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Protein

FMN reductase (NADH) RutF

Gene

rutF

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes the reduction of FMN to FMNH2 which is used to reduce pyrimidine by RutA via the Rut pathway. In vitro, the flavin reductase Fre can substitute for the function of RutF, however, RutF is required for uracil utilization in vivo.2 Publications

Miscellaneous

The Rut pathway degrades exogenous pyrimidines as the sole nitrogen source at room temperature but not at 37 degrees Celsius, a restriction that is apparently a consequence of an inadequate ability to remove toxic malonic semialdehyde at the higher temperature (RutE/YdfG function).

Catalytic activityi

FMNH2 + NAD+ = FMN + NADH.1 Publication

GO - Molecular functioni

  • FMN binding Source: InterPro
  • FMN reductase activity Source: InterPro
  • riboflavin reductase (NADPH) activity Source: UniProtKB

GO - Biological processi

  • nitrogen utilization Source: UniProtKB
  • pyrimidine nucleobase catabolic process Source: EcoCyc
  • uracil catabolic process Source: UniProtKB

Keywordsi

Molecular functionOxidoreductase
LigandFlavoprotein, FMN, NAD

Enzyme and pathway databases

BioCyciEcoCyc:G6518-MONOMER
MetaCyc:G6518-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
FMN reductase (NADH) RutF (EC:1.5.1.42)
Alternative name(s):
FMN reductase
NADH-flavin reductase RutF
NADH:flavin oxidoreductase
Gene namesi
Name:rutF
Synonyms:ycdH
Ordered Locus Names:b1007, JW5138
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG13854 rutF

Pathology & Biotechi

Disruption phenotypei

Cells lacking this gene fail to grow on uridine as the sole source of nitrogen at room temperature.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000855371 – 164FMN reductase (NADH) RutFAdd BLAST164

Proteomic databases

PaxDbiP75893
PRIDEiP75893

Expressioni

Inductioni

Up-regulated by the nitrogen regulatory protein C (NtrC also called GlnG) and repressed by RutR.2 Publications

Interactioni

Protein-protein interaction databases

BioGridi4259548, 7 interactors
STRINGi316385.ECDH10B_1079

Structurei

3D structure databases

ProteinModelPortaliP75893
SMRiP75893
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiENOG4107YAQ Bacteria
COG1853 LUCA
HOGENOMiHOG000115783
InParanoidiP75893
KOiK09024
OMAiWFDRGYH
PhylomeDBiP75893

Family and domain databases

Gene3Di2.30.110.10, 1 hit
HAMAPiMF_00833 RutF, 1 hit
InterProiView protein in InterPro
IPR002563 Flavin_Rdtase-like_dom
IPR019917 RutF
IPR012349 Split_barrel_FMN-bd
PfamiView protein in Pfam
PF01613 Flavin_Reduct, 1 hit
SMARTiView protein in SMART
SM00903 Flavin_Reduct, 1 hit
TIGRFAMsiTIGR03615 RutF, 1 hit

Sequencei

Sequence statusi: Complete.

P75893-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNIVDQQTFR DAMSCMGAAV NIITTDGPAG RAGFTASAVC SVTDTPPTLL
60 70 80 90 100
VCLNRGASVW PAFNENRTLC VNTLSAGQEP LSNLFGGKTP MEHRFAAARW
110 120 130 140 150
QTGVTGCPQL EEALVSFDCR ISQVVSVGTH DILFCAIEAI HRHTTPYGLV
160
WFDRSYHALM RPAC
Length:164
Mass (Da):17,749
Last modified:May 2, 2002 - v3
Checksum:i70C0FDFF45183764
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00096 Genomic DNA Translation: AAC74092.2
AP009048 Genomic DNA Translation: BAA35774.2
PIRiE64842
RefSeqiNP_415527.4, NC_000913.3
WP_001028083.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaiAAC74092; AAC74092; b1007
BAA35774; BAA35774; BAA35774
GeneIDi946594
KEGGiecj:JW5138
eco:b1007
PATRICifig|1411691.4.peg.1264

Similar proteinsi

Entry informationi

Entry nameiRUTF_ECOLI
AccessioniPrimary (citable) accession number: P75893
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 2, 2002
Last sequence update: May 2, 2002
Last modified: March 28, 2018
This is version 118 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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