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Protein

Glutathione-binding protein GsiB

Gene

gsiB

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Part of the ABC transporter complex GsiABCD involved in glutathione import.1 Publication

GO - Molecular functioni

  • glutathione transmembrane transporter activity Source: EcoCyc

GO - Biological processi

  • cellular response to DNA damage stimulus Source: EcoliWiki
  • glutathione transmembrane transport Source: EcoCyc
Complete GO annotation...

Keywords - Biological processi

Transport

Enzyme and pathway databases

BioCyciEcoCyc:YLIB-MONOMER.
ECOL316407:JW5111-MONOMER.
MetaCyc:YLIB-MONOMER.

Protein family/group databases

TCDBi3.A.1.5.11. the atp-binding cassette (abc) superfamily.

Names & Taxonomyi

Protein namesi
Recommended name:
Glutathione-binding protein GsiB
Gene namesi
Name:gsiB
Synonyms:yliB
Ordered Locus Names:b0830, JW5111
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG13473. gsiB.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Periplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 26Sequence analysisAdd BLAST26
ChainiPRO_000003180727 – 512Glutathione-binding protein GsiBAdd BLAST486

Proteomic databases

EPDiP75797.
PaxDbiP75797.
PRIDEiP75797.

2D gel databases

SWISS-2DPAGEP75797.

Interactioni

Subunit structurei

The complex is composed of two ATP-binding proteins (GsiA), two transmembrane proteins (GsiC and GsiD) and a solute-binding protein (GsiB).Curated

Protein-protein interaction databases

BioGridi4259982. 13 interactors.
DIPiDIP-12697N.
STRINGi511145.b0830.

Structurei

Secondary structure

1512
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi29 – 36Combined sources8
Helixi43 – 45Combined sources3
Helixi49 – 55Combined sources7
Turni56 – 58Combined sources3
Beta strandi62 – 65Combined sources4
Beta strandi71 – 81Combined sources11
Beta strandi85 – 92Combined sources8
Helixi106 – 116Combined sources11
Helixi119 – 121Combined sources3
Helixi126 – 128Combined sources3
Turni129 – 131Combined sources3
Beta strandi132 – 139Combined sources8
Beta strandi142 – 149Combined sources8
Helixi154 – 158Combined sources5
Helixi168 – 174Combined sources7
Helixi175 – 177Combined sources3
Turni178 – 180Combined sources3
Beta strandi185 – 194Combined sources10
Turni195 – 197Combined sources3
Beta strandi198 – 203Combined sources6
Beta strandi216 – 222Combined sources7
Helixi226 – 235Combined sources10
Beta strandi239 – 241Combined sources3
Helixi246 – 254Combined sources9
Beta strandi258 – 273Combined sources16
Helixi279 – 281Combined sources3
Helixi283 – 292Combined sources10
Helixi295 – 303Combined sources9
Beta strandi307 – 309Combined sources3
Beta strandi311 – 314Combined sources4
Helixi331 – 341Combined sources11
Beta strandi347 – 354Combined sources8
Beta strandi357 – 359Combined sources3
Helixi360 – 373Combined sources14
Beta strandi376 – 383Combined sources8
Helixi385 – 391Combined sources7
Turni397 – 399Combined sources3
Beta strandi404 – 410Combined sources7
Helixi416 – 424Combined sources9
Helixi426 – 428Combined sources3
Turni429 – 432Combined sources4
Helixi442 – 452Combined sources11
Helixi457 – 474Combined sources18
Beta strandi477 – 490Combined sources14
Beta strandi493 – 495Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1UQWX-ray2.72A/B24-512[»]
ProteinModelPortaliP75797.
SMRiP75797.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP75797.

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiENOG4105DEF. Bacteria.
COG0747. LUCA.
HOGENOMiHOG000164955.
InParanoidiP75797.
KOiK13889.
OMAiKESPWVP.
PhylomeDBiP75797.

