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Protein

Glutathione import ATP-binding protein GsiA

Gene

gsiA

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Part of the ABC transporter complex GsiABCD involved in glutathione import. Responsible for energy coupling to the transport system.1 Publication

Enzyme regulationi

Inhibited by verapamil but not by carbonyl cyanide m-chlorophenylhydrazone (CCCP).1 Publication

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi49 – 56ATPPROSITE-ProRule annotation8
Nucleotide bindingi357 – 364ATPPROSITE-ProRule annotation8

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHydrolase
Biological processTransport
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciEcoCyc:YLIA-MONOMER
MetaCyc:YLIA-MONOMER

Protein family/group databases

TCDBi3.A.1.5.11 the atp-binding cassette (abc) superfamily

Names & Taxonomyi

Protein namesi
Recommended name:
Glutathione import ATP-binding protein GsiA (EC:3.6.3.-)
Gene namesi
Name:gsiA
Synonyms:yliA
Ordered Locus Names:b0829, JW5897
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi

Organism-specific databases

EcoGeneiEG13472 gsiA

Subcellular locationi

GO - Cellular componenti

  • integral component of plasma membrane Source: EcoCyc
  • plasma membrane Source: EcoCyc

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000931951 – 623Glutathione import ATP-binding protein GsiAAdd BLAST623

Proteomic databases

PaxDbiP75796
PRIDEiP75796

Interactioni

Subunit structurei

The complex is composed of two ATP-binding proteins (GsiA), two transmembrane proteins (GsiC and GsiD) and a solute-binding protein (GsiB).Curated

Protein-protein interaction databases

BioGridi4259981, 709 interactors
STRINGi316385.ECDH10B_0898

Structurei

3D structure databases

ProteinModelPortaliP75796
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini15 – 269ABC transporter 1PROSITE-ProRule annotationAdd BLAST255
Domaini314 – 564ABC transporter 2PROSITE-ProRule annotationAdd BLAST251

Sequence similaritiesi

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiENOG4108JQ6 Bacteria
COG1123 LUCA
InParanoidiP75796
KOiK13892
OMAiFQLTEAI
PhylomeDBiP75796

Family and domain databases

InterProiView protein in InterPro
IPR003593 AAA+_ATPase
IPR003439 ABC_transporter-like
IPR017871 ABC_transporter_CS
IPR013563 Oligopep_ABC_C
IPR027417 P-loop_NTPase
PfamiView protein in Pfam
PF00005 ABC_tran, 2 hits
PF08352 oligo_HPY, 2 hits
SMARTiView protein in SMART
SM00382 AAA, 2 hits
SUPFAMiSSF52540 SSF52540, 2 hits
PROSITEiView protein in PROSITE
PS00211 ABC_TRANSPORTER_1, 2 hits
PS50893 ABC_TRANSPORTER_2, 2 hits

Sequencei

Sequence statusi: Complete.

P75796-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPHSDELDAG NVLAVENLNI AFMQDQQKIA AVRNLSFSLQ RGETLAIVGE
60 70 80 90 100
SGSGKSVTAL ALMRLLEQAG GLVQCDKMLL QRRSREVIEL SEQNAAQMRH
110 120 130 140 150
VRGADMAMIF QEPMTSLNPV FTVGEQIAES IRLHQNASRE EAMVEAKRML
160 170 180 190 200
DQVRIPEAQT ILSRYPHQLS GGMRQRVMIA MALSCRPAVL IADEPTTALD
210 220 230 240 250
VTIQAQILQL IKVLQKEMSM GVIFITHDMG VVAEIADRVL VMYQGEAVET
260 270 280 290 300
GTVEQIFHAP QHPYTRALLA AVPQLGAMKG LDYPRRFPLI SLEHPAKQAP
310 320 330 340 350
PIEQKTVVDG EPVLRVRNLV TRFPLRSGLL NRVTREVHAV EKVSFDLWPG
360 370 380 390 400
ETLSLVGESG SGKSTTGRAL LRLVESQGGE IIFNGQRIDT LSPGKLQALR
410 420 430 440 450
RDIQFIFQDP YASLDPRQTI GDSIIEPLRV HGLLPGKDAA ARVAWLLERV
460 470 480 490 500
GLLPEHAWRY PHEFSGGQRQ RICIARALAL NPKVIIADEA VSALDVSIRG
510 520 530 540 550
QIINLLLDLQ RDFGIAYLFI SHDMAVVERI SHRVAVMYLG QIVEIGPRRA
560 570 580 590 600
VFENPQHPYT RKLLAAVPVA EPSRQRPQRV LLSDDLPSNI HLRGEEVAAV
610 620
SLQCVGPGHY VAQPQSEYAF MRR
Length:623
Mass (Da):69,114
Last modified:March 6, 2007 - v3
Checksum:iA11DB7EC235F9F76
GO

Sequence cautioni

The sequence BAA35517 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00096 Genomic DNA Translation: AAC73916.2
AP009048 Genomic DNA Translation: BAA35517.2 Different initiation.
PIRiE64820
RefSeqiNP_415350.2, NC_000913.3
WP_001301279.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaiAAC73916; AAC73916; b0829
BAA35517; BAA35517; BAA35517
GeneIDi945457
KEGGiecj:JW5897
eco:b0829
PATRICifig|1411691.4.peg.1449

Entry informationi

Entry nameiGSIA_ECOLI
AccessioniPrimary (citable) accession number: P75796
Secondary accession number(s): Q9R3H8
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: March 6, 2007
Last modified: April 25, 2018
This is version 134 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families

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