alternative name(s):
Ureidoacrylate amidohydrolase
Gene namesi
Name:rutB
Synonyms:ycdL
Ordered Locus Names:b1011, JW5139
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG13858. rutB.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Pathology & Biotechi

Disruption phenotypei

Cells lacking this gene fail to grow on uridine as the sole source of nitrogen at room temperature.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002018331 – 230Peroxyureidoacrylate/ureidoacrylate amidohydrolase RutBAdd BLAST230

Proteomic databases

PaxDbiP75897.
PRIDEiP75897.

Expressioni

Inductioni

Up-regulated by the nitrogen regulatory protein C (NtrC also called GlnG) and repressed by RutR.2 Publications

Interactioni

Protein-protein interaction databases

BioGridi4261997. 1 interactor.
DIPiDIP-28106N.
STRINGi511145.b1011.

Structurei

3D structure databases

ProteinModelPortaliP75897.
SMRiP75897.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the isochorismatase family. RutB subfamily.Curated

Phylogenomic databases

eggNOGiENOG4106XMA. Bacteria.
COG1335. LUCA.
HOGENOMiHOG000078669.
InParanoidiP75897.
KOiK09020.
OMAiLAKGGWD.
PhylomeDBiP75897.

Family and domain databases

Gene3Di3.40.50.850. 1 hit.
HAMAPiMF_00830. RutB. 1 hit.
InterProiIPR019916. IsoChorase_RutB.
IPR000868. Isochorismatase-like.
[Graphical view]
PfamiPF00857. Isochorismatase. 1 hit.
[Graphical view]
SUPFAMiSSF52499. SSF52499. 1 hit.
TIGRFAMsiTIGR03614. RutB. 1 hit.

Sequencei

Sequence statusi: Complete.

P75897-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTTLTARPEA ITFDPQQSAL IVVDMQNAYA TPGGYLDLAG FDVSTTRPVI
60 70 80 90 100
ANIQTAVTAA RAAGMLIIWF QNGWDEQYVE AGGPGSPNFH KSNALKTMRK
110 120 130 140 150
QPQLQGKLLA KGSWDYQLVD ELVPQPGDIV LPKPRYSGFF NTPLDSILRS
160 170 180 190 200
RGIRHLVFTG IATNVCVEST LRDGFFLEYF GVVLEDATHQ AGPKFAQKAA
210 220 230
LFNIETFFGW VSDVETFCDA LSPTSFAHIA
Length:230
Mass (Da):25,209
Last modified:March 27, 2002 - v2
Checksum:i6E265222D32A6E80
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00096 Genomic DNA. Translation: AAC74096.2.
AP009048 Genomic DNA. Translation: BAA35778.1.
PIRiA64843.
RefSeqiNP_415531.2. NC_000913.3.
WP_001393558.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC74096; AAC74096; b1011.
BAA35778; BAA35778; BAA35778.
GeneIDi945699.
KEGGiecj:JW5139.
eco:b1011.
PATRICi32117251. VBIEscCol129921_1049.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00096 Genomic DNA. Translation: AAC74096.2.
AP009048 Genomic DNA. Translation: BAA35778.1.
PIRiA64843.
RefSeqiNP_415531.2. NC_000913.3.
WP_001393558.1. NZ_LN832404.1.

3D structure databases

ProteinModelPortaliP75897.
SMRiP75897.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4261997. 1 interactor.
DIPiDIP-28106N.
STRINGi511145.b1011.

Proteomic databases

PaxDbiP75897.
PRIDEiP75897.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC74096; AAC74096; b1011.
BAA35778; BAA35778; BAA35778.
GeneIDi945699.
KEGGiecj:JW5139.
eco:b1011.
PATRICi32117251. VBIEscCol129921_1049.

Organism-specific databases

EchoBASEiEB3618.
EcoGeneiEG13858. rutB.

Phylogenomic databases

eggNOGiENOG4106XMA. Bacteria.
COG1335. LUCA.
HOGENOMiHOG000078669.
InParanoidiP75897.
KOiK09020.
OMAiLAKGGWD.
PhylomeDBiP75897.

Enzyme and pathway databases

BioCyciEcoCyc:G6522-MONOMER.
ECOL316407:JW5139-MONOMER.
MetaCyc:G6522-MONOMER.
BRENDAi3.5.1.110. 2026.

Miscellaneous databases

PROiP75897.

Family and domain databases

Gene3Di3.40.50.850. 1 hit.
HAMAPiMF_00830. RutB. 1 hit.
InterProiIPR019916. IsoChorase_RutB.
IPR000868. Isochorismatase-like.
[Graphical view]
PfamiPF00857. Isochorismatase. 1 hit.
[Graphical view]
SUPFAMiSSF52499. SSF52499. 1 hit.
TIGRFAMsiTIGR03614. RutB. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiRUTB_ECOLI
AccessioniPrimary (citable) accession number: P75897
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 27, 2002
Last sequence update: March 27, 2002
Last modified: November 2, 2016
This is version 111 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Miscellaneous

The Rut pathway degrades exogenous pyrimidines as the sole nitrogen source at room temperature but not at 37 degrees Celsius, a restriction that is apparently a consequence of an inadequate ability to remove toxic malonic semialdehyde at the higher temperature (RutE/YdfG function).

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.