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Protein

Putative pyrimidine permease RutG

Gene

rutG

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

May function as a proton-driven pyrimidine uptake system.1 Publication

GO - Molecular functioni

  • pyrimidine nucleotide transmembrane transporter activity Source: InterPro
  • symporter activity Source: UniProtKB-KW
  • uracil transmembrane transporter activity Source: EcoCyc

GO - Biological processi

  • uracil catabolic process Source: EcoCyc
  • uracil transport Source: EcoCyc
Complete GO annotation...

Keywords - Biological processi

Symport, Transport

Enzyme and pathway databases

BioCyciEcoCyc:B1006-MONOMER.
ECOL316407:JW5137-MONOMER.

Protein family/group databases

TCDBi2.A.40.1.3. the nucleobase/ascorbate transporter (nat) or nucleobase:cation symporter-2 (ncs2) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Putative pyrimidine permease RutG
Gene namesi
Name:rutG
Synonyms:ycdG
Ordered Locus Names:b1006, JW5137
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG13853. rutG.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 5757CytoplasmicSequence analysisAdd
BLAST
Transmembranei58 – 7821HelicalSequence analysisAdd
BLAST
Topological domaini79 – 791PeriplasmicSequence analysis
Transmembranei80 – 10021HelicalSequence analysisAdd
BLAST
Topological domaini101 – 11010CytoplasmicSequence analysis
Transmembranei111 – 13121HelicalSequence analysisAdd
BLAST
Topological domaini132 – 1409PeriplasmicSequence analysis
Transmembranei141 – 16121HelicalSequence analysisAdd
BLAST
Topological domaini162 – 1698CytoplasmicSequence analysis
Transmembranei170 – 19021HelicalSequence analysisAdd
BLAST
Topological domaini191 – 1966PeriplasmicSequence analysis
Transmembranei197 – 21721HelicalSequence analysisAdd
BLAST
Topological domaini218 – 24023CytoplasmicSequence analysisAdd
BLAST
Transmembranei241 – 26121HelicalSequence analysisAdd
BLAST
Topological domaini262 – 28423PeriplasmicSequence analysisAdd
BLAST
Transmembranei285 – 30521HelicalSequence analysisAdd
BLAST
Topological domaini306 – 31813CytoplasmicSequence analysisAdd
BLAST
Transmembranei319 – 33921HelicalSequence analysisAdd
BLAST
Topological domaini340 – 3478PeriplasmicSequence analysis
Transmembranei348 – 36821HelicalSequence analysisAdd
BLAST
Topological domaini369 – 38517CytoplasmicSequence analysisAdd
BLAST
Transmembranei386 – 40621HelicalSequence analysisAdd
BLAST
Transmembranei407 – 42721HelicalSequence analysisAdd
BLAST
Topological domaini428 – 44215CytoplasmicSequence analysisAdd
BLAST

GO - Cellular componenti

  • integral component of plasma membrane Source: EcoCyc
  • plasma membrane Source: EcoCyc
Complete GO annotation...

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

Pathology & Biotechi

Disruption phenotypei

Cells lacking this gene fail to grow on the nucleobase uracil as the sole nitrogen source but use the nucleoside uridine normally.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 442442Putative pyrimidine permease RutGPRO_0000165964Add
BLAST

Proteomic databases

PaxDbiP75892.

Expressioni

Inductioni

Up-regulated by the nitrogen regulatory protein C (NtrC also called GlnG) and repressed by RutR.2 Publications

Interactioni

Protein-protein interaction databases

BioGridi4259546. 6 interactions.
STRINGi511145.b1006.

Structurei

3D structure databases

ProteinModelPortaliP75892.
SMRiP75892. Positions 21-425.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG4105C2W. Bacteria.
COG2233. LUCA.
HOGENOMiHOG000038200.
InParanoidiP75892.
KOiK09016.
OMAiRLTWTRT.
PhylomeDBiP75892.

