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Protein

N-acetylmuramoyl-L-alanine amidase AmiD

Gene

amiD

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

Hydrolyzes the link between N-acetylmuramoyl residues and L-amino acid residues in certain cell-wall glycopeptides.

Cofactori

Zn2+1 PublicationNote: Binds 1 zinc ion per subunit.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi50Zinc; catalytic1 Publication1
Active sitei119Proton acceptor1 Publication1
Metal bindingi166Zinc; catalytic1 Publication1
Sitei174Transition state stabilizer1
Metal bindingi176Zinc; catalytic1 Publication1

GO - Molecular functioni

  • N-acetyl-anhydromuramoyl-L-alanine amidase activity Source: EcoCyc
  • N-acetylmuramoyl-L-alanine amidase activity Source: EcoCyc
  • zinc ion binding Source: EcoliWiki

GO - Biological processi

  • cell wall organization Source: UniProtKB-KW
  • peptidoglycan catabolic process Source: EcoCyc
  • peptidoglycan turnover Source: GO_Central
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Cell wall biogenesis/degradation

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

BioCyciEcoCyc:G6452-MONOMER.
ECOL316407:JW0851-MONOMER.
MetaCyc:G6452-MONOMER.
BRENDAi3.5.1.28. 2026.

Names & Taxonomyi

Protein namesi
Recommended name:
N-acetylmuramoyl-L-alanine amidase AmiD (EC:3.5.1.28)
Gene namesi
Name:amiD
Synonyms:ybjR
Ordered Locus Names:b0867, JW0851
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG13687. amiD.

Subcellular locationi

GO - Cellular componenti

  • cell outer membrane Source: UniProtKB-SubCell
  • outer membrane Source: EcoliWiki
Complete GO annotation...

Keywords - Cellular componenti

Cell outer membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 16PROSITE-ProRule annotationAdd BLAST16
ChainiPRO_000016441717 – 276N-acetylmuramoyl-L-alanine amidase AmiDAdd BLAST260

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Lipidationi17N-palmitoyl cysteinePROSITE-ProRule annotation1
Lipidationi17S-diacylglycerol cysteinePROSITE-ProRule annotation1

Keywords - PTMi

Lipoprotein, Palmitate

Proteomic databases

PaxDbiP75820.
PRIDEiP75820.

Interactioni

Protein-protein interaction databases

BioGridi4259998. 12 interactors.
STRINGi511145.b0867.

Structurei

Secondary structure

1276
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi23 – 25Combined sources3
Beta strandi30 – 32Combined sources3
Beta strandi46 – 51Combined sources6
Helixi56 – 63Combined sources8
Beta strandi65 – 67Combined sources3
Beta strandi71 – 74Combined sources4
Helixi111 – 114Combined sources4
Beta strandi115 – 120Combined sources6
Beta strandi125 – 128Combined sources4
Beta strandi131 – 134Combined sources4
Helixi139 – 156Combined sources18
Helixi160 – 162Combined sources3
Beta strandi163 – 165Combined sources3
Helixi166 – 169Combined sources4
Turni171 – 173Combined sources3
Helixi183 – 188Combined sources6
Helixi197 – 204Combined sources8
Helixi215 – 225Combined sources11
Helixi235 – 249Combined sources15
Helixi260 – 273Combined sources14

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2BH7X-ray2.20A18-276[»]
2WKXX-ray1.80A18-276[»]
3D2YX-ray1.75A18-276[»]
3D2ZX-ray2.80A18-276[»]
ProteinModelPortaliP75820.
SMRiP75820.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP75820.

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni51 – 52Substrate binding2

Sequence similaritiesi

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiENOG4108VBV. Bacteria.
COG3023. LUCA.
HOGENOMiHOG000255964.
InParanoidiP75820.
KOiK11066.
OMAiHFRPANY.
PhylomeDBiP75820.

