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Protein

23S rRNA (uracil(747)-C(5))-methyltransferase RlmC

Gene

rlmC

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the formation of 5-methyl-uridine at position 747 (m5U747) in 23S rRNA.UniRule annotation1 Publication

Catalytic activityi

S-adenosyl-L-methionine + uracil(747) in 23S rRNA = S-adenosyl-L-homocysteine + 5-methyluracil(747) in 23S rRNA.UniRule annotation1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi3 – 31Iron-sulfur (4Fe-4S)UniRule annotation
Metal bindingi11 – 111Iron-sulfur (4Fe-4S)UniRule annotation
Metal bindingi14 – 141Iron-sulfur (4Fe-4S)UniRule annotation
Metal bindingi87 – 871Iron-sulfur (4Fe-4S)UniRule annotation
Binding sitei212 – 2121S-adenosyl-L-methionineUniRule annotation
Binding sitei241 – 2411S-adenosyl-L-methionine; via carbonyl oxygenUniRule annotation
Binding sitei262 – 2621S-adenosyl-L-methionineUniRule annotation
Binding sitei307 – 3071S-adenosyl-L-methionineUniRule annotation
Active sitei334 – 3341NucleophileUniRule annotation

GO - Molecular functioni

GO - Biological processi

  • rRNA base methylation Source: EcoCyc
Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

rRNA processing

Keywords - Ligandi

4Fe-4S, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciEcoCyc:G6449-MONOMER.
ECOL316407:JW0843-MONOMER.
MetaCyc:G6449-MONOMER.
BRENDAi2.1.1.189. 2026.

Names & Taxonomyi

Protein namesi
Recommended name:
23S rRNA (uracil(747)-C(5))-methyltransferase RlmCUniRule annotation (EC:2.1.1.189UniRule annotation)
Alternative name(s):
23S rRNA(m5U747)-methyltransferaseUniRule annotation
Gene namesi
Name:rlmCUniRule annotation
Synonyms:rumB, ybjF
Ordered Locus Names:b0859, JW0843
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG13427. rlmC.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 37537523S rRNA (uracil(747)-C(5))-methyltransferase RlmCPRO_0000161925Add
BLAST

Proteomic databases

PaxDbiP75817.
PRIDEiP75817.

Interactioni

Protein-protein interaction databases

BioGridi4259987. 8 interactions.
IntActiP75817. 8 interactions.
STRINGi511145.b0859.

Structurei

3D structure databases

ProteinModelPortaliP75817.
SMRiP75817. Positions 209-375.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the class I-like SAM-binding methyltransferase superfamily. RNA M5U methyltransferase family. RlmC subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4107SFS. Bacteria.
COG2265. LUCA.
HOGENOMiHOG000218547.
InParanoidiP75817.
KOiK03212.
OMAiNIQPIHM.
PhylomeDBiP75817.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
HAMAPiMF_01012. 23SrRNA_methyltr_RlmC. 1 hit.
InterProiIPR011825. 23SrRNA_MeTrfase_RlmC.
IPR030390. MeTrfase_TrmA_AS.
IPR030391. MeTrfase_TrmA_CS.
IPR029063. SAM-dependent_MTases.
IPR010280. U5_MeTrfase_fam.
[Graphical view]
PfamiPF05958. tRNA_U5-meth_tr. 1 hit.
[Graphical view]
SUPFAMiSSF53335. SSF53335. 1 hit.
TIGRFAMsiTIGR02085. meth_trns_rumB. 1 hit.
PROSITEiPS51687. SAM_MT_RNA_M5U. 1 hit.
PS01230. TRMA_1. 1 hit.
PS01231. TRMA_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P75817-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MQCALYDAGR CRSCQWIMQP IPEQLSAKTA DLKNLLADFP VEEWCAPVSG
60 70 80 90 100
PEQGFRNKAK MVVSGSVEKP LLGMLHRDGT PEDLCDCPLY PASFAPVFAA
110 120 130 140 150
LKPFIARAGL TPYNVARKRG ELKYILLTES QSDGGMMLRF VLRSDTKLAQ
160 170 180 190 200
LRKALPWLHE QLPQLKVITV NIQPVHMAIM EGETEIYLTE QQALAERFND
210 220 230 240 250
VPLWIRPQSF FQTNPAVASQ LYATARDWVR QLPVKHMWDL FCGVGGFGLH
260 270 280 290 300
CATPDMQLTG IEIASEAIAC AKQSAAELGL TRLQFQALDS TQFATAQGDV
310 320 330 340 350
PELVLVNPPR RGIGKPLCDY LSTMAPRFII YSSCNAQTMA KDIRELPGFR
360 370
IERVQLFDMF PHTAHYEVLT LLVKQ
Length:375
Mass (Da):41,956
Last modified:February 1, 1997 - v1
Checksum:iEDFE591BE2B9DE7D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00096 Genomic DNA. Translation: AAC73946.1.
AP009048 Genomic DNA. Translation: BAA35573.1.
PIRiC64824.
RefSeqiNP_415380.1. NC_000913.3.
WP_001149682.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC73946; AAC73946; b0859.
BAA35573; BAA35573; BAA35573.
GeneIDi947260.
KEGGiecj:JW0843.
eco:b0859.
PATRICi32116923. VBIEscCol129921_0887.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00096 Genomic DNA. Translation: AAC73946.1.
AP009048 Genomic DNA. Translation: BAA35573.1.
PIRiC64824.
RefSeqiNP_415380.1. NC_000913.3.
WP_001149682.1. NZ_LN832404.1.

3D structure databases

ProteinModelPortaliP75817.
SMRiP75817. Positions 209-375.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4259987. 8 interactions.
IntActiP75817. 8 interactions.
STRINGi511145.b0859.

Proteomic databases

PaxDbiP75817.
PRIDEiP75817.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC73946; AAC73946; b0859.
BAA35573; BAA35573; BAA35573.
GeneIDi947260.
KEGGiecj:JW0843.
eco:b0859.
PATRICi32116923. VBIEscCol129921_0887.

Organism-specific databases

EchoBASEiEB3201.
EcoGeneiEG13427. rlmC.

Phylogenomic databases

eggNOGiENOG4107SFS. Bacteria.
COG2265. LUCA.
HOGENOMiHOG000218547.
InParanoidiP75817.
KOiK03212.
OMAiNIQPIHM.
PhylomeDBiP75817.

Enzyme and pathway databases

BioCyciEcoCyc:G6449-MONOMER.
ECOL316407:JW0843-MONOMER.
MetaCyc:G6449-MONOMER.
BRENDAi2.1.1.189. 2026.

Miscellaneous databases

PROiP75817.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
HAMAPiMF_01012. 23SrRNA_methyltr_RlmC. 1 hit.
InterProiIPR011825. 23SrRNA_MeTrfase_RlmC.
IPR030390. MeTrfase_TrmA_AS.
IPR030391. MeTrfase_TrmA_CS.
IPR029063. SAM-dependent_MTases.
IPR010280. U5_MeTrfase_fam.
[Graphical view]
PfamiPF05958. tRNA_U5-meth_tr. 1 hit.
[Graphical view]
SUPFAMiSSF53335. SSF53335. 1 hit.
TIGRFAMsiTIGR02085. meth_trns_rumB. 1 hit.
PROSITEiPS51687. SAM_MT_RNA_M5U. 1 hit.
PS01230. TRMA_1. 1 hit.
PS01231. TRMA_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiRLMC_ECOLI
AccessioniPrimary (citable) accession number: P75817
Secondary accession number(s): Q9R7R3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: February 1, 1997
Last modified: September 7, 2016
This is version 115 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.