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Protein

Macrolide export ATP-binding/permease protein MacB

Gene

macB

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Part of the tripartite efflux system MacAB-TolC. MacB is a non-canonical ABC transporter that contains transmembrane domains (TMD), which form a pore in the inner membrane, and an ATP-binding domain (NBD), which is responsible for energy generation. When overexpressed, the system confers resistance against macrolides composed of 14- and 15-membered lactones but no or weak resistance against 16-membered ones. In addition, the system could also transport R-LPS or a similar glycolipid.4 Publications

Enzyme regulationi

ATPase activity is stimulated by interaction with MacA and inhibited by vanadate.3 Publications

Kineticsi

kcat is 0.78 sec(-1) for ATP in the presence of MacA. kcat is 0.10 sec(-1) for ATP in the absence of MacA.
  1. KM=0.38 mM for ATP (in the presence of MacA)1 Publication
  2. KM=2.30 mM for ATP (in the absence of MacA)1 Publication

    Regions

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Nucleotide bindingi41 – 48ATPUniRule annotation8

    GO - Molecular functioni

    • antibiotic transmembrane transporter activity Source: EcoCyc
    • ATP binding Source: UniProtKB-KW
    • identical protein binding Source: IntAct
    • xenobiotic transmembrane transporting ATPase activity Source: EcoCyc

    GO - Biological processi

    • drug transmembrane transport Source: EcoCyc
    • response to antibiotic Source: EcoCyc

    Keywordsi

    Molecular functionHydrolase
    Biological processAntibiotic resistance, Transport
    LigandATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciEcoCyc:MACB.

    Protein family/group databases

    TCDBi3.A.1.122.1. the atp-binding cassette (abc) superfamily.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Macrolide export ATP-binding/permease protein MacBUniRule annotation (EC:3.6.3.-UniRule annotation)
    Gene namesi
    Name:macBUniRule annotation
    Synonyms:ybjZ
    Ordered Locus Names:b0879, JW0863
    OrganismiEscherichia coli (strain K12)
    Taxonomic identifieri83333 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
    Proteomesi
    • UP000000318 Componenti: Chromosome
    • UP000000625 Componenti: Chromosome

    Organism-specific databases

    EcoGeneiEG13695. macB.

    Subcellular locationi

    Topology

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Topological domaini1 – 272CytoplasmicSequence analysisAdd BLAST272
    Transmembranei273 – 293HelicalUniRule annotationAdd BLAST21
    Topological domaini294 – 522PeriplasmicSequence analysisAdd BLAST229
    Transmembranei523 – 543HelicalUniRule annotationAdd BLAST21
    Topological domaini544 – 575CytoplasmicSequence analysisAdd BLAST32
    Transmembranei576 – 596HelicalUniRule annotationAdd BLAST21
    Topological domaini597 – 610PeriplasmicSequence analysisAdd BLAST14
    Transmembranei611 – 631HelicalUniRule annotationAdd BLAST21
    Topological domaini632 – 648CytoplasmicSequence analysisAdd BLAST17

    GO - Cellular componenti

    • integral component of membrane Source: EcoliWiki
    • integral component of plasma membrane Source: EcoCyc
    • plasma membrane Source: EcoCyc

    Keywords - Cellular componenti

    Cell inner membrane, Cell membrane, Membrane

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Mutagenesisi47K → L: Lack of activity. 1 Publication1
    Mutagenesisi169D → N: Lack of activity. 1 Publication1

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    ChainiPRO_00000924741 – 648Macrolide export ATP-binding/permease protein MacBAdd BLAST648

    Proteomic databases

    PaxDbiP75831.
    PRIDEiP75831.