Family and domain databases

CDDicd06583. PGRP. 1 hit.
Gene3Di3.40.80.10. 1 hit.
InterProiIPR002502. Amidase_domain.
IPR002477. Peptidoglycan-bd-like.
[Graphical view]
PfamiPF01510. Amidase_2. 1 hit.
[Graphical view]
SMARTiSM00644. Ami_2. 1 hit.
[Graphical view]
SUPFAMiSSF47090. SSF47090. 1 hit.
SSF55846. SSF55846. 1 hit.
PROSITEiPS51257. PROKAR_LIPOPROTEIN. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P75820-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRRFFWLVAA ALLLAGCAGE KGIVEKEGYQ LDTRRQAQAA YPRIKVLVIH
60 70 80 90 100
YTADDFDSSL ATLTDKQVSS HYLVPAVPPR YNGKPRIWQL VPEQELAWHA
110 120 130 140 150
GISAWRGATR LNDTSIGIEL ENRGWQKSAG VKYFAPFEPA QIQALIPLAK
160 170 180 190 200
DIIARYHIKP ENVVAHADIA PQRKDDPGPL FPWQQLAQQG IGAWPDAQRV
210 220 230 240 250
NFYLAGRAPH TPVDTASLLE LLARYGYDVK PDMTPREQRR VIMAFQMHFR
260 270
PTLYNGEADA ETQAIAEALL EKYGQD
Length:276
Mass (Da):31,072
Last modified:February 1, 1997 - v1
Checksum:iE6E4F642AFF548CD
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00096 Genomic DNA. Translation: AAC73954.1.
AP009048 Genomic DNA. Translation: BAA35581.1.
PIRiC64825.
RefSeqiNP_415388.1. NC_000913.3.
WP_001252135.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC73954; AAC73954; b0867.
BAA35581; BAA35581; BAA35581.
GeneIDi945494.
KEGGiecj:JW0851.
eco:b0867.
PATRICi32116941. VBIEscCol129921_0896.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00096 Genomic DNA. Translation: AAC73954.1.
AP009048 Genomic DNA. Translation: BAA35581.1.
PIRiC64825.
RefSeqiNP_415388.1. NC_000913.3.
WP_001252135.1. NZ_LN832404.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2BH7X-ray2.20A18-276[»]
2WKXX-ray1.80A18-276[»]
3D2YX-ray1.75A18-276[»]
3D2ZX-ray2.80A18-276[»]
ProteinModelPortaliP75820.
SMRiP75820.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4259998. 12 interactors.
STRINGi511145.b0867.

Proteomic databases

PaxDbiP75820.
PRIDEiP75820.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC73954; AAC73954; b0867.
BAA35581; BAA35581; BAA35581.
GeneIDi945494.
KEGGiecj:JW0851.
eco:b0867.
PATRICi32116941. VBIEscCol129921_0896.

Organism-specific databases

EchoBASEiEB3451.
EcoGeneiEG13687. amiD.

Phylogenomic databases

eggNOGiENOG4108VBV. Bacteria.
COG3023. LUCA.
HOGENOMiHOG000255964.
InParanoidiP75820.
KOiK11066.
OMAiHFRPANY.
PhylomeDBiP75820.

Enzyme and pathway databases

BioCyciEcoCyc:G6452-MONOMER.
ECOL316407:JW0851-MONOMER.
MetaCyc:G6452-MONOMER.
BRENDAi3.5.1.28. 2026.

Miscellaneous databases

EvolutionaryTraceiP75820.
PROiP75820.

Family and domain databases

CDDicd06583. PGRP. 1 hit.
Gene3Di3.40.80.10. 1 hit.
InterProiIPR002502. Amidase_domain.
IPR002477. Peptidoglycan-bd-like.
[Graphical view]
PfamiPF01510. Amidase_2. 1 hit.
[Graphical view]
SMARTiSM00644. Ami_2. 1 hit.
[Graphical view]
SUPFAMiSSF47090. SSF47090. 1 hit.
SSF55846. SSF55846. 1 hit.
PROSITEiPS51257. PROKAR_LIPOPROTEIN. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiAMID_ECOLI
AccessioniPrimary (citable) accession number: P75820
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: February 1, 1997
Last modified: November 2, 2016
This is version 127 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.