Family and domain databases

InterProiIPR030678. Peptide/Ni-bd.
IPR023765. SBP_5_CS.
IPR000914. SBP_5_dom.
[Graphical view]
PfamiPF00496. SBP_bac_5. 1 hit.
[Graphical view]
PIRSFiPIRSF002741. MppA. 1 hit.
PROSITEiPS01040. SBP_BACTERIAL_5. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P75797-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MARAVHRSGL VALGIATALM ASCAFAAKDV VVAVGSNFTT LDPYDANDTL
60 70 80 90 100
SQAVAKSFYQ GLFGLDKEMK LKNVLAESYT VSDDGITYTV KLREGIKFQD
110 120 130 140 150
GTDFNAAAVK ANLDRASDPA NHLKRYNLYK NIAKTEAIDP TTVKITLKQP
160 170 180 190 200
FSAFINILAH PATAMISPAA LEKYGKEIGF YPVGTGPYEL DTWNQTDFVK
210 220 230 240 250
VKKFAGYWQP GLPKLDSITW RPVADNNTRA AMLQTGEAQF AFPIPYEQAT
260 270 280 290 300
LLEKNKNIEL MASPSIMQRY ISMNVTQKPF DNPKVREALN YAINRPALVK
310 320 330 340 350
VAFAGYATPA TGVVPPSIAY AQSYKPWPYD PVKARELLKE AGYPNGFSTT
360 370 380 390 400
LWSSHNHSTA QKVLQFTQQQ LAQVGIKAQV TAMDAGQRAA EVEGKGQKES
410 420 430 440 450
GVRMFYTGWS ASTGEADWAL SPLFASQNWP PTLFNTAFYS NKQVDDFLAQ
460 470 480 490 500
ALKTNDPAEK TRLYKAAQDI IWQESPWIPL VVEKLVSAHS KNLTGFWIMP
510
DTGFSFEDAD LQ
Length:512
Mass (Da):56,470
Last modified:February 1, 1997 - v1
Checksum:iF7552060CA4FBF86
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00096 Genomic DNA. Translation: AAC73917.1.
AP009048 Genomic DNA. Translation: BAA35525.2.
PIRiF64820.
RefSeqiNP_415351.1. NC_000913.3.
WP_000090140.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC73917; AAC73917; b0830.
BAA35525; BAA35525; BAA35525.
GeneIDi945459.
KEGGiecj:JW5111.
eco:b0830.
PATRICi32116863. VBIEscCol129921_0857.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00096 Genomic DNA. Translation: AAC73917.1.
AP009048 Genomic DNA. Translation: BAA35525.2.
PIRiF64820.
RefSeqiNP_415351.1. NC_000913.3.
WP_000090140.1. NZ_LN832404.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1UQWX-ray2.72A/B24-512[»]
ProteinModelPortaliP75797.
SMRiP75797.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4259982. 13 interactors.
DIPiDIP-12697N.
STRINGi511145.b0830.

Protein family/group databases

TCDBi3.A.1.5.11. the atp-binding cassette (abc) superfamily.

2D gel databases

SWISS-2DPAGEP75797.

Proteomic databases

EPDiP75797.
PaxDbiP75797.
PRIDEiP75797.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC73917; AAC73917; b0830.
BAA35525; BAA35525; BAA35525.
GeneIDi945459.
KEGGiecj:JW5111.
eco:b0830.
PATRICi32116863. VBIEscCol129921_0857.

Organism-specific databases

EchoBASEiEB3246.
EcoGeneiEG13473. gsiB.

Phylogenomic databases

eggNOGiENOG4105DEF. Bacteria.
COG0747. LUCA.
HOGENOMiHOG000164955.
InParanoidiP75797.
KOiK13889.
OMAiKESPWVP.
PhylomeDBiP75797.

Enzyme and pathway databases

BioCyciEcoCyc:YLIB-MONOMER.
ECOL316407:JW5111-MONOMER.
MetaCyc:YLIB-MONOMER.

Miscellaneous databases

EvolutionaryTraceiP75797.
PROiP75797.

Family and domain databases

InterProiIPR030678. Peptide/Ni-bd.
IPR023765. SBP_5_CS.
IPR000914. SBP_5_dom.
[Graphical view]
PfamiPF00496. SBP_bac_5. 1 hit.
[Graphical view]
PIRSFiPIRSF002741. MppA. 1 hit.
PROSITEiPS01040. SBP_BACTERIAL_5. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiGSIB_ECOLI
AccessioniPrimary (citable) accession number: P75797
Secondary accession number(s): Q9R7R5, Q9R7R7, Q9R7R8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: February 1, 1997
Last modified: November 2, 2016
This is version 130 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.