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P75835 (YCAM_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 97. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Inner membrane transporter YcaM
Gene names
Name:ycaM
Ordered Locus Names:b0899, JW5119
OrganismEscherichia coli (strain K12) [Reference proteome] [HAMAP]
Taxonomic identifier83333 [NCBI]
Taxonomic lineageBacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia ›

Protein attributes

Sequence length476 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Subcellular location

Cell inner membrane; Multi-pass membrane protein.

Sequence similarities

Belongs to the amino acid-polyamine-organocation (APC) superfamily.

Ontologies

Keywords
   Biological processTransport
   Cellular componentCell inner membrane
Cell membrane
Membrane
   DomainTransmembrane
Transmembrane helix
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Cellular_componentintegral to membrane

Inferred from electronic annotation. Source: UniProtKB-KW

plasma membrane

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionamino acid transmembrane transporter activity

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 476476Inner membrane transporter YcaM
PRO_0000213044

Regions

Topological domain1 – 99Cytoplasmic Potential
Transmembrane10 – 3021Helical; Potential
Topological domain31 – 388Periplasmic Potential
Transmembrane39 – 5921Helical; Potential
Topological domain60 – 8021Cytoplasmic Potential
Transmembrane81 – 10121Helical; Potential
Topological domain102 – 12524Periplasmic Potential
Transmembrane126 – 14621Helical; Potential
Topological domain147 – 1548Cytoplasmic Potential
Transmembrane155 – 17521Helical; Potential
Topological domain176 – 19520Periplasmic Potential
Transmembrane196 – 21621Helical; Potential
Topological domain217 – 24024Cytoplasmic Potential
Transmembrane241 – 26121Helical; Potential
Topological domain262 – 29130Periplasmic Potential
Transmembrane292 – 31221Helical; Potential
Topological domain313 – 34331Cytoplasmic Potential
Transmembrane344 – 36421Helical; Potential
Topological domain365 – 37511Periplasmic Potential
Transmembrane376 – 39621Helical; Potential
Topological domain397 – 41418Cytoplasmic Potential
Transmembrane415 – 43521Helical; Potential
Topological domain436 – 44813Periplasmic Potential
Transmembrane449 – 46921Helical; Potential
Topological domain470 – 4767Cytoplasmic Potential

Sequences

Sequence LengthMass (Da)Tools
P75835 [UniParc].

Last modified May 30, 2000. Version 2.
Checksum: EEE28BE119576B21

FASTA47652,496
        10         20         30         40         50         60 
MAGNVQEKQL RWYNIALMSF ITVWGFGNVV NNYANQGLVV VFSWVFIFAL YFTPYALIVG 

        70         80         90        100        110        120 
QLGSTFKDGK GGVSTWIKHT MGPGLAYLAA WTYWVVHIPY LAQKPQAILI ALGWAMKGDG 

       130        140        150        160        170        180 
SLIKEYSVVA LQGLTLVLFI FFMWVASRGM KSLKIVGSVA GIAMFVMSLL YVAMAVTAPA 

       190        200        210        220        230        240 
ITEVHIATTN ITWETFIPHI DFTYITTISM LVFAVGGAEK ISPYVNQTRN PGKEFPKGML 

       250        260        270        280        290        300 
CLAVMVAVCA ILGSLAMGMM FDSRNIPDDL MTNGQYYAFQ KLGEYYNMGN TLMVIYAIAN 

       310        320        330        340        350        360 
TLGQVAALVF SIDAPLKVLL GDADSKYIPA SLCRTNASGT PVNGYFLTLV LVAILIMLPT 

       370        380        390        400        410        420 
LGIGDMNNLY KWLLNLNSVV MPLRYLWVFV AFIAVVRLAQ KYKPEYVFIR NKPLAMTVGI 

       430        440        450        460        470 
WCFAFTAFAC LTGIFPKMEA FTAEWTFQLA LNVATPFVLV GLGLIFPLLA RKANSK

« Hide

References

[1]"A 718-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 12.7-28.0 min region on the linkage map."
Oshima T., Aiba H., Baba T., Fujita K., Hayashi K., Honjo A., Ikemoto K., Inada T., Itoh T., Kajihara M., Kanai K., Kashimoto K., Kimura S., Kitagawa M., Makino K., Masuda S., Miki T., Mizobuchi K. expand/collapse author list , Mori H., Motomura K., Nakamura Y., Nashimoto H., Nishio Y., Saito N., Sampei G., Seki Y., Tagami H., Takemoto K., Wada C., Yamamoto Y., Yano M., Horiuchi T.
DNA Res. 3:137-155(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[2]"The complete genome sequence of Escherichia coli K-12."
Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y.
Science 277:1453-1474(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / MG1655 / ATCC 47076.
[3]"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[4]"Global topology analysis of the Escherichia coli inner membrane proteome."
Daley D.O., Rapp M., Granseth E., Melen K., Drew D., von Heijne G.
Science 308:1321-1323(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: TOPOLOGY [LARGE SCALE ANALYSIS].
Strain: K12 / MG1655 / ATCC 47076.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		U00096 Genomic DNA. Translation: AAC73985.2.
AP009048 Genomic DNA. Translation: BAA35634.2.
PIRB64829.
RefSeqNP_415419.2. NC_000913.2.
YP_489171.1. NC_007779.1.

3D structure databases

ProteinModelPortalP75835.
SMRP75835. Positions 33-436.
ModBaseSearch...

Protein-protein interaction databases

DIPDIP-11468N.
STRING511145.b0899.

Protein family/group databases

TCDB2.A.3.7.2. amino acid-polyamine-organocation (APC) family.

Proteomic databases

PRIDEP75835.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAC73985; AAC73985; b0899.
BAA35634; BAA35634; BAA35634.
GeneID12932119.
945518.
KEGGecj:Y75_p0871.
eco:b0899.
PATRIC32117011. VBIEscCol129921_0930.

Organism-specific databases

EchoBASEEB3461.
EcoGeneEG13697. ycaM.

Phylogenomic databases

eggNOGCOG0531.
HOGENOMHOG000057650.
OMAVMPMRYL.
ProtClustDBCLSK876836.

Enzyme and pathway databases

BioCycEcoCyc:YCAM.
ECOL316407:JW5119-MONOMER.

Gene expression databases

GenevestigatorP75835.

Family and domain databases

InterProIPR002293. AA/rel_permease1.
[Graphical view]
PANTHERPTHR11785. PTHR11785. 1 hit.
PIRSFPIRSF006060. AA_transporter. 1 hit.
ProtoNetSearch...

Entry information

Entry nameYCAM_ECOLI
AccessionPrimary (citable) accession number: P75835
Secondary accession number(s): Q9R7Q7
Entry history
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: May 30, 2000
Last modified: May 1, 2013
This is version 97 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

Escherichia coli

Escherichia coli (strain K12): entries and cross-references to EcoGene

SIMILARITY comments

Index of protein domains and families

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