P75817 (RLMC_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
23S rRNA (uracil(747)-C(5))-methyltransferase RlmC
EC=2.1.1.189

Alternative name(s):
23S rRNA(m5U747)-methyltransferase

Gene names

Name:	rlmC
Synonyms:	rumB, ybjF
Ordered Locus Names:	b0859, JW0843

Organism

Escherichia coli (strain K12)

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	375 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Catalyzes the formation of 5-methyl-uridine at position 747 (m5U747) in 23S rRNA. Ref.4
Catalytic activity	S-adenosyl-L-methionine + uracil(747) in 23S rRNA = S-adenosyl-L-homocysteine + 5-methyluracil(747) in 23S rRNA. Ref.4
Sequence similarities	Belongs to the methyltransferase superfamily. RNA M5U methyltransferase family. RlmC subfamily.

Ontologies

Keywords
Biological process	rRNA processing
Ligand	4Fe-4S Iron Iron-sulfur Metal-binding S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Molecular function	4 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW rRNA (uridine) methyltransferase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 375	375	23S rRNA (uracil(747)-C(5))-methyltransferase RlmC HAMAP MF_01012		PRO_0000161925
Sites
	Active site	334	1	Nucleophile By similarity
	Metal binding	3	1	Iron-sulfur (4Fe-4S) By similarity
	Metal binding	11	1	Iron-sulfur (4Fe-4S) By similarity
	Metal binding	14	1	Iron-sulfur (4Fe-4S) By similarity
	Metal binding	87	1	Iron-sulfur (4Fe-4S) By similarity
	Binding site	212	1	S-adenosyl-L-methionine By similarity
	Binding site	241	1	S-adenosyl-L-methionine; via carbonyl oxygen By similarity
	Binding site	262	1	S-adenosyl-L-methionine By similarity
	Binding site	307	1	S-adenosyl-L-methionine By similarity

Sequences

	Sequence		Length	Mass (Da)
	P75817 [UniParc]. Last modified February 1, 1997. Version 1. Checksum: EDFE591BE2B9DE7D	FASTA	375	41,956
	10 20 30 40 50 60 MQCALYDAGR CRSCQWIMQP IPEQLSAKTA DLKNLLADFP VEEWCAPVSG PEQGFRNKAK 70 80 90 100 110 120 MVVSGSVEKP LLGMLHRDGT PEDLCDCPLY PASFAPVFAA LKPFIARAGL TPYNVARKRG 130 140 150 160 170 180 ELKYILLTES QSDGGMMLRF VLRSDTKLAQ LRKALPWLHE QLPQLKVITV NIQPVHMAIM 190 200 210 220 230 240 EGETEIYLTE QQALAERFND VPLWIRPQSF FQTNPAVASQ LYATARDWVR QLPVKHMWDL 250 260 270 280 290 300 FCGVGGFGLH CATPDMQLTG IEIASEAIAC AKQSAAELGL TRLQFQALDS TQFATAQGDV 310 320 330 340 350 360 PELVLVNPPR RGIGKPLCDY LSTMAPRFII YSSCNAQTMA KDIRELPGFR IERVQLFDMF 370 PHTAHYEVLT LLVKQ

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"A 718-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 12.7-28.0 min region on the linkage map." Oshima T., Aiba H., Baba T., Fujita K., Hayashi K., Honjo A., Ikemoto K., Inada T., Itoh T., Kajihara M., Kanai K., Kashimoto K., Kimura S., Kitagawa M., Makino K., Masuda S., Miki T., Mizobuchi K. , Mori H., Motomura K., Nakamura Y., Nashimoto H., Nishio Y., Saito N., Sampei G., Seki Y., Tagami H., Takemoto K., Wada C., Yamamoto Y., Yano M., Horiuchi T. DNA Res. 3:137-155(1996) [PubMed: 8905232] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[2]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[3]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed: 16738553] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[4]	"Identifying the methyltransferases for m(5)U747 and m(5)U1939 in 23S rRNA using MALDI mass spectrometry." Madsen C.T., Mengel-Joergensen J., Kirpekar F., Douthwaite S. Nucleic Acids Res. 31:4738-4746(2003) [PubMed: 12907714] [Abstract] Cited for: FUNCTION, CATALYTIC ACTIVITY.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U00096 Genomic DNA. Translation: AAC73946.1. AP009048 Genomic DNA. Translation: BAA35573.1.
PIR	C64824.
RefSeq	NP_415380.1. NC_000913.2.
3D structure databases
ProteinModelPortal	P75817.
SMR	P75817. Positions 2-373.
ModBase	Search...
Protein-protein interaction databases
IntAct	P75817. 8 interactions.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBESCT00000003640; EBESCP00000003640; EBESCG00000002973. EBESCT00000017223; EBESCP00000016514; EBESCG00000016282.
GeneID	947260.
GenomeReviews	Gene locus JW0843 in contig AP009048_GR. Gene locus b0859 in contig U00096_GR.
KEGG	ecj:JW0843. eco:b0859.
PATRIC	32116923. VBIEscCol129921_0887.
Organism-specific databases
EchoBASE	EB3201.
EcoGene	EG13427. rlmC.
Phylogenomic databases
eggNOG	COG2265.
GeneTree	EBGT00050000009928.
HOGENOM	HBG296593.
OMA	IMEGERE.
PhylomeDB	P75817.
ProtClustDB	PRK03522.
Enzyme and pathway databases
BioCyc	EcoCyc:G6449-MONOMER. MetaCyc:G6449-MONOMER.
Gene expression databases
Genevestigator	P75817.
Family and domain databases
HAMAP	MF_01012. 23SrRNA_methyltr_RlmC. [Tree]
InterPro	IPR011825. MeTrfase_RumB. IPR010280. U5_MeTrfase. [Graphical view]
KO	K03212.
Pfam	PF05958. tRNA_U5-meth_tr. 1 hit. [Graphical view]
TIGRFAMs	TIGR02085. Meth_trns_rumB. 1 hit.
PROSITE	PS01230. TRMA_1. 1 hit. PS01231. TRMA_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

RLMC_ECOLI

Accession

Primary (citable) accession number: P75817
Secondary accession number(s): Q9R7R3

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	February 1, 1997
Last modified:	January 25, 2012
This is version 85 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program