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Protein

Glutathione-binding protein GsiB

Gene

gsiB

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Part of the ABC transporter complex GsiABCD involved in glutathione import.1 Publication

GO - Molecular functioni

  • glutathione transmembrane transporter activity Source: EcoCyc

GO - Biological processi

  • cellular response to DNA damage stimulus Source: EcoliWiki
  • glutathione transmembrane transport Source: EcoCyc
Complete GO annotation...

Keywords - Biological processi

Transport

Enzyme and pathway databases

BioCyciEcoCyc:YLIB-MONOMER.
ECOL316407:JW5111-MONOMER.
MetaCyc:YLIB-MONOMER.

Protein family/group databases

TCDBi3.A.1.5.11. the atp-binding cassette (abc) superfamily.

Names & Taxonomyi

Protein namesi
Recommended name:
Glutathione-binding protein GsiB
Gene namesi
Name:gsiB
Synonyms:yliB
Ordered Locus Names:b0830, JW5111
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG13473. gsiB.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Periplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2626Sequence analysisAdd
BLAST
Chaini27 – 512486Glutathione-binding protein GsiBPRO_0000031807Add
BLAST

Proteomic databases

EPDiP75797.
PaxDbiP75797.
PRIDEiP75797.

2D gel databases

SWISS-2DPAGEP75797.

Interactioni

Subunit structurei

The complex is composed of two ATP-binding proteins (GsiA), two transmembrane proteins (GsiC and GsiD) and a solute-binding protein (GsiB).Curated

Protein-protein interaction databases

BioGridi4259982. 13 interactions.
DIPiDIP-12697N.
STRINGi511145.b0830.

Structurei

Secondary structure

1
512
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi29 – 368Combined sources
Helixi43 – 453Combined sources
Helixi49 – 557Combined sources
Turni56 – 583Combined sources
Beta strandi62 – 654Combined sources
Beta strandi71 – 8111Combined sources
Beta strandi85 – 928Combined sources
Helixi106 – 11611Combined sources
Helixi119 – 1213Combined sources
Helixi126 – 1283Combined sources
Turni129 – 1313Combined sources
Beta strandi132 – 1398Combined sources
Beta strandi142 – 1498Combined sources
Helixi154 – 1585Combined sources
Helixi168 – 1747Combined sources
Helixi175 – 1773Combined sources
Turni178 – 1803Combined sources
Beta strandi185 – 19410Combined sources
Turni195 – 1973Combined sources
Beta strandi198 – 2036Combined sources
Beta strandi216 – 2227Combined sources
Helixi226 – 23510Combined sources
Beta strandi239 – 2413Combined sources
Helixi246 – 2549Combined sources
Beta strandi258 – 27316Combined sources
Helixi279 – 2813Combined sources
Helixi283 – 29210Combined sources
Helixi295 – 3039Combined sources
Beta strandi307 – 3093Combined sources
Beta strandi311 – 3144Combined sources
Helixi331 – 34111Combined sources
Beta strandi347 – 3548Combined sources
Beta strandi357 – 3593Combined sources
Helixi360 – 37314Combined sources
Beta strandi376 – 3838Combined sources
Helixi385 – 3917Combined sources
Turni397 – 3993Combined sources
Beta strandi404 – 4107Combined sources
Helixi416 – 4249Combined sources
Helixi426 – 4283Combined sources
Turni429 – 4324Combined sources
Helixi442 – 45211Combined sources
Helixi457 – 47418Combined sources
Beta strandi477 – 49014Combined sources
Beta strandi493 – 4953Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1UQWX-ray2.72A/B24-512[»]
ProteinModelPortaliP75797.
SMRiP75797. Positions 26-512.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP75797.

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiENOG4105DEF. Bacteria.
COG0747. LUCA.
HOGENOMiHOG000164955.
InParanoidiP75797.
KOiK13889.
OMAiKESPWVP.
PhylomeDBiP75797.

