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Protein

Hydroxyacylglutathione hydrolase GloC

Gene

gloC

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Type II glyoxalase, isozyme of GloB, that hydrolyzes (R)-S-lactoylglutathione to (R)-lactate and glutathione. Plays a minor contribution to methylglyoxal (MG) detoxification in E.coli, compared to GloB. The two isoenzymes have additive effects and ensure maximal MG degradation.1 Publication

Catalytic activityi

S-(2-hydroxyacyl)glutathione + H2O = glutathione + a 2-hydroxy carboxylate.1 Publication
(R)-S-lactoylglutathione + H2O = (R)-lactate + glutathione.1 Publication

Cofactori

Zn2+By similarity1 PublicationNote: Binds 2 Zn2+ ions per subunit.By similarity

Enzyme regulationi

Is inhibited by Cu2+.1 Publication

Kineticsi

kcat is 0.9 sec(-1) for the hydrolysis of (R)-S-lactoylglutathione.1 Publication
  1. KM=0.5 mM for (R)-S-lactoylglutathione1 Publication

    Pathwayi: methylglyoxal degradation

    This protein is involved in step 2 of the subpathway that synthesizes (R)-lactate from methylglyoxal.1 Publication
    Proteins known to be involved in the 2 steps of the subpathway in this organism are:
    1. Lactoylglutathione lyase (gloA)
    2. Hydroxyacylglutathione hydrolase GloB (gloB), Hydroxyacylglutathione hydrolase GloC (gloC)
    This subpathway is part of the pathway methylglyoxal degradation, which is itself part of Secondary metabolite metabolism.
    View all proteins of this organism that are known to be involved in the subpathway that synthesizes (R)-lactate from methylglyoxal, the pathway methylglyoxal degradation and in Secondary metabolite metabolism.

    Sites

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Metal bindingi56Zinc 1; via tele nitrogenBy similarity1
    Metal bindingi58Zinc 1; via pros nitrogenBy similarity1
    Metal bindingi60Zinc 2By similarity1
    Metal bindingi61Zinc 2; via tele nitrogenBy similarity1
    Metal bindingi132Zinc 1; via tele nitrogenBy similarity1
    Metal bindingi151Zinc 1By similarity1
    Metal bindingi151Zinc 2By similarity1
    Metal bindingi192Zinc 2; via tele nitrogenBy similarity1

    GO - Molecular functioni

    • hydroxyacylglutathione hydrolase activity Source: EcoCyc
    • metal ion binding Source: UniProtKB-KW

    GO - Biological processi

    • methylglyoxal catabolic process to D-lactate via S-lactoyl-glutathione Source: EcoCyc
    • response to toxic substance Source: UniProtKB-KW

    Keywordsi

    Molecular functionHydrolase
    Biological processDetoxification
    LigandMetal-binding, Zinc

    Enzyme and pathway databases

    BioCyciEcoCyc:G6475-MONOMER.
    MetaCyc:G6475-MONOMER.
    UniPathwayiUPA00619; UER00676.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Hydroxyacylglutathione hydrolase GloC1 Publication (EC:3.1.2.61 Publication)
    Alternative name(s):
    Accessory type II glyoxalase1 Publication
    Glyoxalase II 21 Publication
    Short name:
    GlxII-21 Publication
    Gene namesi
    Name:gloC1 Publication
    Synonyms:ycbLImported
    Ordered Locus Names:b0927, JW0910
    OrganismiEscherichia coli (strain K12)
    Taxonomic identifieri83333 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
    Proteomesi
    • UP000000318 Componenti: Chromosome
    • UP000000625 Componenti: Chromosome

    Organism-specific databases

    EcoGeneiEG13704. ycbL.

    Subcellular locationi

    GO - Cellular componenti

    • cytosol Source: EcoCyc

    Pathology & Biotechi

    Disruption phenotypei

    Cells lacking this gene show decreased methylglyoxal tolerance. A double deletion mutant lacking both gloC and gloB exhibits almost no resistance to exogenously supplied methylglyoxal, and is unable to grow at MG concentrations as low as 0.1 mM.1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    ChainiPRO_00001923571 – 215Hydroxyacylglutathione hydrolase GloCAdd BLAST215

    Proteomic databases

    EPDiP75849.
    PaxDbiP75849.
    PRIDEiP75849.

    Interactioni

    Protein-protein interaction databases

    BioGridi4261607. 16 interactors.
    DIPiDIP-11480N.
    IntActiP75849. 4 interactors.
    MINTiMINT-1282138.
    STRINGi316385.ECDH10B_0997.

    Structurei

    3D structure databases

    ProteinModelPortaliP75849.
    SMRiP75849.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Phylogenomic databases

    eggNOGiENOG4105C5X. Bacteria.
    COG0491. LUCA.
    HOGENOMiHOG000058039.
    InParanoidiP75849.
    PhylomeDBiP75849.

    Family and domain databases

    Gene3Di3.60.15.10. 1 hit.
    InterProiView protein in InterPro
    IPR001279. Metallo-B-lactamas.
    PfamiView protein in Pfam
    PF00753. Lactamase_B. 1 hit.
    SMARTiView protein in SMART
    SM00849. Lactamase_B. 1 hit.
    SUPFAMiSSF56281. SSF56281. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    P75849-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MNYRIIPVTA FSQNCSLIWC EQTRLAALVD PGGDAEKIKQ EVDDSGLTLM
    60 70 80 90 100
    QILLTHGHLD HVGAAAELAQ HYGVPVFGPE KEDEFWLQGL PAQSRMFGLE
    110 120 130 140 150
    ECQPLTPDRW LNEGDTISIG NVTLQVLHCP GHTPGHVVFF DDRAKLLISG
    160 170 180 190 200
    DVIFKGGVGR SDFPRGDHNQ LISSIKDKLL PLGDDVIFIP GHGPLSTLGY
    210
    ERLHNPFLQD EMPVW
    Length:215
    Mass (Da):23,784
    Last modified:February 1, 1997 - v1
    Checksum:iC4233FF08308B18D
    GO

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    U00096 Genomic DNA. Translation: AAC74013.1.
    AP009048 Genomic DNA. Translation: BAA35673.1.
    PIRiF64832.
    RefSeqiNP_415447.1. NC_000913.3.
    WP_001109486.1. NZ_LN832404.1.

    Genome annotation databases

    EnsemblBacteriaiAAC74013; AAC74013; b0927.
    BAA35673; BAA35673; BAA35673.
    GeneIDi945551.
    KEGGiecj:JW0910.
    eco:b0927.
    PATRICifig|1411691.4.peg.1349.

    Similar proteinsi

    Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
    100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
    90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
    50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

    Entry informationi

    Entry nameiGLO22_ECOLI
    AccessioniPrimary (citable) accession number: P75849
    Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1997
    Last sequence update: February 1, 1997
    Last modified: July 5, 2017
    This is version 125 of the entry and version 1 of the sequence. See complete history.
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Escherichia coli
      Escherichia coli (strain K12): entries and cross-references to EcoGene
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. SIMILARITY comments
      Index of protein domains and families