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Protein

Macrolide export ATP-binding/permease protein MacB

Gene

macB

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Part of the tripartite efflux system MacAB-TolC. MacB is a non-canonical ABC transporter that contains transmembrane domains (TMD), which form a pore in the inner membrane, and an ATP-binding domain (NBD), which is responsible for energy generation. When overexpressed, the system confers resistance against macrolides composed of 14- and 15-membered lactones but no or weak resistance against 16-membered ones. In addition, the system could also transport R-LPS or a similar glycolipid.4 Publications

Enzyme regulationi

ATPase activity is stimulated by interaction with MacA and inhibited by vanadate.3 Publications

Kineticsi

kcat is 0.78 sec(-1) for ATP in the presence of MacA. kcat is 0.10 sec(-1) for ATP in the absence of MacA.

  1. KM=0.38 mM for ATP (in the presence of MacA)1 Publication
  2. KM=2.30 mM for ATP (in the absence of MacA)1 Publication

    Regions

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Nucleotide bindingi41 – 48ATPUniRule annotation8

    GO - Molecular functioni

    • antibiotic transporter activity Source: EcoCyc
    • ATP binding Source: UniProtKB-HAMAP
    • identical protein binding Source: IntAct
    • xenobiotic-transporting ATPase activity Source: EcoCyc

    GO - Biological processi

    • drug transmembrane transport Source: EcoCyc
    • response to antibiotic Source: EcoCyc
    Complete GO annotation...

    Keywords - Molecular functioni

    Hydrolase

    Keywords - Biological processi

    Antibiotic resistance, Transport

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciEcoCyc:MACB.
    ECOL316407:JW0863-MONOMER.

    Protein family/group databases

    TCDBi3.A.1.122.1. the atp-binding cassette (abc) superfamily.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Macrolide export ATP-binding/permease protein MacBUniRule annotation (EC:3.6.3.-UniRule annotation)
    Gene namesi
    Name:macBUniRule annotation
    Synonyms:ybjZ
    Ordered Locus Names:b0879, JW0863
    OrganismiEscherichia coli (strain K12)
    Taxonomic identifieri83333 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
    Proteomesi
    • UP000000318 Componenti: Chromosome
    • UP000000625 Componenti: Chromosome

    Organism-specific databases

    EcoGeneiEG13695. macB.

    Subcellular locationi

    • Cell inner membrane UniRule annotation2 Publications; Multi-pass membrane protein UniRule annotation2 Publications

    Topology

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Topological domaini1 – 272CytoplasmicSequence analysisAdd BLAST272
    Transmembranei273 – 293HelicalUniRule annotationAdd BLAST21
    Topological domaini294 – 522PeriplasmicSequence analysisAdd BLAST229
    Transmembranei523 – 543HelicalUniRule annotationAdd BLAST21
    Topological domaini544 – 575CytoplasmicSequence analysisAdd BLAST32
    Transmembranei576 – 596HelicalUniRule annotationAdd BLAST21
    Topological domaini597 – 610PeriplasmicSequence analysisAdd BLAST14
    Transmembranei611 – 631HelicalUniRule annotationAdd BLAST21
    Topological domaini632 – 648CytoplasmicSequence analysisAdd BLAST17

    GO - Cellular componenti

    • ATP-binding cassette (ABC) transporter complex Source: UniProtKB-HAMAP
    • integral component of membrane Source: EcoliWiki
    • integral component of plasma membrane Source: EcoCyc
    • plasma membrane Source: EcoCyc
    Complete GO annotation...

    Keywords - Cellular componenti

    Cell inner membrane, Cell membrane, Membrane

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Mutagenesisi47K → L: Lack of activity. 1 Publication1
    Mutagenesisi169D → N: Lack of activity. 1 Publication1

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    ChainiPRO_00000924741 – 648Macrolide export ATP-binding/permease protein MacBAdd BLAST648

    Proteomic databases

    PaxDbiP75831.
    PRIDEiP75831.

