Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Putative pyrimidine permease RutG

Gene

rutG

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

May function as a proton-driven pyrimidine uptake system.1 Publication

Miscellaneous

The Rut pathway degrades exogenous pyrimidines as the sole nitrogen source at room temperature but not at 37 degrees Celsius, a restriction that is apparently a consequence of an inadequate ability to remove toxic malonic semialdehyde at the higher temperature (RutE/YdfG function).

GO - Molecular functioni

  • pyrimidine nucleotide transmembrane transporter activity Source: InterPro
  • symporter activity Source: UniProtKB-KW
  • uracil transmembrane transporter activity Source: EcoCyc

GO - Biological processi

  • uracil catabolic process Source: EcoCyc
  • uracil transport Source: EcoCyc

Keywordsi

Biological processSymport, Transport

Enzyme and pathway databases

BioCyciEcoCyc:B1006-MONOMER
MetaCyc:B1006-MONOMER

Protein family/group databases

TCDBi2.A.40.1.3 the nucleobase/ascorbate transporter (nat) or nucleobase:cation symporter-2 (ncs2) family

Names & Taxonomyi

Protein namesi
Recommended name:
Putative pyrimidine permease RutG
Gene namesi
Name:rutG
Synonyms:ycdG
Ordered Locus Names:b1006, JW5137
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG13853 rutG

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 57CytoplasmicSequence analysisAdd BLAST57
Transmembranei58 – 78HelicalSequence analysisAdd BLAST21
Topological domaini79PeriplasmicSequence analysis1
Transmembranei80 – 100HelicalSequence analysisAdd BLAST21
Topological domaini101 – 110CytoplasmicSequence analysis10
Transmembranei111 – 131HelicalSequence analysisAdd BLAST21
Topological domaini132 – 140PeriplasmicSequence analysis9
Transmembranei141 – 161HelicalSequence analysisAdd BLAST21
Topological domaini162 – 169CytoplasmicSequence analysis8
Transmembranei170 – 190HelicalSequence analysisAdd BLAST21
Topological domaini191 – 196PeriplasmicSequence analysis6
Transmembranei197 – 217HelicalSequence analysisAdd BLAST21
Topological domaini218 – 240CytoplasmicSequence analysisAdd BLAST23
Transmembranei241 – 261HelicalSequence analysisAdd BLAST21
Topological domaini262 – 284PeriplasmicSequence analysisAdd BLAST23
Transmembranei285 – 305HelicalSequence analysisAdd BLAST21
Topological domaini306 – 318CytoplasmicSequence analysisAdd BLAST13
Transmembranei319 – 339HelicalSequence analysisAdd BLAST21
Topological domaini340 – 347PeriplasmicSequence analysis8
Transmembranei348 – 368HelicalSequence analysisAdd BLAST21
Topological domaini369 – 385CytoplasmicSequence analysisAdd BLAST17
Transmembranei386 – 406HelicalSequence analysisAdd BLAST21
Transmembranei407 – 427HelicalSequence analysisAdd BLAST21
Topological domaini428 – 442CytoplasmicSequence analysisAdd BLAST15

GO - Cellular componenti

  • integral component of plasma membrane Source: EcoCyc
  • plasma membrane Source: EcoCyc

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

Pathology & Biotechi

Disruption phenotypei

Cells lacking this gene fail to grow on the nucleobase uracil as the sole nitrogen source but use the nucleoside uridine normally.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001659641 – 442Putative pyrimidine permease RutGAdd BLAST442

Proteomic databases

PaxDbiP75892
PRIDEiP75892

Expressioni

Inductioni

Up-regulated by the nitrogen regulatory protein C (NtrC also called GlnG) and repressed by RutR.2 Publications

Interactioni

Protein-protein interaction databases

BioGridi4259546, 9 interactors
STRINGi316385.ECDH10B_1078

Structurei

3D structure databases

ProteinModelPortaliP75892
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG4105C2W Bacteria
COG2233 LUCA
HOGENOMiHOG000038200
InParanoidiP75892
KOiK09016
OMAiTYWPQDQ
PhylomeDBiP75892

Family and domain databases

InterProiView protein in InterPro
IPR019918 Pyrimidine_permease_RutG_pred
IPR006042 Xan_ur_permease
IPR006043 Xant/urac/vitC
PfamiView protein in Pfam
PF00860 Xan_ur_permease, 1 hit
TIGRFAMsiTIGR00801 ncs2, 1 hit
TIGR03616 RutG, 1 hit
PROSITEiView protein in PROSITE
PS01116 XANTH_URACIL_PERMASE, 1 hit

Sequencei

Sequence statusi: Complete.

P75892-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAMFGFPHWQ LKSTSTESGV VAPDERLPFA QTAVMGVQHA VAMFGATVLM
60 70 80 90 100
PILMGLDPNL SILMSGIGTL LFFFITGGRV PSYLGSSAAF VGVVIAATGF
110 120 130 140 150
NGQGINPNIS IALGGIIACG LVYTVIGLVV MKIGTRWIER LMPPVVTGAV
160 170 180 190 200
VMAIGLNLAP IAVKSVSASA FDSWMAVMTV LCIGLVAVFT RGMIQRLLIL
210 220 230 240 250
VGLIVACLLY GVMTNVLGLG KAVDFTLVSH AAWFGLPHFS TPAFNGQAMM
260 270 280 290 300
LIAPVAVILV AENLGHLKAV AGMTGRNMDP YMGRAFVGDG LATMLSGSVG
310 320 330 340 350
GSGVTTYAEN IGVMAVTKVY STLVFVAAAV IAMLLGFSPK FGALIHTIPA
360 370 380 390 400
AVIGGASIVV FGLIAVAGAR IWVQNRVDLS QNGNLIMVAV TLVLGAGDFA
410 420 430 440
LTLGGFTLGG IGTATFGAIL LNALLSRKLV DVPPPEVVHQ EP
Length:442
Mass (Da):45,557
Last modified:December 1, 2000 - v2
Checksum:i35CC612A4E86DD67
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00096 Genomic DNA Translation: AAC74091.2
AP009048 Genomic DNA Translation: BAA35773.1
PIRiD64842
RefSeqiNP_415526.4, NC_000913.3
WP_001326838.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaiAAC74091; AAC74091; b1006
BAA35773; BAA35773; BAA35773
GeneIDi946589
KEGGiecj:JW5137
eco:b1006
PATRICifig|511145.12.peg.1044

Similar proteinsi

Entry informationi

Entry nameiRUTG_ECOLI
AccessioniPrimary (citable) accession number: P75892
Secondary accession number(s): Q9R3W5
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: December 1, 2000
Last modified: March 28, 2018
This is version 129 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health