P75809 (YBJI_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Flavin mononucleotide phosphatase YbjI
EC=3.1.3.-

Alternative name(s):
FMN phosphatase

Gene names

Name:	ybjI
Ordered Locus Names:	b0844, JW5113

Organism

Escherichia coli (strain K12) [Reference proteome] [HAMAP]

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia › Escherichia coli

Protein attributes

Sequence length	271 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Catalyzes the dephosphorylation of flavin mononucleotide (FMN): Can also hydrolyze erythrose 4-phosphate (Ery4P). Ref.5 Ref.6
Cofactor	Magnesium. Can also use other divalent metal cations as manganese, cobalt and zinc. Ref.6
Sequence similarities	Belongs to the HAD-like hydrolase superfamily. Cof family.
Biophysicochemical properties	Kinetic parameters: KM=2.3 mM for FMN (with magnesium ions as cofactor and at pH 9) Ref.6 KM=2.6 mM for Ery4P (with magnesium ions as cofactor and at pH 9) pH dependence: Optimum pH is between 6 and 7.5.

Ontologies

Keywords
Ligand	Magnesium Metal-binding
Molecular function	Hydrolase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	FMN catabolic process Inferred from direct assay Ref.6. Source: EcoCyc
Molecular_function	magnesium ion binding Inferred from direct assay Ref.6. Source: UniProtKB phosphatase activity Inferred from direct assay Ref.6. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 271	271	Flavin mononucleotide phosphatase YbjI		PRO_0000054422
Regions
	Region	9 – 11	3	Substrate By similarity
Sites
	Active site	9	1	Nucleophile By similarity
	Metal binding	9	1	Magnesium By similarity
	Metal binding	11	1	Magnesium By similarity
	Metal binding	215	1	Magnesium By similarity

Sequences

	Sequence		Length	Mass (Da)
	P75809 [UniParc]. Last modified May 30, 2000. Version 3. Checksum: AF44E565BCB68BA9	FASTA	271	30,196
	10 20 30 40 50 60 MSIKLIAVDM DGTFLSDQKT YNRERFMAQY QQMKAQGIRF VVASGNQYYQ LISFFPEIAN 70 80 90 100 110 120 EIAFVAENGG WVVSEGKDVF NGELSKDAFA TVVEHLLTRP EVEIIACGKN SAYTLKKYDD 130 140 150 160 170 180 AMKTVAEMYY HRLEYVDNFD NLEDIFFKFG LNLSDELIPQ VQKALHEAIG DIMVSVHTGN 190 200 210 220 230 240 GSIDLIIPGV HKANGLRQLQ KLWGIDDSEV VVFGDGGNDI EMLRQAGFSF AMENAGSAVV 250 260 270 AAAKYRAGSN NREGVLDVID KVLKHEAPFD Q

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"A 718-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 12.7-28.0 min region on the linkage map." Oshima T., Aiba H., Baba T., Fujita K., Hayashi K., Honjo A., Ikemoto K., Inada T., Itoh T., Kajihara M., Kanai K., Kashimoto K., Kimura S., Kitagawa M., Makino K., Masuda S., Miki T., Mizobuchi K. , Mori H., Motomura K., Nakamura Y., Nashimoto H., Nishio Y., Saito N., Sampei G., Seki Y., Tagami H., Takemoto K., Wada C., Yamamoto Y., Yano M., Horiuchi T. DNA Res. 3:137-155(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[2]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[3]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[4]	"Enrichment of low abundance proteins of Escherichia coli by hydroxyapatite chromatography." Fountoulakis M., Takacs M.-F., Berndt P., Langen H., Takacs B. Electrophoresis 20:2181-2195(1999) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY. Strain: B / BL21.
[5]	"Enzyme genomics: application of general enzymatic screens to discover new enzymes." Kuznetsova E., Proudfoot M., Sanders S.A., Reinking J., Savchenko A., Arrowsmith C.H., Edwards A.M., Yakunin A.F. FEMS Microbiol. Rev. 29:263-279(2005) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION AS A PHOSPHATASE.
[6]	"Genome-wide analysis of substrate specificities of the Escherichia coli haloacid dehalogenase-like phosphatase family." Kuznetsova E., Proudfoot M., Gonzalez C.F., Brown G., Omelchenko M.V., Borozan I., Carmel L., Wolf Y.I., Mori H., Savchenko A.V., Arrowsmith C.H., Koonin E.V., Edwards A.M., Yakunin A.F. J. Biol. Chem. 281:36149-36161(2006) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION AS A PHOSPHATASE, BIOPHYSICOCHEMICAL PROPERTIES, SUBSTRATE SPECIFICITY, COFACTOR.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U00096 Genomic DNA. Translation: AAC73931.2. AP009048 Genomic DNA. Translation: BAA35548.2.
PIR	D64822.
RefSeq	NP_415365.4. NC_000913.2. YP_489117.1. NC_007779.1.
3D structure databases
ProteinModelPortal	P75809.
SMR	P75809. Positions 6-270.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-11451N.
STRING	511145.b0844.
Proteomic databases
PaxDb	P75809.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAC73931; AAC73931; b0844. BAA35548; BAA35548; BAA35548.
GeneID	12933836. 945470.
KEGG	ecj:Y75_p0817. eco:b0844.
PATRIC	32116893. VBIEscCol129921_0872.
Organism-specific databases
EchoBASE	EB3442.
EcoGene	EG13678. ybjI.
Phylogenomic databases
eggNOG	COG0561.
HOGENOM	HOG000184782.
KO	K07024.
OMA	KAVASHH.
ProtClustDB	CLSK879789.
Enzyme and pathway databases
BioCyc	EcoCyc:G6442-MONOMER. ECOL316407:JW5113-MONOMER. MetaCyc:G6442-MONOMER.
Gene expression databases
Genevestigator	P75809.
Family and domain databases
Gene3D	3.40.50.1000. 2 hits.
InterPro	IPR023214. HAD-like_dom. IPR006379. HAD-SF_hydro_IIB. IPR000150. Hypothet_cof. [Graphical view]
Pfam	PF00702. Hydrolase. 1 hit. [Graphical view]
SUPFAM	SSF56784. HAD-like_dom. 1 hit.
TIGRFAMs	TIGR00099. Cof-subfamily. 1 hit. TIGR01484. HAD-SF-IIB. 1 hit.
PROSITE	PS01228. COF_1. False negative. PS01229. COF_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

YBJI_ECOLI

Accession

Primary (citable) accession number: P75809

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	May 30, 2000
Last modified:	May 1, 2013
This is version 94 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program