    Interactioni

    Subunit structurei

    Homodimer. Part of the tripartite efflux system MacAB-TolC, which is composed of an inner membrane transporter, MacB, a periplasmic membrane fusion protein, MacA, and an outer membrane component, TolC. The complex forms a large protein conduit and can translocate molecules across both the inner and outer membranes. Interacts with MacA.UniRule annotation3 Publications

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    itself4EBI-1125580,EBI-1125580
    macAP758302EBI-1125580,EBI-551961

    GO - Molecular functioni

    • identical protein binding Source: IntAct

    Protein-protein interaction databases

    BioGridi4261698. 110 interactors.
    IntActiP75831. 3 interactors.
    STRINGi511145.b0879.

    Structurei

    3D structure databases

    ProteinModelPortaliP75831.
    SMRiP75831.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Domaini5 – 243ABC transporterUniRule annotationAdd BLAST239

    Sequence similaritiesi

    Belongs to the ABC transporter superfamily. Macrolide exporter (TC 3.A.1.122) family. [View classification]UniRule annotation
    Contains 1 ABC transporter domain.UniRule annotation

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiENOG4105D6C. Bacteria.
    COG0577. LUCA.
    COG1136. LUCA.
    HOGENOMiHOG000208217.
    InParanoidiP75831.
    KOiK05685.
    OMAiMAWRAMA.
    PhylomeDBiP75831.

    Family and domain databases

    Gene3Di3.40.50.300. 1 hit.
    InterProiIPR003593. AAA+_ATPase.
    IPR003838. ABC_permease_dom.
    IPR003439. ABC_transporter-like.
    IPR017871. ABC_transporter_CS.
    IPR017911. ABC_transptr_macrolide_ATP-bd.
    IPR025857. MacB_PCD.
    IPR027417. P-loop_NTPase.
    [Graphical view]
    PfamiPF00005. ABC_tran. 1 hit.
    PF02687. FtsX. 1 hit.
    PF12704. MacB_PCD. 1 hit.
    [Graphical view]
    SMARTiSM00382. AAA. 1 hit.
    [Graphical view]
    SUPFAMiSSF52540. SSF52540. 1 hit.
    PROSITEiPS00211. ABC_TRANSPORTER_1. 1 hit.
    PS50893. ABC_TRANSPORTER_2. 1 hit.
    PS51267. MACB. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P75831-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MTPLLELKDI RRSYPAGDEQ VEVLKGISLD IYAGEMVAIV GASGSGKSTL
    60 70 80 90 100
    MNILGCLDKA TSGTYRVAGQ DVATLDADAL AQLRREHFGF IFQRYHLLSH
    110 120 130 140 150
    LTAEQNVEVP AVYAGLERKQ RLLRAQELLQ RLGLEDRTEY YPAQLSGGQQ
    160 170 180 190 200
    QRVSIARALM NGGQVILADE PTGALDSHSG EEVMAILHQL RDRGHTVIIV
    210 220 230 240 250
    THDPQVAAQA ERVIEIRDGE IVRNPPAIEK VNVTGGTEPV VNTVSGWRQF
    260 270 280 290 300
    VSGFNEALTM AWRALAANKM RTLLTMLGII IGIASVVSIV VVGDAAKQMV
    310 320 330 340 350
    LADIRSIGTN TIDVYPGKDF GDDDPQYQQA LKYDDLIAIQ KQPWVASATP
    360 370 380 390 400
    AVSQNLRLRY NNVDVAASAN GVSGDYFNVY GMTFSEGNTF NQEQLNGRAQ
    410 420 430 440 450
    VVVLDSNTRR QLFPHKADVV GEVILVGNMP ARVIGVAEEK QSMFGSSKVL
    460 470 480 490 500
    RVWLPYSTMS GRVMGQSWLN SITVRVKEGF DSAEAEQQLT RLLSLRHGKK
    510 520 530 540 550
    DFFTWNMDGV LKTVEKTTRT LQLFLTLVAV ISLVVGGIGV MNIMLVSVTE
    560 570 580 590 600
    RTREIGIRMA VGARASDVLQ QFLIEAVLVC LVGGALGITL SLLIAFTLQL
    610 620 630 640
    FLPGWEIGFS PLALLLAFLC STVTGILFGW LPARNAARLD PVDALARE
    Length:648
    Mass (Da):70,702
    Last modified:February 1, 1997 - v1
    Checksum:i157B7D6E1ED17346
    GO

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AB071146 Genomic DNA. Translation: BAB64542.1.
    U00096 Genomic DNA. Translation: AAC73966.1.
    AP009048 Genomic DNA. Translation: BAA35598.1.
    PIRiG64826.
    RefSeqiNP_415400.1. NC_000913.3.
    WP_000188144.1. NZ_LN832404.1.