Family and domain databases

InterProiIPR030678. Peptide/Ni-bd.
IPR023765. SBP_5_CS.
IPR000914. SBP_5_dom.
[Graphical view]
PfamiPF00496. SBP_bac_5. 1 hit.
[Graphical view]
PIRSFiPIRSF002741. MppA. 1 hit.
PROSITEiPS01040. SBP_BACTERIAL_5. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P75797-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MARAVHRSGL VALGIATALM ASCAFAAKDV VVAVGSNFTT LDPYDANDTL
60 70 80 90 100
SQAVAKSFYQ GLFGLDKEMK LKNVLAESYT VSDDGITYTV KLREGIKFQD
110 120 130 140 150
GTDFNAAAVK ANLDRASDPA NHLKRYNLYK NIAKTEAIDP TTVKITLKQP
160 170 180 190 200
FSAFINILAH PATAMISPAA LEKYGKEIGF YPVGTGPYEL DTWNQTDFVK
210 220 230 240 250
VKKFAGYWQP GLPKLDSITW RPVADNNTRA AMLQTGEAQF AFPIPYEQAT
260 270 280 290 300
LLEKNKNIEL MASPSIMQRY ISMNVTQKPF DNPKVREALN YAINRPALVK
310 320 330 340 350
VAFAGYATPA TGVVPPSIAY AQSYKPWPYD PVKARELLKE AGYPNGFSTT
360 370 380 390 400
LWSSHNHSTA QKVLQFTQQQ LAQVGIKAQV TAMDAGQRAA EVEGKGQKES
410 420 430 440 450
GVRMFYTGWS ASTGEADWAL SPLFASQNWP PTLFNTAFYS NKQVDDFLAQ
460 470 480 490 500
ALKTNDPAEK TRLYKAAQDI IWQESPWIPL VVEKLVSAHS KNLTGFWIMP
510
DTGFSFEDAD LQ
Length:512
Mass (Da):56,470
Last modified:February 1, 1997 - v1
Checksum:iF7552060CA4FBF86
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00096 Genomic DNA. Translation: AAC73917.1.
AP009048 Genomic DNA. Translation: BAA35525.2.
PIRiF64820.
RefSeqiNP_415351.1. NC_000913.3.
WP_000090140.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC73917; AAC73917; b0830.
BAA35525; BAA35525; BAA35525.
GeneIDi945459.
KEGGiecj:JW5111.
eco:b0830.
PATRICi32116863. VBIEscCol129921_0857.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00096 Genomic DNA. Translation: AAC73917.1.
AP009048 Genomic DNA. Translation: BAA35525.2.
PIRiF64820.
RefSeqiNP_415351.1. NC_000913.3.
WP_000090140.1. NZ_LN832404.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1UQWX-ray2.72A/B24-512[»]
ProteinModelPortaliP75797.
SMRiP75797. Positions 26-512.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4259982. 13 interactions.
DIPiDIP-12697N.
STRINGi511145.b0830.

Protein family/group databases

TCDBi3.A.1.5.11. the atp-binding cassette (abc) superfamily.

2D gel databases

SWISS-2DPAGEP75797.

Proteomic databases

EPDiP75797.
PaxDbiP75797.
PRIDEiP75797.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC73917; AAC73917; b0830.
BAA35525; BAA35525; BAA35525.
GeneIDi945459.
KEGGiecj:JW5111.
eco:b0830.
PATRICi32116863. VBIEscCol129921_0857.

Organism-specific databases

EchoBASEiEB3246.
EcoGeneiEG13473. gsiB.

Phylogenomic databases

eggNOGiENOG4105DEF. Bacteria.
COG0747. LUCA.
HOGENOMiHOG000164955.
InParanoidiP75797.
KOiK13889.
OMAiKESPWVP.
PhylomeDBiP75797.

Enzyme and pathway databases

BioCyciEcoCyc:YLIB-MONOMER.
ECOL316407:JW5111-MONOMER.
MetaCyc:YLIB-MONOMER.

Miscellaneous databases

EvolutionaryTraceiP75797.
PROiP75797.

Family and domain databases

InterProiIPR030678. Peptide/Ni-bd.
IPR023765. SBP_5_CS.
IPR000914. SBP_5_dom.
[Graphical view]
PfamiPF00496. SBP_bac_5. 1 hit.
[Graphical view]
PIRSFiPIRSF002741. MppA. 1 hit.
PROSITEiPS01040. SBP_BACTERIAL_5. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiGSIB_ECOLI
AccessioniPrimary (citable) accession number: P75797
Secondary accession number(s): Q9R7R5, Q9R7R7, Q9R7R8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: February 1, 1997
Last modified: September 7, 2016
This is version 128 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.