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Protein

FMN reductase (NADH) RutF

Gene

rutF

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the reduction of FMN to FMNH2 which is used to reduce pyrimidine by RutA via the Rut pathway. In vitro, the flavin reductase Fre can substitute for the function of RutF, however, RutF is required for uracil utilization in vivo.2 Publications

Catalytic activityi

FMNH2 + NAD+ = FMN + NADH.1 Publication

GO - Molecular functioni

  • FMN binding Source: InterPro
  • FMN reductase activity Source: InterPro
  • riboflavin reductase (NADPH) activity Source: UniProtKB

GO - Biological processi

  • nitrogen utilization Source: UniProtKB
  • pyrimidine nucleobase catabolic process Source: EcoCyc
  • uracil catabolic process Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Ligandi

Flavoprotein, FMN, NAD

Enzyme and pathway databases

BioCyciEcoCyc:G6518-MONOMER.
ECOL316407:JW5138-MONOMER.
MetaCyc:G6518-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
FMN reductase (NADH) RutF (EC:1.5.1.42)
Alternative name(s):
FMN reductase
NADH-flavin reductase RutF
NADH:flavin oxidoreductase
Gene namesi
Name:rutF
Synonyms:ycdH
Ordered Locus Names:b1007, JW5138
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG13854. rutF.

Pathology & Biotechi

Disruption phenotypei

Cells lacking this gene fail to grow on uridine as the sole source of nitrogen at room temperature.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 164164FMN reductase (NADH) RutFPRO_0000085537Add
BLAST

Proteomic databases

PaxDbiP75893.

Expressioni

Inductioni

Up-regulated by the nitrogen regulatory protein C (NtrC also called GlnG) and repressed by RutR.2 Publications

Interactioni

Protein-protein interaction databases

BioGridi4259548. 7 interactions.
STRINGi511145.b1007.

Structurei

3D structure databases

ProteinModelPortaliP75893.
SMRiP75893. Positions 4-159.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiENOG4107YAQ. Bacteria.
COG1853. LUCA.
HOGENOMiHOG000115783.
InParanoidiP75893.
KOiK09024.
OMAiWFDRGYH.
PhylomeDBiP75893.

Family and domain databases

Gene3Di2.30.110.10. 1 hit.
HAMAPiMF_00833. RutF. 1 hit.
InterProiIPR002563. Flavin_Rdtase-like_dom.
IPR019917. RutF.
IPR012349. Split_barrel_FMN-bd.
[Graphical view]
PfamiPF01613. Flavin_Reduct. 1 hit.
[Graphical view]
SMARTiSM00903. Flavin_Reduct. 1 hit.
[Graphical view]
SUPFAMiSSF50475. SSF50475. 1 hit.
TIGRFAMsiTIGR03615. RutF. 1 hit.

Sequencei

Sequence statusi: Complete.

P75893-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNIVDQQTFR DAMSCMGAAV NIITTDGPAG RAGFTASAVC SVTDTPPTLL
60 70 80 90 100
VCLNRGASVW PAFNENRTLC VNTLSAGQEP LSNLFGGKTP MEHRFAAARW
110 120 130 140 150
QTGVTGCPQL EEALVSFDCR ISQVVSVGTH DILFCAIEAI HRHTTPYGLV
160
WFDRSYHALM RPAC
Length:164
Mass (Da):17,749
Last modified:May 2, 2002 - v3
Checksum:i70C0FDFF45183764
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00096 Genomic DNA. Translation: AAC74092.2.
AP009048 Genomic DNA. Translation: BAA35774.2.
PIRiE64842.
RefSeqiNP_415527.4. NC_000913.3.
WP_001028083.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC74092; AAC74092; b1007.
BAA35774; BAA35774; BAA35774.
GeneIDi946594.
KEGGiecj:JW5138.
eco:b1007.
PATRICi32117243. VBIEscCol129921_1045.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00096 Genomic DNA. Translation: AAC74092.2.
AP009048 Genomic DNA. Translation: BAA35774.2.
PIRiE64842.
RefSeqiNP_415527.4. NC_000913.3.
WP_001028083.1. NZ_LN832404.1.

3D structure databases

ProteinModelPortaliP75893.
SMRiP75893. Positions 4-159.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4259548. 7 interactions.
STRINGi511145.b1007.

Proteomic databases

PaxDbiP75893.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC74092; AAC74092; b1007.
BAA35774; BAA35774; BAA35774.
GeneIDi946594.
KEGGiecj:JW5138.
eco:b1007.
PATRICi32117243. VBIEscCol129921_1045.

Organism-specific databases

EchoBASEiEB3614.
EcoGeneiEG13854. rutF.

Phylogenomic databases

eggNOGiENOG4107YAQ. Bacteria.
COG1853. LUCA.
HOGENOMiHOG000115783.
InParanoidiP75893.
KOiK09024.
OMAiWFDRGYH.
PhylomeDBiP75893.

Enzyme and pathway databases

BioCyciEcoCyc:G6518-MONOMER.
ECOL316407:JW5138-MONOMER.
MetaCyc:G6518-MONOMER.

Miscellaneous databases

PROiP75893.

Family and domain databases

Gene3Di2.30.110.10. 1 hit.
HAMAPiMF_00833. RutF. 1 hit.
InterProiIPR002563. Flavin_Rdtase-like_dom.
IPR019917. RutF.
IPR012349. Split_barrel_FMN-bd.
[Graphical view]
PfamiPF01613. Flavin_Reduct. 1 hit.
[Graphical view]
SMARTiSM00903. Flavin_Reduct. 1 hit.
[Graphical view]
SUPFAMiSSF50475. SSF50475. 1 hit.
TIGRFAMsiTIGR03615. RutF. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiRUTF_ECOLI
AccessioniPrimary (citable) accession number: P75893
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 2, 2002
Last sequence update: May 2, 2002
Last modified: September 7, 2016
This is version 111 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Miscellaneous

The Rut pathway degrades exogenous pyrimidines as the sole nitrogen source at room temperature but not at 37 degrees Celsius, a restriction that is apparently a consequence of an inadequate ability to remove toxic malonic semialdehyde at the higher temperature (RutE/YdfG function).

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.