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Protein

Hydroxylamine reductase

Gene

hcp

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the reduction of hydroxylamine to form NH3 and H2O. Is also able to reduce hydroxylamine analogs such as methylhydroxylamine and hydroxyquinone. Might have a role as a scavenger of potentially toxic by-products of nitrate metabolism.UniRule annotation2 Publications

Catalytic activityi

NH3 + H2O + acceptor = hydroxylamine + reduced acceptor.UniRule annotation1 Publication

Cofactori

Protein has several cofactor binding sites:

Enzyme regulationi

Inhibited by oxygen. Activated by cyanide except in the prolonged presence of excess cyanide, where the enzyme is inactivated.1 Publication

Kineticsi

  1. KM=2.5 mM for hydroxylamine (at pH 9)1 Publication
  1. Vmax=92 µmol/min/mg enzyme (at pH 7.5)1 Publication
  2. Vmax=458 µmol/min/mg enzyme (at pH 9)1 Publication

pH dependencei

Optimum pH is 9.0.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi3Iron-sulfur (2Fe-2S)UniRule annotation1
Metal bindingi6Iron-sulfur (2Fe-2S)UniRule annotation1
Metal bindingi18Iron-sulfur (2Fe-2S)UniRule annotation1
Metal bindingi25Iron-sulfur (2Fe-2S)UniRule annotation1
Metal bindingi249Iron-oxo-sulfur (4Fe-2O-2S); via tele nitrogenUniRule annotation1
Metal bindingi273Iron-oxo-sulfur (4Fe-2O-2S)UniRule annotation1
Metal bindingi317Iron-oxo-sulfur (4Fe-2O-2S)UniRule annotation1
Metal bindingi405Iron-oxo-sulfur (4Fe-2O-2S); via persulfide groupUniRule annotation1
Metal bindingi433Iron-oxo-sulfur (4Fe-2O-2S)UniRule annotation1
Metal bindingi458Iron-oxo-sulfur (4Fe-2O-2S)UniRule annotation1
Metal bindingi492Iron-oxo-sulfur (4Fe-2O-2S)UniRule annotation1
Metal bindingi494Iron-oxo-sulfur (4Fe-2O-2S)UniRule annotation1

GO - Molecular functioni

  • 2 iron, 2 sulfur cluster binding Source: UniProtKB-KW
  • hydroxylamine reductase activity Source: EcoCyc
  • metal ion binding Source: UniProtKB-KW
  • oxidoreductase activity Source: EcoliWiki
  • peroxidase activity Source: EcoliWiki

GO - Biological processi

  • nitric oxide catabolic process Source: EcoCyc
  • nitrogen compound metabolic process Source: EcoCyc
  • oxidation-reduction process Source: EcoliWiki
  • response to hydrogen peroxide Source: EcoliWiki

Keywordsi

Molecular functionOxidoreductase
Ligand2Fe-2S, Iron, Iron-sulfur, Metal-binding

Enzyme and pathway databases

BioCyciEcoCyc:G6457-MONOMER.
MetaCyc:G6457-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Hydroxylamine reductase1 Publication (EC:1.7.99.11 Publication)
Alternative name(s):
Hybrid-cluster proteinUniRule annotation
Short name:
HCPUniRule annotation
Prismane protein1 Publication
Gene namesi
Name:hcpUniRule annotation
Synonyms:ybjW
Ordered Locus Names:b0873, JW0857
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG13692. hcp.

Subcellular locationi

P75825:
  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001516741 – 550Hydroxylamine reductaseAdd BLAST550

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei405Cysteine persulfideUniRule annotation1

Proteomic databases

PaxDbiP75825.
PRIDEiP75825.

Expressioni

Inductioni

By Fnr, NarL and NarP under anaerobic conditions in the presence of either nitrate or nitrite.1 Publication

Interactioni

Protein-protein interaction databases

BioGridi4262100. 10 interactors.
DIPiDIP-9870N.
IntActiP75825. 5 interactors.
STRINGi316385.ECDH10B_0943.