Family and domain databases

InterProiIPR019918. Pyrimidine_permease_RutG_pred.
IPR006042. Xan_ur_permease.
IPR006043. Xant/urac/vitC.
[Graphical view]
PANTHERiPTHR11119. PTHR11119. 1 hit.
PfamiPF00860. Xan_ur_permease. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00801. ncs2. 1 hit.
TIGR03616. RutG. 1 hit.
PROSITEiPS01116. XANTH_URACIL_PERMASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P75892-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAMFGFPHWQ LKSTSTESGV VAPDERLPFA QTAVMGVQHA VAMFGATVLM
60 70 80 90 100
PILMGLDPNL SILMSGIGTL LFFFITGGRV PSYLGSSAAF VGVVIAATGF
110 120 130 140 150
NGQGINPNIS IALGGIIACG LVYTVIGLVV MKIGTRWIER LMPPVVTGAV
160 170 180 190 200
VMAIGLNLAP IAVKSVSASA FDSWMAVMTV LCIGLVAVFT RGMIQRLLIL
210 220 230 240 250
VGLIVACLLY GVMTNVLGLG KAVDFTLVSH AAWFGLPHFS TPAFNGQAMM
260 270 280 290 300
LIAPVAVILV AENLGHLKAV AGMTGRNMDP YMGRAFVGDG LATMLSGSVG
310 320 330 340 350
GSGVTTYAEN IGVMAVTKVY STLVFVAAAV IAMLLGFSPK FGALIHTIPA
360 370 380 390 400
AVIGGASIVV FGLIAVAGAR IWVQNRVDLS QNGNLIMVAV TLVLGAGDFA
410 420 430 440
LTLGGFTLGG IGTATFGAIL LNALLSRKLV DVPPPEVVHQ EP
Length:442
Mass (Da):45,557
Last modified:December 1, 2000 - v2
Checksum:i35CC612A4E86DD67
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00096 Genomic DNA. Translation: AAC74091.2.
AP009048 Genomic DNA. Translation: BAA35773.1.
PIRiD64842.
RefSeqiNP_415526.4. NC_000913.3.
WP_001326838.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC74091; AAC74091; b1006.
BAA35773; BAA35773; BAA35773.
GeneIDi946589.
KEGGiecj:JW5137.
eco:b1006.
PATRICi32117241. VBIEscCol129921_1044.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00096 Genomic DNA. Translation: AAC74091.2.
AP009048 Genomic DNA. Translation: BAA35773.1.
PIRiD64842.
RefSeqiNP_415526.4. NC_000913.3.
WP_001326838.1. NZ_LN832404.1.

3D structure databases

ProteinModelPortaliP75892.
SMRiP75892. Positions 21-425.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4259546. 6 interactions.
STRINGi511145.b1006.

Protein family/group databases

TCDBi2.A.40.1.3. the nucleobase/ascorbate transporter (nat) or nucleobase:cation symporter-2 (ncs2) family.

Proteomic databases

PaxDbiP75892.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC74091; AAC74091; b1006.
BAA35773; BAA35773; BAA35773.
GeneIDi946589.
KEGGiecj:JW5137.
eco:b1006.
PATRICi32117241. VBIEscCol129921_1044.

Organism-specific databases

EchoBASEiEB3613.
EcoGeneiEG13853. rutG.

Phylogenomic databases

eggNOGiENOG4105C2W. Bacteria.
COG2233. LUCA.
HOGENOMiHOG000038200.
InParanoidiP75892.
KOiK09016.
OMAiRLTWTRT.
PhylomeDBiP75892.

Enzyme and pathway databases

BioCyciEcoCyc:B1006-MONOMER.
ECOL316407:JW5137-MONOMER.

Miscellaneous databases

PROiP75892.

Family and domain databases

InterProiIPR019918. Pyrimidine_permease_RutG_pred.
IPR006042. Xan_ur_permease.
IPR006043. Xant/urac/vitC.
[Graphical view]
PANTHERiPTHR11119. PTHR11119. 1 hit.
PfamiPF00860. Xan_ur_permease. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00801. ncs2. 1 hit.
TIGR03616. RutG. 1 hit.
PROSITEiPS01116. XANTH_URACIL_PERMASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiRUTG_ECOLI
AccessioniPrimary (citable) accession number: P75892
Secondary accession number(s): Q9R3W5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: December 1, 2000
Last modified: September 7, 2016
This is version 122 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Miscellaneous

The Rut pathway degrades exogenous pyrimidines as the sole nitrogen source at room temperature but not at 37 degrees Celsius, a restriction that is apparently a consequence of an inadequate ability to remove toxic malonic semialdehyde at the higher temperature (RutE/YdfG function).

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.