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Protein

Glutathione import ATP-binding protein GsiA

Gene

gsiA

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Part of the ABC transporter complex GsiABCD involved in glutathione import. Responsible for energy coupling to the transport system.1 Publication

Enzyme regulationi

Inhibited by verapamil but not by carbonyl cyanide m-chlorophenylhydrazone (CCCP).1 Publication

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi49 – 568ATPPROSITE-ProRule annotation
Nucleotide bindingi357 – 3648ATPPROSITE-ProRule annotation

GO - Molecular functioni

  • ATPase activity, coupled to movement of substances Source: EcoCyc
  • ATP binding Source: UniProtKB-KW

GO - Biological processi

  • glutathione transmembrane transport Source: EcoCyc
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Transport

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciEcoCyc:YLIA-MONOMER.
ECOL316407:JW5897-MONOMER.
MetaCyc:YLIA-MONOMER.

Protein family/group databases

TCDBi3.A.1.5.11. the atp-binding cassette (abc) superfamily.

Names & Taxonomyi

Protein namesi
Recommended name:
Glutathione import ATP-binding protein GsiA (EC:3.6.3.-)
Gene namesi
Name:gsiA
Synonyms:yliA
Ordered Locus Names:b0829, JW5897
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG13472. gsiA.

Subcellular locationi

GO - Cellular componenti

  • plasma membrane Source: EcoCyc
Complete GO annotation...

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 623623Glutathione import ATP-binding protein GsiAPRO_0000093195Add
BLAST

Proteomic databases

PaxDbiP75796.

Interactioni

Subunit structurei

The complex is composed of two ATP-binding proteins (GsiA), two transmembrane proteins (GsiC and GsiD) and a solute-binding protein (GsiB).Curated

Protein-protein interaction databases

BioGridi4259981. 702 interactions.
STRINGi511145.b0829.

Structurei

3D structure databases

ProteinModelPortaliP75796.
SMRiP75796. Positions 12-599.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini15 – 269255ABC transporter 1PROSITE-ProRule annotationAdd
BLAST
Domaini314 – 564251ABC transporter 2PROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 2 ABC transporter domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiENOG4108JQ6. Bacteria.
COG1123. LUCA.
InParanoidiP75796.
KOiK13892.
OMAiESIRVHQ.
OrthoDBiEOG6T7N3V.
PhylomeDBiP75796.

Family and domain databases

Gene3Di3.40.50.300. 2 hits.
InterProiIPR003593. AAA+_ATPase.
IPR003439. ABC_transporter-like.
IPR017871. ABC_transporter_CS.
IPR013563. Oligopep_ABC_C.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF00005. ABC_tran. 2 hits.
PF08352. oligo_HPY. 2 hits.
[Graphical view]
SMARTiSM00382. AAA. 2 hits.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 2 hits.
PROSITEiPS00211. ABC_TRANSPORTER_1. 2 hits.
PS50893. ABC_TRANSPORTER_2. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P75796-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPHSDELDAG NVLAVENLNI AFMQDQQKIA AVRNLSFSLQ RGETLAIVGE
60 70 80 90 100
SGSGKSVTAL ALMRLLEQAG GLVQCDKMLL QRRSREVIEL SEQNAAQMRH
110 120 130 140 150
VRGADMAMIF QEPMTSLNPV FTVGEQIAES IRLHQNASRE EAMVEAKRML
160 170 180 190 200
DQVRIPEAQT ILSRYPHQLS GGMRQRVMIA MALSCRPAVL IADEPTTALD
210 220 230 240 250
VTIQAQILQL IKVLQKEMSM GVIFITHDMG VVAEIADRVL VMYQGEAVET
260 270 280 290 300
GTVEQIFHAP QHPYTRALLA AVPQLGAMKG LDYPRRFPLI SLEHPAKQAP
310 320 330 340 350
PIEQKTVVDG EPVLRVRNLV TRFPLRSGLL NRVTREVHAV EKVSFDLWPG
360 370 380 390 400
ETLSLVGESG SGKSTTGRAL LRLVESQGGE IIFNGQRIDT LSPGKLQALR
410 420 430 440 450
RDIQFIFQDP YASLDPRQTI GDSIIEPLRV HGLLPGKDAA ARVAWLLERV
460 470 480 490 500
GLLPEHAWRY PHEFSGGQRQ RICIARALAL NPKVIIADEA VSALDVSIRG
510 520 530 540 550
QIINLLLDLQ RDFGIAYLFI SHDMAVVERI SHRVAVMYLG QIVEIGPRRA
560 570 580 590 600
VFENPQHPYT RKLLAAVPVA EPSRQRPQRV LLSDDLPSNI HLRGEEVAAV
610 620
SLQCVGPGHY VAQPQSEYAF MRR
Length:623
Mass (Da):69,114
Last modified:March 6, 2007 - v3
Checksum:iA11DB7EC235F9F76
GO