    Genome annotation databases

    EnsemblBacteriaiAAC73966; AAC73966; b0879.
    BAA35598; BAA35598; BAA35598.
    GeneIDi945164.
    KEGGiecj:JW0863.
    eco:b0879.
    PATRICi32116967. VBIEscCol129921_0909.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AB071146 Genomic DNA. Translation: BAB64542.1.
    U00096 Genomic DNA. Translation: AAC73966.1.
    AP009048 Genomic DNA. Translation: BAA35598.1.
    PIRiG64826.
    RefSeqiNP_415400.1. NC_000913.3.
    WP_000188144.1. NZ_LN832404.1.

    3D structure databases

    ProteinModelPortaliP75831.
    SMRiP75831.
    ModBaseiSearch...
    MobiDBiSearch...

    Protein-protein interaction databases

    BioGridi4261698. 110 interactors.
    IntActiP75831. 3 interactors.
    STRINGi511145.b0879.

    Protein family/group databases

    TCDBi3.A.1.122.1. the atp-binding cassette (abc) superfamily.

    Proteomic databases

    PaxDbiP75831.
    PRIDEiP75831.

    Protocols and materials databases

    Structural Biology KnowledgebaseSearch...

    Genome annotation databases

    EnsemblBacteriaiAAC73966; AAC73966; b0879.
    BAA35598; BAA35598; BAA35598.
    GeneIDi945164.
    KEGGiecj:JW0863.
    eco:b0879.
    PATRICi32116967. VBIEscCol129921_0909.

    Organism-specific databases

    EchoBASEiEB3459.
    EcoGeneiEG13695. macB.

    Phylogenomic databases

    eggNOGiENOG4105D6C. Bacteria.
    COG0577. LUCA.
    COG1136. LUCA.
    HOGENOMiHOG000208217.
    InParanoidiP75831.
    KOiK05685.
    OMAiMAWRAMA.
    PhylomeDBiP75831.

    Enzyme and pathway databases

    BioCyciEcoCyc:MACB.
    ECOL316407:JW0863-MONOMER.

    Miscellaneous databases

    PROiP75831.

    Family and domain databases

    Gene3Di3.40.50.300. 1 hit.
    InterProiIPR003593. AAA+_ATPase.
    IPR003838. ABC_permease_dom.
    IPR003439. ABC_transporter-like.
    IPR017871. ABC_transporter_CS.
    IPR017911. ABC_transptr_macrolide_ATP-bd.
    IPR025857. MacB_PCD.
    IPR027417. P-loop_NTPase.
    [Graphical view]
    PfamiPF00005. ABC_tran. 1 hit.
    PF02687. FtsX. 1 hit.
    PF12704. MacB_PCD. 1 hit.
    [Graphical view]
    SMARTiSM00382. AAA. 1 hit.
    [Graphical view]
    SUPFAMiSSF52540. SSF52540. 1 hit.
    PROSITEiPS00211. ABC_TRANSPORTER_1. 1 hit.
    PS50893. ABC_TRANSPORTER_2. 1 hit.
    PS51267. MACB. 1 hit.
    [Graphical view]
    ProtoNetiSearch...

    Entry informationi

    Entry nameiMACB_ECOLI
    AccessioniPrimary (citable) accession number: P75831
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1997
    Last sequence update: February 1, 1997
    Last modified: November 2, 2016
    This is version 139 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Escherichia coli
      Escherichia coli (strain K12): entries and cross-references to EcoGene
    2. SIMILARITY comments
      Index of protein domains and families

    Similar proteinsi

    Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
    100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
    90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
    50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.