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Protein

23S rRNA (uracil(747)-C(5))-methyltransferase RlmC

Gene

rlmC

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes the formation of 5-methyl-uridine at position 747 (m5U747) in 23S rRNA.UniRule annotation1 Publication

Catalytic activityi

S-adenosyl-L-methionine + uracil(747) in 23S rRNA = S-adenosyl-L-homocysteine + 5-methyluracil(747) in 23S rRNA.UniRule annotation1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi3Iron-sulfur (4Fe-4S)UniRule annotation1
Metal bindingi11Iron-sulfur (4Fe-4S)UniRule annotation1
Metal bindingi14Iron-sulfur (4Fe-4S)UniRule annotation1
Metal bindingi87Iron-sulfur (4Fe-4S)UniRule annotation1
Binding sitei212S-adenosyl-L-methionineUniRule annotation1
Binding sitei241S-adenosyl-L-methionine; via carbonyl oxygenUniRule annotation1
Binding sitei262S-adenosyl-L-methionineUniRule annotation1
Binding sitei307S-adenosyl-L-methionineUniRule annotation1
Active sitei334NucleophileUniRule annotation1

GO - Molecular functioni

  • 4 iron, 4 sulfur cluster binding Source: UniProtKB-KW
  • metal ion binding Source: UniProtKB-KW
  • rRNA (uridine) methyltransferase activity Source: EcoCyc

GO - Biological processi

  • rRNA base methylation Source: EcoCyc

Keywordsi

Molecular functionMethyltransferase, Transferase
Biological processrRNA processing
Ligand4Fe-4S, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciEcoCyc:G6449-MONOMER
MetaCyc:G6449-MONOMER
BRENDAi2.1.1.189 2026

Names & Taxonomyi

Protein namesi
Recommended name:
23S rRNA (uracil(747)-C(5))-methyltransferase RlmCUniRule annotation (EC:2.1.1.189UniRule annotation)
Alternative name(s):
23S rRNA(m5U747)-methyltransferaseUniRule annotation
Gene namesi
Name:rlmCUniRule annotation
Synonyms:rumB, ybjF
Ordered Locus Names:b0859, JW0843
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG13427 rlmC

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001619251 – 37523S rRNA (uracil(747)-C(5))-methyltransferase RlmCAdd BLAST375

Proteomic databases

PaxDbiP75817
PRIDEiP75817

Interactioni

Protein-protein interaction databases

BioGridi4259987, 24 interactors
IntActiP75817, 8 interactors
STRINGi316385.ECDH10B_0929

Structurei

3D structure databases

ProteinModelPortaliP75817
SMRiP75817
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the class I-like SAM-binding methyltransferase superfamily. RNA M5U methyltransferase family. RlmC subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4107SFS Bacteria
COG2265 LUCA
HOGENOMiHOG000218547
InParanoidiP75817
KOiK03212
OMAiSCQWLEK
PhylomeDBiP75817

Family and domain databases

HAMAPiMF_01012 23SrRNA_methyltr_RlmC, 1 hit
InterProiView protein in InterPro
IPR011825 23SrRNA_MeTrfase_RlmC
IPR030390 MeTrfase_TrmA_AS
IPR030391 MeTrfase_TrmA_CS
IPR029063 SAM-dependent_MTases
IPR010280 U5_MeTrfase_fam
PANTHERiPTHR11061 PTHR11061, 1 hit
PfamiView protein in Pfam
PF05958 tRNA_U5-meth_tr, 1 hit
SUPFAMiSSF53335 SSF53335, 1 hit
TIGRFAMsiTIGR02085 meth_trns_rumB, 1 hit
PROSITEiView protein in PROSITE
PS51687 SAM_MT_RNA_M5U, 1 hit
PS01230 TRMA_1, 1 hit
PS01231 TRMA_2, 1 hit

Sequencei

Sequence statusi: Complete.

P75817-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MQCALYDAGR CRSCQWIMQP IPEQLSAKTA DLKNLLADFP VEEWCAPVSG
60 70 80 90 100
PEQGFRNKAK MVVSGSVEKP LLGMLHRDGT PEDLCDCPLY PASFAPVFAA
110 120 130 140 150
LKPFIARAGL TPYNVARKRG ELKYILLTES QSDGGMMLRF VLRSDTKLAQ
160 170 180 190 200
LRKALPWLHE QLPQLKVITV NIQPVHMAIM EGETEIYLTE QQALAERFND
210 220 230 240 250
VPLWIRPQSF FQTNPAVASQ LYATARDWVR QLPVKHMWDL FCGVGGFGLH
260 270 280 290 300
CATPDMQLTG IEIASEAIAC AKQSAAELGL TRLQFQALDS TQFATAQGDV
310 320 330 340 350
PELVLVNPPR RGIGKPLCDY LSTMAPRFII YSSCNAQTMA KDIRELPGFR
360 370
IERVQLFDMF PHTAHYEVLT LLVKQ
Length:375
Mass (Da):41,956
Last modified:February 1, 1997 - v1
Checksum:iEDFE591BE2B9DE7D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00096 Genomic DNA Translation: AAC73946.1
AP009048 Genomic DNA Translation: BAA35573.1
PIRiC64824
RefSeqiNP_415380.1, NC_000913.3
WP_001149682.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaiAAC73946; AAC73946; b0859
BAA35573; BAA35573; BAA35573
GeneIDi947260
KEGGiecj:JW0843
eco:b0859
PATRICifig|1411691.4.peg.1418

Similar proteinsi

Entry informationi

Entry nameiRLMC_ECOLI
AccessioniPrimary (citable) accession number: P75817
Secondary accession number(s): Q9R7R3
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: February 1, 1997
Last modified: March 28, 2018
This is version 126 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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