Structurei

3D structure databases

ProteinModelPortaliP75825.
SMRiP75825.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the HCP family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105EJ7. Bacteria.
COG1151. LUCA.
HOGENOMiHOG000007176.
InParanoidiP75825.
KOiK05601.
PhylomeDBiP75825.

Family and domain databases

CDDicd01914. HCP. 1 hit.
Gene3Di1.20.1270.20. 2 hits.
3.40.50.2030. 2 hits.
HAMAPiMF_00069. Hydroxylam_reduct. 1 hit.
InterProiView protein in InterPro
IPR004137. HCP/CODH.
IPR010048. Hydroxylam_reduct.
IPR011254. Prismane-like.
IPR016099. Prismane-like_a/b-sand.
IPR016100. Prismane_a-bundle.
PANTHERiPTHR30109. PTHR30109. 1 hit.
PTHR30109:SF0. PTHR30109:SF0. 1 hit.
PfamiView protein in Pfam
PF03063. Prismane. 1 hit.
PIRSFiPIRSF000076. HCP. 1 hit.
SUPFAMiSSF56821. SSF56821. 1 hit.
TIGRFAMsiTIGR01703. hybrid_clust. 1 hit.

Sequencei

Sequence statusi: Complete.

P75825-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MFCVQCEQTI RTPAGNGCSY AQGMCGKTAE TSDLQDLLIA ALQGLSAWAV
60 70 80 90 100
KAREYGIINH DVDSFAPRAF FSTLTNVNFD SPRIVGYARE AIALREALKA
110 120 130 140 150
QCLAVDANAR VDNPMADLQL VSDDLGELQR QAAEFTPNKD KAAIGENILG
160 170 180 190 200
LRLLCLYGLK GAAAYMEHAH VLGQYDNDIY AQYHKIMAWL GTWPADMNAL
210 220 230 240 250
LECSMEIGQM NFKVMSILDA GETGKYGHPT PTQVNVKATA GKCILISGHD
260 270 280 290 300
LKDLYNLLEQ TEGTGVNVYT HGEMLPAHGY PELRKFKHLV GNYGSGWQNQ
310 320 330 340 350
QVEFARFPGP IVMTSNCIID PTVGAYDDRI WTRSIVGWPG VRHLDGDDFS
360 370 380 390 400
AVITQAQQMA GFPYSEIPHL ITVGFGRQTL LGAADTLIDL VSREKLRHIF
410 420 430 440 450
LLGGCDGARG ERHYFTDFAT SVPDDCLILT LACGKYRFNK LEFGDIEGLP
460 470 480 490 500
RLVDAGQCND AYSAIILAVT LAEKLGCGVN DLPLSLVLSW FEQKAIVILL
510 520 530 540 550
TLLSLGVKNI VTGPTAPGFL TPDLLAVLNE KFGLRSITTV EEDMKQLLSA
Length:550
Mass (Da):60,064
Last modified:May 30, 2000 - v2
Checksum:iCA4958B3143B1F48
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti134E → V in AAC73960 (PubMed:8905232).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00096 Genomic DNA. Translation: AAC73960.2.
AP009048 Genomic DNA. Translation: BAA35587.2.
PIRiA64826.
RefSeqiNP_415394.4. NC_000913.3.
WP_000458809.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC73960; AAC73960; b0873.
BAA35587; BAA35587; BAA35587.
GeneIDi946592.
KEGGiecj:JW0857.
eco:b0873.
PATRICifig|1411691.4.peg.1404.

Similar proteinsi

Entry informationi

Entry nameiHCP_ECOLI
AccessioniPrimary (citable) accession number: P75825
Secondary accession number(s): Q9R7R0, Q9R7R1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: May 30, 2000
Last modified: October 25, 2017
This is version 144 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families