Sequence cautioni

The sequence BAA35517.2 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00096 Genomic DNA. Translation: AAC73916.2.
AP009048 Genomic DNA. Translation: BAA35517.2. Different initiation.
PIRiE64820.
RefSeqiNP_415350.2. NC_000913.3.
WP_001301279.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC73916; AAC73916; b0829.
BAA35517; BAA35517; BAA35517.
GeneIDi945457.
KEGGiecj:JW5897.
eco:b0829.
PATRICi32116861. VBIEscCol129921_0856.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00096 Genomic DNA. Translation: AAC73916.2.
AP009048 Genomic DNA. Translation: BAA35517.2. Different initiation.
PIRiE64820.
RefSeqiNP_415350.2. NC_000913.3.
WP_001301279.1. NZ_LN832404.1.

3D structure databases

ProteinModelPortaliP75796.
SMRiP75796. Positions 12-599.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4259981. 702 interactions.
STRINGi511145.b0829.

Protein family/group databases

TCDBi3.A.1.5.11. the atp-binding cassette (abc) superfamily.

Proteomic databases

PaxDbiP75796.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC73916; AAC73916; b0829.
BAA35517; BAA35517; BAA35517.
GeneIDi945457.
KEGGiecj:JW5897.
eco:b0829.
PATRICi32116861. VBIEscCol129921_0856.

Organism-specific databases

EchoBASEiEB3245.
EcoGeneiEG13472. gsiA.

Phylogenomic databases

eggNOGiENOG4108JQ6. Bacteria.
COG1123. LUCA.
InParanoidiP75796.
KOiK13892.
OMAiESIRVHQ.
OrthoDBiEOG6T7N3V.
PhylomeDBiP75796.

Enzyme and pathway databases

BioCyciEcoCyc:YLIA-MONOMER.
ECOL316407:JW5897-MONOMER.
MetaCyc:YLIA-MONOMER.

Miscellaneous databases

PROiP75796.

Family and domain databases

Gene3Di3.40.50.300. 2 hits.
InterProiIPR003593. AAA+_ATPase.
IPR003439. ABC_transporter-like.
IPR017871. ABC_transporter_CS.
IPR013563. Oligopep_ABC_C.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF00005. ABC_tran. 2 hits.
PF08352. oligo_HPY. 2 hits.
[Graphical view]
SMARTiSM00382. AAA. 2 hits.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 2 hits.
PROSITEiPS00211. ABC_TRANSPORTER_1. 2 hits.
PS50893. ABC_TRANSPORTER_2. 2 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  3. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
    Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
    Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  4. "The yliA, -B, -C, and -D genes of Escherichia coli K-12 encode a novel glutathione importer with an ATP-binding cassette."
    Suzuki H., Koyanagi T., Izuka S., Onishi A., Kumagai H.
    J. Bacteriol. 187:5861-5867(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION IN GLUTATHIONE TRANSPORT, ATPASE ACTIVITY, ENZYME REGULATION.
    Strain: K12 / MG1655 / ATCC 47076.

Entry informationi

Entry nameiGSIA_ECOLI
AccessioniPrimary (citable) accession number: P75796
Secondary accession number(s): Q9R3H8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: March 6, 2007
Last modified: January 20, 2016
This is version 121 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.