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Protein

Protein Sxy

Gene

sxy

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Induces low levels of natural DNA uptake by inducing transcription of the competence genes (the CRP-S regulon) required for DNA transformation. Induction of the CRP-S regulon also requires Sxy-activated promoter (CRP-S), cAMP receptor protein (CRP) and cAMP (PubMed:17068078, PubMed:22532864). Induces CRP-S site-containing genes which are involved in genome maintenance and transcription or encoding transposases and toxin-antitoxin pairs (PubMed:19502395).3 Publications

Miscellaneous

Overproduction induces the expression of the competence regulon genes including ppdA, hofB and ssb, although to highly varying degrees (PubMed:25491382). Plasmid-borne expression leads to production of type IV pilin (T4P), the main subunit of the DNA uptake machinery (PubMed:19502395).2 Publications

GO - Biological processi

  • DNA import into cell involved in transformation Source: UniProtKB
  • establishment of competence for transformation Source: UniProtKB-KW
  • positive regulation of DNA binding transcription factor activity Source: EcoCyc
  • positive regulation of type IV pilus biogenesis Source: UniProtKB
  • regulation of DNA binding transcription factor activity Source: UniProtKB
  • transcription, DNA-templated Source: UniProtKB-KW

Keywordsi

Molecular functionActivator
Biological processCompetence, Transcription, Transcription regulation

Enzyme and pathway databases

BioCyciEcoCyc:G6494-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Protein Sxy
Alternative name(s):
Competence activator Sxy1 Publication
Gene namesi
Name:sxy
Synonyms:tfoX1 Publication, yccR
Ordered Locus Names:b0959, JW0942
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG13720 sxy

Pathology & Biotechi

Disruption phenotypei

Reduces both natural plasmid transformation and competitive fitness in long-term culture.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001687921 – 209Protein SxyAdd BLAST209

Proteomic databases

PaxDbiP75869
PRIDEiP75869

Expressioni

Inductioni

Not expressed by conditions that usually induce expression in other bacteria, such as amino acid starvation, antibiotics or addition of chitin. Induces its own transcription by a mechanism that requires CRP, cAMP and CRP-S site in its promoter. Negatively regulated at the post-translational level via degradation by Lon protease.1 Publication

Interactioni

Binary interactionsi

Show more details

Protein-protein interaction databases

BioGridi4263154, 7 interactors
DIPiDIP-11497N
IntActiP75869, 12 interactors
STRINGi316385.ECDH10B_1029

Structurei

3D structure databases

ProteinModelPortaliP75869
SMRiP75869
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the TfoX family.Curated

Phylogenomic databases

eggNOGiENOG4108RZ1 Bacteria
COG3070 LUCA
HOGENOMiHOG000118805
KOiK07343
OMAiDTMFALV

Family and domain databases

InterProiView protein in InterPro
IPR007077 TfoX_C
IPR007076 TfoX_N
IPR026256 TfoX_Sxy
PfamiView protein in Pfam
PF04994 TfoX_C, 1 hit
PF04993 TfoX_N, 1 hit
PIRSFiPIRSF028788 TfoX_Sxy, 1 hit

Sequencei

Sequence statusi: Complete.

P75869-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKSLSYKRIY KSQEYLATLG TIEYRSLFGS YSLTVDDTVF AMVSDGELYL
60 70 80 90 100
RACEQSAQYC VKHPPVWLTY KKCGRSVTLN YYRVDESLWR NQLKLVRLSK
110 120 130 140 150
YSLDAALKEK STRNTRERLK DLPNMSFHLE AILGEVGIKD VRALRILGAK
160 170 180 190 200
MCWLRLRQQN SLVTEKILFM LEGAIIGIHE AALPVARRQE LAEWADSLTP

KQEFPAELE
Length:209
Mass (Da):24,147
Last modified:February 1, 1997 - v1
Checksum:i588AD41143C4F2DA
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00096 Genomic DNA Translation: AAC74045.1
AP009048 Genomic DNA Translation: BAA35717.1
PIRiF64836
RefSeqiNP_415479.1, NC_000913.3
WP_000839153.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaiAAC74045; AAC74045; b0959
BAA35717; BAA35717; BAA35717
GeneIDi946504
KEGGiecj:JW0942
eco:b0959
PATRICifig|1411691.4.peg.1315

Similar proteinsi

Entry informationi

Entry nameiSXY_ECOLI
AccessioniPrimary (citable) accession number: P75869
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 15, 1998
Last sequence update: February 1, 1997
Last modified: March 28, 2018
This is version 122 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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