    Interactioni

    Subunit structurei

    Homodimer. Part of the tripartite efflux system MacAB-TolC, which is composed of an inner membrane transporter, MacB, a periplasmic membrane fusion protein, MacA, and an outer membrane component, TolC. The complex forms a large protein conduit and can translocate molecules across both the inner and outer membranes. Interacts with MacA.UniRule annotation3 Publications

    Binary interactionsi

    Show more details

    GO - Molecular functioni

    • identical protein binding Source: IntAct

    Protein-protein interaction databases

    BioGridi4261698. 110 interactors.
    IntActiP75831. 3 interactors.
    STRINGi316385.ECDH10B_0949.

    Structurei

    Secondary structure

    1648
    Legend: HelixTurnBeta strandPDB Structure known for this area
    Show more details
    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Beta strandi4 – 9Combined sources6
    Beta strandi14 – 16Combined sources3
    Beta strandi19 – 23Combined sources5
    Helixi47 – 54Combined sources8
    Beta strandi61 – 67Combined sources7
    Turni72 – 74Combined sources3
    Helixi77 – 86Combined sources10
    Beta strandi99 – 101Combined sources3
    Helixi103 – 112Combined sources10
    Helixi118 – 132Combined sources15
    Helixi136 – 139Combined sources4
    Helixi142 – 144Combined sources3
    Helixi147 – 159Combined sources13
    Turni160 – 162Combined sources3
    Beta strandi164 – 169Combined sources6
    Turni171 – 174Combined sources4
    Helixi177 – 192Combined sources16
    Beta strandi196 – 200Combined sources5
    Helixi204 – 207Combined sources4
    Beta strandi210 – 212Combined sources3
    Beta strandi215 – 217Combined sources3
    Beta strandi220 – 223Combined sources4
    Helixi248 – 267Combined sources20
    Helixi269 – 305Combined sources37
    Beta strandi317 – 319Combined sources3
    Helixi324 – 327Combined sources4
    Helixi333 – 335Combined sources3
    Helixi336 – 341Combined sources6
    Beta strandi345 – 353Combined sources9
    Beta strandi356 – 359Combined sources4
    Beta strandi364 – 367Combined sources4
    Beta strandi370 – 372Combined sources3
    Helixi376 – 380Combined sources5
    Beta strandi384 – 387Combined sources4
    Helixi392 – 395Combined sources4
    Turni396 – 398Combined sources3
    Helixi406 – 412Combined sources7
    Beta strandi423 – 426Combined sources4
    Beta strandi429 – 432Combined sources4
    Helixi444 – 446Combined sources3
    Beta strandi452 – 455Combined sources4
    Helixi456 – 459Combined sources4
    Turni460 – 464Combined sources5
    Beta strandi470 – 476Combined sources7
    Helixi486 – 493Combined sources8
    Beta strandi496 – 500Combined sources5
    Helixi508 – 550Combined sources43
    Helixi552 – 560Combined sources9
    Helixi565 – 600Combined sources36
    Helixi611 – 638Combined sources28
    Helixi641 – 646Combined sources6

    3D structure databases

    Select the link destinations:
    PDBei
    RCSB PDBi
    PDBji
    Links Updated
    PDB entryMethodResolution (Å)ChainPositionsPDBsum
    5LJ8X-ray1.95A/B309-508[»]
    5LJ9X-ray2.30A/B/C1-223[»]
    5LJAX-ray2.40A1-223[»]
    5NIKelectron microscopy3.30J/K1-648[»]
    5NILelectron microscopy5.30J/K1-648[»]
    ProteinModelPortaliP75831.
    SMRiP75831.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Domaini5 – 243ABC transporterUniRule annotationAdd BLAST239

    Sequence similaritiesi

    Belongs to the ABC transporter superfamily. Macrolide exporter (TC 3.A.1.122) family. [View classification]UniRule annotation

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiENOG4105D6C. Bacteria.
    COG0577. LUCA.
    COG1136. LUCA.
    HOGENOMiHOG000208217.
    InParanoidiP75831.
    KOiK05685.
    PhylomeDBiP75831.

