P75824 (HCR_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
NADH oxidoreductase hcr
EC=1.-.-.-

Gene names

Name:	hcr
Synonyms:	ybjV
Ordered Locus Names:	b0872, JW5117

Organism

Escherichia coli (strain K12)

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	322 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	NADH oxidoreductase acting in concert with HCP.
Cofactor	Binds 1 2Fe-2S cluster. FAD.
Sequence similarities	In the N-terminal section; belongs to the FAD-binding oxidoreductase type 6 family. Contains 1 2Fe-2S ferredoxin-type domain. Contains 1 FAD-binding FR-type domain.

Ontologies

Keywords
Biological process	Electron transport Transport
Ligand	2Fe-2S FAD Flavoprotein Iron Iron-sulfur Metal-binding NAD
Molecular function	Oxidoreductase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	electron transport chain Inferred from electronic annotation. Source: UniProtKB-KW transport Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	2 iron, 2 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW electron carrier activity Inferred from electronic annotation. Source: InterPro metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW oxidoreductase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 322	322	NADH oxidoreductase hcr		PRO_0000083928
Regions
	Domain	7 – 107	101	FAD-binding FR-type
	Domain	237 – 322	86	2Fe-2S ferredoxin-type
	Region	111 – 213	103	Oxidoreductase
Sites
	Metal binding	273	1	Iron-sulfur (2Fe-2S) By similarity
	Metal binding	278	1	Iron-sulfur (2Fe-2S) By similarity
	Metal binding	281	1	Iron-sulfur (2Fe-2S) By similarity
	Metal binding	311	1	Iron-sulfur (2Fe-2S) By similarity

Sequences

	Sequence		Length	Mass (Da)
	P75824 [UniParc]. Last modified May 30, 2000. Version 3. Checksum: 5138026DCBBD6A9C	FASTA	322	35,740
	10 20 30 40 50 60 MTMPTNQCPW RMQVHHITQE TPDVWTISLI CHDYYPYRAG QYALVSVRNS AETLRAYTIS 70 80 90 100 110 120 STPGVSEYIT LTVRRIDDGV GSQWLTRDVK RGDYLWLSDA MGEFTCDDKA EDKFLLLAAG 130 140 150 160 170 180 CGVTPIMSMR RWLAKNRPQA DVRVIYNVRT PQDVIFADEW RNYPVTLVAE NNVTEGFIAG 190 200 210 220 230 240 RLTRELLAGV PDLASRTVMT CGPAPYMDWV EQEVKALGVT RFFKEKFFTP VAEAATSGLK 250 260 270 280 290 300 FTKLQPAREF YAPVGTTLLE ALESNNVPVV AACRAGVCGC CKTKVVSGEY TVSSTMTLTD 310 320 AEIAEGYVLA CSCHPQGDLV LA

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"A 718-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 12.7-28.0 min region on the linkage map." Oshima T., Aiba H., Baba T., Fujita K., Hayashi K., Honjo A., Ikemoto K., Inada T., Itoh T., Kajihara M., Kanai K., Kashimoto K., Kimura S., Kitagawa M., Makino K., Masuda S., Miki T., Mizobuchi K. , Mori H., Motomura K., Nakamura Y., Nashimoto H., Nishio Y., Saito N., Sampei G., Seki Y., Tagami H., Takemoto K., Wada C., Yamamoto Y., Yano M., Horiuchi T. DNA Res. 3:137-155(1996) [PubMed: 8905232] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[2]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[3]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed: 16738553] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[4]	"The hybrid-cluster protein ('prismane protein') from Escherichia coli. Characterization of the hybrid-cluster protein, redox properties of the [2Fe-2S] and [4Fe-2S-2O] clusters and identification of an associated NADH oxidoreductase containing FAD and 2Fe-2S." van den Berg W.A.M., Hagen W.R., van Dongen W.M.A.M. Eur. J. Biochem. 267:666-676(2000) [PubMed: 10651802] [Abstract] Cited for: CHARACTERIZATION.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U00096 Genomic DNA. Translation: AAC73959.1. AP009048 Genomic DNA. Translation: BAA35586.2.
PIR	H64825.
RefSeq	NP_415393.1. NC_000913.2.
3D structure databases
ProteinModelPortal	P75824.
SMR	P75824. Positions 7-230, 238-321.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBESCT00000001471; EBESCP00000001471; EBESCG00000001223. EBESCT00000001472; EBESCP00000001472; EBESCG00000001223. EBESCT00000017558; EBESCP00000016849; EBESCG00000016614.
GeneID	947660.
GenomeReviews	Gene locus JW5117 in contig AP009048_GR. Gene locus b0872 in contig U00096_GR.
KEGG	ecj:JW5117. eco:b0872.
PATRIC	32116951. VBIEscCol129921_0901.
Organism-specific databases
EchoBASE	EB3455.
EcoGene	EG13691. hcr.
Phylogenomic databases
eggNOG	COG1018.
GeneTree	EBGT00050000009403.
HOGENOM	HBG483999.
OMA	CHPQSDL.
PhylomeDB	P75824.
ProtClustDB	PRK10684.
Enzyme and pathway databases
BioCyc	EcoCyc:G6456-MONOMER. MetaCyc:G6456-MONOMER.
Gene expression databases
Genevestigator	P75824.
Family and domain databases
InterPro	IPR012675. Beta-grasp_ferredoxin-type. IPR017927. Fd_Rdtase_FAD-bd. IPR001041. Ferredoxin. IPR008333. OxRdtase_FAD-bd_dom. IPR001433. OxRdtase_FAD/NAD-bd. IPR000951. Ph_dOase_redase. IPR017938. Riboflavin_synthase-like_b-brl. [Graphical view]
Gene3D	G3DSA:3.10.20.30. Ferredoxin_fold. 1 hit.
KO	K11933.
Pfam	PF00970. FAD_binding_6. 1 hit. PF00111. Fer2. 1 hit. PF00175. NAD_binding_1. 1 hit. [Graphical view]
PRINTS	PR00409. PHDIOXRDTASE.
SUPFAM	SSF54292. Ferredoxin. 1 hit. SSF63380. Riboflavin_synthase_like_b-brl. 1 hit.
PROSITE	PS00197. 2FE2S_FER_1. False negative. PS51085. 2FE2S_FER_2. 1 hit. PS51384. FAD_FR. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

HCR_ECOLI

Accession

Primary (citable) accession number: P75824

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	May 30, 2000
Last modified:	January 25, 2012
This is version 100 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program