    Family and domain databases

    InterProiView protein in InterPro
    IPR003593. AAA+_ATPase.
    IPR003838. ABC_permease_dom.
    IPR003439. ABC_transporter-like.
    IPR017871. ABC_transporter_CS.
    IPR017911. ABC_transptr_macrolide_ATP-bd.
    IPR025857. MacB_PCD.
    IPR027417. P-loop_NTPase.
    PfamiView protein in Pfam
    PF00005. ABC_tran. 1 hit.
    PF02687. FtsX. 1 hit.
    PF12704. MacB_PCD. 1 hit.
    SMARTiView protein in SMART
    SM00382. AAA. 1 hit.
    SUPFAMiSSF52540. SSF52540. 1 hit.
    PROSITEiView protein in PROSITE
    PS00211. ABC_TRANSPORTER_1. 1 hit.
    PS50893. ABC_TRANSPORTER_2. 1 hit.
    PS51267. MACB. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    P75831-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MTPLLELKDI RRSYPAGDEQ VEVLKGISLD IYAGEMVAIV GASGSGKSTL
    60 70 80 90 100
    MNILGCLDKA TSGTYRVAGQ DVATLDADAL AQLRREHFGF IFQRYHLLSH
    110 120 130 140 150
    LTAEQNVEVP AVYAGLERKQ RLLRAQELLQ RLGLEDRTEY YPAQLSGGQQ
    160 170 180 190 200
    QRVSIARALM NGGQVILADE PTGALDSHSG EEVMAILHQL RDRGHTVIIV
    210 220 230 240 250
    THDPQVAAQA ERVIEIRDGE IVRNPPAIEK VNVTGGTEPV VNTVSGWRQF
    260 270 280 290 300
    VSGFNEALTM AWRALAANKM RTLLTMLGII IGIASVVSIV VVGDAAKQMV
    310 320 330 340 350
    LADIRSIGTN TIDVYPGKDF GDDDPQYQQA LKYDDLIAIQ KQPWVASATP
    360 370 380 390 400
    AVSQNLRLRY NNVDVAASAN GVSGDYFNVY GMTFSEGNTF NQEQLNGRAQ
    410 420 430 440 450
    VVVLDSNTRR QLFPHKADVV GEVILVGNMP ARVIGVAEEK QSMFGSSKVL
    460 470 480 490 500
    RVWLPYSTMS GRVMGQSWLN SITVRVKEGF DSAEAEQQLT RLLSLRHGKK
    510 520 530 540 550
    DFFTWNMDGV LKTVEKTTRT LQLFLTLVAV ISLVVGGIGV MNIMLVSVTE
    560 570 580 590 600
    RTREIGIRMA VGARASDVLQ QFLIEAVLVC LVGGALGITL SLLIAFTLQL
    610 620 630 640
    FLPGWEIGFS PLALLLAFLC STVTGILFGW LPARNAARLD PVDALARE
    Length:648
    Mass (Da):70,702
    Last modified:February 1, 1997 - v1
    Checksum:i157B7D6E1ED17346
    GO

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AB071146 Genomic DNA. Translation: BAB64542.1.
    U00096 Genomic DNA. Translation: AAC73966.1.
    AP009048 Genomic DNA. Translation: BAA35598.1.
    PIRiG64826.
    RefSeqiNP_415400.1. NC_000913.3.
    WP_000188144.1. NZ_LN832404.1.

    Genome annotation databases

    EnsemblBacteriaiAAC73966; AAC73966; b0879.
    BAA35598; BAA35598; BAA35598.
    GeneIDi945164.
    KEGGiecj:JW0863.
    eco:b0879.
    PATRICifig|1411691.4.peg.1398.

    Similar proteinsi

    Entry informationi

    Entry nameiMACB_ECOLI
    AccessioniPrimary (citable) accession number: P75831
    Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1997
    Last sequence update: February 1, 1997
    Last modified: January 31, 2018
    This is version 147 of the entry and version 1 of the sequence. See complete history.
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. Escherichia coli
      Escherichia coli (strain K12): entries and cross-references to EcoGene
    2. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    3. SIMILARITY comments
      Index of protein domains and families